RSC Advances
·
2018
·Vol. 8
·期 72
·41368-41375
·
IF 2.936
Liangliang He,Jinjin Xu,Qi Wang,Yezi Zhang,Zifei Qin,Yang Yu,Zhengming Qian,Zhihong Yao,Xinsheng Yao
Shogaols, mainly [6]-shogaol (6S), [8]-shogaol (8S) and [10]-shogaol (10S), the predominant and characteristic pungent phytochemicals in ginger, are responsible for most of its beneficial effects. However, poor oral bioavailability owing to extensive glucuronidation limits their application. The present study aimed to characterize the glucuronidation pathways of 6S, 8S and 10S by using pooled human liver microsomes (HLM), human intestine microsomes (HIM) and recombinant human UDP-glucosyltransferases (UGTs). The rates of glucuronidation were determined by incubating shogaols with uridine diphosphate glucuronic acid-supplemented microsomes. Kinetic parameters were derived by appropriate model fitting. Reaction phenotyping assays, activity correlation analyses and relative activity factors were performed to identify the main UGT isoforms. As a result, one mono-4′-O-glucuronide was detected after incubating each shogaol with HLM and HIM. Enzymes kinetic analysis demonstrated that glucuronidation of shogaols consistently displayed the substrate inhibition profile, and the liver showed higher metabolic activity for shogaols (CLint = 1.37–2.87 mL min−1 mg−1) than the intestine (CLint = 0.67–0.85 mL min−1 mg−1). Besides, reaction phenotyping assays revealed that UGT2B7 displayed the highest catalytic ability (CLint = 0.47–1.17 mL min−1 mg−1) among all tested UGTs. In addition, glucuronidation of shogaols was strongly correlated with AZT glucuronidation (r = 0.886, 0.803 and 0.871 for glucuronidation of 6S, 8S and 10S, respectively; p < 0.01) in a bank of individual HLMs (n = 9). Furthermore, UGT2B7 contributed to 40.8%, 34.2% and 36.0% for the glucuronidation of 6S, 8S and 10S in HLM, respectively. Taken altogether, shogaols were efficiently metabolized through the glucuronidation pathway, and UGT2B7 was the main contributor to their glucuronidation.
研究领域:
药理学
·
生物化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Chunyu Liu,Xingchen Zhao,Liqiong Guo,Qilin Yu,Weifei Zhang,Zijuan Peng,Yan Gao,Xiaoyun Gong,Penghui Li,Hui Jiao,Tao Zhou,Qinghe Zhang,Shanjun Song,Guibin Jiang
With increasing concerns about N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and 6PPD-quinone (6PPD-Q), relevant environmental investigations and toxicological research have sprung up in recent years. However, limited information could be found for human body burden assessment. This work collected and analyzed 200 samples consisting of paired urine and plasma samples from participants (50 male and 50 female) in Tianjin, China. Low detection frequencies (DF, <15 %) were found except for urinary 6PPD-Q (86 %), which suggested the poor residue tendency of 6PPD and 6PPD-Q in blood. The low DFs also lead to no substantial association between two chemicals. Data analysis based on urinary 6PPD-Q showed a significant difference between males and females ( p < 0.05). No significant correlation was found for other demographic factors (Body Mass Index (BMI), age, drinking, and smoking). The mean values of daily excretion (ng/kg bw/day) calculated using urinary 6PPD-Q for females and males were 7.381 ng/kg bw/day (female) and 3.360 ng/kg bw/day (male), and apparently female suffered higher daily exposure. Further analysis with daily excretion and ALT (alanine aminotransferase)/TSH (thyroid stimulating hormone)/ blood cell analysis indicators found a potential correlation with 6PPD-Q daily excretion and liver/immune functions. Considering this preliminary assessment, systematic research targeting the potential organs at relevant concentrations is required.
研究领域:
环境科学
·
毒理学
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DOI
PubMed
高影响因子
Current Pharmaceutical Analysis
·
2021
·Vol. 17
·期 10
·1249-1256
·
IF 0.89
Sun Qian,Yang Yu-fei,Zou Qiao-gen,Xia Yun-yan,Han Cheng-qun
Background: A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method had been developed for the quantification of acotiamide in rat plasma and been applied to pharmacokinetic studies. However, there was no LC-MS/MS method been developed for the determination of acotiamide
in human plasma and its pharmacokinetic study. Objective: A simple and fast LC-MS/MS method was established and validated for the quantification of acotiamide in human Received: plasma and was applied to a pharmacokinetic study. Methods: Sample preparation was accomplished Revised:
Accepted: through protein precipitation, and chromatographic separation was achieved on a Welch, Ultimate XB-C18 column (2.1×50 mm, 3 μm) with a security guard cartridge C18 using a binary gradient with DOI: mobile phase A (Methanol) and B (the solution of 10 mM Ammonium acetate with
0.1% Formic acid) at a flow rate of 400 μL/min. Results: The retention time of acotiamide and its internal standard, acotiamide-d6 was 1.78 min and 1.79 min, respectively. The total run time was 4.0 min. The method was developed and validated over the concentration range of 0.500-100
ng/mL for acotiamide, with correlation coefficient greater than 0.9987. The extraction recovery was more than 108.43% and the matrix effect was not significant. The inter- and intra-day precisions were below 5.80% and accuracies ranged from 92.7 to 103.0%. Acotiamide was demonstrated to be
stable in human plasma under the tested conditions. Conclusion: The validated LC-MS/MS method was successfully applied to study the pharmacokinetic profiles of acotiamide in human plasma after oral administration and has achieved satisfactory results.
研究领域:
药物分析
·
临床药理学
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DOI
高影响因子
Advanced Sensor Research
·
2023
·
IF 0
Tianqing Liu,Danyao Qu,Lihao Guo,Gandong Zhou,Guangjian Zhang,Tao Du,Weiwei Wu
Human respiration reflects abundant physiological information and could enable non-invasive monitoring, providing information about various biological parameters such as respiration rate and depth. The rapidly growing field of humidity sensors bring forward the requirement for good performance. In this work, by virtue of good hydrophilicity and conductivity, a MXene/thermoplastic polyurethane (TPU) composite film is prepared by coating MXene nanosheets on chitosan-modified TPU electrospun nanofibers via electrostatic interactions, for fabricating a humidity sensor. Based on the principle that the tunnel resistance changing with water molecules influences the distance of MXene nanosheets, the MXene/TPU humidity sensor exhibits fast response (12 s), wide humidity response range (11–94% RH), low hysteresis (<7% RH), and excellent repeatability. The humidity sensor can be assembled with a face mask for distinguishing different human respiration patterns and accurately monitoring respiratory signals during different physical activities, suggesting its promising applications in the fields of respiratory monitoring.
研究领域:
材料科学
·
生物医学工程
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DOI
Micromachines
·
2021
·Vol. 12
·期 6
·602
·
IF 2.891
Mingran Liu,Yang Liu,Limin Zhou
With the development of human healthcare devices, smart sensors, e-skins, and pressure sensors with outstanding sensitivity, flexibility, durability and biocompatibility have attracted more and more attention. In this paper, to develop a novel flexible pressure sensor with high sensitivity, different poly (vinylidene fluoride-trifluoroethylene) (PVDF-TrFE)-based composite membranes were fabricated, characterized and tested. To improve the β-phase crystallinity and piezoelectricity of the membranes, and for the purpose of comparison, nano ZnO particles with different concentrations (99:1, 9:1 in a weight ratio of PVDF-TrFE to ZnO) were, respectively added into PVDF-TrFE polymer acting as a nucleating agent and dielectric material. To facilitate the formation of β-phase crystal, the membranes were fabricated by electrospinning method. After the electrospinning, an annealing process was conducted to the fabricated membranes to increase the size and content of β-phase crystal. Then, the fabricated PVDF-TrFE membranes, acting as the core sensing layer, were, respectively built into multiple prototype sensors in a sandwich structure. The sensitivity of the prototype sensors was tested by an auto-clicker. The stimulation of the auto-clicker on the prototype sensors generated electrical signals, and the electrical signals were collected by a self-built testing platform powered by LabVIEW. As a result, combining the addition of ZnO nanofillers and the annealing process, a highly sensitive pressure sensor was fabricated. The optimal peak-to-peak voltage response generated from the prototype sensor was 1.788 V which shows a 75% increase compared to that of the pristine PVDF-TrFE sensor. Furthermore, a human pulse waveform was captured by a prototype sensor which exhibits tremendous prospects for application in healthcare devices.Keywords:PVDF-TrFE;PVDF-TrFE/ZnO;β phase crystal;human pulsation sensor
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2024
·Vol. 225
·期 22
·2400224
·
IF 2.5
Gen Li,Rongtai Wan,Shuhan Liu,Lina Wang,Mangmang Yu,Jiang Zhong,Hanjun Yang,Ximei Liu,Baoyang Lu
Strain sensors from conducting polymer hydrogel have been widely employed in various wearable devices, electronic skins, and biomedical applications. These sensors provide outstanding flexibility and high sensitivity by integrating conducting polymer with hydrogels, making them particularly suitable for monitoring human motion and physiological signals like heart rate or muscle activity. Despite their extensive application potential, conducting polymer hydrogel face several technical challenges in practical use, including poor mechanical properties, lack of long-term stability, and difficulty in customizable design. This work introduces a method for fabricating a multipedal strain sensor using poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS)/polyvinyl alcohol (PVA) dimethyl sulfoxide (DMSO)hydrogels through screen printing and demonstrates its application in human motion monitoring. The multipedal strain sensor demonstrates a low Young's modulus (200 kPa), high stretchability (400%), and excellent mechanical cyclic stability (3000 cycles). Furthermore, this strain sensor is further applied to detect human movements such as chewing, smiling, fist clenching, arm bending, and carotid pulse monitoring. Comparative analysis between the multipedal-designed sensor and the non-designed sensor highlights the enhanced sensing capabilities of the multipedal sensor. The design of this multipedal sensor holds the potential to broaden the design concepts for strain sensors and offers new insights for wearable devices and electronic skins.
研究领域:
生物医学工程
·
材料科学
·
柔性电子
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DOI
高影响因子
Toxics
·
2025
·Vol. 13
·期 6
·452
·
IF 4.1
Shanshan Ji,Wei Wang,Yong Wang,Hexiang Bai,Zhuo Li,Zongli Huo,Kai Luo
The rapid increase in plastic production and consumption has intensified research into human exposure to micro- and nanoplastics (MNPs) and their health effects. This study quantitatively assessed MNP internal exposure levels in non-invasive human samples, focusing on the four most common types of MNPs (≥300 nm): polyethylene terephthalate (PET), polypropylene (PP), low-density polyethylene (LDPE), and polystyrene (PS). Urine samples from 18 volunteers (4 males, 14 females) were analyzed using pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS) with P(E-13C2) as an internal standard. The study developed a straightforward yet effective analytical approach for quantifying MNPs in biological fluids. MNPs were detected in all urine samples, with concentrations ranging from 0.098 to 0.986 μg/mL and an average concentration of 0.268 ± 0.235 μg/mL. LDPE, 0.074 μg/mL (interquartile range: 0.030–0.243 μg/mL), was the most abundant polymer, accounting for 67.72% of the total MNPs, followed by PS at 21.17%, while PP and PET accounted for 7.06% and 4.05%, respectively. The results also suggest that drinking water type may serve as a distinct source of MNPs in urine. This study provides novel evidence on MNP (≥300 nm) internal exposure in humans and the influence of drinking habits, highlighting the application prospects of this method in assessing the potential health risks of MNPs.
研究领域:
环境科学
·
分析化学
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公共卫生
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DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Ruina Liu,Siming Zhao,Xueke Wu,Zhuojing Zhao,Kangkang Wang,Zhenyu Guo,Aike Xi,Fan Lan,Run Li,Ruihong Liu,Mengzhu Ma,Yonglu Zang,Fei Wang,Qinyuan Jiang,Yunrui Li,Pei Lyu,Rufan Zhang
Radiative cooling is an emerging zero-energy-consumption technology for human body cooling in outdoor scenarios during hot seasons. However, existing radiative cooling textiles are limited by low intrinsic cooling power, high hydrophobicity, and heat-insulating properties, which seriously impede a satisfying cooling effect, perspiration-wicking, and heat dissipation, thus limiting human thermal comfort in practical situations. Here, we developed a radiative cooling meta-fabric that was integrated with high perspiration-wicking and thermal conduction capacity. The meta-fabric included a polyoxymethylene (POM) nanotextile on the front side as a selective radiative emitter, a skin-friendly silicone on the reverse side as a thermal conductor, and patterned bamboo yarns (a cellulose fiber derived from bamboo with excellent hydrophilicity) as the water transport channels. As a result, the meta-fabric could rapidly wick away perspiration (within seconds) and had a high thermal conductivity of 1.5 W/(m·K), exhibiting high-performance human body cooling with a temperature of 10.9 °C lower than the meta-fabric without perspiration. Besides, even without perspiration, the meta-fabric still exhibited a temperature of 9.6 °C lower than commercial cotton fabrics. The work provides an alternative method to design smart textiles for personal thermal management in real applications.
研究领域:
材料科学
·
纺织工程
·
热管理技术
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Li-Li Yao,Jun-Li Wang,Rong-Fa Xu,Ming Zhu,Yan Ma,Bin Tang,Qi-Yuan Lu,Feng-Shan Cai,Xiao Yan,Jing Zheng,Yun-Jiang Yu
Liquid crystal monomers (LCMs) are potentially persistent, bioaccumulating, and toxic substances. However, limited data are available on the occurrence of LCMs in indoor and outdoor air particle matter (PM 10 ) in residential areas. Herein, residential areas near an e-waste dismantling center (Guiyu Town, Shantou City), as well as areas away from the e-waste site (Jiedong District, Jieyang City) were selected as the sampling areas. PM 10 was collected from the indoor environments of Guiyu (IGY) and Jieyang (IJY), as well as those from the outdoor environments (OGY and OJY) using the high-volume air samplers (TH-10000C). The levels of 57 LCMs in PM 10 were analyzed, and the highest concentrations of LCMs were found in IGY (0.970–1080 pg/m 3 ), followed by IJY (2.853–455 pg/m 3 ), OGY (0.544–116 pg/m 3 ) and OJY (0.258–35.8 pg/m 3 ). No significant difference was observed for LCM levels in indoor PM 10 between the two areas ( p > 0.05), which were significantly higher than those in outdoors ( p < 0.05), indicating that the release of electronic products in general indoor environments is a source of LCMs that cannot be ignored. The compositions of LCMs in outdoors were not consistent with those of indoors. The correlation analysis of individual LCMs suggested potential different sources to the LCMs in indoor and outdoor environments. The median daily intake values of Σ 46 LCMs via inhalation were estimated as 0.440, 1.46 × 10 −2 , 0.170 and 1.19 × 10 −2 ng/kg BW/day for adults, and as 2.27, 2.60 × 10 −2 , 0.880 and 2.10 × 10 −2 ng/kg BW/day for toddlers, respectively, indicating much higher exposure doses of LCMs indoors compared with the outdoors, and much higher doses for toddlers compared with adults ( p < 0.05). These results reveal the potentially adverse effects of LCMs on vulnerable populations, such as toddlers, in indoor environments.
研究领域:
环境科学
·
公共卫生
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DOI
PubMed
高影响因子
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
·
2025
·Vol. 47
·期 5
·1-14
·
IF 3.8
Wang Yiyu,Su Huijun,Liu Ning,Yang Naiwang,Gao Pingqiang
Antioxidants have been received recognized as contaminants in the environmental field due to the reports of adverse effects. In this study, a total of 35 outdoor dusts and 20 educational indoor dusts were sampled to investigated the occurrence and spatial distribution of antioxidants, and eight antioxidants were positively found in dusts. For the indoor dusts, the total antioxidant concentrations (Σ 8 Ant) were in the range of 15.7–5282 ng/g, 2,6-di-tert-butyl-4-methylphenol (BHT) was the dominate compound which constituted of 46.4% in the total concentrations of detected antioxidants. The composition profiles of antioxidants (67.9%) in outdoor dusts were different from that in indoor dusts, N -(1,3-dimethylbutyl)- N ′-phenyl-p-phenylenediamine (6PPD) was the main component of all the identified antioxidants. Various microenvironments exhibited different contamination characteristics of antioxidants, the rank order of Σ 8 Ant was following as: campus roads (average concentration: 35.4 ng/g) < pedestrian streets (59.1 ng/g) < urban roads (92.9 ng/g) < teaching buildings (105 ng/g) < laboratory buildings (530 ng/g) < dormitories (1652 ng/g). Based on the measured Σ 8 Ant in dusts, we estimated daily intake via dust ingestion to be 3.90e −2 to 9.55e −2 ng/kg BW/day for adults under high exposure scenario. Overall, the occurrence and spatial distribution of antioxidants in outdoor and educational indoor spaces were investigated and the potential risks of detected antioxidants exposure in toddlers and adults were assessed in the present study.
研究领域:
环境科学
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公共卫生
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Xueke Liu,Peng Wang,Chang Liu,Yiran Liang,Zhiqiang Zhou,Donghui Liu
Beta-cypermethrin (beta-CP), an important pyrethroid insecticide, and its main acid metabolites are frequently detected in human samples. Because beta-CP may pose some risk to human health, we studied dynamics and residues of beta-CP and its metabolites in hen egg, droppings, blood, and 15 other tissues after continuous exposure. A digestive model was then used to study beta-CP’s digestive fate. Beta-CP and its metabolites significantly accumulated in tissues with high lipid contents and were readily transferred to eggs. Beta-CP was mainly metabolized into acid metabolites that accumulated in egg and edible tissues of laying hens, suggesting that humans may be exposed to beta-CP acid metabolites through food.
研究领域:
毒理学
·
食品科学
·
动物科学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Wan Yang,Yunfei Liang,Shengsen Wang,Chen Cai,Xiaozhi Wang,Xiaohu Dai,Xiang Chen
Sewage sludge is a critical reservoir for biological pollutants, and its harmless disposal remains a major issue. Quaternary ammonium compounds (QACs) as typical household disinfectants are inevitably concentrated in sewage sludge, and have the potential to affect human pathogenic bacteria (HPBs) that remain poorly understood. This study found that the relative abundance of HPBs in digesters was decreased by 10 − 20 % at low QACs dose, but increased by 238 − 591 % at high QACs dose. Mechanistic analysis revealed that low QACs doses promoted functional hydrolytic/fermentative bacteria and their metabolism by stimulating extracellular polymeric substances secretion and enhancing resistance to QACs. Conversely, high QAC doses decreased microbial biomass and developed QACs and antibiotic resistance of HPBs by increasing cell membrane permeability and triggering oxidative stress, resulting in deteriorating sanitation performance. These findings provide advanced insights into the potential function and hazards of exogenous QACs on the biosafety of digestate.
研究领域:
环境科学
·
微生物学
·
公共卫生
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）