JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Zengting Niu,Guotong Sun,Mingtao Tan,Shanchun Yan,Dun Jiang
Heavy metals are prevalent environmental contaminants in pest habitats. This study investigates the tolerance of Hyphantria cunea larvae to β-cypermethrin under cadmium (Cd) stress. Exposure to Cd markedly enhanced larval tolerance to β-cypermethrin. The P450 enzyme was found to be crucial in triggering this cross-tolerance. Notably, Cd exposure significantly upregulated the expression of HcCYP333A29 and HcCYP6AE184 both before and after β-cypermethrin treatment. Silencing these genes reduced the β-cypermethrin tolerance in Cd-exposed larvae. Furthermore, overexpression of HcCYP333A29 and HcCYP6AE184 significantly improved the tolerance of Sf9 cells and Drosophila to β-cypermethrin. Silencing of HcDicer-1, a key gene in the miRNA synthesis pathway, significantly diminished the β-cypermethrin tolerance in Cd-treated larvae. The miR-2739 controlled overexpression of HcCYP333A29, while miR-2854 controlled HcCYP6AE184 overexpression, both of which were essential for β-cypermethrin cross-tolerance in H. cunea larvae under Cd exposure. Collectively, Cd exposure induces cross-tolerance to β-cypermethrin in H. cunea larvae through miRNA-mediated post-transcriptional regulation.
研究领域:
环境毒理学
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分子生物学
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昆虫学
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产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Gaijuan Hou,Yihan Liu,Wenbin Liu,Meiyan Zhang,Huijun Ding,Wenguang Zhou
Whether antibiotic resistance driven by environmental pollutant represents a transient ecological response or a permanent evolutionary shift remains a critical knowledge gap in global health. Here, we tracked the antibiotic tolerance evolution of the primary producer Synechocystis sp. PCC 6803 under chronic exposure (140 d) to environmentally relevant concentrations of copper [Cu(II): 250 μg/L] and the fluoroquinolone antibiotic moxifloxacin [MOX: 20 μg/L], respectively. The evolved cyanobacterial populations acquired high-level tolerance without obvious fitness costs. Notably, this tolerance exhibited dual characteristics: multidrug cross-tolerance was transient and fully reversible upon stressor removal, whereas tolerance to the selective agent itself [Cu(II) or MOX] was stable and heritable. Multi-omics analyses revealed the mechanistic basis for this divergence. Transient tolerance was driven by a highly dynamic form of phenotypic plasticity—manifested as transcriptional reprogramming and large-scale genomic variations that conferred a temporary fitness advantage but were rapidly lost without selective pressures. In contrast, the persistent resistance was cemented by the point mutation in rpoB gene. Our findings demonstrate that pollutants in the environment may drive the antibiotic resistance evolution in cyanobacteria and foster persistent stress tolerance. This highlights the potential risks of primary producers in the aquatic environment as an antibiotic resistance reservoir and vectors for antibiotic resistance dissemination, underscoring the necessity of integrated risk assessment and source control strategies under the “One Health” framework.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Mingtao Tan,Hong Jiang,Rusong Chai,Miao Fan,Zengting Niu,Guotong Sun,Shanchun Yan,Dun Jiang
Heavy metal pollutants are important abiotic environmental factors affecting pest habitats. In this study, Cd pre-exposure significantly increased the tolerance of Lymantria dispar larvae to β-cypermethrin, but did not significantly alter their tolerance to λ-cyhalothrin and bifenthrin. The activation of P450 by Cd exposure is the key mechanism that induces insecticide cross-tolerance in L. dispar larvae. Both before and after β-cypermethrin treatment, Cd exposure significantly increased the expression of CYP6AB224 and CYP6AB226 in L. dispar larvae. Silencing CYP6AB224 and CYP6AB226 reduced the tolerance of Cd-treated L. dispar larvae to β-cypermethrin. Transgenic CYP6AB224 and CYP6AB226 genes significantly increased the tolerance of Drosophila and Sf 9 cells to β-cypermethrin, and the recombinant proteins of both genes could significantly metabolise β-cypermethrin. Cd exposure significantly increased the expression of CnCC and Maf . CnCC was found to be a key transcription factor regulating CYP6AB224 - and CYP6AB226 -activated insecticide cross-tolerance in Cd-treated larvae. Decreasing reactive oxygen species (ROS) levels in the Cd-treated larvae or increasing ROS levels in the untreated larvae reduced or enhanced the expression of CnCC , CYP6AB224 and CYP6AB226 and β-cypermethrin tolerance in L. dispar larvae, respectively. Collectively, Cd exposure confers β-cypermethrin tolerance in L. dispar larvae through the ROS/ CnCC signalling pathway-mediated P450 detoxification. Environmental Implication Heavy metals have been reported to produce cross-tolerance in agricultural insects to insecticides. The growing demand for chemical pest control necessitates expanding research on the widespread occurrence of cross-tolerance to insecticides among pests, particularly forest pests, in areas polluted with heavy metals. In this study, Cd pre-exposure enhanced the cross-tolerance of Lymantria dispar larvae to β-cypermethrin. Cd pre-exposure activated the P450 detoxification system of L. dispar larvae. CYP6AB224 and CYP6AB226 were key detoxify genes to the formation of cross-tolerance. ROS-CnCC signaling pathway was proved to be responsible for activation of detoxify genes.
研究领域:
环境科学
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昆虫学
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分子生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2018
·Vol. 43
·期 45
·20712-20720
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IF 4.229
Biyao Jin,Yiqi Li,Lianhua Zhao
Bimetallic PdV/C nanoparticles have been synthesized by phase-transfer method for catalyzing the oxygen reduction reaction (ORR) in alkaline electrolytes . PdV/C nanoparticles with different V contents are spherical with a mean diameter of 3–4 nm, and the addition of V expands the lattice parameter of Pd as shown by XRD . XPS analysis indicated the binding energy of Pd 0 3d peak increased by ca. 1 eV. Density functional theory (DFT) calculations results shows d-band center of Pd down-shift after V-doping. Based on the electrocatalytic results, introducing V can improve the catalytic activity for ORR, methanol crossover tolerance and stability. Especially, Pd 4 V/C shows higher initial potential (E onset = 1.027 V), excellent methanol crossover tolerance (98.03% retained) and long-term stability (81.30% retained), which are comparable with Pt/C JM . This work provides a new method of synthesizing PdV/C nanoparticles , which have the potential to be used as the cathode electrocatalysts for fuel cells.
研究领域:
材料科学
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电化学
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纳米催化
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能源材料
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燃料电池
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物理化学
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表面科学
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DOI
高影响因子
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