JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Xindi Wang,Jiangshan Xue,Jiutong Ma,Hai Wang,Qiong Jia
Transferrin (TRF), a glycoprotein involved in cellular iron uptake, is a potential target for the diagnosis and treatment of several diseases and cancers. Therefore, the identification and isolation of TRF is clinically important. In this work, we prepared magnetic molecularly imprinted polymers (EMIP) based on metal chelation using norepinephrine and 3-aminophenylboronic acid as functional monomers. The obtained EMIP shows excellent recognition of TRF with the adsorption capacity of 94.2 mg/g and imprinting factor of 3.50. In addition, EMIP was characterized by high specificity, good adsorption performance and stability, and was successfully used for the analysis of TRF in human serum. The present study provides a reliable scheme for targeted epitope imprinting of polymers with metal chelating and dual-functional monomers, showing great potential for biosample analysis.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yajie Fan,Jiangxue Dong,Junyao Wang,Na Li,Saimei Zhang,Yilin Zhang,Yanlei Li,Ming Su,Hongyuan Yan,Shigang Shen,Zhongfeng Gao
While uricase enzyme therapy for gout effectively reduces uric acid (UA) levels, it can lead to the accumulation of hydrogen peroxide (H 2 O 2 ) within the organs, potentially inducing cellular oxidative stress and additional health issues. Therefore, addressing the degradation of UA along with the consumption of H 2 O 2 is a critical challenge to be resolved. This study reports a flower-shaped heterometallic PdCu 3 nanozyme with exceptional dual enzymatic activity, mimicking both uricase and peroxidase to effectively degrade UA and consume H 2 O 2 . The heterometallic PdCu 3 nanozyme demonstrated significant efficacy in degrading UA within a high degradation rate of ∼77.0 %. Compared to conditions where only Cu 2 O or Pd nanozyme is present, the degradation efficiency is enhanced by factors ranging from 1.1 to 3.8 times. The PdCu 3 -H 2 O 2 –3,3′,5,5′-tetramethylbenzidine chromogenic system was developed for the quantitative detection of UA, leveraging ultraviolet-visible spectrophotometry and smartphone-based colorimetry. Compared with the reporting methods, this study has the following advantages: a wider linear range, shorter reaction time, and no requirement for specific temperature conditions, allowing it to be conducted at room temperature. To validate the practical reliability of the detection method, spiking recovery experiments in human serum were performed, yielding a recovery rate of 98.1 %–101.4 % with a relative standard deviation of less than 5 %, confirming good precision and accuracy in complex biological matrices. This study exhibits the potential of PdCu 3 nanozymes in biomedical applications, offering potential as a candidate for further exploration in biomedical research.
研究领域:
纳米酶
·
生物催化
·
生物传感器
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Wang Pan,Zhou Zheng,Xingxing Wu,Jiaojiao Gao,Yani Liu,Qunhui Yuan,Wei Gan
The development of transition metal based enzyme-free glucose (Glu) sensor with high sensitivity, wide linear range, and long-term stability is highly desired. Herein, a series of 2D/3D hierarchical structured bimetallic organic framework (NiCu-MOF- x , x = 2, 4, 6, 8) were developed through a one-step hydrothermal route by adjusting the ratio of nickel and copper salts. The morphological similarity of NiCu-MOF- x and Ni-MOF was evidenced by X-ray diffraction survey. The structural evolution of NiCu-MOF- x was revealed by scanning electron microscopy. The optimal NiCu-MOF-6 exhibits high electrocatalytic activity toward Glu oxidation. Being applied as the electrode modifier for glassy carbon electrode (GCE), NiCu-MOF-6/GCE exhibits a remarkably widened linear range than its monometallic analog, owing to its significantly increased electrochemical surface area, interconnected channels for mass/electron transfer and synergistically functionalized Ni/Cu electroactive sites. Being applied in human serum analysis, this sensor shows a good feasibility with satisfying recoveries.
研究领域:
材料科学
·
电化学
·
传感器
·
分析化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Peigang Zhang,Yunhui Fu,Tianyi Zheng,Huihui Ge,Martin M.F. Choi,Li Zhang,Wei Bian
Glutathione (GSH), an essential biothiol, plays a critical role in reversible redox reactions, cellular signaling, detoxification, and metabolism. Fluctuations in GSH levels are closely linked to serious health conditions, underscoring the significance of monitoring its concentration. Fluorescence-based sensing with nanomaterials offers a promising strategy for rapid GSH detection. In this work, we developed a fluorescent probe integrating 7-amino-4-methylcoumarin (AMC) and cobalt oxyhydroxide nanoflakes (CoOOH NFs) for GSH detection through a combined internal-filter effect and static quenching mechanism. The introduction of GSH triggered a redox reaction that degraded CoOOH NFs, suppressing their quenching effect and restoring AMC fluorescence, which enabled GSH quantification through fluorescence recovery. The probe detected GSH in the range of 20–200 μM with a detection limit of 0.13 μM. It was successfully applied to determine GSH in human serum, and the results showed excellent agreement with those measured by a commercial GSH detection kit based on the use of Ellman's reagent.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
传感器
·
荧光光谱学
✓ 已验证
产品关联
DOI
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 3
·125-129
·
IF 0.975
Chulei Zhao,Kaihang Cao,Chaoyun Ma,Wenjun Li,Yiju Song,Xiuwen Qiao,Chenglin Hong
In this experiment, a Cu 2 O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml ( S / N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.
研究领域:
分析化学
·
纳米科技
·
生物化学
·
生物医学工程
·
电化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）