Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
研究领域:
生物传感技术
·
医学诊断
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Fei Wang,Xin Li,Siyuan Wang,Yitao Cao,Lingqian Zhang,Yang Zhao,Xianzi Dong,Meiling Zheng,Hongyao Liu,Weier Lu,Xinchao Lu,Chengjun Huang
Integrating surface plasmon resonance (SPR) structures on fiber end facets enables the sensing system to be miniature, label-free, real-time, and remote detection. Yet, the wave vector matching condition for SPR excitation is hard to be satisfied by regulating the wave vector of light emitted from fiber ends. Here, we demonstrate a novel SPR microsensor realized by integrating a 3D micro waveguide onto the fiber tip. The mechanism of SPR excitation is illustrated by wave vector matching between the in-plane component of waveguide mode and surface plasmon polaritons . Using two-photon polymerization (2PP) and metallization process, the designed 3D waveguide probe with a length of 10 µm is fabricated on the fiber tip. Then, the microsensor was applied for the immune-sensing of anti-human IgG and human IgG interactions, and a LOD of 1.11 nM was achieved. The ultracompact structure endows with the capability for high-performance detection in the micrometre scale through a convenient “dip-and-read” manner. As a proof-of-concept for this “dip-and-read” small volume sensing, immunoassay detection in a small sample volume of 5 μL was also successfully demonstrated. The obtained results indicated that our 3D fiber-probe SPR microsensor may provide a powerful tool for small-volume biosensing applications.
研究领域:
光学
·
生物传感器
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 12
·5526-5534
·
IF 6.97
Yu-Fang Ma,Li-Juan Wang,Ying-Lin Zhou,Xin-Xiang Zhang
The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis. In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification. TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses. Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment. By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g−1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times). It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples. Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.
研究领域:
分析化学
·
蛋白质组学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 408
·期 4
·1015-1022
·
IF 3.436
Wang Shasha,Chen Zhaopeng,Choo Jaebum,Chen Lingxin
A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3′,5,5′-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H 2 O 2 . Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7–100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3σ/S for UV-vis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics.
A naked-eye ELISA-like immunoassay for determination of human IgG is described based on gold enhanced peroxidase like activity of gold nanoparticles and it is a colorimetric typical sandwich-type immunoassay format with high sensitivity
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ling-Xin Kong,Yan-Xin Zhang,Wei-Gang Zhang,Yun-Shan Zhang,Tie-Yi Yan,Peng-Cheng Geng,Biao Wang
Lab-on-tip technology opens a new door for optical fiber surface plasmon resonance (SPR) sensors. In this paper, we first demonstrate an ultra-compact reflective SPR microtip probe on the flat fiber end in experiment, which has a length of tens of microns and a diameter of several microns. Simulations and experiments show that this new fiber SPR microtip probe coated with a nano-goldfilm has excellent refractive index (RI) sensing characteristic and great potential for biochemical detection at single cell level. Based on the high RI sensitivity, a bio-probe assembled with Chitosan/Polysodium Styrene Sulfonate (Cs/PSS) nano-polyelectrolyte film and the sheep anti-human IgG-HRP is prepared to recognize human IgG specifically. Due to the high surface sensitivity, the SPR microtip probe exhibits an ultra-low detection limit for human IgG. Due to the high bulk sensitivity and high thermo-optical coefficient (TOC) of polydimethylsiloxane (PDMS), a reference temperature probe based on SPR microtip is proposed, which is used to compensate for the temperature cross-sensitivity of the bio-probe. This new SPR microtip probe can be used not only in optical fiber sensor, but also in Tip-Enhanced Near-Field Raman Microscopy and Scanning Near-Field Optical Microscope.
研究领域:
光纤传感器
·
表面等离子体共振(SPR)技术
·
生化传感
·
纳米技术
·
显微镜
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2010
·
IF 0.79
Guo-Feng XU,Hui LI,Cui ZHANG,Zi-Yin SUN,Wen-Ying ZHONG,Dan-Ke XU,Hong-Yuan CHEN
We synthesized and prepared a CdTe quantum dot (QD) probe modified with streptavidin (SA) and successfully established a novel visual detection method based on QD coupled with silver enhancement. Combined with the protein microarray analysis technology, this novel method did well in the detection of the reverse-phase protein microarray. To detect the analytical performance of this method, human IgG was used as a mode in this experiment. Initially, the human IgG was immobilized on aldehyde slides, then the optimized bio-goat anti-human IgG and CdTe-SA probe were added, and finally the silver enhancement coloration was held for 15 min; the results showed a good linear correlation between the signal gray level and the logarithm of human IgG immobilized on slides from 100 pg to 1.6 ng with a correlation coefficient ( R 2 ) of 0.997, while its lowest detectable limit was about 50 pg human IgG. This novel method could carry out the sensitive detection of microamounts of protein and shows the characteristics of a simple method that requires very few instruments, and the results obtained using this method can be easily visualized.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Youming Shen,Youyu Zhang,Meiling Liu,Xiaoyin Liu,Huan Guo,Xiangyang Zhang,Chunxiang Zhang,Haitao Li,Shouzhuo Yao
In this work, a novel sulfur-containing compound with aldehyde groups was synthesized, which was used to immobilize antibodies through the covalent bonding of the aldehyde groups with amino groups, in which no additional chemical cross-linker is required. Human IgG was used as a model analyte to fabricate the electrochemical immunosensor. Using the proposed immunosensor, IgG was detected within the range from 0.01 to 25 ng mL −1 with a detection limit of 0.003 ng mL −1 obtained by 3 S/N. The simple electrochemical immunosensor had a good specificity, stability and reproducibility.
研究领域:
生物化学
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2017
·Vol. 33
·期 4
·481-486
·
IF 1.228
Juan WU,Yanbin JIANG,Lishi WANG,Qiang ZENG
In order to confirm the correlation between interfacial properties of modified surface plasmon resonance (SPR) chips and their SPR responses to immobilized anti-IgG, SPR chips were modified by mercaptoundecanoic acid, poly(ethylene glycol) diacrylate (PEG), PEG-based copolymer and cyclodextrin coupled PEG using self-assembled or radical polymerization methods. The resulting interfacial properties such as film thickness and hydrophilicity were characterized by AFM, elliptic polarization scanners and contact angle meter. Immobilization of human IgG on the modified chips was achieved by EDC/NHS activation through an amide bond. The association between fixed IgG and free anti-IgG was reflected by the variation of SPR responses and the binding ability was evaluated by Langmuir isotherms. As observed, the adsorption between IgG and anti-IgG was affected by the interfacial properties of different modifiers, such that a chip with a thinner and more hydrophilic layer may result in a higher SPR response, producing a larger adsorption equilibrium constant for protein interaction.
研究领域:
生物化学
·
材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Jiaping Guo,Yameng Wang,Shanshan Niu,Hongping Li,Yongmei Tian,Songcheng Yu,Fei Yu,Yongjun Wu,Li-e Liu
The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination of human IgG and then expand it to analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM and IgG, which is of great significance for the screening and diagnosis of COVID-19. In this study, the magnetic Fe3O4 nanospheres coupled with mouse antihuman IgG (Ab1IgG) were used as an immune capture probe (Fe3O4@Ab1IgG) to capture and separate the target, and rabbit antihuman IgG (Ab2IgG) coupled with highly luminescent quantum dot nanobeads (QBs) as a fluorescence detection probe (QBs@Ab2IgG) was used to realize high sensitivity detection. After the formation of a sandwich immunocomplex, the fluorescence intensity of the precipitate after magnetic separation was measured at the excitation wavelength of 370 nm. Under optimal conditions, a wide linear range varying from 0.005 to 40 ng·mL–1 was obtained for the detection of human IgG with a lower limit of detection at 4 pg·mL–1 (S/N = 3). The recoveries of intra- and interassays were 90.0–101.9 and 96.0–106.6%, respectively, and the relative standard deviations were 6.3–10.2 and 2.6–10.5%, respectively. Furthermore, the proposed method was successfully demonstrated to detect human IgG in serum samples, and the detection results were not statistically different (P > 0.05) from commercial enzyme-linked immunosorbent assay kits. This method is sensitive, fast, and accurate, which could be expanded to detect the specific IgM and IgG antibodies against SARS-CoV-2.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
免疫学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Zhen Lei,Jiaxue Gao,Xia Liu,Dianjun Liu,Zhenxin Wang
We developed a three-dimensional (3D) polymer-brush substrate for protein and peptide microarray fabrication, and this substrate was facilely prepared by copolymerization of glycidyl methacrylate (GMA) and 2-hydroxyethyl methacrylate (HEMA) monomers via surface-initiated atom transfer radical polymerization (SI-ATRP) on a glass slide. The performance of obtained poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) (P(GMA-HEMA)) brush substrate was assessed by binding of human IgG with rabbit antihuman IgG antibodies on a protein microarray and by the determination of matrix metalloproteinase (MMP) activities on a peptide microarray. The P(GMA-HEMA) brush substrate exhibited higher immobilization capacities for proteins and peptides than those of a two-dimensional (2D) planar epoxy slide. Furthermore, the sensitivity of the P(GMA-HEMA) brush-based microarray on rabbit antihuman IgG antibody detection was much higher than that of its 2D counterpart. The enzyme activities of MMPs were determined specifically with a low detection limit of 6.0 pg mL–1 for MMP-2 and 5.7 pg mL–1 for MMP-9. By taking advantage of the biocompatibility of PHEMA, the P(GMA-HEMA) brush-based peptide microarray was also employed to evaluate the secretion of MMP-2 and MMP-9 by cells cultured off the chip or directly on the chip, and satisfactory results were obtained.
研究领域:
生物材料
·
生物化学
·
生物技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Lijun You,Baohua Zhong,Ci Huang,Jumei Li,Zhijuan Zheng,Yang Wang
Au coated magnetic polyphosphazene (MPCTP) composite particles ( [email protected] ) were fabricated with sensitive SERS activity. The MPCTP particles were generated by coating polyphosphazene on Fe 3 O 4 nanoparticles through precipitation polycondensation of hexachlorocyclotriphosphazene and phloroglucinol . [email protected] composite particles were obtained by deposition of Au nanoparticles on MPCTP by the reduction of HAuCl 4 . The size and the thickness of the Au shell can be controlled by varying the amount of HAuCl 4 . The magnetic core endowed the composite particles with good magnetic responsiveness, which allowed the analyte to be enriched and separated from the complex matrix, and significantly simplifying the sample pretreatment procedure. The SERS activity of [email protected] composite particles were evaluated by DTNB as model Raman reporter, and the limits of detection (LOD) of DTNB was 10 -8 mol/L. A high efficient SERS immunoassay system based on the [email protected] substrates for the detection of immunoproteins was developed. Human IgG and rabbit IgG were quantitatively determinated simultaneously by this immunoassay system. The quantitative determination of the immunoglobulin G (IgG) was achieved and the LOD of human IgG, rabbit IgG and the mixture of human IgG and rabbit IgG were as low as 10 fg/mL, 100 pg/mL and 1 ng/mL, respectively. The results showed that the [email protected] composite particles have broad application prospects as high performance SERS active substrates for immunoprotein analysis.
研究领域:
材料科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 3
·141
·
IF 5.743
Yijian Huang,Ying Wang,Gaixia Xu,Xing Rao,Jiaxiong Zhang,Xun Wu,Changrui Liao,Yiping Wang
A compact surface plasmon resonance sensor based on an H-shaped optical fiber is proposed and demonstrated. The H-shaped optical fiber was fabricated experimentally by using hydrofluoric acid to controllably corrode the polarization-maintaining fiber. A satisfactory distance between the outer surface of the fiber and the core can be achieved, and then the surface plasmon resonance effect can be excited by coating a metal film of appropriate thickness on the surface of the fiber. This technology can realize the preparation of multiple samples at one time, compared to the traditional side-polishing technique. The H-shaped optical fiber obtained from corrosion exhibits a high surface quality and short lengths, down to only a few hundred microns. The effects of the proposed H-shaped optical fiber on spectral properties are induced by process parameters, including fiber remaining thickness, coating thickness and fiber length, and were investigated in detail. The prepared sensor was used for the specific detection of human IgG, and the minimum human IgG concentration that the sensor can distinguish is 3.4 μg/mL. Such a compact surface plasmon resonance fiber sensor has the advantages of an easy fabrication, good consistency and low cost, and is expected to be applied in the specific detection of biomarkers.Keywords:surface plasmon resonance;biosensor;polarization-maintaining fiber;chemical corrosion
研究领域:
光学传感
·
生物医学工程
·
光纤技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）