COLLOIDS AND SURFACES B-BIOINTERFACES
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2013
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IF 3.554
Cang Wang,Yan Fan,Meng-Xin Hu,Wei Xu,Jian Wu,Peng-Fei Ren,Zhi-Kang Xu
Glycosylated membrane, as one of the most important affinity membranes, permits affinity separation/purification of proteins based on carbohydrate–protein interactions. It is an important scientific issue to screen facile method for fabricating the glycosylated membrane surface with high glycosyl density. Such a surface can be fabricated by the direct covalent immobilization of carbohydrate ligands on the surfaces of microporous polypropylene membrane (MPPM). First, alkyne-functionalized membrane surface was fabricated by plasma pretreatment combined with UV-induced graft polymerization of 3-(trimethylsilyl) propargyl methacrylate. Then, the glycosylated membrane surface was directly fabricated with the thiol–yne click reaction to ensure rapid process, improved efficiency, and high glycosyl density. Chemical and physical properties of the membrane surface were characterized by ATR/FT-IR, XPS, FESEM and water contact angle measurement. Static lectin adsorption indicates that the glycosylated membrane can specifically adsorb lectin concanavalin A (Con A) other than peanut agglutinin (PNA). Break through curves from dynamic Con A adsorption show the membrane has unique properties such as strong specificity, high adsorption capacity, and reversible binding capability. We suggest that the prepared glycosylated membrane is of great potentials in affinity membrane chromatography for rapid and high-resolution separation/purification of lectins.
研究领域:
生物化学
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材料科学
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分离技术
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2025
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IF 4
Zhenhu Liu,Peijun Ye,Leying Shi,Zheng Zhao,Jingwei Zhou,Ying Zhou,Feng Li,Jia-Huan Qu,Qiqin Wang,Zhengjin Jiang
With the rapid expansion of antibody drug market, most biopharmaceutical industries urgently need to optimize their downstream purification processes to reduce production costs and improve market competitiveness. In this study, a dual-filler polyether sulfone (PES) mixed matrix membrane (MMM) that combines covalent-organic framework (COF) with nano TiO 2 was developed to overcome the drawbacks of conventional protein A based purification methods. Firstly, COF@TiO 2 dual-filler was prepared by Schiff base reaction. The proposed dual-filler MMM was fabricated via nonsolvent-induced phase separation (NIPS), followed by functionalization with a Fab-specific affinity peptide (m-EDPW) of trastuzumab through atom-transfer radical-polymerization method. The resulting m-EDPW@COF@TiO 2 /PES affinity membrane effectively integrates the merits of COF and TiO 2 and show synergistic effects, demonstrating satisfactory hydrophilicity, anti-fouling ability (BSA rejection rate: 97.7%), enrichment recovery (90.8%), binding capacity for trastuzumab (386.6 mg/g), and long-term stability (∼ 21 days). Particularly, this affinity membrane showed good selectivity and specificity, enabling the successful purification of trastuzumab from spiked HCC1937 cancer cell culture medium with satisfactory purity (∼97.4%) and preservation of the antibody secondary structure. This study not only developed a novel affinity membrane with satisfactory antibody separation performance but also opened a new route for developing dual-filler or multi-filler MMM for highly efficient downstream protein purification.
研究领域:
生物工程
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制药科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2017
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IF 6.216
Jinxin Fan,Jianquan Luo,Yinhua Wan
Biocatalytic membranes are promising to remove micro-pollutants in aqueous environment due to their mild and green operation condition. However, more efforts need to be devoted to improving their removal efficiency and stability. In this study, metal chelating affinity membrane chromatography (MCAMC) was used to construct a biocatalytic membrane by selectively capturing laccase from a crude fermentation broth. Metal ions had a significant effect on the activity of the immobilized laccase and copper ion was the best choice. A pH of 4.5 was selected for laccase adsorption and its loading seemed the same under flow rates from 0.5 to 10 mL min −1 thanks to the inherent convective transport of membrane chromatography. The pH value and salt concentration in the storage buffer had an obvious effect on the stability of the immobilized laccase, and the prepared biocatalytic membrane retained 87% of initial activity after 20 days storage. When applying such membrane to micro-pollutant removal (taking bisphenol A (BPA) as an example), a high BPA removal efficiency (99.3%) could be obtained. The biocatalytic membranes could be operated at a high flux of 50 L m −2 h −1 without recycling the permeate into the feed, and its throughput and BPA removal rate were superior to the most results in the literature. However, BPA removal decline (from 99.6% to 56.6% after five cycles) occurred during the successive water treatment due to the membrane fouling caused by BPA polymerization products. Membrane regeneration could be achieved by simple elution-cleaning-reloading, and the laccase activity and BPA removal were fully recovered.
研究领域:
环境生物学
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生物工程
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DOI
高影响因子
RSC Advances
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2016
·Vol. 6
·期 49
·43648-43655
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IF 3.289
Juan Ma,Chaozhan Wang,Yinmao Wei
Membrane separation has notably important applications in many fields. Boronate affinity provides a method to specifically recognize cis-diol-containing molecules. However, there is no report on the preparation and application of boronate affinity membranes. In the present study, high-capacity boronate affinity membranes were synthesized by grafting polyethyleneimine (PEI) to regenerated-cellulose (RC) membranes and subsequently coupling 4-formylphenylboronic acid. Detailed physical characterizations using Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) revealed the success of the synthesis. The morphology of the membranes, which were characterized using an atomic force microscope (AFM), indicates that the membrane became smoother after the polymer was grafted. The boronate affinity membrane had high selectivity and a high adsorption capacity for cis-diol-containing molecules; its saturated adsorption capacity for catechol, salbutamol, dopamine and adenosine was 511, 103, 472 and 120 μmol g−1, respectively, which is much higher than that of most reported particulate or monolithic adsorbents. In addition, the membrane showed notably fast adsorption and desorption equilibration for the cis-diol-containing molecules. It was successfully used to enrich dopamine in urine samples with notably efficient reusability, which indicates its promising application in the pretreatment of real complex samples.
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
COMPOSITES COMMUNICATIONS
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2018
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IF 0
Cheng Duan,Qiuxia Fu,Yang Si,Lifang Liu,Xia Yin,Feng Ji,Jianyong Yu,Bin Ding
Fabricating metal-chelating affinity materials with excellent protein adsorption and isolation properties is of great significance in numerous biotechnological fields, yet still face huge challenges. Herein, we successfully developed a new kind of nanofiber based metal-chelating affinity membranes by orderly modifying the electrospun regenerated cellulose nanofibrous membranes (RC NFMs) with intermediate bridging agent of cyanuric chloride (CC), chelating agent of iminodiacetic acid (IDA) and affinity ligand of Fe 3+ ions. The resultant Fe 3+ ions immobilized RC NFMs (RC-CC-IDA-Fe 3+ NFMs) exhibited a high lysozyme adsorption amount of 365 mg g -1 within the fast equilibrium adsorption time of 4 hours. Meanwhile, the performance of RC-CC-IDA-Fe 3+ NFMs could be facilely regulated by controlling the properties and concentration of protein solutions. Moreover, the obtained nanofibrous affinity materials also possessed superior cycle performance and excellent regeneration ability. Undoubtedly, the successful fabrication of efficient RC-CC-IDA-Fe 3+ NFMs might provide a new kind of affinity adsorbent for protein purification and other potential biotechnological fields.
研究领域:
生物技术
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材料科学
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产品关联
DOI
GREEN CHEMISTRY
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2021
·Vol. 24
·期 1
·238-250
·
IF 10.182
Lei Pan,Shujuan Ma,Ruizhi Tang,Wenrui Wu,Junjie Ou,Cong Li,Yehua Shen
Protein phosphorylation is involved in many biological processes and associated with some diseases. However, because of the low abundance and large dynamic changes of phosphopeptides in biological samples, it is necessary to enrich phosphopeptides before mass spectrometry detection. Many kinds of materials have been developed for phosphopeptide enrichment, but most of them are disposable and their preparation processes are toxic. In this study, a green strategy was presented to fabricate immobilized metal affinity chromatography (IMAC) materials. Using chitosan as a precursor, polyethylene glycol diglycidyl ether as a cross-linking agent and 2,3,4-trihydroxybenzaldehyde as a functional monomer, a series of chitosan membranes (CMs) with free-standing lamellar 3D architectures were prepared via a freeze-casting technique and after chelating with titanium ions (Ti4+) could serve as IMAC adsorbents for phosphopeptide enrichment in biological samples. Due to the stronger chelating force between Ti4+ and the pyrogallol ligand than that between Ti4+ and the phosphate groups, the IMAC material is reusable for the enrichment of phosphopeptides, exhibiting an excellent enrichment performance and a higher capacity than other IMAC materials such as organosilica hybrid monolith and carbonaceous spheres. Besides, four endogenous phosphopeptides and two dephosphorylated fragments were captured by Ti4+-CM from the human serum. Therefore, Ti4+-CM demonstrates potential commercialization prospects because of its green preparation process and reusability.
研究领域:
生物化学
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材料科学
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分析化学
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DOI
高影响因子
FIBERS AND POLYMERS
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2018
·Vol. 19
·期 5
·941-948
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IF 1.353
Li Xiaojie,Chao Guiqun,Wang Liangan,Xu Xianlin,Cai Zhanjun,Shi Lei,Zhuang Xupin,Cheng Bowen
In this work, an affinity nanofiber membrane was successfully prepared by solution blowing of arginine-modified chitosan (CS-Arg) for bovine serum albumin (BSA) adsorption. CS-Arg was firstly synthesized by coupling L-arginine onto chitosan backbone. Then, CS-Arg nanofiber membranes (CANFs) were fabricated using solution blowing process with Polylactide (PLA) as assistant polymer. The results showed that CANFs effectively adsorbed BSA, and the adsorption capacities were influenced by the degrees of substitution (DS) of arginine in CS, pH value, contact time, and initial protein concentration. The highest adsorption capacity of 445.19 mg/g was achieved uvnder the following conditions: DS of 43.7 %, pH of 7.14, and initial concentration of 3.0 mg/ml. BSA adsorbed on the CANFs membrane conformed to Langmuir model, and the adsorption rate was consistent with the second-order kinetics model. This work implies that an arginine-modified chitosan nanofiber-based novel biomaterial has a potential application in adsorption of BSA.
研究领域:
材料科学
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生物技术
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化学工程
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）