ACS Applied Materials & Interfaces
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2025
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IF 8.2
Ying Yu,Yuan Cheng,Shiwei Xu,Wanyun Song,Mingming Wang,Liqiang Song,Kejia Zhu,Tao Zhang
Noninvasive prenatal diagnosis based on fetal cells (cell-based NIPD) offers a safer alternative to traditional invasive procedures but remains limited by the low abundance of fetal nucleated red blood cells (FNRBCs) in maternal circulation and the inefficiency of current isolation techniques. Here, we present a functionalized interventional system for in situ hematologic cell capture (FISHC) directly from peripheral blood, eliminating the need for blood withdrawal and ex vivo processing. FISHC features a carboxybetaine methacrylate (CBMA)-functionalized medical wire with immobilized FNRBC-specific antibodies, enabling high-specificity cell capture under physiological conditions. In vivo validation in a pregnant monkey model confirms its feasibility for efficient and minimally invasive fetal cell retrieval. FISHC represents a paradigm shift in cell-based NIPD and holds promise for broader biomedical applications, including circulating tumor cell detection and real-time liquid biopsy technologies.
研究领域:
医学工程
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生殖健康
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生物医学工程
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Xie Baogang,Zhan Dujuan,Wu Le,Wang Lele,Lei Yongrong,Liu Meng,Liu Xiaodan,Li Suping
Congenital heart disease (CHD) poses significant clinical challenges due to limitations in early prenatal diagnosis. This study aimed to identify serum metabolic biomarkers for CHD using a combined metabolomics approach. Serum samples from 55 pregnant women carrying fetuses with confirmed CHD (CHDP group) and 49 healthy controls (ZCP group) were analyzed via non-targeted 1 HNMR metabolomics, revealing distinct metabolic profiles. Six choline pathway metabolites were further quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Among these, N,N-dimethylglycine (DMG) exhibited the most significant reduction in the CHDP group (1.07 ± 0.03 vs. 1.55 ± 0.04 µg/mL, p < 0.001), with an area under the ROC curve (AUROC) of 0.883 in the discovery cohort. Validation in an independent cohort (58 CHDP vs. 62 ZCP) confirmed DMG’s diagnostic potential (AUROC = 0.818). While betaine-homocysteine methyltransferase 2 (BHMT2) activity showed no intergroup differences, DMG’s consistent performance highlights its utility as a non-invasive biomarker. This study underscores the clinical value of metabonomics in prenatal CHD screening and establishes DMG as a promising diagnostic marker, potentially improving early detection and perinatal management. Graphical abstract
研究领域:
代谢组学
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儿科心脏病学
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产前诊断
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生物医学研究
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PubMed
高影响因子
LAB ON A CHIP
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2023
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IF 6.1
Yulin Ren,Jingcheng Ji,Haoqing Zhang,Lei Cao,Jie Hu,Feng Xu,Zedong Li
Droplet digital polymerase chain reaction (ddPCR) technology has found widespread applications in the ultrasensitive analysis of nucleic acids, where integrated ddPCR platforms with the capability of sample dispersion, followed by in situ amplification and data analysis, are highly expected. However, current integrated ddPCR platforms are usually limited by either difficultly mass-produced materials or lack of integrated control instruments, restricting their practical application. This paper proposes an integrated three-in-one ddPCR platform with high user-friendliness and practicability, which is composed of an easy-to-use chip and a matching control instrument. The chip was made of thermally resistant and easily mass-produced polycarbonate (PC) material, and the benchtop control instrument was designed to perform droplet generation, in situ amplification, and fluorescence reading. The droplet generation and in situ heating on the chip were well characterized. Finally, the performance of the platform was validated through the analysis of the EGFR L858R mutation in lung cancer. The proposed three-in-one ddPCR platform shows great practicability in ultrasensitive nucleic acid testing. By virtue of its sensitivity, practicability, and cost-effectiveness, the ddPCR can serve as a universal detection platform for monitoring nucleic acid in the fields of tumor diagnosis, pathogen detection, and prenatal diagnosis.
研究领域:
生物技术
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分子生物学
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医学诊断
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基因工程
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PubMed
高影响因子
CELL BIOLOGY AND TOXICOLOGY
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2025
·Vol. 41
·期 1
·1-22
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IF 5.9
Meng Xinyue,Wei Xiaowei,Gu Hui,Ma Wei,Liu Dan,Jia Shanshan,Cao Songying,Ding Dongxue,He Yiwen,Huang Wanqi,Xue Jia,Luo Wenting,Sun Wei,Yuan Zhengwei
Duodenal atresia (DA) is a common neonatal digestive tract obstruction, with unclear prenatal diagnostic specificity and optimal intervention timing. tRNA-derived small RNAs (tsRNAs), stable and enriched in blood, are promising biomarkers for disease diagnosis. Therefore, identifying tsRNA biomarkers, elucidating DA pathogenesis, and exploring potential intrauterine interventions is urgently needed. This study conducts tsRNA profiling via sequencing on plasma samples from pregnant women carrying fetuses with DA and matched healthy controls. Validation was performed in 147 pregnant women, including cohorts with fetal gastrointestinal atresia, normal pregnancies, and post-delivery cases. Functional analyses in cellular models and Adriamycin rat models with DA explored the role of key tsRNAs in DA pathogenesis and intrauterine therapy. It is found that tsRNAs, including tRF-61:78-chrM. Leu-TAA, tRF-60:77-Ile-AAT-1-M4, tRF-57:76-Arg-ACG-1-M2, and tRF-58:76-Tyr-GTA-2-M3, were significantly downregulated in DA cases. tRF-58:76-Tyr-GTA-2-M3 is further implicated in DA development, with knockdown inducing excessive apoptosis via upregulation of SUFU and suppression of GLI1, a hedgehog pathway transcription factor. Intraperitoneal microinjection of tRF-58:76-Tyr-GTA-2-M3 agomir in DA rat models reduce apoptosis and mitigates DA formation by modulating SUFU and GLI1 expression. Taken together, this study identifies novel tsRNA biomarkers for DA, with tRF-58:76-Tyr-GTA-2-M3 playing a pivotal role in its pathogenesis. These findings offer insights into DA mechanisms and suggest potential therapeutic targets, advancing strategies for early diagnosis and intervention.
研究领域:
医学
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分子生物学
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产前诊断
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Hui Xu,Jingyin Zhang,Yueshuang Xu,Huan Wang,Fanfan Fu,Qionghua Xu,Yunlang Cai
For giving birth to a healthy baby without Down’s syndrome, screening the level of alpha fetoprotein (AFP) and free-beta human chorionic gonadotropin (β-hCG) in maternal serum has been a routine examination for all gravidas. However, many assays only detect one target once time, which requires large samples and is low-throughput and costly. Herein, we describe a sensitive and inexpensive multiplex detection assay based on inverse opal structure hydrogel barcodes with poly(ethylene glycol) diacrylate (PEG-DA), poly(ethylene glycol) (PEG) and acrylic acid (AA) hybrid components. The polymerized PEG-DA hydrogel could guarantee the stabilities of the inverse opal structure, while the AA could offer active carboxyl groups for the probe immobilization. In addition, the interconnected pores of the inverse opal structure caused by PEG could also provide channels for biomolecules diffusing and reaction into the voids of the barcodes. The immunoassay results of our detection on AFP and β-hCG showed acceptable accuracy and detection reproducibility, and the results were in acceptable agreement with those from common clinic method for the detection of practical clinical samples. Thus, the new platform is apparently suitable for multiplex detection of Down’s syndrome.
研究领域:
生物医学工程
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产前诊断
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材料科学
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高影响因子
危险品化学品经营许可证（带存储）