AFRICAN JOURNAL OF BIOTECHNOLOGY
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2011
·Vol. 8
·期 24
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IF 0.573
L Chun,Y Xiaoping,H Guangrong,W Hong
A rapid, simple and low cost procedure for preparing hapten-protein conjugates was developed using gramicidin A (GA) and two other water-soluble proteins, keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). GA was a kind of antimicrobial peptides. Two lysines and a cysteine were linked to amino- terminus and carboxyl-terminus of the peptide chain, respectively, in order to form sulfhydryl groups and improve its water solubility. And the molecule weight and purity of the modified peptidechain were checked by electron spray ionization mass spectrometry (ESI-MS) and high performance liquid chromatography (HPLC). Then the activated KLH and OVA were conjugated to GA by using 4-(Nmaleimidomethyl) cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt (sulfo-SMCC) to form disulfide linkage. In order to obtain artificial antigen of GA, GA was linked to KLHand OVA by sulfo-SMCC coupling at room temperature. The conjugates of KLH-Peptide and OVAPeptide were identified by infrared and ultraviolet spectrophotometry SDS-PAGE and DTNB method, which could prove the activity of the sulfhydryl-groups. In this way, we have obtained a novel artificial immunogen and coating antigen that could be used to raise polyclonal antibody through immunized rabbits.
研究领域:
免疫学
·
生物化学
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DOI
高影响因子
Small
·
2024
·
IF 13
Lingkai Dong,Minchao Liu,Meng Fang,Qianqian Lu,Xingjin Li,Yanming Ma,Tiancong Zhao
Albeit microemulsion systems have emerged as efficient platforms for fabricating tunable nano/microstructures, lack of understanding on the emulsion-interfacial assembly hindered the control of fabrication. Herein, a nucleation-inhibited microemulsion interfacial assembly method is proposed, which deviates from conventional interfacial nucleation approaches, for the synthesis of polydopamine microvesicles (PDA MVs). These PDA MVs exhibit an approximate diameter of 1 µm, showcasing a pliable structure reminiscent of cellular morphology. Through modifications of antibodies on the surface of PDA MVs, their capacity as artificial antigen presentation cells is evaluated. In comparison to solid nanoparticles, PDA MVs with cell-like structures show enhanced T-cell activation, resulting in a 1.5-fold increase in CD25 expression after 1 day and a threefold surge in PD-1 positivity after 7 days. In summary, the research elucidates the influence of nucleation and interfacial assembly in microemulsion polymerization systems, providing a direct synthesis method for MVs and substantiating their effectiveness as artificial antigen-presenting cells.
研究领域:
生物材料学
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免疫学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Chao Xing,Xiaoqiu Dou,Laiben Gao,Peng Xu,Yinbo Peng,Kaikai Yang,Yong Fang,Kai Hou,Shegan Gao,Chuanliang Feng
Biomimetic artificial antigen-presenting cells (aAPCs) have emerged as a promising platform for the immune system activation and modulation. However, abundant serum proteins in the living body prefer to adsorb on the aAPC surface to form a protein corona, leading to rapid clearance of aAPCs by mononuclear phagocytes, causing inefficient tumor immunotherapy by aAPCs. To achieve long-lasting antitumor immunity, chiral aAPCs with left-handed (aAPCs-L) or right-handed (aAPCs-D) helices are constructed via coassembly, chiral induction, chiral memory, and a click reaction. aAPCs-L can efficiently prevent the formation of a protein corona since the left-handed helix of aAPCs-L and the right-handed helix of serum proteins are structurally mismatched. Compared with aAPCs-D and achiral aAPCs (aAPCs-R), aAPCs-L can extend the blood circulation half-life from 4.15–4.72 h to more than 24 h via evading macrophage uptake and clearance, performing sustained activation of antitumor immune response. In vivo treatment with aAPCs-L results in invisible residual melanoma on day 25 (but 947.3 ± 122.8 mm2 residual melanoma for aAPCs-R and 656.1 ± 135.0 mm2 residual melanoma for aAPCs-D). This study demonstrates that incorporating the chiral structure into the design of aAPCs improves pharmacokinetics and enhances the efficacy of tumor immunotherapy, which provides useful insights into the development of advanced biomedical materials for cancer treatment.
研究领域:
免疫学
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生物工程
·
纳米医学
·
癌症生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jianlin Li,Tingting Cai,Wei Li,Weiwei Li,Laicui Song,Qianjin Li,Guangping Lv,Jialong Sun,Saisai Jiao,Siwei Wang,Yu Jin,Tiesong Zheng
A new protein microarray method for multiplex mycotoxin detection in parallel has been established on a stable TiO2-modified porous silicon (PSi) surface. A typical competitive immunoassay microarray protocol has been developed for simultaneous detection of multiplex mycotoxins including aflatoxin B1 (AFB1), ochratoxin A (OTA), and fumonisin B1 (FB1) on the TiO2-PSi surface. The epoxy groups were selected to modify the surface of a TiO2-PSi wafer for the immobilization of artificial antigens of mycotoxins because of their high signal-to-noise ratios. Under optimal conditions, the developed method showed wide linear detection ranges of 0.01–1 ng/mL for OTA, 0.001–1 ng/mL for AFB1, and 0.01–1 ng/mL for FB1 and low limit of detections (LODs) of 0.433 ng/mL for OTA, 0.243 ng/mL for AFB1, and 0.093 ng/mL for FB1. The microarray method can specifically identify the three mycotoxins and their analogues. The recovery rates in real samples were within 75–120%, which were in agreement with that of the classical ELISA method. The new method has great application potential for rapid, sensitive, and high-throughput screening of multiplex mycotoxins and other target molecules.
研究领域:
分析化学
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毒理学
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食品安全
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Xin Shou,Yunru Yu,Dan Wu,Fang Wang,Weijian Sun,Ping Duan,Luoran Shang
Adoptive T cell immunotherapy is a powerful strategy for clinical cancer treatment. As a critical step to generate clinical-grade T cell products for therapeutic applications, novel approaches for T cell activation and expansion attract increasing attention. Here, we develop hydrogel-integrated natural pollens as artificial antigen-presenting scaffolds for ex vivo T cell expansion. Black phosphorus is presented in the scaffolds that allow for NIR-triggered release of cytokines and antibodies for T cell activation. Besides, the distinctive spike structure and high surface area of pollens contribute to T cell cluster formation and enhance their local proliferation. After ex vivo expansion, the activated T cells exhibit robust antitumor activity. Therefore, these features demonstrate that the novel pollen-based antigen-presenting scaffolds are promising for therapeutic T cell preparation.
研究领域:
免疫学
·
癌症治疗
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）