Evidence-based Complementary and Alternative Medicine
·
2021
·Vol. 2021
·期 1
·9962007
·
IF 2.63
Zhenzhuo Li,Rui Jiang,Jianzeng Liu,Xiaohao Xu,Liwei Sun,Daqing Zhao
Here, we evaluated the in vivo skin-protective effects of topical applications of Panax ginseng C. A. Meyer extract (PG2) and its phenolic acid- (PA-) based components against UVB-induced skin photoaging. PG2 or PA applied to skin of hairless mice after UVB-irradiation alleviated UVB-induced effects observed in untreated skin, such as increased transepidermal water loss (TEWL), increased epidermal thickness, and decreased stratum corneum water content without affecting body weight. Moreover, PG2 and PA treatments countered reduced mRNA-level expression of genes encoding filaggrin (FLG), transglutaminase-1 (TGM1), and hyaluronan synthases (HAS1, HAS2, and HAS3) caused by UVB exposure and reduced UVB-induced collagen fiber degradation by inhibiting the expression of matrix metalloproteinase genes encoding MMP-1, MMP-2, and MMP-9. Meanwhile, topical treatments reduced cyclooxygenase-2 (COX-2) mRNA-level expression in photodamaged skin, leading to the inhibition of interleukin-1 β (IL-1 β ) and interleukin-6 (IL-6) mRNA-level expression. Thus, ginseng phenolic acid-based preparations have potential value as topical treatments to protect skin against UVB-induced photoaging.
研究领域:
药理学
·
皮肤科学
·
植物化学
✓ 已验证
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2017
·Vol. 33
·期 2
·172-178
·
IF 1.024
He Yangfang,Liu Wenlong,Su Rui,Xiu Yang,Pei Jin
The use of direct analysis in real-time(DART) mass spectrometry(MS) for direct detection of saponins and oligosaccharides is difficult mainly because of the strong polarity of these molecules, which results in challenges re-garding desorption and ionization of the compounds. Structure derivatization, such as methylation, is essential for improving the volatility and the proton affinity of saponin and oligosaccharides. In this study, solid-phase methylation of saponins and oligosaccharides was accomplished in a stainless steel methylation column, and the methylated products of astragaloside, salidroside, icariin, ginsenosides(Rb1, Rb2, Rb3, Rd, Re, Rf and Rg1), maltose, isomaltose, sucrose and maltotriose were analyzed using DART-MS. Fragmentations occurred during ionization and tandem mass spectrometry, and most fragments corresponded to the glycosidic bond, cross-ring cleavages, and the neutral loss of CH 3 OH ions, but the aglycone cleavage fragment was not detected. Furthermore, the method was successfully used in the rapid and simultaneous analysis of saponins and oligosaccharides in a ginseng extract; quantitative analysis of ginsenoside Rb1 was also carried out, and consistent content results were obtained in DART-MS and high-performance liquid chromatography(HPLC).
研究领域:
药学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2017
·
IF 3.255
Yang Huang,Tingting Zhang,Yumei Zhao,Haibo Zhou,Guangyun Tang,Marianne Fillet,Jacques Crommen,Zhengjin Jiang
Nucleobases, nucleosides and ginsenosides, which have a significant impact on the physiological activity of organisms, are reported to be the active components of ginseng, while they are less present in ginseng extracts. Few analytical methods have been developed so far to simultaneously analyze these three classes of compounds with different polarities present in ginseng extracts. In the present study, a simple and efficient analytical method was successfully developed for the simultaneous separation of 17 nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The effect of various experimental factors on the separation performance, such as the column type, temperature and backpressure, the type of modifier and additive, and the concentration of make-up solvent were systematically investigated. Under the selected conditions, the developed method was successfully applied to the quality evaluation of 14 batches of ginseng extracts from different origins. The results obtained for the different batches indicate that this method could be employed for the quality assessment of ginseng extracts.
研究领域:
分析化学
·
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）