Advanced Healthcare Materials
·
2025
·
IF 9.6
Weibo Zhang,Chong Chen,Ran Wang,Yixuan Li,Yinhua Zhu,Jiazeng Sun,Yongting Luo,Junjie Luo,Liang Zhao,Yanan Sun,Huiyuan Guo,Hao Zhang,Bing Fang,Yao Hu,Rong Liu,Xiaoyu Wang,Jiayue Guo,Ping Liu,Peng An,Pengjie Wang,Juan Chen,Fazheng Ren
Human serum albumin (HSA) plays an indispensable role as a carrier of endogenous and exogenous substances in vivo. The efficiency of endocytosis determines the transport capacity. However, the potential for enhancing the efficiency of HSA endocytosis remains largely unknown. This research represented a substantial advancement, revealing that Fe 2+ markedly increased HSA endocytosis. Moreover, Fe 2+ facilitated the endocytosis and transport of HSA in vivo. The enhancement in HSA uptake facilitated by Fe 2+ is markedly decreased following iron chelation, indicating a specific interaction between Fe 2 ⁺ and HSA that promoted endocytosis. Moreover, the study elucidated that FcRn and caveolin regulated HSA endocytosis under normal conditions. Yet, in the presence of Fe 2 ⁺, endocytosis shifted toward clathrin- and caveolin-enriched membrane domains, requiring both divalent metal transporter 1 (DMT1) and FcRn for efficient process completion. Thereby a new pathway is discovered for Fe 2+ -dependent HSA endocytosis involving DMT1 and clathrin. This findings highlighted the crucial role of iron in enhancing HSA endocytosis and transport, establishing Fe 2+ as a vital endocytic enhancer that required binding to HSA to substantially improve its uptake. This insight into the mechanism of HSA endocytosis not only expands the understanding of protein transport but also opens new avenues for therapeutic intervention.
研究领域:
生物化学
·
细胞生物学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Minghui Zan,Shuai An,Lei Cao,Yulu Liu,Li Li,Mingfeng Ge,Pai Liu,Zaihui Wu,Wen-Fei Dong,Qian Mei
Progesterone (P4) is an important steroid hormone that act as an indicator in the female reproductive processes; thus, following its concentration levels in human serum is important for diagnosis of early pregnancy. In this work, carbon dots (CDs) were designed via a dry-heating method, and the novel CDs exhibited good dispersion stability and excellent photostability. Under optimal conditions, the as-prepared CDs displayed high selectivity and sensitivity for P4 and exhibited a fast-responsive (within 1 min) fluorescence intensity enhancement signal in aqueous solution. For further application, the fluorescence sensing system was further extended to monitoring progesterone in real samples with acceptable recoveries. The methodology developed here offers a promising direction for the detection of clinical biomarkers that are closely related to human health.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Chao Xia,Yan Xu,Meng-Meng Cao,Yun-Peng Liu,Jin-Feng Xia,Dan-Yu Jiang,Guo-Hong Zhou,Rong-Jun Xie,Da-Feng Zhang,Hui-Li Li
In the present study, a kind of Eu(III) post-functionalized Zr(IV)-based metal-organic framework (UiO-66(COOH) 2 , Zr-MOF: Eu 3+ ) was synthesized and utilized as an independently luminescent probe for sensing bilirubin (BR) in human serum, a biomarker of jaundice hepatitis. It can be served as a turn-off fluorescent switch for BR because its red emission from Eu 3+ can be easily quenched by BR through a fluorescent resonant energy transfer (FRET) process between BR and its ligands, and as a result, BR is recognized successfully. Particularly, Zr-MOF: Eu 3+ has shown many appealing properties, such as high sensitivity, quick response (less than 1 min), broad response window (0–15 μM), and excellent selectivity. Most importantly, a kind of portable test paper based on Zr-MOF: Eu 3+ probe has been developed for directly assessing the level of BR in real human serum and further diagnosing bilirubin-related diseases via visually observing the luminescent color variation.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NPG Asia Materials
·
2022
·Vol. 14
·期 1
·1-12
·
IF 10.761
Shen Peiyan,Li Mengru,Li Ran,Han Bo,Ma Haojie,Hou Xueyan,Zhang Yuqi,Wang Ji-Jiang
Smart photonic hydrogels based on two-dimensional photonic crystals (2DPC) provide a promising sensing platform for constructing novel chemical and biological sensors due to their facile optical signal readout and highly sensitive responsivity toward target analytes. Aptamers, as recognition elements with high selectivity and affinity, are extensively used to construct a variety of sensors. Herein, we developed two partially base complementary aptamer-functionalized 2DPC hydrogels as aptasensors for the detection of thrombin (TB) in human serum. The photonic hydrogel aptasensors swelled upon exposure to TB solution, leading to an increase in the particle spacing of the 2DPCs. The particle spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the 2DPCs without the requirement of sophisticated instruments. The aptasensor swelling resulted from the decrease in the hydrogel cross-linking density induced by the specific binding between one of the aptamers and TB and the increase in hydrogel mixing free energy induced by the introduction of TB. The particle spacing increase of the optimized aptasensor was linear over the TB concentration range of 1–500 nM, and the limit of detection was 0.64 nM. The constructed 2DPC hydrogel aptasensor was used to detect TB in human serum and achieved recoveries of 95.74–104.21% and a relative standard deviation of 2.52–6.58%, showing the practicability and accuracy of the sensor. The aptamer-actuated 2DPC hydrogel biosensor provides a new strategy for designing other target molecule-sensitive aptasensors, showing great potential for development into home kits.
研究领域:
生物传感
·
材料科学
·
医学检测
✓ 已验证
DOI
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2019
·
IF 4.462
Bolin Lian,Mingfang Wu,Ziqi Feng,Yiping Deng,Chen Zhong,Xiuhua Zhao
This work investigated the preparation of specific targeted drug delivery systems in cancer chemotherapy by folate conjugated human serum albumin nanoparticles encapsulated resveratrol (RES) nanoparticles (FA-HSA-RESNPs). FA was coupled to HSA, and RES was encapsulated in FA-conjugated HSA by high pressure fluid nano-homogeneous emulsification. The average particle size and polydispersity index of NPs prepared under optimal conditions were 102.1 ± 4.9 nm and 0.001. The drug capsulation efficiency and drug loading efficiency were 98.36 and 14.66%, respectively. The analysis of the results of the physical characterization showed that the RES was present in the FA-HSA-RESNPs in an amorphous state. In vitro drug-release study showed that the NPs can release the drug persistently and slowly. The inhibition rate of FA-HSA-RESNPs and RES was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide method to be 110.8 and 157.2 μM, respectively. The targeting ability of the FA-HSA-RESNPs for the HepG2 cell was measured by fluorescein isothiocyanate-modified albumin techniques. The uptake rate of FA-HSA-RESNPs was higher than that of the original RES. By using near-infrared imaging, in vivo activity was labeled with Cy5 fluorescent FA-HSA-RESNP confirmed FA-HSA-RESNP tumor-targeting ability. The intravenous administration bioavailability of FA-HSA-RESNPs was about 5.95-fold higher than that of the original RES.
研究领域:
药学
·
肿瘤学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yushuang Hong,Zhengpei Nie,Xuelian Tian,Jingfang Sun,Qiuju Zhou,Wenbin Liang,Shihong Chen,Jin Huang,Kejun Tan,Lin Dong
In this work, up- and down-conversion dual-emission CDs without rare-earth (UD D-CDs) were synthesized using RhB and 1,4-Diaminoanthraquinone as precursors. The synthesized UD D-CDs exhibited dual emissions at 496 and 580 nm under 260 and 865 nm excitation, respectively. The fluorescence emission mechanism, including contributions from carbon nuclei, surface states, molecular states, and internal defect states, was discussed through the separation and purification of UD D-CDs. Based on the interaction between UD D-CDs and copper ions (Cu 2+ ), a dual-mode ratio fluorescence probe was developed to detect and quantify Cu 2+ . The up-conversion ratio fluorescent probe shows a linear range of 0.0500–15.0 μM, with a detection limit as low as 2.76 nM. This method has been successfully applied to detecting Cu 2+ in human serum and has potential applications in biochemical analysis and biological imaging. The successful preparation of up-conversion fluorescent carbon dots without rare earth elements and the ability to perform low-damage detection in high-background biological samples provide a new approach to constructing non-rare earth up-conversion probes.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Fengjuan Ding,Zhanying Chu,Quanqing Zhang,Haiyan Liu,Weibing Zhang
Hydrophilic interaction liquid chromatography is a significant strategy for the separation and enrichment of glycoproteins and glycopeptides . A layered imine-based covalent organic polymer with mesopores (denoted as p-TpBDH) was successfully fabricated by a facile solvothermal method . Then p-TpBDH-OH with abundant hydrophilic groups was evolved from p-TpBDH by the direct reduction. 36 and 40 glycopeptides were identified from IgG digests respectively by p-TpBDH and p-TpBDH-OH. Furthermore, the p-TpBDH-OH exhibits superior selectivity (IgG: BSA = 1: 250) for glycopeptides compared with the p-TpBDH. Encouragingly, a total of 463 glycopeptides assigned to 173 glycoproteins were finally identified from only 2 μL human serum by the p-TpBDH-OH. Compared with p-TpBDH, abundant hydrophilic and nitrogen-containing affinity sites of p-TpBDH-OH facilitate effective hydrophilic interaction between the polymeric material and glycopeptides. All the results demonstrate the functionalized hydrophilic covalent organic polymer has great potential in large-scale N-glycoproteomic research.
研究领域:
生物化学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 13
·3483-3493
·
IF 3.431
Feng Liuxing,Wang Jun,Cui Yanjie,Shi Naijie,Li Haifeng,Li Hongmei
Three reference materials, at relatively low, middle, and high concentrations, were developed for analysis of the mass fractions of electrolytes (K, Ca, Na, Mg, Cl, and Li) in human serum. The reference materials were prepared by adding high purity chloride salts to normal human serum. The concentration range of the three levels is within ±20% of normal human serum. It was shown that 14 units with duplicate analysis is enough to demonstrate the homogeneity of these candidate reference materials. The statistical results also showed no significant trends in both short-term stability test for 1 week at 40 °C and long-term stability test for 14 months. The certification methods of the six elements include isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS), inductively coupled plasma optical emission spectroscopy (ICP-OES), atomic absorption spectroscopy (AAS), ion chromatography (IC), and ion-selective electrode (ISE). The certification methods were validated by international comparisons among a number of national metrology institutes (NMIs). The combined relative standard uncertainties of the property values were estimated by considering the uncertainties of the analytical methods, homogeneity, and stability. The range of the expanded uncertainties of all the elements is from 2.2% to 3.9%. The certified reference materials (CRMs) are primarily intended for use in the calibration and validation of procedures in clinical analysis for the determination of electrolytes in human serum or plasma.
Certified reference materials for K, Ca, Mg, Na, Cl and Li in human serum (GBW09124-09126)
研究领域:
临床检验学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Xiaoxia Zhang,Jiwei Shen,Yinmao Wei,Chaozhan Wang
In this work, a titanium (IV) immobilized covalent organic framework (COF) was developed and used as an efficient adsorbent for highly selective enrichment of nucleotides from human serum. Firstly, an amino-functionalized magnetic COF (Fe 3 O 4 @TpBD(NH 2 ) 2 ) was synthesized by the in situ growth strategy. Alendronate sodium (AS) was then modified on the surface of the Fe 3 O 4 @TpBD(NH 2 ) 2 via Schiff base reaction. Finally, Ti 4+ was chelated to obtain immobilized metal ion affinity chromatography (IMAC) adsorbent (denoted as Fe 3 O 4 @COF-AS-Ti 4+ ). Its adsorption performances toward nucleotides including adenosine triphosphate (ATP), adenosine-5′-diphosphate (ADP) and adenosine-5′-monophosphate (AMP) were investigated, their maximum adsorption capacity were 36.7, 39.0 and 49.6 μmol/g, respectively. The adsorption and desorption can be achieved very fast (in 1 min). In addition, the Fe 3 O 4 @COF-AS-Ti 4+ can still selectively adsorb nucleotides in the presence of other analogues, such as nucleosides, phenols, aromatic amines and phenol amines. It can be served as a good adsorbent for selective enrichment of nucleotides and simultaneous exclusion of biomacromolecules. It has also good synthesis reproducibility and reusability. These features suggest that the Fe 3 O 4 @COF-AS-Ti 4+ nanospheres are of great benefit for pretreatment prior to instrument analysis of nucleotides.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Hazardous Materials Advances
·
2023
·
IF 0
Chunhu Tan,Tingting Zhang,Gongying Wang,Libin Zhang,Chang Liu,Weihua Li,Jiansheng Li,Rui Lu
Currently, despite the wide application of Ti 3 C 2 T x in various fields, little information is available regarding its toxicity to humans, thus, it is necessary to study its interactions with human proteins as well as its biotoxicity. In the study, spectroscopic analysis and molecular simulation were used to investigated the interaction mechanism between human serum albumin (HSA) and Ti 3 C 2 T x with different oxidation degrees. The three prepared Ti 3 C 2 T x materials with different oxidation degrees were analyzed by X-ray diffractometer, X-ray photoelectron spectroscopy, scanning electron microscope and transmission electron microscope, and the results demonstrated that TiO 2 nanoparticles were formed during the oxidation process. In conclusion, the results of the ultra violet spectrum, fluorescence spectra and zeta potential measurements showed that fully oxidized Ti 3 C 2 T x (F-Ti 3 C 2 T x ) had a greater binding ability to HSA than unoxidized Ti 3 C 2 T x (U-Ti 3 C 2 T x ), followed by partially oxidized Ti 3 C 2 T x (P-Ti 3 C 2 T x ). Based on synchronous fluorescence, circular dichroism and infrared characterization, it has been determined that with a gradual increase of Ti 3 C 2 T x oxidation degree, its effect on the conformation of HSA increases, followed by F-Ti 3 C 2 T x > P - Ti 3 C 2 T x > U - Ti 3 C 2 T x . Further analysis of the fluorescence binding ability revealed that hydrogen bonds played a major role in the interaction between HSA and Ti 3 C 2 T x .
研究领域:
生物化学
·
毒理学
·
材料科学
✓ 已验证
DOI
TALANTA
·
2025
·
IF 6.1
Shuojiang Liu,Ying Chen,Xingliang Cheng,Gongke Li,Yuling Hu
Surface-enhanced Raman scattering (SERS) in conjunction with nanozymes has emerged as a powerful technique for bioanalysis due to their signal conversion and amplification effect. However, clinical applications continue to encounter challenges such as inadequate selectivity. Herein, we proposed a novel approach for the selective SERS detection of L-DOPA in human serum with dual enzyme-mimicking MOF-919(Fe–Cu). This material exhibits both catechol oxidase-like and peroxidase-like activities, enabling it to catalyze catecholic compounds to o -quinones and subsequently oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) molecules into SERS-active ox-TMB. Ultrasonic-assisted synthesis ensures uniform and dense loading of AgNPs within MOF-919(Fe–Cu), thereby enhancing SERS activity. A sensitive and selective method for SERS detection of L-DOPA was developed. The catechol oxidase-like activity of MOF-919(Fe–Cu) provides ideal selectivity and anti-interference to L-DOPA, while the cascade catalytic system transforms weak SERS signals of L-DOPA into amplified SERS signals of ox-TMB. The mechanism of dual enzyme-mimicking activity was further discussed. The detection process was optimized to achieve high sensitivity towards L-DOPA with a linear range 0.5–100 μmol/L and low limit of detection (LOD) of 0.16 μmol/L. Furthermore, it has been applied in serum sample of Parkinson's patients, demonstrating excellent recovery and low relative error compared to HPLC-UV. This approach offers an economical, efficient and accurate strategy for the detection of L-DOPA and provides valuable insights for the integration of selective multifunctional nanozyme and sensitive SERS detection method.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Chen Bing,Xu Dafen,Li Zhijun,Jing Yafei,Lin Luping,Li Shaoguang,Huang Liying,Huang Xiuwang,Liu Ailin,Lin Xinhua,Yao Hong
Selaginella doederleinii Hieron is a traditional Chinese medicinal herb widely used to treat different cancers. Previously, we showed that the total bioflavonoid extract of S. doederleinii (TBESD) exhibits anti-carcinogenic activities both in vitro and in vivo. However, the plasma protein binding and pharmacokinetics parameters of TBESD remain unclear. To investigate plasma protein binding, tissue distribution, and excretion of TBESD, rats were administered a single dose of TBESD (600 mg/kg) intragastrically and tissue distribution and excretion of TBESD components were determined by rapid high-performance liquid chromatography and tandem mass spectrometry. TBESD binding to human serum albumin (HSA) was assessed by fluorescence spectroscopy. TBESD components amentoflavone, delicaflavone, robustaflavone, 2″,3″-dihydro-3′,3‴-biapigenin, and 3′,3‴-binaringenin were rapidly absorbed and distributed in various tissues, mostly in the lungs, kidneys, and ovaries, without long-term accumulation. The excretion of bioflavonoids occurred mostly via the intestinal tract and constituted 30% of the administered dose up to 48 h. Spectral analysis indicated that TBESD had a dynamic quenching effect on HSA by binding to one HSA site through hydrophobic interactions and hydrogen bond formation. This is the first comprehensive report on the tissue distribution, excretion, and plasma protein binding of TBESD. This study provides important information on TBESD pharmacokinetics necessary for its further development into a therapeutic form for clinical applications.
研究领域:
药理学
·
中药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）