BIOTECHNOLOGY LETTERS
·
2024
·
IF 2
Zhao Shuo,Zeng Yu,Li Ying,Wang Zhen,Chen Li,Chen Kequan
This investigation probes the role of the electron mediator, neutral red (NR), in the electrosynthesis process, specifically examining its effect on the production of succinic acid by Actinobacillus succinogenes . Our findings reveal that NR, when integrated into the cell membrane, is pivotal for sustaining MEC efficiency. Nevertheless, it is susceptible to both intrinsic and MECs-induced degradation. Notably, during the exponential growth phase of the bacteria, NR is readily incorporated into the cell membrane. However, the supplemental addition of NR fails to significantly enhance the MEC’s capacity for succinic acid synthesis, no matter what stage of bacterial growth. And significant depletion of membrane-associated NR is not adequately compensated by the NR present in the fermentation liquid. The ORP feedback-regulated MECs adeptly conserve the NR on the cell membrane, which is essential for maintaining the efficiency of long-term electrosynthesis. The presence of NR on the cell membrane is essential for the functionality of MECs, yet its external replenishment hard. Implementing precise electro-potential regulation strategies can effectively diminish the degradation of NR, thus maintaining the system’s efficiency.
研究领域:
微生物学
·
电化学工程
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 20
·9792-9798
·
IF 3.925
Zhiguo Li,Qingpeng Zhu,Zhaofei Liu,Li Sha,Zhiming Chen
Laccase is an important biodegradation agent as the catalytic degradation could be enhanced in the presence of redox mediators. This work aims to improve the removal performance of immobilized laccase for high concentrations of synthetic dye degradation using 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and violuric acid (VLA) as redox mediators. The laccase was immobilized on magnetic hydroxyl-functionalized Fe3O4 nanoparticles (Fe3O4–PEG 2000–Cu2+) based on Cu2+ chelation. The immobilized laccase presented relatively high immobilization capacity and activity retention, improved thermostability and pH tolerance, and excellent reusability. The removal performance assay reveals that the immobilized laccase had high and sustainable removal efficiencies for high concentrations of triphenylmethane dyes in the presence of these redox mediators. It should be mentioned that azo and anthraquinone dyes at concentrations of 25–200 mg L−1 could be rapidly oxidized by the immobilized laccase using these mediators in 20–30 min. A recycling degradation study showed that after 10 catalytic runs, the removal efficiency of triphenylmethane, azo and anthraquinone dyes by the immobilized laccase employing these mediators could still reach 78.3–99.9 (%), 63.1–94.6 (%) and 60.5–74.6 (%), respectively. The outstanding dye degradation performances show that the immobilized laccase has a potential application in wastewater treatment and environmental protection.
研究领域:
环境科学
·
生物技术
·
酶学
✓ 已验证
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Shihai Cao,Ning Zhou,Fenghua Gao,Huan Chen,Fang Jiang
An all-solid-state Z-scheme heterojunction-structured photocatalyst, 3,4-dihydroxybenzaldehyde-functionalized Ga 2 O 3 /graphitic carbon nitride (Ga 2 O 3 -DBD/g-C 3 N 4 ), was synthesized using a facile post-grafting strategy via Schiff base chemistry. It was proposed for the first time that aromatic rings served as electron mediators in the Z-scheme photocatalytic system. In addition, the aromatic rings were conducive to the formation of a well-developed combined interface between Ga 2 O 3 -DBD and g-C 3 N 4 , greatly improving the separation of electrons and holes. The Ga 2 O 3 -DBD/g-C 3 N 4 exhibited a wide absorption range, high charge-separation efficiency and high redox potential, thus enhancing its activity and stability for visible-light photocatalytic nitrogen fixation. The reaction mechanism was demonstrated to be that O 2 was first reduced to H 2 O 2 , which was further oxidized to OH; then, OH reacted with methanol to form CO 2 − , which facilitated the reduction of N 2 to NH 3 . This study demonstrates a simple and cost-effective approach to synthesize all-solid-state Z-scheme photocatalytic system using the aromatic rings, and this system exhibits great potential for practical applications of visible-light photocatalytic nitrogen fixation.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2023
·
IF 9.2
Qingyue Han,Suqing Wang,Wenhao Ren,Fangdan Zhang,Zhanbo Tang,Ju Wang,Haihui Wang
Poly (ethylene oxide) (PEO)-based electrolytes are under intense research because of their high safety, low cost and easy to scale fabrication. But several barriers, such as the poor ionic conductivity , slow reaction kinetics and uneven lithium deposition, still prevent their practical implementation. Herein, ferrocene (Fc) is utilized as a novel redox mediator filler for PEO based-electrolyte to optimize the performance of all-solid-state Li-metal battery . In addition to enhancing the ionic conductivity of electrolyte via Fe–O coordination interaction and Li-cyclopentadienyl ligands cation-π interaction, Fc can also regulate uniform lithium ionic flux over Li metal and inhibit lithium dendrite growth. Moreover, Fc can accelerate the electron transfer and reaction kinetic of LiFePO 4 cathode because of its properties of electron-transfer mediator. As a result, the all-solid-state LiFePO 4 /PEO electrolyte/Li battery with the addition of Fc shows notable cycling stability. It delivers an initial capacity of 158.2 mAh g −1 at 0.5 C and maintains 146.1 mAh g −1 after 300 cycles with a high capacity retention of 92.5%. This work offers a convenient method for durable all-solid-state lithium-metal batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 2.368
Qing Xu,Jianbo Guo,Chunmei Niu,Jing Lian,Zhenghao Hou,Yankai Guo,Shaoying Li
This study investigated the denitrification characteristics and practical applications of PET–CAs (chloroanthraquinones immobilised on polyethylene terephthalate), which are novel functional biocarriers. The PET–CAs were characterized using Fourier transform infrared spectroscopy (FT-IR) and energy dispersive spectrometry (EDS). Among the PET–CAs, PET-1,8-DCA (PET modified by 1,8-dichloroanthraquinone) was the best functional biocarrier for accelerating the denitrification process with approximately 0.14 mmol anthraquinone. The denitrification process was pseudo-zero order, with C NO3 − = 175.64–19.28 t and R 2 = 0.9715. The pH of the PET-1,8-DCA system increased and ultimately reached 9.7. The stabilized ORP value with PET-1,8-DCA was approximately 70 mV lower than that of the control with PET . This study explored a novel design concept for the immobilization of quinone.
研究领域:
环境生物学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Chemistry
·
2015
·Vol. 2015
·期 1
·670194
·
IF 0.772
Youxun Liu,Mingyang Yan,Yuanyuan Geng,Juan Huang
Efficient reuse and regeneration of spent mediators are highly desired for many of the laccases’ biotechnology applications. This investigation demonstrates that a redox mediator 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) covalently attached to silica nanoparticles (SNPs) effectively mediated dye decolorization catalyzed by laccase. Characteristics of ABTS-modified silica nanoparticles (ABTS-SNPs) were researched by scanning electron microscopy and Fourier-transformed infrared spectroscopy. When ABTS and ABTS-SNPs were used as laccase mediators, the decolorization yields of 96 and 95% were, respectively, obtained for indigo carmine dye. The results suggest that ABTS immobilized on SNPs can be used as laccase mediators as they retain almost the same efficiency as the free ABTS. The oxidized ABTS-SNPs were regenerated by their reduction reaction with ascorbic acid. Decolorization efficiency of regenerated ABTS-SNPs and their initial forms were found to be almost equivalent. Six reuse cycles for spent ABTS-SNPs were run for the treatment of indigo carmine, providing decolorization yields of 96–77%. Compared with free mediator, the immobilized mediators have the advantage of being easily recovered, regenerated, and reused making the whole process environmentally friendly.
研究领域:
环境生物技术
·
纳米生物技术
·
酶工程
·
污染物降解
·
绿色化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2024
·
IF 4.1
Liwen Zhang,Luyao Xi,Shuyu He,Huizi Wen,Shanyi Tan,Shaohua Chen
Bioremediation has garnered considerable interest due to its advantages of economy and no secondary pollution. However, the direct electron transfer rate between microorganisms and Cr(VI) is low. The bioreduction of Cr(VI) by Shewanella oneidensis MR-1 ( S. oneidensis ) in the presence of formylmethylflavin (FMF) was conducted to understand how FMF mediated the extracellular electron transfer process to improve Cr(VI) removal. In this study, FMF was firstly synthesized by modifing RF to increase its solubility and redox activity. The findings indicate that S. oneidensis /FMF exhibited better Cr(VI) removal performance compared to that of S. oneidensis . Under optimum conditions, 40 mg/L Cr(VI) could be completely removed by S. oneidensis /FMF within 120 h, while only 48.6% of Cr(VI) was removed by S. oneidensis , and the first order rate constant (k) for the Cr(VI) elimination by S. oneidensis /FMF (0.033 h −1 ) was about 4.1-fold greater than that of S. oneidensis (0.008 h −1 ). Moreover, the removal of Cr(VI) by S. oneidensis /FMF were dominated by reduction, and supplemented by adsorption and complexation. FMF enhance the extracellular electron transfer rate (EETR) was confirmed by electrochemical cyclic voltammetry (CV) and linear scanning voltammetry (LSV) experiments. This study emphasizes the potential important role of FMF in environmental bioremediation.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Dong Ma,Juan Wu,Mengchun Gao,Yanjun Xin,Yuying Sun,Tianjin Ma
The electron mediator plays an important role in charge transfer in an artificial all-solid-state Z-scheme system. According to the energy band theory, a visible light photocatalytic system was designed by using reduced graphene oxide (RGO) as the electron mediator. The as-prepared g -C 3 N 4 /RGO/Bi 2 MoO 6 composite was synthesized by hydrothermal process, and it was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), nitrogen sorption , Raman spectroscopy , Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS) and diffuse reflectance spectroscopy (DRS). The photocatalytic activity of the g -C 3 N 4 /RGO/Bi 2 MoO 6 composite was evaluated by analyzing the degradation of Rhodamine B (Rh B) under visible light irradiation. The photocatalytic degradation rate of Rh B for the g -C 3 N 4 /RGO/Bi 2 MoO 6 composite was 8.73 and 5.19 times for the Bi 2 MoO 6 and g -C 3 N 4 /Bi 2 MoO 6 composite, respectively. The matched Fermi level structure provided the favorable interfacial charge transfer from the Bi 2 MoO 6 Photosystem II (PS II) to the g -C 3 N 4 Photosystem I (PS I) to promote the photocatalytic degradation of Rh B, suggesting that RGO was a suitable electron mediator in the Z-scheme system. The present study can provide a scientific basis for the rational design and realization of the Z-scheme system in environmental application.
研究领域:
材料科学
·
环境科学
·
光催化技术
✓ 已验证
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 12
·1955-1966
·
IF 3.434
Liu Runtang,Wang Silin,Han Mengyao,Zhang Wei,Xu Huajin,Hu Yi
In this study, an amino-functionalized ionic liquid-modified magnetic chitosan (MACS-NIL) containing 2,2-diamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) was used as a carrier, and dialdehyde starch (DAS) was used as a cross-linking agent to covalently immobilize laccase (MACS-NIL-DAS-lac), which realized the co-immobilization of laccase and ABTS. The carrier was characterized by Fourier infrared transform spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction analysis, electron paramagnetic resonance, etc. The immobilization efficiency and activity retention of MACS-NIL-DAS-lac could reach 76.7% and 69.8%, respectively. At the same time, its pH stability, thermal stability, and storage stability had been significantly improved. In the organic pollutant removal performance test, the removal rate of 2,4-dichlorophenol (10 mg/L) by MACS-NIL-DAS-lac (1 U) could reach 100% within 6 h, and the removal efficiency could still reach 88.6% after six catalytic runs. In addition, MACS-NIL-DAS-lac also showed excellent degradation ability for other conventional phenolic pollutants and polycyclic aromatic hydrocarbons. The research results showed that MACS-NIL-DAS fabricated by the combination inorganic material, organic biomacromolecules, ionic liquid, and electron mediator could be used as a novel carrier for laccase immobilization and the immobilized laccase showed excellent removal efficiency for organic pollutants.
研究领域:
生物技术
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Peng Zhang,Peng Zhou,Jiali Peng,Yang Liu,Heng Zhang,Chuanshu He,Zhaokun Xiong,Wen Liu,Bo Lai
Reports that the exploitation of metal-free carbon materials to enhance permanganate (PM) oxidation to abate organic pollution in water have emerged in recent publications. However, the activation mechanism and active sites involved are ambiguous because of the intricate physicochemical properties of carbon. In this study, reduced graphene oxide (rGO) as a typical carbon material exhibits excellent capability to boost permanganate oxidation for removing a wide array of organic contaminants. The simultaneous two reaction pathways in the rGO/PM system were justified: i) rGO donates to electrons to decompose PM and produce highly reactive intermediate Mn species for oxidizing organic contaminants; ii) rGO mediates electron transfer from organics to PM. Oxygen-containing groups (hydroxyl, carboxyl, and carbonyl) were justified as electron-donating groups, while structural defects (vacancy and edge defects) were shown to be critical for rGO-mediated electron transfer. Therefore, the oxidation pathway of the rGO/PM system can be controlled by regulating oxygen functional groups and structural defects. The changeover from electron donor to electron mediator by decorating surface active sites of carbon materials will be of great help to the design and application of carbocatalysts.
研究领域:
环境科学
·
催化材料
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Li-Na Song,Li-Jun Zheng,Xiao-Xue Wang,Yi-Feng Wang,Yue Wang,Shuang Liang,Ji-Jing Xu
The design of highly efficient electrocatalysts is a promising strategy to improve the electrochemical kinetics of Li–CO2 batteries. However, electrocatalysts usually aim to reduce the energetic barrier for the corresponding electrochemical reactions; little attention has been given to modulating the kinetics that directly determine the local concentration of reaction molecules surrounding catalysts. Herein, we present a systematic study on the role of Li+ reunion on the improvement of reaction kinetics in Li–CO2 batteries with a Cu cone cathode. Specifically, this local, geometry-driven tip effect can enrich the local electron concentration to facilitate Li+ ions diffusion from the bulk electrolyte to the surface of catalyst, leading to boosted catalytic performance. Further studies demonstrate that Cu(II/I) as a solid redox mediator dominates the reversible bulk redox reactions in a Cu cone cathode, which acts as an electron–hole transfer agent and permits the efficient reduction and oxidation of solid Li2CO3, contributing to an accessible theoretical discharge voltage, low charge potential below 3.2 V, impressive rate capability, and a long cycling stability (333 days) for Li–CO2 batteries. The exploitation of the sharp-tip enhancement effect and dynamic creation of catalytic active sites is expected to become routine practice in future mechanistic studies for metal-air batteries.
研究领域:
电化学
·
材料科学
✓ 已验证
DOI
高影响因子
Biosensors-Basel
·
2024
·Vol. 15
·期 1
·9
·
IF 4.9
Changyun Quan,Yue Zhang,Yuanyuan Liu,Liping Wen,Haixia Yang,Xueqin Huang,Minghui Yang,Binjie Xu
Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 µmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm2). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）