LUMINESCENCE
·
2019
·Vol. 34
·期 7
·767-773
·
IF 1.691
Chunlei Ma,Wensheng Ren,Jianke Tang,Xiaoli Wang,Dongxin Ji,Rongqian Meng,Cuihong Zhang,Qi Wang
Taking advantage of the compelling properties of d -penicillamine ( d -PA) combined with copper, a method for the sensitive and selective determination of d -PA was established using copper nanocluster (Cu NC)-based fluorescence enhancement. d -PA molecules containing a thiol compound showed a strong tendency to combine with the surface of Cu NCs, causing the re-dispersion of nanoclusters and therefore fluorescence intensity was enhanced. Fluorescence enhancement efficiency of Cu NCs induced by d -PA was linear, with the d -PA concentration varying from 0.6–30 μg ml −1 (R 2 = 0.9952) and with a detection limit of 0.54 μg ml −1 . d -PA content in human urine samples was detected with recoveries of 104.8–112.99%. Fluorescence-enhanced determination of d -PA using Cu NCs was established for the first time and this rapid, easy and sensitive method should attract much attention for this application.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Qiyi He,Xiping Cui,Ding Shen,Yingshan Chen,Zhengyun Jiang,Rui Lv,Sergei A. Eremin,Suqing Zhao
In this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine. Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). The structure effect of tracers on the assay was investigated and the sensitivity of best tracer in the optimized FPIA demonstrated an IC 50 value of 306 ng/mL. The working range of FPIA was 36 ∼ 2 600 ng/mL and the limit of detection (LOD) was 9 ng/mL. The developed FPIA was time-saving that could be completed within 10 min. Human urine samples spiked with GCA were analyzed by this method, followed by confirmation with commercial enzyme immunoassay analysis (EIA). Excellent recoveries and correlation between these two methods were observed ( R 2 = 0.996), suggesting the developed FPIA could be applied to screening of GCA in human urine samples without complicated cleanup.
研究领域:
医学检验
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2013
·Vol. 5
·期 21
·6106-6113
·
IF 1.855
Yajing Lei,Lizheng Fang,Muhammad Sajid Hamid Akash,Zhiming Liu,Weixing Shi,Shuqing Chen
Bisphenol A (BPA) is widely used to manufacture polycarbonate plastics and epoxy resins, which are in widespread use in China. Concerns about potential health impacts from exposure to BPA among the general population are increasing day by day. A sensitive and specific antibody was prepared to develop and compare direct and indirect competitive enzyme-linked immunosorbent assays (ELISAs) for the determination of BPA in human urine. The IC50 value and limit of detection (LOD) for the direct competitive ELISA were 5.5 ng mL−1 and 0.03 ng mL−1 respectively whereas, for the indirect competitive ELISA, the IC50 value and LOD were 7.0 ng mL−1and 0.08 ng mL−1, respectively. We found that the direct competitive ELISA was more specific and sensitive than the indirect competitive ELISA. The established immunoassays were also applied in the determination of BPA in human urine samples. The results from fortified samples and real samples indicated that the direct competitive ELISA was more sensitive and reliable for the measurement of BPA in human urine as compared to the indirect competitive ELISA.
研究领域:
环境健康
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Shuangshou Wang,Zhendong He,Wenzhi Li,Jiayi Zhao,Tong Chen,Shimin Shao,Hongmei Chen
Pipette tip-based solid phase extraction (PT-SPE) has been proved to be an effective and user-friendly separation technique due to its miniaturized procedure and practical convenience. However, the vast majority of existing PT-SPE devices consist of a filter-sorbents-filter sandwich structure, which may suffer the unforeseen risk of sorbents leakage caused by the looseness of filters. More importantly, many high-capacity nanosorbents with particle size smaller than pore size of filters are unavailable. Thus, sorbent packing-free and sample low-consumption PT-SPE could be a more robust strategy for separation and detection, but such a possibility has not been explored yet. Herein we report a tubing reshaping strategy for facile fabrication of sorbent packing-free PT-SPE devices. Three types of reshaped PTs, namely stretched tube-like, self-crimping and filter in-built PTs, were fabricated via simple heating and stretching operations. The reshaped PTs exhibited flexible surface chemical post-modification. The SPE process was directly performed in reshaped PTs with an obviously enhanced extraction efficiency compared to once-shaping PTs while no need of packing sorbents. Extraction of nucleosides from human urine by boronic acid-functionalized reshaped PTs was demonstrated. Our findings technically renovate the structural composition of PT-SPE devices. As PTs are inexpensive and high-plasticity, the sorbent packing-free SPE scheme presented herein could find more promising applications and provides a new perspective for design and fabrication of novel sorbent packing-free SPE devices.
研究领域:
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Langjun Zhou,Yuling Hu,Gongke Li
Conjugated microporous polymers (CMPs), linked by a covalent bond to form an extension of the aromatic ring skeleton, are microporous materials characterized by a highly conjugated structure and high stability. The present study reported on a novel strategy for the synthesis of CMPs with built-in magnetic nanoparticles for excellent enrichment of trace hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in human urine. We modified Fe3O4 nanoparticles with boronic acid groups and then reacted the nanoparticles with reactive monomers of polyphenylene conjugated microporous polymer (PP-CMP) to anchor the magnetic components in the PP-CMP framework. Chemical bonding between Fe3O4 nanoparticles and PP-CMP networks, together with equally firm covalent linkage and rigidity of the PP-CMP network, endows the magnetic PP-CMP with remarkable chemical stability and durability, even in harsh conditions. Magnetic PP-CMP has the characteristics of high conjugation ability, highly porous structure, and magnetism, which makes it an ideal magnetic adsorbent for trace analytes with aromatic conjugation structure. The adsorption mechanism of OH-PAHs on magnetic PP-CMP was investigated and demonstrated that hydrophobic interaction was important for the contribution of interaction between adsorbents and target analytes, together with the assistance of π–π stacking interaction. For the application, the magnetic PP-CMP was used for the enrichment of trace OH-PAHs in human urine of both smokers and nonsmokers in combination with high-performance liquid chromatography with fluorescence detection (HPLC-FLD). It showed good selectivity and excellent sensitivity to these OH-PAHs. Their detection limits were low and in the range of 0.01–0.08 μg·L–1. The OH-PAHs were detected with different amounts from 0.054 to 0.802 μg·L–1 in urine samples from smokers and nonsmokers. The recoveries were found to be 76.0%–107.8%. The results indicate that the magnetic PP-CMP offers an efficient enrichment method for trace OH-PAHs in human urine.
研究领域:
分析化学
·
环境科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2014
·Vol. 78
·期 1
·119-123
·
IF 1.37
Yang Xiujuan,Liu Cui,Chen Zuanguang
A novel and rapid method was developed for the determination of uric acid in human urine by capillary electrophoresis with an improved electromagnetic induction detector. Electrophoretic parameters affecting the separation efficiency, such as buffer composition, buffer pH, buffer concentration, and electroosmotic flow modifier, were systematically investigated. An electrolyte solution consisting of 8.5 mmol L −1 tris(hydroxymethyl)aminomethane (Tris), 1.5 mmol L −1 citric acid, and 0.2 mmol L −1 cetyltrimethylammonium bromide, pH 8.0, was found to be suitable for sample determination. Uric acid was separated and detected within 2.3 min, with a linear response range from 5 to 400 µg mL −1 and a correlation coefficient of 0.9998. Intraday and interday precision were 1.6 and 2.5 % ( n = 6), respectively. The recoveries were between 98.3 and 100.5 %. This simple, effective, and stable method is a good alternative to existing methods for uric acid determination. Also, the improved electromagnetic induction detector holds great promise in clinical analysis.
研究领域:
分析化学
·
临床分析
·
电泳技术
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Huifang Zhang,Hui Xu
In this article, silver nanoparticles (AgNPs) modified nanofibers that represent a novel kind of packing materials for micro-solid phase extraction (μ-SPE) is reported for the first time. The composite material was fabricated through in-situ formation of AgNPs on the polydopamine (PDA) coated polystyrene electrospun fibers (PS@PDA-Ag). The nanofibers displayed desirable hydrophilicity, large surface area, high porosity, good extraction ability and certain capacity to resist matrix interference. A μ-SPE combined with liquid chromatography-mass spectrometry (LC–MS) method was developed for the online determination of three monohydroxy polycyclic aromatic hydrocarbons (OH-PAHs) metabolites in human urine. Under the optimal conditions, good linearity (0.02–5 ng mL −1 ) was acquired with the correlation coefficients (R 2 ) being larger than 0.9962. Low limits of detection (0.007–0.032 ng mL −1 ) expressed the satisfactory sensitivity of the method. Moreover, the intraday relative standard deviation values lower than 9.7% and the recoveries among the range of 71–116% were obtained. In general, the online μ-SPE-LC–MS method had the features of simplicity, rapidity, sensitivity and automation, and it was expected to become a promising approach for the online analysis of trace OH-PAHs in complex biological samples.
研究领域:
分析化学
·
环境科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Lei Xu,Jing Lu,Weiqi Meng,Fengjuan Cui
Micellar electrokinetic chromatography (MEKC) with ultraviolet detection was employed for the simultaneous determination of the nucleoside reverse transcriptase inhibitors lamivudine, stavudine and didanosine with the ionic liquid-based cationic surfactant 1-tetradecyl-3-methylimidazolium bromide ([C14MIm]Br) as the pseudostationary phase. The composition, pH, and concentration of running buffer, surfactant concentration, separation voltage, and injection time were optimized. In comparison with conventional cationic surfactants, improved separation was observed using [C14MIm]Br. The applicability of the method was demonstrated by the analysis of human urine. The method exhibited wide linear ranges with limits of quantitation of 0.5, 0.3, and 1.0 μg/mL for lamivudine, stavudine, and didanosine, respectively. The intra-day and inter-day accuracies were from 91.5% to 114.0%. The precision, expressed as relative standard deviation (RSD), was less than 12.6% for the peak area. The recoveries of the analytes in the samples were between 91.8% and 97.3%. This approach is convenient, rapid, and economical for these analytes in biological samples.
研究领域:
分析化学
·
医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Wenli Zhu,Jing Zhang,Xiaowan Zhang,Lizhen Han,Peige Qin,Shufang Tian,Qian Zhou,Xuebin Zhang,Minghua Lu
The Al-doped mesoporous crystalline material-41 (Al-MCM-41) composite was prepared and applied as fiber coating material of headspace solid-phase microextraction (HS-SPME) for extraction of polycyclic aromatic hydrocarbons (PAHs) from human urine. Five PAHs including acenaphthene, fluorene, phenanthrene, anthracene, and pyrene are chosen as target analytes to evaluate the performance of the material by GC-FID analysis. The mesoporous Al-MCM-41 composite was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption measurement, and thermogravimetric analysis. The parameters affecting the extraction efficiency of HS-SPME were investigated. Under the optimal conditions, the method exhibits ideal linearity for target analytes in the range of 0.3–600 ng⋅mL -1 with the coefficients (R 2 ) equal or higher than 0.9906. The enrichment factors are calculated from 540 to 1760. The limits of detection (LODs) and limits of quantitation (LOQs) are between the ranges of 0.06–0.18 and 0.3–0.9 ng⋅mL -1 , respectively. The relative standard deviations (RSDs) ( n = 5) of intra-day and inter-day are in the ranges of 1.08–7.49% and 2.84–18.3% respectively. The fiber-to-fiber reproducibility ( n = 3) is in the range of 6.47–13.9%. The method was successfully applied for the analysis of PAHs in human urine with reasonable recoveries which is ranging from 73.29 to 116.1%.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2014
·
IF 2.829
Yajing Lei,Xiaoyi Li,Muhammad Sajid Hamid Akash,Lifang Zhou,Xiaojing Tang,Weixing Shi,Zhiming Liu,Shuqing Chen
Salbutamol (SAL) is known as quick-acting β-2 receptor agonist and its use in humans for pulmonary diseases and/or in animal feed is limited because of associated potential hazardous effects on health. Several techniques are available for the detection of SAL, but are expensive and time consuming. Here for the first time, a novel pre-assembled DNA immunoprobe-based immuno-PCR assay was developed to investigate the levels of SAL in human urine samples and compared the proposed immuno-PCR assay for the detection of SAL with that of direct competitive ELISA. Under the optimized conditions, the assay showed a linear range over seven orders of magnitude, whereas the limit of detection of SAL in buffer was found to be 21 fg/mL. Current immuno-PCR assay exhibited a 300-fold better detection limit for SAL as compared to one achieved with direct competitive ELISA using the same antibody. In conclusion, it has been found that the developed method is highly sensitive and simple method that can significantly enhance the detection sensitivity for small molecules. Moreover it can be modified and used for detecting other small molecules and/or chemicals that exist in the environment and are responsible for the environmental pollution.
研究领域:
分析化学
·
分子生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2016
·Vol. 32
·期 1
·4-10
·
IF 1.452
Dan Liu,Jingdong Peng,Shaopu Liu,Mingqiong Zhou,Jing Zhang,Aiping Li
A highly selective and sensitive method of reversed phase high-performance liquid chromatography (RP-HPLC) coupled with resonance Rayleigh scattering (RRS) was developed for the determination of procaine, bupivacaine and tetracaine. Separation of three local anaesthetics was achieved at 35 °C on a C 18 column. The mobile phase was 30: 70 (v/v) acetonitrile/triethylamine–phosphoric acid buffer (pH 2.9) at flow rate of 0.3 mL/min. The RRS detection was conducted by taking advantage of the strong RRS enhancement of the local anaesthetics with erythrosine reaction in an acidic medium. Under optimum conditions, the limit of detection (S/N = 3) values were in the range of 2.4–11.2 ng/mL. Recoveries from spiked human urine samples were 95.8%–104.5%. The proposed method applied to the determination of local anaesthetics in human urine achieved satisfactory results. In addition, the mechanism of the reaction is fully discussed. Copyright © 2016 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESOURCES CONSERVATION AND RECYCLING
·
2022
·
IF 13.716
Qing Jiang,Jia Liu,Xiangru Song,Ye Qiu,Jianliang Xue,Yuqiang Shao,Yujie Feng
Human urine contains abundant nutrients, while separately collecting urine can significantly alleviate nutrient loads in existing wastewater treatment facilities. Herein,0020a novel forward osmosis integrated bioelectro-concentration and recovery system (OsBCRS) was developed to recover nutrient ions from urine. NH 4 + and PO 4 3– migrated out from urine and enriched in the internal chamber of OsBCRS driven by the self-generated electric field, which were further concentrated by water extraction from the integrated forward osmosis (FO) process, and finally recovered as struvite. The current density of OsBCRS reached 27.3 A m –3 , which in turn drove the migration of NH 4 + -N (93.7 ± 1.6%) and PO 4 3– -P (94.6 ± 0.8%) out from urine, and these separated nutrients were enriched by 9.4–9.5 times in the internal chambers. Subsequently, these enriched nutrients in the catholyte were 3.2-fold concentrated with 68.6% of volume reduction by FO. The system removed up to 83.4% of NH 4 + -N and 88.8% of PO 4 3– -P, most of which were recovered as struvite taking advantage of reverse-fluxed Mg 2+ . The recovered struvite exhibited similar crystal structure and composition to that of standard struvite. The preliminary operating cost analysis of OsBCRS demonstrated that the revenue, including electricity generation and struvite recovery, could offset the operating cost. This study demonstrated highly efficient enrichment and recovery of nutrient ions from human urine by OsBCRS in a cost-effective way, and would encourage further exploration for nutrients recovery from wastes.
研究领域:
环境工程
·
废水处理
·
资源回收
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）