INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
·
2014
·
IF 1.487
Lu Zhang,Xiaoyu Liu,Jin Qiu,Xin Yao,Peiyuan Zhou,Mengqi Li
A molecularly imprinted polymer was prepared using diethylphosphonoacetic acid (DPA) as a dummy template for use as an artificial receptor for organophosphrous pesticides (OPPs). Scanning electron microscopy (SEM) was employed to observe the morphology of the prepared polymer. Ultraviolet spectroscopy and 1H NMR analysis reveal that DPA and the monomer integrate by hydrogen bonding with a molar ratio of 1:2. A static adsorption experiment, Scatchard analysis, and dynamic adsorption and selective binding tests demonstrated that the obtained polymer can effectively bind a group of OPPs containing dimethoxyl or diethyoxyl.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qianjin Li,Rui Li,Mengdi Wang,Jinjin Wang,Tingting Wang,Yu Jin,Jianlin Li,Fenying Wang
Development of rapid, low-cost, and sensitive sensors for monitoring Escherichia coli (E. coli) O157:H7 is very necessary and urgent to ensure food safety. In this study, we developed two fluorescent artificial receptor-based sensors via a boronate affinity molecular imprinting strategy targeting E. coli O157:H7. Fluorescein- and rhodamine-grafted artificial receptors were synthesized using bacterial surface glycoproteins and lipopolysaccharides as the molecular-sized templates to create the specific imprinted cavities. The fluorescent sensors can achieve the recognition within 5 min, displaying cross-reactivity factors of 2.6–40.6 (fluorescein) and 1.5–18.4 (rhodamine), and ultra-sensitive detection limits of 7 CFU/mL (fluorescein) and 2 CFU/mL (rhodamine). The fluorescent artificial receptor-based sensors showed spiking recovery rates in the range of 81 %∼101 %, exhibiting high accuracy as the classical plate counting method (71 %∼112 %) in milk and tap water samples. This strategy establishes a universal platform for constructing high-performance fluorescent sensors for rapidly screening pathogenic bacteria in food safety.
研究领域:
生物传感器
·
食品微生物学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Tingting Wang,Qianjin Li,Meng Wang,Jianhong Xu,Jianlin Li,Fenying Wang
Development of multiple detection methods to monitor non-steroidal anti-inflammatory drugs (NSAIDs) in food is an effective way to protect human health. Here, we aimed to synthesize fluorescent artificial receptors by molecular imprinting technique to construct a simultaneous detection system targeting NSAIDs. Rhodamine B and fluorescein-functionalized silanes were employed as the fluorescence signal reporters for naproxen and ketoprofen, respectively. Two fluorescent molecularly imprinted polymers (FMIPs) were obtained with high specificity, giving cross-reactivity factors of 6.4–15.8 (naproxen) and 2.6–25.6 (ketoprofen). Both FMIPs also displayed rapid response time (5 min) and high sensitivity (detection limit at ∼ nM level). A simultaneous detection system was constructed based on the FMIPs and applied for sensing the spiked NSAIDs in real samples, showing recoveries of 71–119 %, comparable with the HPLC methods (70–113 %). In summary, use of different FMIPs to construct simultaneous detection systems is practicable, and provides a flexible way for sensing multiple hazards in food samples.
研究领域:
分析化学
·
食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2019
·Vol. 7
·期 27
·16161-16175
·
IF 10.733
Jinxin Liu,Pan Wang,Mengdie Zhou,Yue Ma,Xiangheng Niu,Guoqing Pan,Jianming Pan
Potentially toxic dichlorophenols and heavy metal ions commonly coexist in industrial sewage, posing a serious threat to public health and the environment and making the treatment more challenging. Inspired by Suzhou bifacial embroidery, whereby the Janus-like planar structure allows different compositions to be compartmentalized onto two distinct surfaces, Janus silica nanosheets partitioning bispecific artificial receptors (i.e., molecularly imprinted sites and thiol groups) onto distinct recognition surfaces (J-MIPs/cys) were designed to independently take up 2,6-dichlorophenol (DCP) and Pb(II). For J-MIPs/cys, the thiol groups are expected to capture Pb(II) via chelating action and molecularly imprinted cavities act as highly specific recognition sites for DCP. J-MIPs/cys possessed uniform cross-sectional dimensions and thickness (∼25.3 nm), and a particle-like surface imprinted layer (∼21.8 nm). In the monocomponent system, J-MIPs/cys displayed fast binding kinetics (60 min and 45 min), large monolayer adsorption capacity (129.4 mg g−1 and 82.95 mg g−1), high affinity, and excellent regeneration after five reuse cycles (9.134% and 12.16% loss in adsorption amounts) for DCP and Pb(II), respectively. Additionally, a spontaneous and endothermic adsorption process was also confirmed. In a binary system, the presence of Pb(II) produced a prominent increase in DCP adsorption at high initial concentrations and a decrease at low initial concentrations, owing to the partial complexation of Pb(II) with excess DCP and occupation of imprinted sites by Pb(II). However, the uptake of Pb(II) was inhibited over the whole range of concentration in the presence of DCP due to the competitive adsorption onto thiol groups. In summary, J-MIPs/cys integrating novel bispecific receptors open wide possibilities for efficient and specific treatment of coexisting toxic pollutants.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Dandan Yao,Lei Zhang,Jiaojiao Huang,Chenghong Sun,Yu Zhang,Xiaoli Gu,Chong-Zhi Wang,Fei Li,Lina Chen,Chun-Su Yuan
In this work, surface magnetic molecularly imprinted polymers (SMMIPs) were synthesized and used as artificial receptors in the dispersive magnetic solid phase extraction (DMSPE) for capturing potential neuronal nitric oxide synthase–post synaptic density protein-95 (nNOS–PSD-95) uncouplers, which is known as neuroprotection against stroke. Factors that affected selective separation and adsorption of the artificial receptors, such as the amount of template, the types of functional monomer and porogen solvents, and the molar ratio of template/functional monomer/cross-linker were optimized. The artificial receptors were also characterized using fourier transformed infrared, scanning electron microscope, thermal gravimetric analysis and physical property measurement systems. Multiple interactions between template and SMMIPs led to larger binding capacities, faster binding kinetics, quicker separation abilities and more efficient selectivity than the surface magnetic nonimprinted polymers (SMNIPs). The SMMIPs were successfully applied to capture potential nNOS–PSD-95 uncouplers from complex samples, and eight compounds were seized and confirmed rapidly when combined with HPLC and MS. The detection of the new nNOS–PSD-95 uncouplers ranged from 0.001 to 1.500 mg/mL with correlation coefficients of 0.9990–0.9995. The LOD and LOQ were 0.10–0.68 μg/mL and 0.47–2.11 μg/mL, respectively. The neuroprotective effect and co-immunoprecipitation test in vitro revealed that Emodin-1- O -β- d -glucoside, Rhaponticin, Gnetol and 2,3,5,4′-Tetrahydroxystilbene-2- O -β- d -glucoside have neuroprotective and uncoupling activities, and that they may be the new uncouplers of nNOS–PSD-95
研究领域:
药理学
·
分析化学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Advances
·
2023
·Vol. 9
·期 9
·
IF 13.6
He Li,Yue Yan,Jing Chen,Ke Shi,Chuwen Song,Yanglimin Ji,Liyan Jia,Jianming Li,Yan Qiao,Yiyang Lin
Engineering artificial cellular systems capable of perceiving and transmitting external signals across membranes to activate downstream targets and coordinate protocellular responses is key to build cell-cell communications and protolife. Here, we report a synthetic photoreceptor–mediated signaling pathway with the integration of light harvesting, photo-to-chemical energy conversion, signal transmission, and amplification in synthetic cells, which ultimately resulted in protocell subcompartmentalization. Key to our design is a ruthenium-bipyridine complex that acts as a membrane-anchored photoreceptor to convert visible light into chemical information and transduce signals across the lipid membrane via flip-flop motion. By coupling receptor-mediated phototransduction with biological recognition and enzymatic cascade reactions, we further develop protocell signaling–encoded Boolean logic gates. Our results illustrate a minimal cell model to mimic the photoreceptor cells that can transduce the energy of light into intracellular responses and pave the way to modular control over the flow of information for complex metabolic and signaling pathways.
研究领域:
合成生物学
·
生物物理学
·
细胞工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Mingming Zhang,Juan Qiao,Li Qi
An artificial receptor approach for proteins capture and release was designed based on ionic-sensitive and thermo-sensitive functionalized polymers, which were modified onto the surface of magnetic nanoparticles . The dual-functional poly(styrene-sulfonate- N -isopropylacrylamide) (P(SS-NIPAm)) was synthesized via the free radical polymerization method and used as the artificial receptor with electrostatic, hydrophilic and hydrophobic properties. The affinity between the artificial receptor and lysozyme was investigated using high performance liquid chromatography . Arising from the strong and specific interactions between the negatively charged sulfonic acid groups of P(SS-NIPAm) and positively charged amino groups of the protein, the prepared artificial receptor exhibited outstanding affinity for lysozyme. Interestingly, due to the thermo-responsive and ionic strength-responsive features of the prepared P(SS-NIPAm), the capture and release process for lysozyme could be modulated by varying the environmental temperature and ionic strength . Further, the proposed artificial receptor based on P(SS-NIPAm) modification of magnetic nanoparticles surface was used for affinity capture of lysozyme in human serum with a recovery ranging from 89.9% to 100.4%, paving a new way for synthesis of artificial receptors and showing great potential for recognition of target protein in real bio-samples.
研究领域:
纳米技术
·
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）