Nanoscale
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2019
·Vol. 11
·期 22
·10952-10960
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IF 6.97
Weiwei Huan,Jingshu Zhang,Heng Qin,Fei Huan,Buchuan Wang,Minjie Wu,Jie Li
High-performance affinity materials are highly required in the sample preparation process in mass spectrometry-based glycoproteomics studies. In this research, a novel magnetic nanofiber-based zwitterionic hydrophilic material is prepared for glycopeptide enrichment and identification. The one-dimensional hydroxyapatite nanofiber (HN) acted as the supporting substance for immobilizing both Fe3O4 nanoparticles and Au nanoparticles, following the surface modification with a zwitterionic tripeptide L-glutathione (GSH) via the affinity interactions between the thiol group in GSH and both Au and Fe3O4 to form the magHN/Au-GSH nanofiber. Owing to the unique structural features, excellent hydrophilicity, abundant zwitterionic molecules, and strong magnetic responsiveness, the as-prepared magHN/Au-GSH nanofiber possesses satisfactory specificity for glycopeptide enrichment. As a result, the magHN/Au-GSH nanofiber demonstrated great detection sensitivity (2 fmol), satisfying enrichment recovery (89.65%), large binding capacity (100 mg g−1), and high enrichment selectivity (1 : 100) toward glycopeptides. Furthermore, 246 N-glycosylated peptides corresponding to 104 N-glycosylated proteins were identified from only 1 μL human serum, revealing the great potential of this affinity nanofiber for glycopeptide enrichment and glycoproteomics research.
研究领域:
生物化学
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蛋白质组学
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分析化学
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高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2025
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IF 2.8
Yi Yan,Zifang Peng,Luyan Meng,Wenfen Zhang,Chuan-Fan Ding,Shusheng Zhang
Clarifying the pathogenesis of diabetes contributes to prevent the occurrence of diabetes. Glycosylated proteins carried by serum exosomes are possibly used as biomarkers of some diseases. In this work, a novel hydrophilic carbonaceous sphere was prepared via one-step hydrothermal carbonization (HTC) process by using glucose as raw material, 1,2-epoxy-5-hexene as a linker, 2-aminopurine as hydrophilic functional group, water as the sole solvent (denoted as CS@2AP). The synthesis process was simple and eco-friendly. The obtained CS@2AP proposed high sensitivity (0.08 fmol μL −1 ) and excellent selectivity (1:1000) for glycopeptides enrichment. Moreover, CS@2AP displayed good isolation effect for serum exosomes from diabetes patient analyzed by western blot. Furthermore, when CS@2AP was used for glycopeptides analysis from exosome digests, 68 glycopeptides were specifically captured.
研究领域:
分析化学
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生物化学
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纳米技术
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糖尿病研究
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生物材料
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Weipeng Wu,Zhiyu Chen,Huimin Wang,Xiang An,Lu Cao,Junkai Gao,Yan Chen
The ocean contains abundant water and lithium resources, and extracting lithium from natural seawater is regarded as one of the most effective methods to address the shortage of lithium resources. Adsorption is a highly effective method for capturing lithium. However, traditional adsorption materials are hindered by low capacity, slow kinetics, and poor selectivity. Therefore, this paper developed a novel strategy of solar-driven for enhancing lithium-ion adsorption by synthesizing a new type of adsorbent (ACT-Li). This approach increases the temperature and lithium concentration in the micro-environment at the interface between the adsorbent and seawater, thereby improving both the lithium adsorption capacity and rate while simultaneously generating freshwater from seawater. Experimental results demonstrate that ACT-Li achieves a photothermal conversion efficiency of 97.61 % under single solar irradiation. Furthermore, light significantly increases both the diffusion rate and adsorption capacity of lithium ions, and compared to dark conditions, the adsorption capacity of lithium ions in ACT-Li increased by 35.28 %. In simulated seawater, the adsorption capacity for lithium ions can reach 31.83 mg/g, indicating strong selectivity of ACT-Li for lithium ions. The developed ACT-Li features a simple preparation process, high photothermal conversion efficiency, strong water transport capabilities, and high lithium extraction efficiency from seawater, providing a novel strategy for lithium extraction and opening new avenues for the utilization of marine resources.
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ANALYTICA CHIMICA ACTA
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2019
·
IF 5.256
Peng-Fei Guo,Xi-Ming Wang,Meng-Meng Wang,Ting Yang,Ming-Li Chen,Jian-Hua Wang
The selective adsorption towards glycoproteins from complex biosamples is of vital importance in life science studies. A new zwitterionic hydrophilic material, i.e., a functionalized titanate nanosheet , is prepared by assembling well-dispersed gold nanoparticles (AuNPs) on the surface of ultrathin titanate nanosheets via an ion-exchange approach, followed through immobilizing l -cysteine (L-Cys) by Au-S bonding. This 2D-titanate-based zwitterionic hydrophilic material is shortly termed as L-Cys/Au/TiNSs and it exhibits transverse several hundred nanometers with an ultrathin nanosheet structure. The zwitterionic hydrophilic titanate nanosheets have strong adsorption affinity to glycoproteins, offering a large binding capacity towards immunoglobulin G (1098.9 mg g −1 ), which could be readily stripped into an ammonium hydroxide (NH 4 OH) solution (0.5%, m/v) with a recovery of 82.4%. The practical applications of L-Cys/Au/TiNSs are further proved by successful specific adsorption of IgG from human serum.
研究领域:
材料科学
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生物化学
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生命科学
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高影响因子
ADVANCED ENGINEERING MATERIALS
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2023
·Vol. 25
·期 10
·2201801
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IF 3.6
Xinglinmao Li,Jianglin Gou,Huimeng Feng,Xiao Sun,Xude Qin,Wei Wang,Wen Li,Shougang Chen
The marine coating in the tidal zone is crucial to restoring its protective function in the case of coating defects, mainly due to the structural damage of the coating by the alternation of wet and dry in the tidal zone and the surface damage of the coating by the impact of sand and gravel. Hydrophilic materials such as hydrogel transformed the tidal zone into an entire immersion zone. Due to the complexation of copper, the fouling problem can be effectively reduced. The coating (AAm/Alginate-Ca/Cu-BTA) has excellent mechanical properties (modulus: 200 kPa, toughness 128 J m −2 ) and significant adhesion (138 J m −2 ). Otherwise, herein, simultaneously mechanical tests of hydrogels at different temperatures and excellent mechanical properties (modulus: 160 kPa, toughness 100 J m −2 ) after 7 days of use in alternating dry and wet environments every 6 h are shown. It's proved that the coating has an excellent antifouling and anticorrosion effect. Meanwhile, theoretical study reveals the nature of the weak mechanical properties of hydrogel materials at low temperatures. This work provides an effective strategy for preparing hydrogel coatings with excellent mechanical properties and marine anticorrosion and antifouling. It also proves that hydrogels have broad application prospects in marine tidal zone by excellent water retention performance.
研究领域:
材料科学
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海洋工程
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涂层技术
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生物医学工程
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Advanced Science
·
2019
·Vol. 6
·期 19
·1900531
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IF 15.804
Chenyang Xing,Dazhou Huang,Shiyou Chen,Qichen Huang,Chuanhong Zhou,Zhengchun Peng,Jiagen Li,Xi Zhu,Yizhen Liu,Zhipeng Liu,Houkai Chen,Jinlai Zhao,Jiangqing Li,Liping Liu,Faliang Cheng,Dianyuan Fan,Han Zhang
Herein, a core–shell tellurium–selenium (Te–Se) nanomaterial with polymer-tailed and lateral heterojunction structures is developed as a photothermal absorber in a bionic solar-evaporation system. It is further revealed that the amorphous Se shell surrounds the crystalline Te core, which not only protects the Te phase from oxidation but also serves as a natural barrier to life entities. The core (Te)–shell (Se) configuration thus exhibits robust stability enhanced by 0.05 eV per Se atom and excellent biocompatibility. Furthermore, high energy efficiencies of 90.71 ± 0.37% and 86.14 ± 1.02% and evaporation rates of 12.88 ± 0.052 and 1.323 ± 0.015 kg m −2 h −1 are obtained under 10 and 1 sun for simulated seawater, respectively. Importantly, no salting out is observed in salt solutions, and the collected water under natural light irradiation possesses extremely low ion concentrations of Na + , K + , Ca 2+ , and Mg 2+ relative to real seawater. Considering the tunable electronic structures, biocompatibilities, and modifiable broadband absorption of the solar spectrum of lateral heterojunction nanomaterials of Te–Se, the way is paved to engineering 2D semiconductor materials with supporting 3D porous hydrophilic materials for application in solar desalination, wastewater treatment, and biomedical ventures.
研究领域:
材料科学
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纳米技术
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太阳能
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环境工程
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生物医学工程
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高影响因子
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