RSC Advances
·
2017
·Vol. 7
·期 55
·34746-34754
·
IF 3.108
Ziwei Hu,Mingshan Xie,Danting Yang,Dong Chen,Jingyi Jian,Haibin Li,Kaisong Yuan,Zhengjin Jiang,Haibo Zhou
Berberine is an important anti-inflammatory drug widely used for the treatment of heart and intestinal disorders. The quantification of berberine is usually required in clinic medical assays. Herein, we report simple, fast and sensitive colorimetric visualization of berberine in human plasma using citrate-stabilized gold nanoparticles (AuNPs). Upon exposure to μg mL−1 levels of berberine, the AuNP solution exhibits a highly sensitive color change from red to blue and rapid aggregation kinetics within the initial 10 min, which can be directly seen with the naked eye and monitored by UV-vis absorbance spectroscopy. As confirmed by comparison with potential coexisting foreign substances, the presence of positively charged berberine induces the aggregation of the AuNPs owing to the electrostatic attraction and coordination interactions between the ring nitrogen atoms of berberine and the AuNPs, causing a color change of the AuNP suspension. Further evidence is that the sensitivity is significantly improved when NaHSO4 is added to promote the ligand exchange between citrate and berberine at the surface of the AuNPs. The NaHSO4-optimized AuNP system provides a simple colorimetric assay for the rapid detection of berberine in human plasma. The limit of detection (LOD) is 0.24 μg mL−1 by the naked eye and 0.06 μg mL−1 by UV-vis spectroscopy.
研究领域:
药理学
·
分析化学
·
纳米医学
✓ 已验证
DOI
高影响因子
Bioanalysis
·
2016
·Vol. 8
·期 13
·
IF 2.813
Qinghua Wang,Yan Wen,Tianyi Xia,Xiaojuan Xiong,Shouhong Gao,Chunhua You,Xia Tao,Feng Zhang,Wansheng Chen
Aim: Quantification of amino acids in human plasma has become an important and essential analysis parameter in life science. In this paper, we developed a targeted UHPLC–MS/MS method for 18 amino acids in the renal transplant patients. Methods & results: Plasma in small volume (150 μl) was pretreated by a one-step protein precipitant extraction for analysis. Detection was executed by MS/MS in the MRM mode. Assays were validated according to current bioanalytical guidelines, with good linearity (R > 0.99), intraday and interday precision (CV < 11.6%, RE ≤ ± 14.8%), extraction recovery (between 77.4 and 117.6%), matrix effect (73.3–118.0%) and stability (RE≤ ±14.7%). Conclusion: The method was successfully applicable for amino acid analysis in the renal transplant patient.
研究领域:
生物分析
·
医学检测
·
临床化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Doudou Zhang,Yu Zhao,Dandan Lan,Xiaomin Pang,Ligai Bai,Haiyan Liu,Hongyuan Yan
In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50 × 4.6 mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N , N -dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis , scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively. Results showed that the homemade monolith occupied relatively uniform pore structure , low back pressure, and enhanced selectivity for proteins in complex bio-samples. The present work described a simple and efficient method for “fractionation separation” of human plasma proteins , and it is a promising separation method for complex bio-samples in proteomic research.
研究领域:
蛋白质组学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Lingfang Dong,Kun Li,Shan Gao,Wenyi Wang,Yinghua Feng,Jingying Zhang,Jie Yang,Weifeng Dong,Wei Zhang
we developed an effective protein precipitation method for determination of levamlodipine in human plasma using LC–MS/MS. Sample extraction was carried out by using liquid–liquid extraction in 96-well plate format. (S)-Amlodipine-d4 was used as internal standard (IS). The chromatographic separation was achieved using Philomen Chiral MX (2) column (3 μm, 2.1 × 100 mm). Mobile phase A was comprised of Acetonitrile (ACN), Mono ethanol amine (MEA) and Iso-Propyl alcohol (IPA) (1000:1:10, v/v/v), Mobile phase B was IPA-ACN (2:1, v/v). The flow rate was 0.4 mL/min. The total run time of each sample was 4.0 min with gradient elution. LC-MS/MS spectra were generated in positive ion mode, and multiple reaction monitoring (MRM) was used to detect the following transitions: m/z 409.20 → 238.15 for levamlodipine and 415.25 → 240.20 for (S)-Amlodipine-d4 (the IS). The method was linear from 50 to 10000 pg/mL（R 2 ＝0.9988489）,and the lower limit of quantification (LLOQ) was 50 pg/mL. This method was applied to a bioequivalence study of levamlodipine.
研究领域:
药物分析
·
临床药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2013
·Vol. 52
·期 10
·1173-1180
·
IF 0.794
Zhang Jin-Feng,Zhang Zhi-Qing,Dong Wei-Chong,Jiang Ye
A novel and sensitive high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of valproic acid (VPA) in human plasma. The method was based on derivatization of VPA using 2-bromo-2′-acetonaphthone as a new derivatization reagent. Caprylic acid was used as an internal standard (IS). Under the optimized extraction and derivatization conditions, the method showed good linearity in the range of 0.05–200 μg mL−1 and the limit of detection was as low as 0.01 μg mL−1. The relative standard deviation for intra-day and inter-day (n = 5) was <5%. The recovery ranged from 95.2 to 101.4%. The proposed method is proved to be highly sensitive, simple and rapid, and was successfully applied to the analysis of VPA in plasma samples from patients with generalized epilepsy.
研究领域:
分析化学
·
医学检验
·
药代动力学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Fang Wei,Xiang Wang,Hui-fang Ma,Xin Lv,Xu-yan Dong,Hong Chen
In this study, we developed a novel strategy using solid-phase extraction (SPE) coupled with shotgun mass spectrometry (MS) based on trimethylsilyldiazomethane (TMSCHN 2 ) stable-isotope derivatization for rapid profiling and accurate quantification of phospholipids (PLs) in human plasma. HybridSPE-Phospholipid (HybridSPE-PL, zirconia coated silica stationary phase) was used for sample pretreatment via the Lewis acid-base interaction between zirconia and phosphate moiety of PLs. This step allows rapid enrichment and recovery of PLs from human plasma. Afterward, PLs were derivatized with TMSCHN 2 , which leads to methylation of hydroxyl and amino groups in PLs and allows highly sensitive PL analysis by shotgun MS in positive ionization mode (limit of detection decreased up to 116.67 fold compared to underived PLs). We developed an accuracy quantification method for determination of PL molecular species in biological samples. Two or more PL standards were selected for each PL class and derivatized with TMSCHN 2 without stable-isotope coding. They were then used as the internal standards. PLs in biological samples were isotopic derivatized via acid-catalyzed H/D exchange and methanolysis of TMSCHN 2 . For accurate quantification, a calibration curve for each class of PLs was typically constructed by using the internal standards to normalize the non-uniformity response caused by the differential fragmentation kinetics resulting from the distinct chemical constitution of individual PL species in the biological samples. This newly developed method was used to comprehensively analyze PL molecular species in human plasma samples. It is a promising methodology for rapid profiling and accurate quantification of complex lipid molecules in biological samples.
研究领域:
生物化学
·
医学检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 17
·5509
·
IF 4.927
Shan-Yan Liang,Fang Shi,Yong-Gang Zhao,Hong-Wei Wang
In this work, magnetic tetraethylenepentamine (TEPA)-modified carboxyl–carbon nanotubes were synthesized, characterized, and used as adsorbents to conduct magnetic solid-phase extraction (MSPE) for the preconcentration of seven local anesthetic drugs (procaine, lidocaine, mepivacaine, oxybuprocaine, bupivacaine, tetracaine, and cinchocaine) from human plasma. The separation and determination of analytes were performed on high-performance liquid chromatography with UV detection. Several factors affected the extraction efficiency, such as the amount of adsorbents used, extraction time, sample pH, and optimization of elution conditions. Under optimal conditions, satisfactory linear relationships were obtained in the range of 0.02–5.00 mg/L, with the limits of detection (LOD) ranging from 0.003 mg/L to 0.008 mg/L. The recoveries of analytes for spiked human plasma were in the range of 82.0–108%. Moreover, the precision with intra-day and inter-day RSD values were obtained in the range of 1.5–7.7% and 1.5–8.3%. The results indicated that this method could determine the concentration of seven local anesthetic drugs in human plasma with high precision and repeatability and provide support for the clinical monitoring of the concentration of local anesthetic drugs in human plasma.Keywords:HPLC;MSPE;local anesthetic drugs;human plasma
研究领域:
分析化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Xiaoqian JIANG,Xinxin XU,Shanshan SONG,Hua KUANG,Liqiang LIU,Liguang XU,Chuanlai XU
Digitoxin is a cardiac glycoside used in the therapy of heart failure and arrhythmias, and has potential as an anti-cancer and anti-influenza treatment. However, digitoxin has the disadvantage of a narrow therapeutic window meaning that it is easy to cause toxicity. Consequently, there is an urgent need to accurately monitor the plasma concentration of patients. In this study, an anti-digitoxin monoclonal antibody (mAb) 5D2 was developed and purified, and found to have outstanding specificity, sensitivity, and affinity. The half inhibitory concentrations (IC 50 ) and the limit of detection (LOD) values of mAb 5D2 in human plasma determined by ic-ELISA were 0.205 ng/mL and 0.010 ng/mL, while the cross-reactivity with other analogs was negligible. Based on mAb 5D2, a colloidal gold-based immunochromatographic assay was developed for the purpose of quickly monitoring the concentration of digitoxin in human plasma. The detection results could be obtained within 10 min, and were in agreement with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The strip could be evaluated semi-quantitatively by the naked eye, with a visual LOD (vLOD) of 0.5 ng/mL and a cut-off value of 10 ng/mL. Meanwhile, the linear range was between 0.319 ng/mL and 2.406 ng/mL, which was obtained quantitatively by the strip reader. Therefore, the immunochromatographic strip could offer a new tool for rapid, accurate, and portable detection of digitoxin.
研究领域:
医学
·
药理学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Rindia M. Putri,Daquan Zhang,Ilona Joan Manoela,Henty Tri Agustina,Grace Wara Patiung,Alfredo Kono,Diah Hardianti,Jun Xian Goh,Yanti Rachmayanti,Zeily Nurachman,Aijie Liu
Upon blood contact, biomaterials rapidly adsorb plasma proteins, forming a protein corona that dictates their biological identities and interactions. While diatom biosilica presents a naturally derived, nanostructured platform for biomaterial applications, its interaction with human plasma at the biomolecular level remains unknown. Here, we investigate the formation dynamics and proteomic composition of the protein corona on diatom biosilica. At the initial stages, we compared two tropical marine diatom species: Cyclotella striata TBI (centric) and Navicula salinicola NLA (pennate). Starting with human serum albumin (HSA) as a model protein and then transitioning to human plasma, we demonstrated that N. salinicola biosilica exhibited a higher capacity for protein adsorption, likely due to its elongated morphology and larger pores. Adsorption occurred immediately and followed pseudo-first-order kinetics, reaching equilibrium within ∼20 min, with HSA predominantly localizing on the biosilica outer surface, based on tomographic confocal imaging. LC-HRMS proteomic analysis revealed that plasma interactions with biosilica induced HSA depletion and selective enrichment of immunoglobulins, homeostatic proteins, and apolipoproteins in the corona. Analyses of protein parameters (i.e., pI and GRAVY values) for the enriched proteins indicate a stronger affinity of biosilica toward hydrophilic and positively charged plasma proteins. Functional annotation of the enriched proteins suggests that biosilica might play a role in immune activity modulation, complement activation, iron homeostasis, as well as the regulation of coagulation and wound healing. These findings unveiled fundamental biomolecular insights governing biosilica-protein interactions, paving the way toward the integration of diatom biosilica into biomaterial engineering, diagnostics, and therapeutic applications.
研究领域:
生物材料科学
·
蛋白质组学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Jun-Tao Cao,Wen-Sheng Zhang,Xiao-Long Fu,Hui Wang,Shu-Hui Ma,Yan-Ming Liu
A high-efficient chemiluminescence (CL) platform for highly selective and sensitive H 2 S detection was constructed on the basis of the quenching effect of S 2 − on the copper ion modified graphitic carbon nitride nanosheets (Cu 2+ -g-C 3 N 4 NSs) enhanced luminol-H 2 O 2 system. Cu 2+ -g-C 3 N 4 NSs with horseradish peroxidase-like catalytic activity were prepared and provide a great improvement for luminol-H 2 O 2 system. The presence of S 2 − induced the formation of CuS precipitate on g-C 3 N 4 NSs surface. The precipitate can block the catalytic Cu 2+ sites on the g-C 3 N 4 NSs surface, resulting in a great CL decrease of CL system. Based on such a mechanism, a simple, highly selective and sensitive CL biosensor for H 2 S detection was designed. Under the optimized conditions, luminol-H 2 O 2 -Cu 2+ -g-C 3 N 4 NSs system gave a decrease of CL intensity with the Na 2 S concentration increasing. The CL biosensor is in a linear range of 10.0 pM–50.0 nM and the detection limit for detecting Na 2 S is as low as 2.0 pM. Moreover, the method here has enjoyed a successful application for determining H 2 S in human plasma samples and the recovery is between 95.7% and 110.0%.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2012
·
IF 3.794
Shuchao Wu,Wei Xu,Qamar Subhani,Bingcheng Yang,Deying Chen,Yan Zhu,Lanjuan Li
This paper describes the determination of carbamazepine (CBZ) in human plasma using ion chromatography combined with online electrochemical derivatization and fluorescence detection. Separation of CBZ with anion exchange column was demonstrated to be feasible using either basic (10 mM NaOH) or acidic (0.1 M H 3 PO 4 ) reagent with a small amount of acetonitrile (ACN) added as eluent . Electrochemical derivatization of CBZ into a strongly fluorescent product, which could be carried out only under the acidic condition, was investigated via the previously reported electrolytic cell (EC), as well as two modes of acidification . The linear range of CBZ for human plasma was between 10–2000 μg L −1 under the optimized experimental conditions. The limit of detection (LOD, S/N=3) was 1.3 μg L −1 and the relative standard deviation (RSD, n =7) was 2.6%. Better sensitivity and selectivity of the present method were demonstrated in comparison with ion chromatography with ultraviolet detection (IC-UV). The spiked recoveries of CBZ in 2 human plasma samples were 78.5 and 114%, respectively.
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 16
·6358-6368
·
IF 1.938
Chao Kang,Hai-Long Wu,Shou-Xia Xiang,Li-Xia Xie,Ya-Juan Liu,Yong-Jie Yu,Jing-Jing Sun,Ru-Qin Yu
A practical analytical method based on intrinsic fluorescence is proposed for simultaneous determination of L-phenylalanine, L-tyrosine, and L-tryptophan in cell culture and human plasma. By using a three-way calibration method coupled with excitation–emission matrix fluorescence, the proposed method successfully achieved quantitative analysis of the three aromatic amino acids in the two different complex systems simultaneously, even in the presence of three unknown, uncalibrated serious interferents. The method needs little preparation by using “mathematical separation” instead of chemical or physical separation, which makes it efficient and environmentally friendly. Satisfactory results have been achieved for calibration, validation, and prediction sets. For phenylalanine, tyrosine, and tryptophan, the calibration ranges are 6.00 to 60.00, 0.40 to 4.00, and 0.10 to 1.00 μg mL−1 respectively. The average spike recoveries (mean ± standard deviation) are 98.5 ± 7.8%, 103.7 ± 6.9%, and 102.3 ± 7.9% respectively. The relative errors are −4.2%, 6.3%, and −0.8% for predicting real contents of phenylalanine, tyrosine, and tryptophan in cell culture respectively. Additionally, we discussed the potential of the three-way calibration method for determining analytes of interest in different systems simultaneously, to further explore the second-order advantages. The paired t-test results indicate that the predicted results between prediction in two systems simultaneously and prediction in a single system individually have no significant difference. The satisfactory results obtained in this work indicate that the use of the three-way calibration method coupled with the EEM array is a promising tool for multi-component simultaneous determination in multiple complex systems containing uncalibrated spectral interferents.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）