BIORESOURCE TECHNOLOGY
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2021
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IF 9.642
Guanjing Cai,Gefu Zhu,Mingdian Zhou,Nan Lv,Ruming Wang,Chunxing Li,Junjie Li,Xiaofang Pan
Interspecies electron transfer (IET) between syntrophic fatty-acid oxidizing bacteria (SFOBs) and methanogens decided the performance of anaerobic digestion. Electron shuttles, as potential IET accelerators, were controversial concerning their influences on methanogenesis. In this study, concentration-dependent effects of anthraquinone-2-sulfonate (AQS) and cysteine on glucose digestion were firstly demonstrated: low dosage of AQS and cysteine (50 and 100 µM, respectively) had highest methane yield (133.5% and 148.6%, respectively). Using butyrate as substrate, distinct tendencies towards the enrichment of methanogenic community were further revealed. Cysteine just acted as a reductant which lowered ORP quickly and enriched most methanogens. It benefited methanogenesis right until methanogenic substrates accumulated. AQS, however, showed characteristic features of electron shuttles: it was firstly oxidized by SFOBs and then reduced by hydrogenotrophic methanogens, which accelerated methanogenic butyrate degradation. This study showed wide spectrum of SFOBs and methanogens benefited from the addition of electron shuttles, which laid foundation for future application.
研究领域:
环境微生物学
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厌氧消化
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Junjie Li,Chunxing Li,Yanlin Li,Ruming Wang,Mingdian Zhou,Lixin Zhao,Xiaofang Pan,Guanjing Cai,Nan Lv,Jing Ning,Irini Angelidaki,Gefu Zhu
Conductive material assisted anaerobic digestion presented much interest and promising prospect in pollutant removal during wastewater treatment . The present study deeply investigated the effect iron/carbon in anaerobic digestion for dichlorophen (DCP) degradation and methane production in synthetic DCP wastewater treatment. Results showed that nano-zero valent iron/activated carbon (nZVI/AC) and zero valent iron/activated carbon (ZVI/AC) gave higher chemical oxygen demand (COD) conversion (42.18% and 42.61%) and DCP removal (98.49 wt% and 99.00 wt%) in acidification step with better methane production from anaerobic digestion of the pretreated effluent. Same phenomenon occurred in the direct anaerobic degradation process due to the formation of galvanic cells between iron and carbon. In comparison, applying iron/carbon in acidification as pretreatment strategy plus following effluent anaerobic degradation showed higher efficiency in methane production and DCP removal than that of direct anaerobic degradation. Specifically, the methane production was 253.70 mL and 253.41 mL in subsequent anaerobic digestion system after nZVI/AC and ZVI/AC acidification pre-treatment, which was higher than 224.37 mL and 246.31 mL in direct anaerobic digestion system. Microbial community analysis showed that Clostridium_sensu_stricto_1 was the dominated bacteria due to its important role in DCP wastewater treatment. Both acetoclastic and hydrogenotrophic methanogens were enhanced in iron/carbon added systems, which was also agreement with the strengthen in related genes involved in methanogenesis . In conclusion, the present work systematically investigated the enhancement role of iron/carbon system in anaerobic digestion for DCP wastewater treatment and paved way for future application.
研究领域:
环境工程
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污水处理
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微生物学
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厌氧生物技术
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2017
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IF 5.651
Richen Lin,Jun Cheng,Jiabei Zhang,Junhu Zhou,Kefa Cen,Jerry D. Murphy
Interspecies electron transfer between bacteria and archaea plays a vital role in enhancing energy efficiency of anaerobic digestion (AD). Conductive carbon materials (i.e. graphene nanomaterial and activated charcoal) were assessed to enhance AD of ethanol (a key intermediate product after acidogenesis of algae). The addition of graphene (1.0 g/L) resulted in the highest biomethane yield (695.0 ± 9.1 mL/g) and production rate (95.7 ± 7.6 mL/g/d), corresponding to an enhancement of 25.0% in biomethane yield and 19.5% in production rate. The ethanol degradation constant was accordingly improved by 29.1% in the presence of graphene. Microbial analyses revealed that electrogenic bacteria of Geobacter and Pseudomonas along with archaea Methanobacterium and Methanospirillum might participate in direct interspecies electron transfer (DIET). Theoretical calculations provided evidence that graphene-based DIET can sustained a much higher electron transfer flux than conventional hydrogen transfer.
研究领域:
环境微生物学
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生物能工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2025
·
IF 8.4
Mengyuan Zhang,Gaojun Wang,Bo Zhang,Geli Chen,Qian Li,Rong Chen
Surfactants and nanoplastics are emerging contaminants in organic wastewater. Although the influences of surfactants on the anaerobic digestion of organic wastewater were widely investigated and confirmed, the regulation of co-existed surfactants and nanoplastics on syntrophic methanogenesis was not clear. This study explored the inhibitory mechanism of sodium dodecyl sulfate (SDS) surfactant on syntrophic methanogenesis and deciphered how polypropylene (PP) nanoplastics regulating this inhibition. The results showed that individual 200 mg/L SDS dramatically decreased methane yield (P 0 ) by 31.7 % and prolonged the lag time (t 0 ) from 5.2 d to 12.1 d by damaging the cell membrane, disordering extracellular matrix structure, and decreasing cell viability. Meanwhile, the relative abundance of major hydrogenotrophic Methanoculleus decreased by 43.6 %. However, the addition of PP at concentrations between 0.5 and 200 mg/L effectively mitigated the release of intracellular lactate dehydrogenase and enhanced cell viability. Consequently, key parameters P 0 and t 0 recovered to 6.0–20.6 % and 5.0–8.4 d, respectively. This demonstrated an antagonistic effect of PP and SDS co-existence. The physical analysis further suggested that the hydrophobicity of PP acted as carriers for the aggregation of hydrophobic end structure of SDS, which formed surfactant-coated particles. Meanwhile, the exposed hydrophilic head structure of SDS with negative charges had less chance to attach to the cell membrane due to electrostatic repulsion effects, thereby shielded the toxicity of SDS to microbes. These outcomes provided new insights into understanding the role of surfactants and nanoplastics regulating syntrophic methanogenic process.
研究领域:
环境科学
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微生物学
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生态毒理学
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水污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
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2019
·
IF 7.913
Fang Zhang,Wei Zhang,Ding-Kang Qian,Kun Dai,Mark C.M. van Loosdrecht,Raymond Jianxiong Zeng
Sludge , of which alginate-like biomaterial is a major organic component, is an increasing environmental problem. Thus, efficient anaerobic degradation of alginate provides a new method for sludge utilization. In this study, anaerobic alginate hydrolytic bacteria (AHB) were proposed to enrich with methanogens synergetically to reduce the inhibition of intermediate metabolites. The COD of produced methane reached 80.7 ± 1.9% (n = 4) of initial alginate COD. After considering the microbial growth (8%–18% of COD), a good COD balance indicated that alginate was fully consumed and the main final metabolites were methane and CO 2 . Methanogenesis could promote alginate conversion by AHB. The enriched bacteria for alginate degradation in this study were different from that of former known AHB. The metabolic pathway of alginate degradation was revealed by metagenomics , in which oligo-alginate lyase was detected in twelve bacteria, and typical carbon metabolic pathways to convert alginate to methane were identified. More studies of bacterial isolation and biofuel production are still needed in the future.
研究领域:
环境科学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
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2022
·
IF 8.035
Jianan Niu,Xin Kong,Qirui Che,Qingxia Li,Jin Yuan,Jianguo Liu,Yongmei Zhang
Fe 0 above the micro size (zero valent iron; ZVI) can effectively eliminate or inhibit excessive acidification during anaerobic digestion of the organic fraction of municipal solid waste, particularly at high organic loading rates. However, the role of nanoscale ZVI (NZVI) in anaerobic digestion remains controversial. In this study, the effect of NZVI at different dosages (0.4, 0.5, 0.6, 0.7, 0.8 g/g volatile solids [VS]) on the anaerobic digestion stability of the organic fraction of municipal solid waste was investigated and compared with the performance of ZVI, as were its effects on microbial syntrophism. Similarly to ZVI, NZVI recovered methane production from excessive acidification conditions, but the optimal NZVI dosage (0.4 g/gVS) was far lower than that for ZVI (0.8 g/gVS), and eventually the methane yield reached 324 mL/gVS. Microbial structure changes coupled with substance transformation revealed that NZVI enhanced the metabolism of hydrogenotrophic methanogens for interspecies hydrogen transfer and acetotrophic methanogens.
研究领域:
环境工程
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废物管理
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
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2019
·
IF 5.108
Ying Xu,Yiqing Lu,Linke Zheng,Zhiwei Wang,Xiaohu Dai
A sludge structure perspective of the effects of humic matter (HM) on the anaerobic digestion (AD) of sewage sludge was proposed and investigated in this study. It was observed that the net cumulative methane production of sludge was significantly lower in the presence of HM. The fractal dimension values of sludge samples were increased by the addition of HM, whilst their total surface energy values were decreased, indicating that HM reinforced the stability of the sludge structure. Analyses of the physicochemical properties of different sludge samples revealed that HM enhanced the structural stability of sludge organic substances and restricted its organic solubility by interacting with the biopolymers to form a dense granular aggregate, thereby reducing the random-coil degree of extracellular organic substances and its active sites for enzymes, which suggested that HM could inhibit AD by changing the sludge structure. It was further confirmed by the biochemical methane potential (BMP) assay process. The BMP tests and corresponding kinetic analyses revealed that HM can restrict the potentials of sludge organic solubilisation , hydrolysis and acidification processes, but not necessarily the rates of these processes by enhancing the structural stability of high molecular weight readily biodegradable organic matter (RBOM) and restricting the mobility of low molecular weight RBOM (i.e., hydrolysis products). These findings can enrich understanding of the AD of sludge and may thus aid in the development of more effective methods to improve sludge treatment .
研究领域:
环境科学
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微生物学
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污水处理
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Shuang Zhang,Weijie Guan,Haishu Sun,Pan Zhao,Wanqing Wang,Ming Gao,Xiaohong Sun,Qunhui Wang
Microbial electrolysis cell-assisted thermophilic anaerobic digestion (MEC-TAD) is a promising method to improve anaerobic co-digestion efficiency; however, its application is restricted by high energy consumption. To improve the energy use efficiency of MEC-TAD, this study investigated the effect of different intermittent energization strategies on thermophilic co-digestion performance. Results revealed that an 18 h-ON/6h-OFF energization schedule resulted in the fastest electron transfer rate and the highest methane yield (364.3 mL/g VS). Mechanistic analysis revealed that 18 h-ON/6h-OFF resulted in the enrichment of electroactive microorganisms and increased abundance of enzyme-coding genes associated with energy metabolism ( ntp , nuo , atp ), electron transfer ( pilA , nfrA2 , ssuE ), and the hydrogenotrophic methanogenic pathway. Finally, energy balance analysis revealed that 18 h-ON/6h-OFF had the highest net energy benefit (2.52 kJ) and energy conversion efficiency (110.76 %). Therefore, intermittent energization of MEC-TAD using an 18 h-ON/6h-OFF schedule can provide improved performance and more energy savings.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Yonghua Xu,Mukesh Kumar Awasthi,Pengfei Li,Xianghui Meng,Zhi Wang
In this study, ten spent edible fungus (SEF) with different compositional features were used for the maximum methanogenic potential ( P 0 ) evaluation, and the prediction models including regression and kinetics based on this were developed separately. The results showed that the regression model with more chemical components had a good correlation with the P 0 , and at least three chemical compositions could reach the threshold of sensitivity. The Cone model showed the best fitting effect on P 0 in all kinetic models, which had higher R-square (>0.994) and lower error (1.004–5.672%). Meanwhile, the minimum digestive testing time (14 days) was determined by the evaluation of sensitivity via statistical indicators. It is concluded that the determination of the prediction model of P 0 should be evaluated with the combination of statistical indicators and specific requirements.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Mingwei Wang,Zhiqiang Zhao,Yang Li,Song Liang,Yutong Meng,Tengfei Ren,Xiaoyuan Zhang,Yaobin Zhang
Reducing methane emission is of great importance to control the global greenhouse effect. Dissimilatory iron reduction (DIR) coupling of organic matter decomposition may suppress methane production via reducing primary electron donors available for methanogenesis. However, during DIR, the amorphous iron oxides (e.g., ferrihydrite) are easy to transform into more stable crystalline iron minerals, which slowdowns the rate of DIR. Humic substance (HS) with redox activity has been extensively reported to facilitate DIR via “electron shuttles” mechanism, yet little known about the effect of HS on mediating the mineralization of iron oxides and the subsequent influences on DIR and methanogenesis. To clarify this, ferrihydrite and fulvic acid (FA) (as the model substance of HS) were supplied to anaerobic methanogenesis systems. Results showed that FA could significantly decrease the formation of crystalline iron oxides, enhance DIR rate by 13.72% and suppress methanogenesis by 25.13% compared to ferrihydrite supplemented only. By X-ray absorption spectra analysis, it was found that FA could complex with ferrihydrite via forming a Fe-C/O structure on the second shell of Fe atom. Quantum chemical calculation further confirmed that FA reduced the adsorption energy between Fe(II) and ferrihydrite. Our study suggested that rational use of HS to mediate mineralization pathway of iron oxides could efficiently improve the availability of iron oxides to drive DIR and control the conversion of organics into CH 4 in natural or engineered systems.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Weiqi Zhang,Xin Kong,Linlin Bo,Delin Yin,Zhichun Zhang,Xiaojun Fan,Hongping Chen,Jin Yuan
Zeolitic imidazolate framework-8 (ZIF-8) has been widely employed for carbon dioxide (CO 2 ) and methane (CH 4 ) separation, and CO 2 adsorption and storage owing to its high porosity and tunable pore structure. However, its potential to enhance CO 2 -to-CH 4 conversion by grasping CO 2 in hydrogenotrophic methanogenic systems, thereby increasing CH 4 percentage in biogas, remains unexplored. This study synthesized ZIF-8 material and applied it in a biological biogas upgrading system. The results indicated that the CO 2 -to-CH 4 conversion rate was maximized in the system with 0.75 g/L of ZIF-8 added. Over a single reaction cycle (5 days), the CH 4 proportion increased from 60 % to 97 %. Furthermore, in continuous operation over three reaction cycles, the ultimate CH 4 proportion in this reactor consistently exceeded 95 %, meeting the standards of biomethane. The pathway analysis revealed that the ZIF-8 introducing could increase abundance of hydrogenotrophic methanogens and facilitated the transition of metabolic pathway from acetotrophic to hydrogenotrophic methanogenesis.
研究领域:
环境科学
·
微生物学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Chengxin Niu,Teng Cai,Xueqin Lu,Guangyin Zhen,Yang Pan,Xuan Ren,Xi Qin,Wanjiang Li,Yingxiang Tang,Zhongxiang Zhi
Underlying mechanism of continuous nano zero-valent iron (nZVI) supply in functional microbes enrichment and methane bioconversion of waste activated sludge were investigated in a lab-scale continuous stirred tank reactor (CSTR). Results indicated that methane productivity strongly relied on nZVI dosage ( R = 0.998, p (0.05) < 0.001) and leached Fe 2+ concentration ( R = 0.943, p (0.05) = 0.005). The higher nZVI dosage, the higher methane productivity and organic removal were achieved. The methane production rate reached 77.6 ± 9.7 mL/g-VS/d at 160 mg-nZVI/g-TS, increasing by 2.3 times than the phase without nZVI supply. The corrosion/dissociation of nZVI, except for serving as trace element and electron donors (H 2 /[H]), increased system buffering capacity (2835–3410 mg CaCO 3 /L), and decreased oxidation reduction potential from − 152 mV to − 240 mV. Such thermodynamically favorable environment facilitated the proliferation of organics-degrading bacteria ( Firmicutes , Chloroflexi , Smithella , etc.) and hydrogenotrophic methanogen ( Methanobacterium ). As a result, more large organic molecules such as proteins and polysaccharides were hydrolyzed into short-chain fatty acids, thus enhancing the methanogenesis. Furthermore, the continuous supply of nZVI regulated the colloidal properties of sludge flocs by altering particle size distribution and zeta potential via flocculation effect of the released Fe 2+ (136.4 ± 12.1 mg/L). The dewaterability of digested sludge was improved to a certain extent, with capillary suction time dropping to 84.8 ± 4.1 s. This study provided a more systematic understanding of the role of nZVI in promoting methane bioconversion and subsequent digested sludge dewatering and volume reduction, thus expanding the potential application of nZVI in real-world scenarios.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）