MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Luxiu Li,Ya Cui,Lingling Ren,Chunyang Li,Yehao Liu,Zhongxiu Jin
In this work, a simple and efficient dispersive solid-phase microextraction (DSPME) method based on magnetic dummy molecularly imprinted polymer nanoparticles (MMIPs) was developed for the extraction of five phthalate monoesters (mPAEs) from human urine samples. The MMIPs synthesized by sol–gel reaction and surface imprinting were analyzed by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometry, and thermogravimetric analysis. The obtained results demonstrate that the MMIPs prepared herein are characterized by high selectivity, good adsorption capacity (124.5 mg/g), excellent reusability (over five sorption–desorption cycles), and fast adsorption rates (about 30 min to equilibrium) with respect to five mPAEs. In addition, the MMIPs can be easily separated from the sample solutions due to their good magnetic susceptibility (34.0 emu/g). Using MMIPs as sorbent material in DSPME, five mPAEs in three human urine samples were extracted, and then analyzed by high-performance liquid chromatography (HPLC). Based on the obtained results, this method shows good linearity in the range of 1–100 mg/L for monomethyl phthalate, monoethyl phthalate, and monobutyl phthalate, in the range of 1–80 mg/L for monobenzyl phthalate, and in the range of 1–50 mg/L for monoethylhexyl phthalate. Moreover, good recovery (80.0%–101.0%) of all five mPAEs was observed at three spiked level with relative standard deviations of 0.6–6.6%. Overall, the results reported herein indicate that the prepared MMIPs can be used as sorbent material for the dispersive solid-phase microextraction of phthalate monoesters in human urine samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Kanagaraj Rajalakshmi,Tongtong Deng,Selvaraj Muthusamy,Meng Xie,Jimin Xie,Kang-Bong Lee,Yuanguo Xu
Citrate is a tricarboxylate, plays vital role in prostate cancer (PC) and the level of citrate is an indicator for PC identification. Herein, triaminoguanidine carbon dots (TAG-CDs) prepared by one step hydrothermal method and used as a citrate receptor. Notably the TAG-CDs without alkaline treatment were highly fluorescent at pH 7 with high quantum yield (11.3%). TAG-CDs were characterized through TEM, XRD, FT-IR, UV–vis and spectrofluorimetry. It is noted that the average size was of 2.8 nm, the presence of highly disordered carbon, retain the functionality of TAG. The absorbance maxima obtained at 294 nm and good emitting response observed at 396 nm. The Y-aromaticity of receptor guanidinium moiety acts as Lewis acid and have peculiar interaction with Lewis base citrate via electrostatic interaction and also protons in the TAG participate hydrogen bonds with citrate, which causes quenching of TAG-CDs. From the obtained linear quenching equation the LOD was found to be 4 nM. The probe expressed high selectivity, high interference tolerance (500 – fold), fast response in 15 mins and good biocompatible. Finally, TAG-CDs utilized for the intracellular imaging of citrate in live MCF-7 cells, it showed good cytotoxicity and delivered contrast images in presence, absence of citrate. TAG-CDs detected the citrate level in human urine samples, the obtained results are validated with HPLC method.
研究领域:
癌症研究
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分析化学
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生物材料
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生物成像
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 8
·1-8
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IF 6.232
Long Yuanli,Liu Shuang,Cai Yunfei,Zhang Jiale,Zhang Xinfeng,Tang Yurong
Graphitic carbon nitride (g-C 3 N 4 ) as an outstanding photoresponsive nanomaterial has been widely used in biosensing. Other than the conventional single channel sensing mode, a triple-channel sensing array was developed for high discrimination of proteins based on the photoresponsive g-C 3 N 4 . Besides the photoluminescence and Rayleigh light scattering features of g-C 3 N 4 , we exploit the new photosensitive colorimetry of g-C 3 N 4 as the third channel optical input. The triple-channel optical behavior of g-C 3 N 4 can be synchronously changed after interaction with the protein, resulting in the distinct response patterns related to each specific protein. Such a triple-channel sensing array is demonstrated for highly discriminative and precise identification of nine proteins (hemoglobin, trypsin, lysozyme, cytochrome c, horseradish peroxidase, transferrin, human serum albumin, pepsin, and myoglobin) at 1 μM concentration levels with 100% accuracy. It also can discriminate proteins being present at different concentration and protein mixtures with different content ratios. The practicability of this sensor array is validated by high accuracy identification of nine proteins in human urine samples. This indicates that the array has a great potential in terms of analyzing biological fluids.
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研究领域:
生物传感器
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分析化学
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材料科学
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生物技术
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PubMed
高影响因子
ANALYST
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2018
·Vol. 144
·期 2
·656-662
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IF 3.864
Shujuan Zhuo,Yuanyuan Guan,Hui Li,Jing Fang,Ping Zhang,Jinyan Du,Changqing Zhu
In this paper, we have presented a novel strategy to fabricate Fe-doped carbon quantum dots (Fe-CQDs) for dopamine sensing applications. The Fe-CQDs are obtained by one step hydrothermal carbonization, using ethylenediamine tetraacetic acid salts and ferric nitrate as the carbon and iron source, which simultaneously incorporates Fe (dopamine-bonding site) and luminescent carbon quantum dots (fluorophores). The added dopamine containing catechol groups might form complexes with Fe ions (doped in CQDs) due to coordination. Subsequently, dopamine was oxidized to generate dopamine-quinone (a known potent electron acceptor) species by ambient O2. Thus, the coordination induced dopamine in proximity to the CQDs, which provided favourable electron acceptors in close proximity to the CQDs and produced high quenching efficiencies. Such fluorescence responses can be used for well quantifying dopamine in the range of 0.01–50 μM with a detection limit of 5 nM (S/N = 3). The proposed sensing system has been successfully used for the assay of dopamine in human urine samples. Preliminary cell image study indicates that the obtained Fe-CQDs possess high photostability and low cytotoxicity, which make them promising for biological applications.
研究领域:
材料科学
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分析化学
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生物医学工程
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生物化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 10
·2121-2129
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IF 3.286
Wu Jia,Xiao Xue,Li Zhenqun,Jia Li
A method of combining magnetic solid-phase separation (MSPE) and chiral capillary electrophoresis (CE) is developed for enantioseparation of trace amounts of β-blockers. Polynorepinephrine-functionalized magnetic nanoparticles (polyNE-MNPs) are synthesized and applied to simultaneously extract three β-blockers (carteolol, metoprolol, and betaxolol). The prepared polyNE-MNPs are spherical with a diameter of 198 ± 17 nm and the thickness of the polyNE coating is about 14 nm. PolyNE possesses abundant catechol hydroxyl and secondary amine groups, endowing the MNPs with excellent hydrophilicity. Under the optimum conditions, the extraction efficiencies of polyNE-MNPs for β-blockers are in the range of 89.6 to 100%, with relative standard deviations (RSDs) below 3.5%. The extraction process can be finished in 4 min. Field-enhanced sample injection (FESI) in chiral CE is constructed to further enhance the sensitivities of β-blocker enantiomers. The limits of detection for β-blocker enantiomers by the FESI-CE with polyNE-MNPs are in the range of 0.401 to 1.59 ng mL −1 . The practicability of this method in real samples is evaluated by analysis of human urine samples. The recoveries for each enantiomer of β-blockers in the real samples range from 89.5 to 92.8%, with RSDs ranging from 0.37 to 5.9%. The whole detection process can be finished in less than 0.5 h. The method demonstrates its great potential in the pharmacokinetic and pharmacodynamic studies of chiral drugs in humans.
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研究领域:
分析化学
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药学
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纳米技术
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DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2023
·Vol. 7
·期 2
·101-109
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IF 4.7
Li Hang,Zhao Chao,Wei Shengnan,Liu Xingxing,Li Juan,Wang Juan
The abnormal changes of biothiols are directly related to health condition of human body, and the effective identification and quantification of biothiols is of great significance for screening and diagnosis of disease. This study described the development of a pH-regulated colorimetric sensor array for discrimination of five kinds of biothiols including cysteine (Cys), glutathione (GSH), homocysteine (Hcy), cysteamine (CA) and N-acetylcysteine (NAC). The proposed sensor array was established using 5-nm- and 20-nm-sized β -cyclodextrin-functionalized gold nanoparticles ( β -CD@AuNPs) as nonspecific receptor and signal transduction elements. Due to the different binding affinity between biothiols and β -CD@AuNPs in various pH environments, the different aggregation behaviors of nanoparticles produced unique colorimetric response patterns, which were able to be distinguished by bare eyes and UV–vis spectrophotometer. Accordingly, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed for pattern recognition and generated a clustering map for a clear differentiation of biothiols at the level of μmol/L. Furthermore, it can be proved that the method was successfully applied to the analysis of biothiols in human urine samples.
研究领域:
分析化学
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生物化学
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纳米技术
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传感器
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2023
·
IF 4.1
Shengdong Pan,Xiaohai Li,Xinwu Xu,Dandan Zhang,Zemin Xu
As production processes have evolved, airborne concentrations of benzene, toluene and xylene in many workplaces are already well below the occupational exposure limits. However, studies have shown that low levels of exposure to benzene, toluene and xylene can still cause health effects in people exposed occupationally. However, there is no literature on health risk assessment of internal exposure. In view of this, an analytical method based on quaternary amine-functionalized core-shell-shell magnetic polymers (QA-CSS-MPs) was developed for the determination of seven metabolites in urine by MSPE-UPLC-DAD-HRMS. Furthermore, an improved QuEChERS method for the extraction of seven metabolites from human urine samples was introduced for the first time and satisfactory extraction rates were achieved. In addition, QA-CSS-MPs microspheres with core-shell-shell structure were designed and synthesized, and the morphology, composition and magnetic properties of the materials were fully characterized to verify the rationality of the synthetic route. Subsequently, QA-CSS-MPs microspheres were used as magnetic solid-phase extraction (MSPE) adsorbents for the purification of urine extracts, and UPLC-DAD-HRMS was used for the detection of seven metabolites. As a result, this method allows the accurate determination of seven metabolites in urine samples over an ultra-wide concentration range (0.001–100 mg/L). Under optimal experimental conditions, i.e., 2% hydrochloric acid in urine for the hydrolysis and 20 mg of QA-CSS-MPs for 5 min purification, the spiked recoveries of the seven target metabolites ranged from 81.5% to 117.7% with RSDs of 1.0%–9.4%. The limits of detection (LODs, S/N≥3) for the established method were in the range of 0.2–0.3 μg/L. The developed method was applied to 254 human urine samples for the determination of seven metabolites. The results showed that the concentration distributions of three xylene metabolites in urine, 2-MHA, 3-MHA, 4-MHA and total MHA, showed statistically significant differences for occupational exposure ( p <0.001). In addition, the results of the internal exposure assessment showed that there is a high potential health risk associated with occupational exposure processes.
研究领域:
分析化学
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职业健康
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环境科学
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材料科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）