SENSORS AND ACTUATORS B-CHEMICAL
·
2016
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IF 4.758
Shulian Bai,Ting Wang,Zhang Zhang,Shangchun Sheng,Wen Yu,Guoming Xie
Adopting the concept and operation of hybridization-based associative toehold activation (HATA) concatemer induced catalyzed hairpin assembly (CHA), a novel colorimetric biosensor was developed for detecting target DNA and human alpha-thrombin. The assemble of separate DNA hairpin components can be triggered by specific target binding that are otherwise unable to spontaneously happen. In the presence of four ingeniously designed hairpin structures, the target molecule can trigger two cascade recycling reactions: HATA concatemer reaction and CHA reaction. In the HATA concatemer reaction, the toehold and branch migration domain overhang at the 5′ end and 3′ end of hairpin probe H1, respectively. Upon the addition of target molecule, plenty of toeholds and branch migration domains can be brought into close proximity via DNA self-assemble. Then, the toeholds and branch migration domains of HATA products serve as the trigger of CHA. In this concatemer recycling, numerous guanine rich DNA duplexes can be produced through the highly effective use of target molecule. The proposed sensing system exhibits a remarkable analytical performance and an ultrahigh discrimination capability for proximal mismatch. Under optimal conditions, the calibration curves have a good linearity from 1 pM to 75 nM and 1 pM to 2.5 nM for target DNA and human alpha-thrombin, respectively. The corresponding detection limits are 0.14 pMand 0.68 pM Therefore, this new biosensor has a great potential for further applications in biomedical research and demonstrates excellent versatility for different analytes.
研究领域:
生物技术
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生物化学
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分子生物学
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分析化学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 414
·期 4
·1553-1561
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IF 4.157
Zhang Wenjuan,Hu Haoyun,Ruan Guihua,Huang Yipeng,Du Fuyou,Chen Zhengyi
In this study, reduced graphene oxide (rGO) hybridized high internal phase emulsions were developed and polymerized as porous carriers for aptamer (5’/5AmMC6/-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3’) modification to enrich human α-thrombin from serum. The structure and properties of the materials were confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscope (FT-IR), and X-ray photoelectron spectra (XPS). The adsorption ability and selectivity were studied and the thrombin was detected with liquid chromatography-mass spectrometry (LC–MS). The adsorption of thrombin onto the sorbent was achieved within 30 min and the desorption was realized using 5.0 mL of acetonitrile/water (80/20, v/v). The thrombin was quantified by LC–MS according to its characteristic peptide sequence of ELLESYIDGR. Graphical abstract
研究领域:
分析化学
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生物材料科学
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生物化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Pei Zou,Yaling Liu,Hongyong Wang,Jun Wu,Feifan Zhu,Hao Wu
Detection of ultralow concentration of specific protein plays a key role in biotechnological applications and medical diagnostics. In this study, we demonstrate an amplified chemiluminescence biosensing platform for sensitive detection of protein. The biosensing platform ingeniously combines target-catalyzed hairpin assembly and Exo III-assisted signal amplification . A catalytic G-quadruplex-hemin DNAzyme is further employed to stimulate the generation of chemiluminescence in the presence of luminol and H 2 O 2 . These designs together allow a high sensitivity for the biotarget, human α-thrombin, resulting in a detection limit of 0.92 pM, and it is much lower than previous reported studies. In addition, the proposed biosensing platform is versatile. By conjugating with various recognition units, it could be employed to sensitive detect various DNA-binding proteins and might find wide applications in biomedical fields .
研究领域:
生物技术
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医学诊断
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生物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Hao Wu,Kai Zhang,Yaling Liu,Hongyong Wang,Jun Wu,Feifan Zhu,Pei Zou
In this work, a new binding-induced and label-free colorimetric method for protein detection has been developed on the basis of an autonomous assembly of hemin/G-quadruplex DNAzyme amplification strategy. The system consists of two proximity probes carrying two aptamer sequences as recognition elements for target, and two hairpin structures include three-fourths and one-fourth of the G-quadruplex sequences in inactive configuration as functional elements. In the presence of target protein, two proximity probes bind to the protein simultaneously, forming a stable DNA–protein complex. Then the complex triggers an autonomous cross-opening of the two functional hairpin structures, leading to the formation of numerous hemin/G-quadruplex DNAzymes. The resulting DNAzymes catalyze the oxidation of colorless 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS 2− ) to the green-colored ABTS •− with the presence of H 2 O 2 , thus providing the amplified colorimetric detection of target. Using human α-thrombin as the protein target, this binding-induced DNAzyme amplification colorimetric method affords high sensitivity with a detection limit of 1.9 pM. Furthermore, this method might be further extended to sensitive detection of other proteins by simply replacing recognition elements of proximity probes.
研究领域:
生物化学
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分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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