Bioactive Materials
·
2023
·
IF 18.9
Huijuan Wang,Deshun Sun,Weiming Lin,Chao Fang,Kui Cheng,Zhengzhou Pan,Daping Wang,Zhangfa Song,Xiaojun Long
Full-thickness skin wounds are have continued to be reconstructive challenges in dermal and skin appendage regeneration, and skin substitutes are promising tools for addressing these reconstructive procedures. Herein, the one-step fabrication of a cell sheet integrated with a biomimetic hydrogel as a tissue engineered skin for skin wound healing generated in one step is introduced. Briefly, cell sheets with rich extracellular matrix, high cell density, and good cell connections were integrated with biomimetic hydrogel to fabricate gel + human skin fibroblasts (HSFs) sheets and gel + human umbilical vein endothelial cells (HUVECs) sheets in one step for assembly as a cell sheet-laden hydrogel (CSH). The designed biomimetic hydrogel formed with UV crosslinking and ionic crosslinking exhibited unique properties due to the photo-generated aldehyde groups, which were suitable for integrating into the cell sheet, and ionic crosslinking reduced the adhesive force toward the substrate. These properties allowed the gel + cell sheet film to be easily released from the substrate. The cells in the harvested cell sheet maintained excellent viability, proliferation, and definite migration abilities inside the hydrogel. Moreover, the CSH was implanted into a full-thickness skin defects to construct a required dermal matrix and cell microenvironment. The wound closure rate reached 60.00 ± 6.26% on the 2nd day, accelerating mature granulation and dermis formation with skin appendages after 14 days. This project can provide distinct guidance and strategies for the complete repair and regeneration of full-thickness skin defects, and provides a material with great potential for tissue regeneration in clinical applications.
研究领域:
组织工程学
·
再生医学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Emerging Contaminants
·
2024
·
IF 5.3
Chao Zhu,Mingyue Pang,Wenwen Gong,Hengming Pang,Han Qu
Increasing numbers of chiral insecticides have been released into aquatic environments, leading to adverse effects on the environment. Furthermore, these insecticides have potentially undesirable side effects on the environment, humans and other non-target species, potentially disrupting entire ecosystems. This study investigated the effects of ethiprole enantiomers against microorganisms in sediment, duckweed, and human cells. The results showed that S-ethiprole degraded preferentially in sediment, demonstrating enantioselective behavior. In sediment, catalase and superoxide dismutase activities were significantly inhibited, and the frond number of duckweed preferably decreased with exposure to S-ethiprole at high concentration treatments (33 : 25 = R-ethiprole: S-ethiprole). Additionally, the content of chlorophyll b decreased nearly 2 times in R-ethiprole (0.42 mg/g) treatment group in comparison to S-ethiprole (0.20 mg/g) treatment. Cytotoxicity test revealed that S-ethiprole was 2 and 3 times more toxic than rac- and R-ethiprole. The expressions of CAT, SOD and caspase-3 were up-regulated in human cells treated with ethiprole enantiomers, indicating oxidative stress as a key factor in toxicity. Overall, S-ethiprole exhibited greater adverse effects than rac- and R-enantiomers in human cells. These findings demonstrate the importance of considering chirality in the risk assessment of harmful chemicals in the aquatic environment.
研究领域:
环境科学
·
毒理学
·
水生生态学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2013
·
IF 0.769
Yuan-Yuan JIANG,Kun WANG,Chong-Zheng XU,Xiao-Di YANG,Hui-Hui LI
In this work, the glassy carbon electrode modified by alizarin (AR) hybridized with graphene (CRG) and chitosan (CS) was prepared. The AR/CRG-CS electrode exhibited a pair of well-defined reversible redox peaks in 0.1 M phosphate buffer solution (PBS, pH 7.4) with the oxidation peak at −0.573 V and the reduction peak at −0.652 V. It exhibited a two-proton and two-electron redox process and a fast heterogeneous electron transfer rate with the rate constant ( K s ) of 1.69 s −1 using the cyclic voltammetry. Based on the characteristics of alizarin with the decreased current and negative potential shift after binding with human telomere DNA, a sensitive, simple, reliable and stable electrochemical method for the detection of human telomere DNA using the AR/CRG-CS electrode was developed. The electrochemical response showed a linear relationship with DNA concentration in the range of 8.0 × 10 −8 −1.4 × 10 −5 M ( R = 0.9995) with the linear regression equation as Δ I pc (μA) = 8.4860 + 0.5366 C (μmol) and the detection limit ( S/N ) as 2.0 × 10 −8 M.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 19
·3512
·
IF 5.719
Zhaofeng Wu,Yidan Xia,Lixiang Liu,Qihua Sun,Jun Sun,Furu Zhong,Min Zhang,Haiming Duan
Waste human hair was carbonized into carbon sheets by a simple carbonization method, which was studied as gas sensing materials for the first time. The effect of carbonization temperature on the structure and gas sensing properties of hair-based carbon sheet was studied by scanning electron microscope, X-ray diffraction, infrared spectrum, Raman spectrum, and gas-sensitive tester. The results showed that the carbonization temperature had a significant effect on the structure and gas sensing performance of carbon sheets, which were doped with K, N, P, and S elements during carbonization. However, the sensor of the carbon sheet does not show good selectivity among six target gases. Fortunately, the carbon sheets prepared at different temperatures have different responses to the target gases. The sensor array constructed by the carbon sheets prepared at different temperatures can realize the discriminative detection of a variety of target gases. For the optimized carbon sheet, the theoretical limit of detection of hydrogen peroxide is 0.83 ppm. This work provides a reference for the resource utilization of waste protein and the development of gas sensors.Keywords:human hair;biomass carbon material;gas-sensitive properties;carbon sheets
研究领域:
材料科学
·
环境科学
·
传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOPHARMACEUTICS & DRUG DISPOSITION
·
2022
·Vol. 43
·期 6
·247-254
·
IF 1.831
Ting Zhu,Yu Wu,Xue-Mei Li,Yu-Meng Jia,Huan Zhou,Li-Ping Jiang,Ting Tai,Qiong-Yu Mi,Jin-Zi Ji,Hong-Guang Xie
As an analog of clopidogrel and prasugrel, vicagrel is completely hydrolyzed to intermediate thiolactone metabolite 2-oxo-clopidogrel (also the precursor of active thiol metabolite H4) in human intestine, predominantly by AADAC and CES2; however, other unknown vicagrel hydrolases remain to be identified. In this study, recombinant human Raf kinase inhibitor protein (rhRKIP) and pooled human intestinal S9 (HIS9) fractions and microsome (HIM) preparations were used as the different enzyme sources; prasugrel as a probe drug for RKIP (a positive control), vicagrel as a substrate drug of interest, and the rate of the formation of thiolactone metabolites 2-oxo-clopidogrel and R95913 as metrics of hydrolase activity examined, respectively. In addition, an IC 50 value of inhibition of rhRKIP-catalyzed vicagrel hydrolysis by locostatin was measured, and five classical esterase inhibitors with distinct esterase selectivity were used to dissect the involvement of multiple hydrolases in vicagrel hydrolysis. The results showed that rhRKIP hydrolyzed vicagrel in vitro, with the values of K m , V max , and CL int measured as 20.04 ± 1.99 μM, 434.60 ± 12.46 nM/min/mg protein, and 21.69 ± 0.28 ml/min/mg protein, respectively, and that an IC 50 value of locostatin was estimated as 1.24 ± 0.04 mM for rhRKIP. In addition to locostatin, eserine and vinblastine strongly suppressed vicagrel hydrolysis in HIM. It is concluded that RKIP can catalyze the hydrolysis of vicagrel in the human intestine, and that vicagrel can be hydrolyzed by multiple hydrolases, such as RKIP, AADAC, and CES2, concomitantly.
研究领域:
药理学
·
生物化学
✓ 已验证
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 11
·2425-2432
·
IF 5.227
Haozhou Jin,Wenqing Gao,Rong Liu,Jiaqian Yang,Shun Zhang,Renlu Han,Jing Lin,Sijia Zhang,Jiancheng Yu,Keqi Tang
Protein glycosylation is of great significance in various physiological processes. Nevertheless, it remains a huge challenge to identify glycopeptides in complex biosamples by the direct mass spectrometry analysis due to the low ion efficiency and low abundance of glycopeptides. In this study, a novel hydrogel (denoted as ZIF-8/SAP) with a stable three-dimensional (3D) network structure and excellent hydrophilicity was successfully fabricated to capture glycopeptides with high efficiency. Owing to the unique characteristics, ZIF-8/SAP exhibited great selectivity (1 : 1000), great sensitivity (1 fmol μL−1), large binding capacity (300 mg g−1) and satisfactory reusability (seven cycles). Inspired by the great enrichment performance of the as-prepared material toward glycopeptides, ZIF-8/SAP was further applied to capture glycopeptides from a real human serum sample. The experimental results demonstrated that 217 N-glycosylation sites were identified in 283 N-glycopeptides, corresponding to 95 glycoproteins identified from 10 μL human serum by the nano-LC-MS/MS analysis, revealing the great potential of the novel ZIF-8/SAP hydrogel for glycopeptide enrichment and glycoproteomic research.
研究领域:
蛋白质组学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Zhiyuan Wang,Renfeng Liu,Youjun Liu,Yuqi Zhao,Yuhao Wang,Botao Lu,Hui Li,Cheng Ju,Weidong Wu,Xinlin Gao,Hailiang Xu,Shixiang Cheng,Yulin Cao,Shuaijun Jia,Chunping Hu,Lei Zhu,Dingjun Hao
Spinal cord organoids are of significant value in the research of spinal cord-related diseases by simulating disease states, thereby facilitating the development of novel therapies. However, the complexity of spinal cord structure and physiological functions, along with the lack of human-derived inducing components, presents challenges in the in vitro construction of human spinal cord organoids. Here, we introduce a novel human decellularized placenta-derived extracellular matrix hydrogel (DPECMH) and, combined with a new induction protocol, successfully construct human spinal cord organoids. The human placenta-sourced decellularized extracellular matrix (dECM), verified through hematoxylin and eosin staining, DNA quantification, and immunofluorescence staining, retained essential ECM components such as elastin, fibronectin, type I collagen, laminin, and so forth. The temperature-sensitive hydrogel made from human placenta dECM demonstrated good biocompatibility and promoted the differentiation of human induced pluripotent stem cell (hiPSCs)-derived spinal cord organoids into neurons. It displayed enhanced expression of laminar markers in comparison to Matrigel and showed higher expression of laminar markers compared to Matrigel, accelerating the maturation process of spinal cord organoids and demonstrating its potential as an organoid culture substrate. DPECMH has the potential to replace Matrigel as the standard additive for human spinal cord organoids, thus advancing the development of spinal cord organoid culture protocols and their application in the in vitro modeling of spinal cord-related diseases.
研究领域:
干细胞生物学
·
神经科学
·
组织工程
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 8
·3607-3618
·
IF 3.9
Liang He,Jie Zhang,Leiding Shen,Xiaofeng Ji,Rui Li
We investigated the occurrence of 80 pesticide residues in 96 honey samples from apiaries with honeybee poisoning incidences by liquid chromatography–tandem mass spectrometry and subsequently conducted risk assessments of exposure for in-hive honeybees and Chinese consumers. Six pesticides were detected with residue concentrations ranging from 0.5 to 130.9 µg/kg. The mean concentrations of acetamiprid, dinotefuran, hexythiazox, propargite, semiamitraz, and carbendazim in positive samples were 7.9 ± 9.1, 5.9 ± 1.7, 3.0 ± 1.6, 44.2 ± 50.0, 9.0 ± 9.4, and 5.5 ± 4.1 µg/kg, respectively. Carbendazim, semiamitraz, and acetamiprid were the major contaminants in honey, with incidences of 99.0%, 93.8%, and 49.0%, respectively. The cooccurrence of pesticides (≥2 pesticides) was detected in 95.9% of the samples, with up to six residual pesticides found in one sample. The HQ (hazard quotient) values of the six pesticides to in-hive honeybees were from 4.7 × 10 −8 to 0.021, less than 1, indicating their acceptable exposure risk to honeybees. In terms of the representative-case and worst-case scenarios, the sum of separate HQs of each pesticide yielding an HI (hazard index) ranged from 0.012 to 0.016 for in-hive worker honeybees and from 0.015 to 0.021 for in-hive larva honeybees, indicating an overall acceptable potential cumulative risk of multiple pesticides to in-hive honeybees. Both the %ARfD (acute reference dose) value (0.0001–0.075) and %ADI (acceptable daily intake) value (0.00002–0.0046) of risky pesticides were much less than 100, revealing acceptable risk exposure to risky pesticides via honey consumption for human health. Thus, our results showed that multipesticide residual honey from apiaries with honeybee poisoning incidents in East China was safe for humans and in-hive honeybees. Practical Application This analytical approach will be used in detecting multiple pesticide residues in honey and risk assessment for dietary exposure to pesticide residues. It can support various surveillance programs about honey safety and in-hive honeybee health evaluation.
研究领域:
环境科学
·
食品科学
·
昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Zhongnan Huang,Zhenglian Li,Luyao Xu,Chaoguo Wei,Chenting Zhu,Haohua Deng,Huaping Peng,Xinghua Xia,Wei Chen
Monitoring nicotine concentrations in human fluids is extremely crucial owing to the harmful effect of nicotine on human health. Herein, it is shown that nicotine could quench the cathodic electrochemiluminescence (ECL) of gold nanoclusters (AuNCs) with high efficiency. The ECL quenching mechanism of nicotine was studied in detail using various experimental tools and theoretical calculations. It was concluded that the strongly oxidizing intermediate SO4•–, produced from K2S2O8, could oxidized nicotine, resulting in ECL emission quenching. On the basis of this high-efficiency ECL quenching of the AuNCs/K2S2O8 system, a recyclable, ultrasensitive, and selective ECL sensing platform for nicotine detection was proposed. Even in the absence of any complex signal amplification techniques, the ECL sensor for nicotine detection showed an unprecedentedly low detection limit of 7.0 × 10–13 M (S/N = 3) and a wide linear range over 8 orders of magnitude. Most remarkably, it could be successfully used for nicotine detection in human urine samples. This is expected to promote the investigations and applications on nicotine-related diseases. We believe that the proposed ECL platform can hold great prospects for commercialization in biomedical fields and tobacco industries.
研究领域:
分析化学
·
生物医学工程
·
烟草科学
✓ 已验证
DOI
高影响因子
FIBERS AND POLYMERS
·
2021
·Vol. 22
·期 1
·141-148
·
IF 2.153
Jia Weini,Li Shirong,Luo Zhengxin,Yu Hainan,Zhu Wenjun,Mao Qinghui,Zhang Xiaoli,Liang Zhijie,Dong Aixue
Due to the damage of scalp because of chemical dyes, increasing attention had been paid to the ecological dyeing of human hair. To address the need for ecological restoration and dyeing of human hair, herein, a novel approach has been proposed via laccase-mediated in situ oxidation of dihydroxy phenylalanine (dopamine). As laccase can catalyze dopamine oxidation and then polymerization to produce strongly colored polymers, different biological coloration processes of human hair based on this process were investigated: (i) Human hair was treated with laccase alone; (ii) The absorption of human hair with dopamine, followed by further catalysis of air; (iii) The absorption of human hair with dopamine, followed by further catalysis of laccase; (iv) Simultaneous laccase-mediated polymerization and dyeing of human hair; and (v) Polymerization of Dopamine with laccase and then dyeing human hair. The surface properties of dyed hair were evaluated in terms of ultraviolet (UV)-protection, color fastness, Fourier transforming infrared spectrum (FT-IR), optical microscope, and scanning electron microscopy (SEM). The results show that best hair dyeing effect was achieved via simultaneous laccase-mediated polymerization and dyeing. The dyed hair obtained K/S values of 28, light fastness of level 5, and UPF of 100 + . The optical microscope and SEM observations show that the surface of colored hair fibers was covered with thin burrs, which can be attributed to polydopamine attached with physical adhesive force and covalent reaction. FT-IR analysis shows that functional groups were present on the surface of the dyed hair which can be considered as introduction of hydroxyl radical groups. This biological stain technique may be widely used in dyeing of hair with black color.
研究领域:
生物学
·
生物化学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Hongmei Zhang,Hao Deng,Yanqing Wang
In this work, the binding interaction of an artificial sweetener, acesulfame (ACS) with human serum albumin (HSA) are investigated at the molecular level by using spectral methods and molecular modeling. ACS has the ability to induce static quenching of the intrinsic fluorescence of HSA by a complex formed between HSA and ACS through weak multi-noncovalent forces including hydrophobic, hydrogen bond and van der Waals forces . ACS enters subdomain IIA of HSA to induce the tertiary structure changes of HSA and decreased the hydrophobicity of protein. In addition, ACS binding does not obviously alter the secondary structure of HSA. This study is hoped to provide some crucial information for further investigations of the biosafety of sweetener.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
Toxics
·
2024
·Vol. 12
·期 4
·286
·
IF 3.9
Wangxing Luo,Siyu Yao,Jiahui Huang,Haochuan Wu,Haijun Zhou,Mingjiang Du,Ling Jin,Jianteng Sun
Organophosphate esters (OPEs) are frequently used as flame retardants and plasticizers in various commercial products. While initially considered as substitutes for brominated flame retardants, they have faced restrictions in some countries due to their toxic effects on organisms. We collected 37 soil and crop samples in 20 cities along the coast of South China, and OPEs were detected in all of them. Meanwhile, we studied the contamination and potential human health risks of OPEs. In soil samples, the combined concentrations of eight OPEs varied between 74.7 and 410 ng/g, averaging at 255 ng/g. Meanwhile, in plant samples, the collective concentrations of eight OPEs ranged from 202 to 751 ng/g, with an average concentration of 381 ng/g. TDCIPP, TCPP, TCEP, and ToCP were the main OPE compounds in both plant and soil samples. Within the study area, the contaminants showed different spatial distributions. Notably, higher OPEs were found in coastal agricultural soils in Guangdong Province and crops in the Guangxi Zhuang Autonomous Region. The results of an ecological risk assessment show that the farmland soil along the southern coast of China is at high or medium ecological risk. The average non-carcinogenic risk and the carcinogenic risk of OPEs in soil through ingestion and dermal exposure routes are within acceptable levels. Meanwhile, this study found that the dietary intake of OPEs through food is relatively low, but twice as high as other studies, requiring serious attention. The research findings suggest that the human risk assessment indicates potential adverse effects on human health due to OPEs in the soil–plant system along the coast of South China. This study provides a crucial foundation for managing safety risks in agricultural operations involving OPEs.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）