APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2013
·Vol. 97
·期 21
·9365-9375
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IF 3.689
Pu Xiang,Qu Xixing,Chen Fei,Bao Jinku,Zhang Guolin,Luo Yinggang
Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida . CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata . Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7.93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4- and 11-fold through their use. A 50- to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPT-producing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.
研究领域:
药用植物学
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微生物学
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药物开发
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DOI
PubMed
高影响因子
Metabolites
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2025
·Vol. 15
·期 1
·45
·
IF 3.7
Zhe Liu,Fengxu Xiao,Yupeng Zhang,Jiawei Lu,Youran Li,Guiyang Shi
Purpose: This study aimed to utilize genetically engineeredBacillus licheniformisfor the production of ergothioneine (EGT). Given the value of EGT and the application ofBacillus licheniformisin enzyme preparation production, we cloned the key enzymes (EanA and EanB) fromChlorbium limicola. Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed inBacillus licheniformisto construct strains. Additionally, we investigated the factors influencing the yield of EGT and made a comparison withEscherichia coli. Methods: The relevant genes were cloned and transferred intoBacillus licheniformis. Fermentation experiments were conducted under different conditions for yield analysis, and the stability of this bacterium was also evaluated simultaneously. Results: The constructed strains were capable of producing EGT. Specifically, the yield of the EanANBN strain reached (643.8 ± 135) mg/L, and its stability was suitable for continuous production. Conclusions: Genetically engineeredBacillus licheniformisdemonstrates potential in the industrial-scale production of EGT. Compared withEscherichia coli, it has advantages, thus opening up new possibilities for the application and market supply of EGT.
研究领域:
微生物学
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生物技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Qiong Zhao,Liqiang Fan,Yanan Cui,Mengxuan Zhao,Chen Deng,Liming Zhao
Chitin oligosaccharides (CHOS) possess potential applications in food, medicine, and agriculture. Nevertheless, their low production efficiency and polymerization degree severely restrict their extensive utilization. In the present study, a Chitinase-producing bacterium was isolated and identified as Chitinilyticum sp.C8. Under the optimized conditions, the Chitinase yield reached 2.24 U/mL. Using extracellular Chitinase derived from Chitinilyticum sp.C8, chitin was converted to CHOS (DP 2–7) and GlcNAc with a yield of 96.7%. Furthermore, the endochitinase ChiC8-2 was isolated from crude enzyme preparation, which can hydrolyze chitin to obtain CHOS (DP 2–6) ((GlcNAc)2 0.2 mg/mL (22.7%), (GlcNAc)3 0.03 mg/mL (3.4%), (GlcNAc)4 0.25 mg/mL (28.4%), (GlcNAc)5 0.09 mg/mL (10.2%), and (GlcNAc)6 0.21 mg/mL (23.9%)) within 30 min. The Chitinilyticum sp.C8 could not only produce various types of chitinases but also pretreat chitin to improve the efficiency of enzymatic hydrolysis. An integrated preparation method from Chitinase production to chitin treatment by Chitinilyticum sp.C8 was developed for the efficient production of CHOS. This study introduces an environmentally friendly strategy for the production of bioactive CHOS, facilitating the sustainable utilization of renewable natural resources.
研究领域:
微生物学
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生物化学
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食品科学
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医药科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
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2025
·Vol. 13
·期 2
·434
·
IF 4.2
Lanwen Zhang,Yongxia Li,Xiaojian Wen,Xuan Wang,Wei Zhang,Dongzhen Li,Yuqian Feng,Zhenkai Liu,Xingyao Zhang
Pine wilt disease (PWD), caused by the pine wood nematode (PWN,Bursaphelenchus xylophilus), is one of the most serious threats to pine forests worldwide. The fungusEsteya vermicola, with its lunate conidia capable of parasitizing the PWN, has shown promise as an efficient biological control agent against PWD. Solid-state fermentation (SSF) is preferred for large-scale applications in the field, as it facilitates microbial agent transport and ensures a long shelf life. However, research on enhancing the yield of lunate conidia fromE. vermicolathrough SSF is limited. In this study, we initially achieved a yield of 3.04 × 108conidia/g using a basic SSF medium composed of wheat bran, corn flour, and soybean flour. To improve this yield, we employed an orthogonal experimental design (OED) to identify the optimal medium composition, which required a wheat bran-to-corn flour-to soybean flour ratio of 7:2:1 (w/w/w), a substrate-to-water ratio of 1:0.7 (w/v), and the addition of 1.33% (w/w) glucose, 1.33% (w/w) yeast extract fermentation, and 1.33% (w/w) MgSO4. Using the response surface methodology (RSM), we calculated the optimal fermentation conditions, which were 24.9 °C, 78.0% relative humidity (RH), an inoculation volume of 16.3% (v/w), and a fermentation time of 7.1 days. Under these conditions, the yield of lunate conidia reached a maximum of 16.58 × 108conidia/g, a 4.45-fold increase after optimization. This study improved the yield ofE. vermicolalunate conidia and provides insights for developing biopesticides based on this strain.
研究领域:
植物病理学
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微生物学
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林业科学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Limin Zhang,Weitao Li,Luxing Wei,Yiming Zhao,Yinghua Qiu,Hanlian Liu,Chuanzhen Huang,Jun Huang
Microfluidic chips have been widely applied in biology and medical research for stably generating uniform droplets that can be solidified into hydrogel microspheres. However, issues such as low microsphere yield, lengthy experimental processes, and susceptibility to environmental interference need to be addressed. In this work, a simple and effective method was developed to modify microfluidic chips at room temperature to improve the production performance of hydrogel microspheres. Numerical simulation-assisted experiments were conducted to comprehensively understand the effect of solution viscosity, hydrophilicity, and flow rate ratio on droplet formation during microsphere production. Chitosan was selected as the main component and combined with poly(ethylene glycol) diacrylate to prepare photocurable hydrogel microspheres as a demonstration. As a result, grafting fluoro-silane (FOTS) increased the contact angle of the channel from 90 to approximately 110°, which led to a 12.2% increase in droplet yield. Additionally, FOTS-modification attenuated the impact of the flow rate ratio on droplet yield by 19.1%. Alternatively, depositing dopamine decreased the channel’s contact angle from 90 to 60°, resulting in a 21.4% increase in particle size and enabling the chip to adjust droplet size over a wider range. Further study demonstrates that the obtained hydrogel microspheres can be modified with layers of aldehyde, which can potentially be used for controlled drug release. Overall, this study proposed a facile method for adjusting the yield and droplet size through surface treatment of microfluidic chips while also enhancing the understanding of the synergistic effects of multiple factors in microfluidics-based microsphere production.
研究领域:
生物工程
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材料科学
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医学研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
·
IF 5.399
Jin-Shu Yang,Tai-Hua Mu,Meng-Mei Ma
The ultrasound-microwave assisted HCl extraction of pectin from potato pulp was optimized using the response surface methodology. Effects of extraction temperature, pH, and time on the yield were evaluated, and structural characteristics of pectin extracted under optimal conditions were determined. The yield was 22.86 ± 1.29% under optimal conditions of temperature 93 °C, pH 2.0, and time 50 min. The obtained pectin was rich in branched rhamnogalacturonan I (61.54 mol%). Furthermore, the pectin was a low-methoxyl (degree of methylation, 32.58%) but highly acetylated (degree of acetylation, 17.84%) pectin and the molecular weight was 1.537 × 10 5 g/mol. Fourier transform infrared spectroscopy and 1 H nuclear magnetic resonance indicated that pectin had a linear region of α-1, 4-linked galacturonic acids which could be methyl and acetyl-esterified, and rhamnose linked with galacturonic acid to form rhamnogalacturonan which was branched with side chains. Scanning electron microscopy showed most of pectin had a lamellae structure.
研究领域:
食品科学
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化学工程
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）