EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
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2017
·Vol. 119
·期 12
·1700300
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IF 2.145
Long Xu,Li Zhang,Daoming Li,Pengzhan Liu,Chin-Ping Tan,Weifei Wang,Xiangqian Liu,Bo Yang,Dongming Lan,Yonghua Wang
Efficient synthesis of n-3 PUFA-enriched triacylglycerol (TAG) by the esterification of glycerol with n-3 PUFA in deep eutectic solvents (DES) is reported. There was a 1.2-fold increase of TAG yield in DES compared with that in the solvent-free system. Adsorption of the produced water by DES during esterification contributed to enhance the conversion efficiency by changing the reaction equilibrium. DES also served as an effective solvent for enriching the n-3 PUFA of TAG in the upper layer of reaction media. A TAG yield of 55% was achieved under the optimal condition. Practical Applications : Enzymatic synthesis of n - 3 PUFA-enriched triacylglycerol (TAG) is challenged by low yields. Here, deep eutectic solvents show great potential for enhancing the production of n-3 PUFA-enriched TAG. The production of n-3 PUFA-enriched triacylglycerol is greatly enhanced in deep eutectic solvents.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Shuheng Qin,Xiao Cheng,Ziqi Zhou,Xinran Zhang,Jiayang Chen,Peipei Xu,Ting Wu,Yong Hu
Sentinel lymph nodes (SLNs) are pivotal sites for metastatic progression and key indicators of systemic tumor dissemination, with lymphatic metastasis accounting for ∼90% of cancer-related deaths. However, immunotherapy remains largely ineffective, with response rates below 20%, due to the immunosuppressive tumor microenvironment. Here, we present a protein-confined rotor strategy that leverages the supramolecular nanophotosensitizer (BCP3I@M), integrating a Toll-like receptor (TLR7/8) agonist IMDQ and macrophage membrane cloaking for precise SLN targeting. This strategy exploits the protein cavity as a molecular scaffold to constrain the intramolecular motion of the photosensitizer CP, thereby enhancing intersystem crossing efficiency and boosting 1O2 generation by 5.6-fold over ICG. As a result, it significantly amplifies photodynamic therapy (PDT)-induced immunogenic cell death, potentiating antigen presentation and immune activation. Comparative evaluation of two treatment paradigms─primary tumor irradiation (NIR Tum.) versus SLN-directed PDT (NIR T-SLN)─revealed the superior efficacy of the latter in suppressing metastatic dissemination and reshaping the SLN immunosuppressive microenvironment. Moreover, selective IMDQ release further promoted antigen presentation and T cell activation, synergistically reinforcing both innate and adaptive immunity. This strategy not only eradicated lung metastases but also extended survival, offering a clinically translatable approach to precision tumor immunotherapy.
研究领域:
生物化学
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肿瘤学
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免疫学
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药理学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2017
·
IF 7.504
Lifeng Wang,Keming Xu,Xiaochun Hou,Yiyuan Han,Shiying Liu,Christian Wiraja,Cangjie Yang,Jun Yang,Mingfeng Wang,Xiaochen Dong,Wei Huang,Chenjie Xu
The stable presence of fluorophores within the biocompatible and biodegradable elastomer poly(glycerol-co-sebacate) acrylate (PGSA) is critical for monitoring the transplantation, performance, and degradation of the polymers in vivo. However, current methods such as physically entrapping the fluorophores in the polymer matrix or providing a fluorescent coating suffer from rapid leakage of fluorophores. Covalent conjugation of fluorophores with the polymers and the subsequent core-cross-linking are proposed here to address this challenge. Taking rhodamine as the model dye and PGSA nanoparticles (NPs) as the model platform, we successfully showed that the synthesized rhodamine-conjugated PGSA (PGSAR) NPs only released less than 30% rhodamine at day 28, whereas complete release of dye occurred for rhodamine-encapsulated PGSA (PGSA-p-R) NPs at day 7 and 57.49% rhodamine was released out for the un-cross-linked PGSAR NPs at day 28. More excitingly, PGSAR NPs showed a strong quantum yield enhancement (26.24-fold) of the fluorophores, which was due to the hydrophobic environment within PGSAR NPs and the restricted rotation of (6-diethylamino-3H-xanthen-3-ylidene) diethyl group in rhodamine after the conjugation and core-cross-linking. The stable presence of dye in the NPs and enhanced fluorescence allowed a longitudinal tracking of stem cells both in vitro and in vivo for at least 28 days.
研究领域:
生物材料学
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再生医学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2018
·
IF 6.042
Xilin Bai,Suying Xu,Leyu Wang
The sensitive and selective detection of hydrogen sulfide is of great importance due to its crucial role in pathological and physiological processes. Herein, we report a novel fluorescent platform, AuNCs@GC, for selective detection of hydrogen sulfide in living cells by impregnating the Au nanoclusters (AuNCs) into a biocompatible cationic polymer matrix, glycol-chitosan (GC) nanogel. The confinement effect significantly increased the emissive Au(I) units, resulting in a 6-fold enhancement of quantum yield (from 6.38% to 36.42%). In addition, the prepared positively charged AuNCs@GC nanogel exhibits excellent selectivity and improved sensitivity to aqueous sulfides. Moreover, the as-fabricated AuNCs@GC showed very good biocompatibility and super fluorescence stability across the full pH range and good salt tolerance, which demonstrated excellent adaptability toward intracellular sulfide imaging.
研究领域:
分析化学
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材料科学
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生物医学工程
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）