JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 6
·2321-2329
·
IF 3.639
Jian Gao,Meng M Li,Guangyong Zhao
BACKGROUND Nitrous oxide (N 2 O) is a potent greenhouse gas depleting the stratospheric ozone. Previous studies reported that the thiocyanate (TC) excretion in the urine of cattle fed rapeseed meals containing glucosinolates was positively correlated with the N 2 O-nitrogen (N) emissions. The objectives of the experiment were to verify the effects and the mechanism of TC on the N 2 O-N emissions from the soil applied with artificial urine using static incubation technique. Four levels of TC, that is 0.00, 0.26, 0.78 and 2.33 mmol L −1 were composited in artificial urine as experimental treatments. Soil inorganic N and bacterial community were analyzed to elucidate the effects of TC on the N 2 O-N emissions of artificial urine. RESULTS Adding TC increased the N 2 O-N fluxes, the N 2 O-N to N application ratio, and the estimated N 2 O-N emissions from the soil applied with artificial urine both linearly and quadratically. The estimated N 2 O-N emission ( Y , in μmol) was increased with the TC adding level ( X , in μmol) in a quadratic manner: Y = 52.57 + 4.47 X − 0.123 X 2 ( R 2 = 0.70). Adding TC did not affect the soil bacterial diversity and richness, but increased the relative abundances of Nitrosomonas (both for nitrification and denitrification) and Hyphomicrobium , Lysobacter and Terrimonas (for denitrification), and tended to increase the relative abundances of denitrification and dissimilatory nitrate reduction to ammonium. CONCLUSION TC increased the N 2 O-N emissions of the soil applied with artificial urine possibly through enhancing the denitrification of nitrifiers in the soil. Field experiments are necessary to verify the laboratory results. © 2021 Society of Chemical Industry.
研究领域:
环境科学
·
微生物学
·
土壤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Jialu Shen,Guoqing Chen,Zichen Yang,Yamin Wu,Chaoqun Ma,Lei Li,Taiqun Yang,Jiao Gu,Hui Gao,Chun Zhu
The accurate detection of fructose in human urine can help prevent and screen for diseases such as fructokinase deficiency and hereditary fructose intolerance. Surface-enhanced Raman spectroscopy (SERS) is an analytical technique with selectivity and high sensitivity, which has been widely applied to the detection of targets with complex backgrounds. In this work, 4-mercaptophenylboronic acid (4-MPBA) was modified on the surface of silver nanoparticles (AgNPs) under mild conditions to obtain a boronic acid-functionalized SERS substrate for the detection of fructose in artificial urine. The detection mechanism was based on the deboronization reaction of 4-MPBA on the surface of AgNPs, which was induced by fructose, and the Raman signal of the generated thiophenol (TP) molecules was significantly enhanced by the silver nanoparticles, with a linear increase in SERS peak intensity at 1570 cm −1 . We achieved the detection limits of 0.084 µmol/L in water and 0.535 µmol/L in urine by this method. The relative standard deviation (RSD) in the recovery experiments of urine ranged from 1.01 % to 2.22 %, and the whole detection time was less than 10 min, which indicated that this method is highly reliable for fructose detection and has a good prospect in bioassay and clinical medicine.
研究领域:
分析化学
·
生物医学工程
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Jianlu Zheng,Wencui Zhang,Yanli Gong,Wenlang Liang,Yongxiang Leng
Lithocholic acid (LCA), a secondary bile acid, has emerged as a potential early diagnostic biomarker for various liver diseases. In this study, we introduce a novel near-infrared (NIR) polymethine dye-based biosensor, capable of sensitive and selective detection of LCA in phosphate buffer and artificial urine (AU) solutions. The detection mechanism relies on the formation of J-aggregates resulting from the interplay of 3,3-Diethylthiatricarbocyanine iodide (DiSC 2 (7)) dye molecules and LCA, which induces a distinctive red shift in both absorption and fluorescence spectra. The biosensor demonstrates a detection limit for LCA of 70 μM in PBS solution (pH 7.4), while in AU solution, it responds to an LCA concentration as low as ∼60 μM. Notably, the proposed biosensor exhibits outstanding selectivity for LCA, effectively distinguishing it from common interferents such as uric acid, ascorbic acid, and glucose. This rapid, straightforward, and cost-effective spectrometer-based method underscores its potential for early diagnosis of liver diseases by monitoring LCA concentrations.
研究领域:
分析化学
·
生物医学工程
·
医疗诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Hao Gu,Jiwen Li,Qiong Liang,Wei Xu
Modern solid phase microextraction (SPME) device linked with mass spectrometry (SPME-MS) has evolved from producing ion chronogram as flat noisy signal to as unimodal-like signal. We designed a SPME device, which is closer in morphology to LC column, linked it with a miniature mass spectrometer (SPME-Mini MS), and proposed a mathematical model that elution of compound from the SPME device is equivalent to overlay of elution of the compound from the infinite LC columns with the lengths between 0 and the length of the device and it can generate an ion chronogram as right-skew unimodal signal. Rhodamine B as analyte was used for experimental verification and its unimodal signal was used to fit the parameters of a computer simulation program based on the model. The experimental results and simulations empirically cross-confirmed that SPME-Mini MS can generate ion chronogram as clean right-skew unimodal signal. Furthermore, the SPME-Mini MS system was used for quantitative analysis of psychotropic drugs (i.e. risperidone and aripiprazole) in artificial urine. The results preliminarily demonstrated that the system can utilize area under unimodal signal for quantitative analysis and has potential to be applied for on-site, fast and accurate quantification of drugs and other compounds.
研究领域:
分析化学
·
质谱分析
·
药物分析
·
微萃取技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2019
·
IF 8.063
Huijuan Yu,Wen-Chao Geng,Zhe Zheng,Jie Gao,Dong-Sheng Guo,Yuefei Wang
Detection and quantification of trimethylamine N -oxide (TMAO), a metabolite from gut microbial, is important for the disease diagnosis such as atherosclerosis, thrombosis and colorectal cancer. In this study, a novel method was established for the sensing and quantitative detection of TMAO via molecular recognition of guanidinium-modified calixarene from complex matrix. Methods : Various macrocycles were tested for their abilities to serve as an artificial TMAO receptor. Using the optimized receptor, we developed an indicator displacement assay (IDA) for the facile fluorescence detection of TMAO. The quantification of TMAO was accomplished by the established calibration line after excluding the interference from the various interfering substances in artificial urine. Results : Among various macrocycles, water-soluble guanidinium-modified calix[5]arene (GC5A), which binds TMAO in submicromolar-level, was identified as the optimal artificial receptor for TMAO. With the aid of the GC5A•Fl (fluorescein) reporter pair, TMAO fluorescence “switch-on” sensing was achieved by IDA. The fluorescence intensity increased linearly with the elevated TMAO concentration. The detection was not significantly interfered by the various interfering substances. TMAO concentration in artificial urine was quantified using a calibration line with a detection limit of 28.88 ± 1.59 µM, within the biologically relevant low µM range. Furthermore, the GC5A•Fl reporter pair was successfully applied in analyzing human urine samples, by which a significant difference in fluorescence response was observed between the [normal + TMAO] and normal group. Conclusion: The proposed supramolecular approach provides a facile, low-cost and sensitive method for TMAO detection, which shows promise for tracking TMAO excretion in urine and studying chronic disease progression in humans.
研究领域:
分析化学
·
生物医学工程
·
分子生物学
·
临床化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
He Cheng,Langlang Yi,Jianduo Wu,Guoqian Li,Gang Zhao,Zhixiang Xiao,Bo Hu,Lei Zhao,Jie Tian
Drug detection in biofluids has always been great importance for clinical diagnosis. Many detection technologies such as chromatography-mass spectrometry, have been applied to the detection of drugs. However, these technologies required multi-step operations, including complicated and time-consuming pretreatment processes and operations of bulky detection instruments, significantly limiting development of drug detection in clinical diagnosis. Herein, a portable 3D-printed paper cartridge was fabricated for fast sample preconcentration and direct drugs quantitative detection in biofluids by a portable Raman spectrometer. This cartridge contained both paper tip with silver nanowires to preconcentrate samples and achieve surface-enhanced Raman Scattering (SERS) measurement, and 3D-printed cartridge to build enclosed environment for the improvement of detection, which cost only one dollar. The preconcentration ability of the cartridge was up to 18.13-fold fluorescence enhancement and compared to the non-preconcentration method, it achieved 9.93-fold improvement of SERS performance. The anticancer drug of epirubicin hydrochloride, cyclophosphamide and their mixtures were quantitatively detected in the bovine serum or artificial urine. The integrated detection procedure required only 1 h, including sample pretreatment and preconcentration, drying, SERS measurements, and quantification analysis. This 3D-printed paper cartridge constituted a portable detection platform that would be potentially a practical and point-of-care detection tool for drug preconcentration and quantification on the clinical diagnosis.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Xinyue Mao,Wenting Cao,Xinyan Yuan,Xiao Liang,Guanghui Zhang,Yongzhong Wang,Xiaofeng Jin
Acute kidney injury (AKI) is a global health problem due to its high rates of morbidity and mortality. Kidney injury molecule-1 (KIM-1) is considered as a potential biomarker for the diagnosis of AKI. In this study, we proposed an oriented antibody immobilization strategy for reliable detection of KIM-1 by utilizing single-stranded DNA with a methylene blue tag (MB-ssDNA) to achieve directional immobilization of antibodies onto poly methylene blue (PMB) on the electrode surfaces through cyclic voltammetry (CV). PMB not only enhanced electron transfer rate but also provided a platform for the oriented immobilization of antibody. The proposed immunosensor demonstrated a limit of detection (LOD) of 5.8 pg/mL with a linear range from 1 pg/mL to 100 pg/mL. The immunosensor exhibited good repeatability and reusability as well as excellent operational stability. Furthermore, we successfully developed a low-cost and portable paper-based microfluidic electrochemical device to measure KIM-1 concentrations in artificial urine. Integrated flexible electrochemical immunosensors based on antibody orientation immobilization and electrochemical signal amplification have great application potential in personalized point-of-care (POC) clinical diagnosis.
研究领域:
生物医学工程
·
分析化学
·
即时诊断
·
电化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Pengfei Li,Ting Wang,Jing He,Jianxin Jiang,Fuhou Lei
Three novel polyelectrolyte galactomannan hydrogels (PGHs) were fabricated by chemically crosslinking quaternary ammonium galactomannan (QAG) and carboxymethyl galactomannan (CMG), and employed for the removal of Congo Red (CR) and Methylene Blue (MB). Physicochemical characterization revealed that the PGHs are chemically and physically crosslinked. The PGHs are pH- and ion-sensitive, and their physical crosslinking can be destroyed by artificial urine; water swelling capacity (100.6–321.9 g/g dry gel) and artificial urine swelling capacity (35.9–80.5 g/g dry gel). The adsorption of CR and MB was studied and found to be pH-dependent and selective. The maximum adsorption capacities of CR and MB on the QAG and CMG gels are 1441 and 94.52 mg/g, respectively, and their adsorption kinetics and isotherm behavior obey the pseudo-second-order kinetics model and Langmuir isotherm model, respectively. The adsorption mechanism is dominated by electrostatic interactions and hydrogen bonding. Further, the PGHs have excellent salt resistance and are reusable.
研究领域:
材料科学
·
环境科学
·
化学工程
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）