Spectroscopic investigations on the binding of leucomalachite green to bovine serum album
The interaction of bovine serum with leucomalachite green (LMG) was investigated by spectroscopic methods . LMG was found to quench the intrinsic fluorescence of BSA by dynamic quenching. High quenching constant suggested that LMG can form a complex with BSA. Binding constant and number of binding site were determined. The thermodynamic parameters suggested the main interacting force is hydrophobic forces . The effect of LMG on the conformation of BSA was analyzed by synchronous fluorescence spectroscopy . RLS spectra were also used to reconfirm the formation of complex between BSA and LMG.
研究领域:
生物化学
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分子生物学
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光谱学
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药物代谢
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蛋白质-配体相互作用
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）