JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Wenli Zhu,Jing Zhang,Xiaowan Zhang,Lizhen Han,Peige Qin,Shufang Tian,Qian Zhou,Xuebin Zhang,Minghua Lu
The Al-doped mesoporous crystalline material-41 (Al-MCM-41) composite was prepared and applied as fiber coating material of headspace solid-phase microextraction (HS-SPME) for extraction of polycyclic aromatic hydrocarbons (PAHs) from human urine. Five PAHs including acenaphthene, fluorene, phenanthrene, anthracene, and pyrene are chosen as target analytes to evaluate the performance of the material by GC-FID analysis. The mesoporous Al-MCM-41 composite was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption measurement, and thermogravimetric analysis. The parameters affecting the extraction efficiency of HS-SPME were investigated. Under the optimal conditions, the method exhibits ideal linearity for target analytes in the range of 0.3–600 ng⋅mL -1 with the coefficients (R 2 ) equal or higher than 0.9906. The enrichment factors are calculated from 540 to 1760. The limits of detection (LODs) and limits of quantitation (LOQs) are between the ranges of 0.06–0.18 and 0.3–0.9 ng⋅mL -1 , respectively. The relative standard deviations (RSDs) ( n = 5) of intra-day and inter-day are in the ranges of 1.08–7.49% and 2.84–18.3% respectively. The fiber-to-fiber reproducibility ( n = 3) is in the range of 6.47–13.9%. The method was successfully applied for the analysis of PAHs in human urine with reasonable recoveries which is ranging from 73.29 to 116.1%.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2017
·
IF 0
Jiqing Yang,Xiaoxia Liu,Shucheng Sun,Xin Liu,Li Yang
A facile, cost-effective, and high-throughput screening method was developed for enzyme-based assays based on Robolid/Microplate (RLMP) platform. The RLMP platform is constructed by immobilizing enzyme on commercial robolids and combining it with a standard 96-well microplate to achieve high-throughput analysis. The initiation and quenching of enzymatic reaction can be performed by simply sandwiching or unsealing the enzyme-immobilized robolids and the sample-containing microplate. This platform enables measurements of multiple target analytes simultaneously based on immobilized enzymatic reactions, with analysis time independent of the number of wells in the microplate. Using urea as the model analyte, we have shown that the RLMP platform exhibits large linear detection range of up to 10 mM, fast analysis time of 30 min/96 samples, as well as good reproducibility and stability. Measurements of urea in human urine and serum samples were performed using the RLMP platform and were compared with the commercial urea test kit. A good correlation was found between the two methods. This study shows that the present RLMP platform has promising prospects for detection of clinical markers and application in disease diagnosis and biochemical analysis.
研究领域:
生物技术
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分析化学
·
临床诊断
✓ 已验证
DOI
PubMed
RSC Advances
·
2025
·Vol. 15
·期 33
·26601-26607
·
IF 4.6
Yuqi Dai,Lanmei Zhang,Yuemeng Wang,Anni Fu,Lanling Chu,Lei Zhao
In this work, an integrated method based on the novel packed-fiber solid-phase extraction (PFSPE) combined with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) detection system was proposed for the detection of daidzein, genistein and glycitein in human urine. To efficiently adsorb three kinds of isoflavones in biological fluid, polystyrene-based nanofibers were prepared and used as adsorbents in customized solid phase extraction devices. The composition of the nanofibers, the amount of different salt ions added, the type of eluent, and the interaction of eluent volume, the amounts of nanofibers added, and the extraction time were analyzed to find the optimal adsorption conditions, respectively. The method of optimal extraction conditions for the analysis of three soy isoflavones exhibited low limits of detection (LOD) and limits of quantitation (LOQ) of 1.68–2.74 μg L−1 and 5.09–8.3 μg L−1, respectively, and spiked recovery of 95.2–107.1%, and has been successfully applied to human urine samples. This study demonstrated the potential of PFSPE as methods for the adsorption and detection of daidzein, genistein, glycitein in biological fluid concurrently, providing a valuable tool for pharmacokinetic studies and investigations into the metabolism and bioavailability of isoflavones.
研究领域:
分析化学
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生物分析
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药代动力学
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代谢组学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2022
·Vol. 52
·期 11
·1607-1616
·
IF 2.925
Zhang Yi,Zhang Liwen,Gong Tingcui,Li Tianhao,Li Xuenuan,Feng Jun,Tang Tingfan,Cheng Hao
Due to the highly porous structure, metal organic framework (MOF)-derived materials are extensively applied in the field of electroanalysis. In this experiment, an effective electrochemical sensing based on Co 3 O 4 /C-derived ZIF-67 MOF had been constructed for ultrasensitive determination of quercetin at trace level. The resultant Co 3 O 4 /C porous polyhedron of morphology and nanostructure were carefully examined using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Brunauer–Emmett–Teller (BET) techniques. Differential pulse anodic stripping voltammetry (DPV) method was adopted to analyze quercetin under the optimized conditions. The DPV response of Co 3 O 4 /C film-coated glass carbon electrode (Co 3 O 4 /C/GCE) for quercetin determination was achieved a long linearity ranging from 0.5 to 30 µM, achieving high sensitivity about 1.830 × 10 − 6 A cm − 2 µM − 1 , and the detection limit was calculated to be 0.022 µM (S/N = 3). Besides, the fabricated Co 3 O 4 /C/GCE displayed excellent selectivity, desirable repeatability, and good reproducibility. More importantly, the proposed sensor exhibited satisfactory recovery ranges and accuracy for the determination of trace quercetin in human urine and serum samples, which will make it as an alternative advantageous choice for practical on-site determination. Graphical abstract
The porous Co 3 O 4 /C polyhedron derived by ZIF-67 MOF was successfully synthesized.
The as-synthesized material was employed to prepare chemically modified electrode
for ultrasensitive determination of quercetin. Then the Co 3 O 4 /C-modified glass carbon electrode is displayed good sensitivity and accuracy toward quercetin in urine and
serum samples.
研究领域:
电化学
·
材料科学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）