Nontarget analysis has gained prominence in screening novel perfluoroalkyl and polyfluoroalkyl substances (PFASs) in the environment, yet remaining limited in human biological matrices. In this study, 155 whole blood samples were collected from the general population in Shijiazhuang City, China. By nontarget analysis, 31 legacy and novel PFASs were assigned with the confidence level of 3 or above. For the first time, 11 PFASs were identified in human blood, including C1 and C3 perfluoroalkyl sulfonic acids (PFSAs), C4 ether PFSA, C8 ether perfluoroalkyl carboxylic acid (ether PFCA), C4–5 unsaturated perfluoroalkyl alcohols, C9–10 carboxylic acid-perfluoroalkyl sulfonamides (CA-PFSMs), and C1 perfluoroalkyl sulfonamide. It is surprising that the targeted PFASs were the highest in the suburban population which was impacted by industrial emission, while the novel PFASs identified by nontarget analysis, such as C1 PFSA and C9–11 CA-PFSMs, were the highest in the rural population who often drank contaminated groundwater. Combining the toxicity prediction results of the bioaccumulation potential, lethality to rats, and binding affinity to target proteins, C3 PFSA, C4 and C7 ether PFSAs, and C9–11 CA-PFSMs exhibit great health risks. These findings emphasize the necessity of broadening nontarget analysis in assessing the PFAS exposure risks, particularly in rural populations.
In this study, a novel approach is adapted for the synthesis of new nanostructures of cobalt oxide (Co 3 O 4 ) by low temperature aqueous chemical growth method in the presence of water soluble amino acids such as L-arginine, L-aspartic acid, L-isoleucine, L-leucine, L-serine and L-threonine. The synthesized nanostructures were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and high resolution transmission electron microscopy (HRTEM). The nanostructures exhibit well defined morphology and are of high purity. To explore the potential application of the prepared nanostructures of cobalt oxide, the non-enzymatic glucose sensor is developed by modifying the glassy carbon electrode. Serine assisted cobalt oxide nanostructures showed excellent sensitivity of 4169.00 μA/mM cm 2 , wide linear range of 0.01 mM to 20 mM and detection limit was estimated as 0.001 mM (S/N=3). The analytical parameters for the serine assisted cobalt oxide nanostructures based glucose sensor were evaluated and it was found that the sensor is highly selective, reproducible, repeatable, reversible, and stable. Finally, the practical application of the serine assisted cobalt oxide nanostructures based glucose sensor was examined by measuring the glucose concentrations from real human blood samples.
危险品化学品经营许可证（带存储）