EUROPEAN FOOD RESEARCH AND TECHNOLOGY
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2024
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IF 3
Kong Xinxin,Zhang Lingli,Cai Ling,Cheng Shengyu,Li Xi-Ling,Jin Toufeng,Min Jun Zhe
Selenium (Se) is a vital trace element for mammals, but excessive intake can lead to selenium poisoning. To manage Se intake properly, this study developed a novel method using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). This technique allows the simultaneous detection of three selenoamino acids in supplements. The three selenoamino acids were fully separated within 3 min. The method demonstrated excellent linearity (R 2 ≥ 0.9993), high sensitivity (detection limits were 2.49–2.92 fmol), and good precision (intraday and interday precision ranged from 2.87 to 13.54%). This study applied this method to analyze the content of selenoamino acids in organic selenium supplements from various sources and to dynamically monitor their absorption and excretion in the body after consumption. The novel approach offers a new strategy for the quality control of selenium-containing supplements and for studying selenium absorption and metabolism. Graphical abstract
研究领域:
分析化学
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营养学
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毒理学
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产品关联
DOI
高影响因子
TALANTA
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2016
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IF 4.035
Yan-Yun Li,Xiao-Qin Jiang,Ling-Fei Lu,Min Zhang,Guoyue Shi
In this work, we presented a simple, label-free and rapid-responsive fluorescence assay for iodide (I − ) detection based on “molecular beacon (MB)”-hosted thioflavin T (ThT), achieving a limit of detection as low as 158 nM. The proposed method exhibited very good selectivity to I − ions over other anions interference due to the strong binding force between I − ions with Hg 2+ . Upon the addition of I − ions, it would capture Hg 2+ from a T–Hg 2+ –T complex belonging to the MB-like DNA hairpin structure, which eventually quenched the initial fluorescence as output. In addition, it was successfully applied for operation of an integrated DNA logic gate system and to the determination of I − in real samples such as human urine.
研究领域:
分析化学
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生物化学
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分子生物学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2016
·Vol. 39
·期 7
·1347-1356
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IF 2.741
Ting Cheng,Shuqiang Zhu,Bin Zhu,Xiaoyan Liu,Haixia Zhang
The nucleoside or modified nucleoside level in biological fluids reflects the pathological or physiological state of the body. Boronate affinity absorbents are widely used to selectively extract nucleosides from complex samples. In this work, a novel functionalized absorbent was synthesized by attaching 4-mercaptophenylboronic acid to gold nanoparticles on modified attapulgite. The surface of the attapulgite was modified by poly(acryloyloxyethyltrimethyl ammonium chloride) by atom transfer radical polymerization, creating many polymer brushes on the surface. The resultant material exhibited superior binding capacity (30.83 mg/g) for adenosine and was able to capture cis -diol nucleosides from 1000-fold interferences. Finally, to demonstrate its potential for biomolecule extraction, this boronate affinity material was used to preconcentrate nucleosides from human urine and plasma.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Chunjuan Yang,Li Zhang,Mengyuan Li,Xiaoming Lyu,Chaochao Ma,Shuang Jiang,Yue Zhao,Zhibin Wang,Ying Zhang,Yinghe Ji,Xuanyang Ding,Xinhui Huang,Chunli Gan,Xiaotong Wang,Yunpeng Wang,Yang Li,Yanhui Gao
Monitoring anionic pollutants in aquatic environments and biological fluids is essential for safeguarding environmental safety and human health. However, the detection of anions via surface-enhanced Raman scattering (SERS) remains challenging due to their negative charge and weak interaction with traditional substrates. Herein, we present a universal SERS platform based on calcium-induced aggregation of silver nanoparticles (Ag@BOCNPs) that enables sensitive, reproducible, and label-free detection of eight anions—including fluoride, sulfate, phosphate, and nitrate—within complex aqueous and biological matrices. The platform exhibits exceptional performance for eight ions detection, achieving ultralow limits of detection (LODs, S/N = 3) of 0.001 mg/L and limits of quantification (LOQs, S/N = 10) of 0.003 mg/L. The method demonstrates high stability and excellent anti-interference capability, allowing direct fluoride detection in human urine without pretreatment. By integrating SERS spectral data with machine learning algorithms (KNN, RF, SVM, etc.), accurate classification of anion species and quantification of fluoride exposure levels were achieved. This integrated analytical strategy not only addresses a key limitation in anion detection, but also offers a powerful tool for environmental monitoring and human exposure assessment, particularly in regions affected by water contamination and fluorosis risk.
研究领域:
环境科学
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分析化学
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纳米技术
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光谱学
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机器学习
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公共卫生
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
FORENSIC SCIENCE INTERNATIONAL
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2013
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IF 2.307
Liang Meng,Pinjia Meng,Bugang Tang,Qingqing Zhang,Yanji Wang
Based on molecularly imprinted photonic hydrogels (MIPHs) that combined the colloidal-crystal with molecular imprinting technique, a novel label-free colorimetric chemosensor for convenient and fast efficient detection of atropine with high sensitivity and specificity was developed. Due to the special inverse opal arrays with a thin polymer wall in which the imprinted nanocavities of atropine moleculars distributed, the proposed MIPHs designed as water-compatible exhibited high sensitive (as low as 1 pg/mL), rapid responsive (less than 30 s) and specific detection of atropine in complex matrix. The unique three-dimensional, highly-ordered photonic hydrogels would be obviously swelling in response to the specific atropine molecular recognition process and the response would be directly transferred into visually perceptible optical signal (change in color) that could be detected by the naked eye through Bragg diffractive shifts of ordered macroporous arrays. With a broad concentration range varying from 1 pg/mL to 1 μg/mL of atropine, the distinct color changes of MIPHs almost covered the whole visible-light wavelength range from blue to red for semi-quantitative analysis. The smart chemosensor was successfully employed to determine the trace level atropine in human urine samples, providing a fast and effective alternative for semi-quantitative detection of atropine for clinical analysis and forensic investigations.
研究领域:
分析化学
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生物传感器
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材料科学
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临床分析
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法医学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2024
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IF 5.3
Xinyu Wang,Wenbin Wang,Meng Gao,Hao Yu,Min Fu,Wei Chen
A molecularly imprinted electrochemical sensor was designed for the quantitative and efficient detection of melatonin (MT) based on a CuxO/MXene modified screen-printed carbon electrode (MIP/CuxO/MXene/SPCE). Herein, the CuxO/MXene nanocomposite was prepared via a hydrothermal method. The CuxO was uniformly dispersed on the surface and the interlayers of the MXene, forming hybrid heterostructures. The encapsulated CuxO increased the interlayer distance of the MXene matrix to bring more active sites and accelerated the oxidation reaction of melatonin molecules, and thereby the electrochemical response of the sensor was improved. The MIP was constructed by electropolymerization of o-phenylenediamine (o-PD) in the presence of MT on the surface of the CuxO/MXene/SPCE. The morphology and chemical composition of the hybrid film were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy; the electrochemical behaviors of the sensor were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimal conditions, the MIP/CuxO/MXene/SPCE sensor had a wide linear detection range of 5–700 μM and a low detection limit of 0.029 μM (S/N = 3) for MT. The sensor also showed excellent selectivity, reproducibility, and stability. Finally, it was successfully applied for MT detection in human urine with satisfactory recovery. Overall, this study provided a sensitive and efficient strategy for the determination of MT.
研究领域:
分析化学
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纳米技术
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电化学
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生物传感器
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产品关联
DOI
高影响因子
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