ANALYST
·
2015
·Vol. 140
·期 13
·4648-4653
·
IF 4.107
Qian Sun,Haiyu Tian,Haoran Qu,Deheng Sun,Zhuo Chen,Liping Duan,Weibing Zhang,Junhong Qian
Two biotinylated coumarin-based fluorescent probes SPS3 and RC3 were designed for differentiating between structurally similar proteins streptavidin (SA) and avidin (AV). A substituted phenyl group is introduced onto SPS3, which may quench the fluorescence through twist intramolecular charge transfer (TICT). The fluorescence of SPS3 is turned on, by restraining the TICT process, when the fluorophore is buried at the surface of SA. RC3 is constructed by incorporating a biotin molecule to a coumarin fluorophore through a 4-atom spacer. The fluorescence intensity of RC3 is enhanced significantly when its fluorophore enters into the less polar binding pocket of AV. SPS3 and RC3 could be applied in distinguishing between SA and AV as well as in fluorescence imaging of biotin receptor over-expressed Hela cells.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yutong Wang,Zhengzheng Wang,Zhongxu Zhan,Ju Liu,Tingting Deng,Hengyi Xu
Staphylococcus aureus is a common foodborne pathogen that can cause a suppurative infection after eating contaminated food. Detection of S. aureus plays an important role in the food industry. In this study, a strategy for the detection of S. aureus using magnetic separation (MS) technology combined with rolling circle amplification (MS-RCA) was proposed. The strategy used antibiotics to capture bacteria and employed RCA products as signal output probes. Vancomycin (Van), as a commonly used antibiotic, can recognize peptidoglycan on the cell wall of Gram-positive bacteria and can effectively identify target bacteria. Therefore, we prepared BSAylated-Van functionalized magnetic beads (Van-MBs) for the pre-enrichment of S. aureus . To ensure the selectivity of this method, we used biotin-pig IgG to bind S. aureus . In addition, to amplify the output signal of the MS-RCA strategy, we introduced streptavidin (SA) and successfully obtained the [email protected] S. aureus @biotin-pig [email protected] @biotin-RCA probe complex and used the biotin-avidin-system (BAS) by combining magnetic separation technology and RCA technology to realize the enrichment and specific detection of S. aureus . Furthermore, by optimizing the experimental conditions such as the magnetic separation time and the amount of Van-MBs, the detection performance of this method was improved. Under the optimal conditions, the detection limit of this method for S. aureus was 3.3 × 10 2 CFU/mL in fruit juice, and it was less affected by other bacteria.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Wei Wen,Jing-Yi Huang,Ting Bao,Jun Zhou,Hong-Xing Xia,Xiu-Hua Zhang,Sheng-Fu Wang,Yuan-Di Zhao
We report a triplex signal amplification strategy for sensitive biosensing of cancer biomarker by taking advantage of hairpin-shaped oligonucleotide-functionalized gold nanorods (HO-GNRs), graphene and the avidin-biotin reation. The strategy expands electrochemical detection of carcinoembryonic antigen (CEA) by using an aptamer as biosensor's recognition element and HO-GNRs as signal enhancer. To construct this biosensor, the GNR was used as a carrier of horseradish peroxidase (HRP) and HO aptamer with a biotin at the 3′-end and a thiol at the 5′-end, which amplified the electrochemical response because of a large molar ratio of HRP to HO. In the presence of target CEA, the binding reactions of CEA with the loop portions of the HOs caused HOs’ loop-stem structure opened and exposed the biotins, and then HRP-GNRs-HO conjugates were captured on graphene and streptavidin modified electrodes via the reaction between the exposed biotins and preimmobilized streptavidins. The accumulation of HRP effectively catalyzed the hydrogen peroxide-mediated oxidation of o -phenylenediamine to generate an electrochemical reduction current for CEA detection. Under optimal conditions, the electrochemical biosensor exhibited a wide dynamic range of 5 pg mL −1 and 50 ng mL −1 toward CEA standards with a low detection limit of 1.5 pg mL −1 (signal-to-noise ratio of 3). The proposed biosensor accurately detected CEA concentration in 8 human serum samples from patients with lung diseases, showing excellent correlations with standard chemiluminescence immunoassay . Furthermore, these results of target DNA detection made it abundantly clear that the proposed strategy can also be extended for detection of other relative biomarkers using different functional DNA structures , which shows great prospects in single-nucleotide polymorphisms analysis, biomedical sensing and application for accurate clinical diseases diagnostic.
研究领域:
生物传感器技术
·
医学诊断
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Xuewu Liu,Xingfei Li,Yuxiang Bai,Xing Zhou,Long Chen,Chao Qiu,Cheng Lu,Zhengyu Jin,Jie Long,Zhengjun Xie
This study presents a simple method to immobilize β-agarase onto amino-modified magnetic nanoparticles (MNPs) by biotin/streptavidin (BT/SA) recognition. The effect of BT functionalized with different lengths of poly(ethylene glycol) (PEG) on the activity and stability of heterogeneous biocatalysts was also investigated. The results showed that the enzymatic activity was retained above 93% under the immobilization conditions (pH 7.0, 25 °C, 6 h, and β-agarase concentration of 1 mg/mL). The optimal pH and temperature for both free and immobilized β-agarases were 7 and 30 °C, respectively. PEG length played an important role in the catalytic performance of the immobilized enzyme. As the PEG length increased, an increased trend in activity retention and yield of neoagarobiose was observed. Ultimately, the introduction of PEG24 improved the activity retention of immobilized β-agarase up to 135.67%, and retained 84.18 and 37.04% of residual activity after incubation for 6 h at 40 and 45 °C, respectively, while only 30.50 and 0% of residual activities were observed for free β-agarase at these temperatures, indicating the significantly enhanced stability of the β-agarase after immobilization. Additionally, β-agarase-BTPEG24-SA@MNPs showed the highest yield of neoagarobiose at 45 °C, which was 1.89-fold higher than that of the free enzyme. These results indicated that immobilization of β-agarase on BTPEG-SA@MNPs improved both the catalytic efficiency and stability of the enzyme, thereby promoting the continuous production of neoagarobiose.
研究领域:
酶工程
·
生物催化
·
纳米生物学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2010
·
IF 0.79
Guo-Feng XU,Hui LI,Cui ZHANG,Zi-Yin SUN,Wen-Ying ZHONG,Dan-Ke XU,Hong-Yuan CHEN
We synthesized and prepared a CdTe quantum dot (QD) probe modified with streptavidin (SA) and successfully established a novel visual detection method based on QD coupled with silver enhancement. Combined with the protein microarray analysis technology, this novel method did well in the detection of the reverse-phase protein microarray. To detect the analytical performance of this method, human IgG was used as a mode in this experiment. Initially, the human IgG was immobilized on aldehyde slides, then the optimized bio-goat anti-human IgG and CdTe-SA probe were added, and finally the silver enhancement coloration was held for 15 min; the results showed a good linear correlation between the signal gray level and the logarithm of human IgG immobilized on slides from 100 pg to 1.6 ng with a correlation coefficient ( R 2 ) of 0.997, while its lowest detectable limit was about 50 pg human IgG. This novel method could carry out the sensitive detection of microamounts of protein and shows the characteristics of a simple method that requires very few instruments, and the results obtained using this method can be easily visualized.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Jun Yao
Background The residues of kanamycin can accumulate in the human body for a long time and pose serious health risks, including hearing loss , kidney poisoning, and drug allergic reactions. Therefore, it is crucial to develop a rapid, highly sensitive, and low-cost method for detecting kanamycin residues in foods. However, the current methods have limitations such as low sensitivity, expensive instruments, and multiple steps, which make them impractical for use in resource-limited environments and emergencies. In this study, the creation of a multiple-signal amplification photoelectrochemical biosensor to address these aforementioned issues is discussed. Results Herein, we proposed a multiple signal amplification photoelectrochemical (PEC) biosensor based on carboxylated g-C 3 N 4 and avidin functionalized Ru@SiO 2 for the ultrasensitive detection of kanamycin. The carboxylated g-C 3 N 4 was a highly efficient photoactive substance for amplifying photoelectric signals and a substrate for aptamer immobilization. The DOS and PDOS of g-C 3 N 4 were studied by simulation, and the sensing mechanism of the probe at the molecular level was revealed. Meanwhile, using Ru@SiO 2 as a signal amplifying unit, through the cooperative work between Ru@SiO 2 and g-C 3 N 4 , the photoelectric signal could be double amplified to produce an excellent photocurrent response. Under optimized conditions, the photocurrent response of the PEC biosensor to kanamycin was obtained at concentrations from 0.1 nM to 1000 nM with a lower detection limit of 4.1052 × 10 −11 mol L −1 . This protocol demonstrates high sensitivity, brilliant specific recognition ability, excellent reproducibility, and acceptable stability. Significance The first combination of g-C 3 N 4 and avidin-Ru@SiO 2 as photocurrent materials greatly enhanced the sensitivity of the PEC biosensors. Moreover, the specificity and sensitivity of the PEC biosensor were further improved through the specific interaction between kanamycin and aptamer. The photoelectric conversion mechanism based on g-C 3 N 4 and two pathways for enhancing the photocurrent by Ru(byp) 3 2+ were proposed. Through simulations of the DOS and PDOS of g-C 3 N 4 , the sensing mechanism of the probe at the molecular level was revealed. Under the optimum conditions, the PEC biosensor exhibited a wide linear concentration range and a low detection limit.
研究领域:
食品安全检测
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICAL MICRODEVICES
·
2013
·Vol. 16
·期 2
·237-243
·
IF 2.718
Lei Jian,Lei Chong,Wang Tao,Yang Zhen,Zhou Yong
An investigation of targeted biomolecules was accomplished by combining a micro-fluxgate-based bio-sensing system and Dynabeads. The fluxgate sensor for biomolecule detection was fabricated by Micro Electro-Mechanical system technology, including thick photoresist lithography, electroplating and chemical wet etching. The magnetic core of the sensor was made of Fe-based amorphous ribbon core and three dimension solenoid coils were used as magnetic sensitive elements. The micro-fluxgate-based bio-sensing system was characterized firstly in different concentrations of Dynabeads, and a concentration as low as 100 ng/ml was detected with an external dc magnetic field in the range of 525 μT to 875 μT. Sandwich assays are performed using antibody-antigen pair combination of biotin-streptavidin on a separated Au film substrate surface with a self-assembled layer. Detection of Alpha Fetoprotein antigens with different concentrations was performed and a minimum detectable concentration of 1 pg/ml was achieved by the bio-sensing system. It is of considerable interest due to its potential application in the biomedical field based on known specific binding of target and labels.
研究领域:
生物医学工程
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Yong Lu,Zheng Guo,Jing-Jing Song,Qin-An Huang,Si-Wei Zhu,Xing-Jiu Huang,Yan Wei
A wealth of research has been available discussing nanogap devices for detecting very small quantities of biomolecules by observing their electrical behavior generally performed in dry conditions. We report that a gold nanogapped electrode with tunable gap length for ultra-sensitive detection of streptavidin based on electrochemical impedance technique. The gold nanogap is fabricated using simple monolayer film deposition and in-situ growth of gold nanoparticles in a traditional interdigitated array (IDA) microelectrode . The electrochemical impedance biosensor with a 25-nm nanogap is found to be ultra-sensitive to the specific binding of streptavidin to biotin . The binding of the streptavidin hinder the electron transfer between two electrodes, resulting in a large increase in electron-transfer resistance ( R et ) for operating the impedance. A linear relation between the relative R et and the logarithmic value of streptavidin concentration is observed in the concentration range from 1 pM (picomolar) to 100 nM (nanomolar). The lowest detectable concentration actually measured reaches 1 pM. We believe that such an electrochemical impedance nanogap biosensor provides a useful approach towards biomolecular detection that could be extended to a number of other systems.
研究领域:
生物传感器技术
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yong Chang,Xiaohua Ma,Ting Sun,Lin Liu,Yuanqiang Hao
In the classical heterogeneous electrochemical assay, phosphorylation of peptide substrate is usually performed on the solid-liquid surface. However, immobilization of probe on the solid surface may limit the interaction between the reaction site of probe and the active center of kinase due to the steric hindrance effect. In this work, we proposed a heterogeneous electrochemical method for kinase detection, in which the probe is immobilization-free during the phosphorylation reaction. A biotinylated peptide was used as the kinase substrate. After phosphorylation, the biotinylated phosphopeptide was captured by the neutravidin (NA)-modified electrode through the avidin-biotin interaction. The phosphate groups on the electrode surface were then recognized by the conjugates preformed between biotinylated Phos-tag™ (Bio-tag-Phos) and ferrocene (Fc)-capped NA-modified gold nanoparticle (Fc-AuNP-NA). The method integrates the advantages of homogeneous reaction and heterogeneous detection with high simplicity, sensitivity and specificity. The strategy can be applied to design other heterogeneous biosensors without the immobilization of probe during the enzyme catalyzed reaction .
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 4
·758-766
·
IF 5.047
Dong Han,Baolin Zhang,Chuangang Chong,Cuiping Rong,Jie Tan,Rusen Yang
Effective attachment of magnetic nanoparticles to neuronal membranes has far-reaching significance in activating ion channels and treating neurodegenerative diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) synthesized by the polyol pyrolysis method have the advantages of rich surface functional groups, excellent magnetic properties, controllable particle size and water dispersibility. We propose that perfusion of biotin into the targeted brain area should be initially performed because it tends to be adsorbed by cell membranes, followed by injection of streptavidin (SA)-modified SPIONs into the same area of the brain. By means of the strong binding force between SA and biotin, the SPIONs may subsequently adhere to the cell surfaces in the brain area. In this work, fluorescein isothiocyanate-streptavidin (FITC-SA) was modified on the surface of polyethylene imine (PEI)-SPIONs by the EDC–NHS method and stereotaxically injected into the biotin-supplemented substantia nigra of mice. The combination of fluorescence detection with transmission electron microscopy (TEM) confirmed that FITC-SA/PEI-SPIONs adhered to neuronal membranes in the substantia nigra of mice 24 h after injection. The results show that our strategy can promote the attachment of SPIONs to neuronal membranes.
研究领域:
神经科学
·
纳米技术
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 5
·837-841
·
IF 1.071
Qiao Yujuan,Qian Yue,Liu Mengfei,Liu Nannan,Tang Xingxing
Natural and synthetic nanopores are increasingly popular tools in biosensors. In this work, the DNA su-persandwich structure, which was made from two specially designed probes has been used to be fabricated in solid nanopores. Integrating the idea of affinity between streptavidin and biotin, the DNA supersandwich structure with biotins was successfully constructed for streptavidin detection, and the limitation of detection was found to be 10 fmol/L. This nanodevice allows specific, sensitive and versatile detection of diverse analytes with easy operations, thus we believe that it could be developed to detect some disease-related molecular targets and play a considerable role in biotechnology.
研究领域:
纳米技术
·
生物技术
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED PHYSICS LETTERS
·
2013
·Vol. 103
·期 20
·
IF 3.794
Lei Jian,Lei Chong,Wang Tao,Yang Zhen,Zhou Yong
In this work, a micro-fluxgate-based biosensor was designed for the detection of carcinoembryonic antigen (CEA) labeled by Dynabeads. The sensor with Fe-based amorphous core and three dimension solenoid coils was fabricated by Micro Electro-Mechanical system technology. Sandwich assays are performed using antibody-antigen pair combination of biotin-streptavidin assay on a separated Au film substrate surface with a self-assembled layer. With dc magnetic fields in the range of 560 μ T to 875 μ T, detection of CEAs with different concentrations was performed and a minimum detectable concentration of 1 pg/ml was achieved. Furthermore, CEA samples with different concentrations can be distinguished.
研究领域:
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）