Frontiers in Endocrinology
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2024
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IF 3.9
Zhu Hanyi,Wu Ming,Mou Junjie,Yang Xueqi,Xu Qian,Zhang Yongjian,Zhang Hao,Wang Xinran,Xue Huiliang,Xu Jinhui,Chen Lei,Xu Laixiang
The circadian clock regulates the behavior, physiology, and metabolism of mammals, and these characteristics, such as sleep-wake cycles, exercise capacity, and hormone levels, exhibit circadian rhythms. Light signaling is the main stimulator of the mammalian circadian system. The photoperiod regulates the reproductive cycle of seasonal breeding animals, and the circadian clock plays a pivotal role in this process. However, the role of the clock in coordinating animal behavior and physiology in response to photoperiodic changes needs further investigation. The present study investigated the changes and correlation of behavioral activities, physiological indicators, and gene expression in female striped hamsters (Cricetulus barabensis) within 24 h under a 12L:12D photoperiod. We found that the daily rhythms of sleep-wake and open field were significant in hamsters. The expression of clock genes, melatonin receptor genes, and genes involved in general metabolism oscillated significantly in central and peripheral tissues (brain, hypothalamus, liver, ovary, and thymus) and was significantly associated with behavior and physiology. Our results revealed that the neuroendocrine system regulated the rhythmicity of behavior and physiology, and central and peripheral clock genes (Bmal1, Clock, Per1, Per2, Cry1, and Cry2), melatonin receptor genes (MT1, MT2, and GPR50), and metabolizing genes (SIRT1, FGF21, and PPARα) played important roles. Our results suggest that central and peripheral circadian clocks, melatonin receptors, and genes involved in general metabolism may play key roles in maintaining circadian behavior and metabolic homeostasis in striped hamsters. Our results may have important implication for rodent pest control.
研究领域:
动物学
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分子生物学
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内分泌学
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DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2018
·Vol. 62
·期 24
·1800728
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IF 5.151
Chun-Feng Guo,Shuang Zhang,Ya-Hong Yuan,Jing-Yan Li,Tian-Li Yue
Scope Lactobacillus casei F0822-fermented milk has exhibited significant hypocholesterolemic activity in hamsters in the previous study. Under this premise, the objective of this study is to further explore whether bile salt hydrolase (BSH) and S-layer protein (SLP) of the strain have a significant influence on hypocholesterolemic activity of the fermented milk. Methods and results Independent and double interposon mutants of BSH and SLP genes are constructed from wild-type L. casei F0822 via chromosomal insertion of chloramphenicol or/and erythromycin resistance genes based on double-crossover homologous recombination. The mutants- and the wild-type strain-fermented milk is prepared (viable counts of approximately 8.0 × 10 8 colony-forming units mL –1 each) and intragastrically administered to high-cholesterol-fed hamsters once daily at a dose of 1.25 mL d –1 for 28 d, respectively. Both the BSH-deficient mutant- and the SLP-deficient mutant-fermented milk significantly ( p < 0.05) increase serum total and LDL-cholesterol levels in hamsters compared with the wild-type strain-fermented milk. However, only the BSH-deficient mutant-fermented milk could significantly ( p < 0.05) increase hepatic total and esterified cholesterol levels in hamsters. Conclusion Both BSH and SLP have a significant influence on the hypocholesterolemic activity of L. casei F0822-fermented milk in hamsters. Nevertheless, the BSH is greater than the SLP in this regard.
研究领域:
微生物学
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营养学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2018
·Vol. 62
·期 16
·1800170
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IF 5.151
Chun-Feng Guo,Ya-Hong Yuan,Tian-Li Yue,Jing-Yan Li
Scope Rats and hamsters are the most commonly used animal models for evaluating the hypocholesterolemic activity of potential probiotic strains, whereas little or no information has been reported on whether the animal models would affect the experimental conclusions regarding the hypocholesterolemic efficacy of the strains. Methods and results Both high-cholesterol-fed rats and hamsters were intragastrically administered viable cells of bile salt hydrolase-active Lactobacillus acidophilus K16 once daily (1 × 10 10 CFU per kg body weight) for 28 d. It was found that the strain did not significantly ( p > 0.05) affect the serum and hepatic cholesterol levels in rats, whereas it significantly decreased ( p < 0.01 or p < 0.001) the serum total and non-HDL-cholesterol as well as hepatic-free, esterified, and total cholesterol levels in hamsters by 29.6%, 38.8%, 15.8%, 36.2%, and 34.0%, respectively. Conclusion These data suggest that the hypocholesterolemic efficacy of L. acidophilus K16 is substantially different between high-cholesterol-fed hamsters and rats and that hamsters are a better model system than rats for evaluating the hypocholesterolemic efficacy of potential probiotic strains due to their similarity to humans in biliary bile acid composition, including types of bile acids and their conjugation form.
研究领域:
营养学
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微生物学
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动物模型
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DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
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2025
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IF 5.6
Shuaibing Liu,Jinxin Miao,Hang Li,Guangbo Ge,Quanjun Yang,Songfeng Zhao,Cheng Guo
Primary sclerosing cholangitis (PSC) is a chronic liver disease characterized by bile duct inflammation and fibrosis, ultimately leading to liver dysfunction. The role of peroxisome proliferator-activated receptor α (pparα) in regulating hepatic drug-metabolizing enzymes, including cytochrome P450s (P450s) and UDP-glucuronosyltransferases (UGTs), in PSC remains insufficiently understood. In this study, wild-type and pparα −/− Syrian hamsters were used to establish a PSC model, aiming to investigate the effects of pparα on the expression and activity of P450 and UGT enzymes. Sequential windowed acquisition of all theoretical fragment ions profiling and multiple reaction monitoring mass spectrometry with high resolution assays revealed a significant downregulation of CYP2C27 and CYP2D20 in pparα−/− hamsters. Enzymatic kinetic assays demonstrated reduced activity for several enzymes, including CYP1A2 (involved in the metabolism of acetaminophen), CYP2C family (responsible for 4′-hydroxy diclofenac formation), CYP2D family (involved in dextrorphan production), CYP2E1 (involved in 6-hydroxy chlorzoxazone production), and UGTs (responsible for chenodeoxycholic acid 24-acyl-β-D-glucuronide and mycophenolic acid glucuronide formation). These reductions suggest impaired drug metabolism and an increased risk of drug-drug interactions in conditions of reduced pparα function. In contrast, enzymes such as the CYP2B family, CYP3A family, and UGTs involved in N-Acetyl serotonin β-D-glucuronide formation retained their activity, indicating selective pathway preservation in pparα −/− hamsters. These findings highlight the selective regulatory roles of pparα on certain drug-metabolizing enzymes, providing important insights into metabolic regulation in pparα-deficient states.
研究领域:
肝脏病学
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分子药理学
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药物代谢
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毒理学
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代谢调控
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实验动物模型
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转录调控
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL RHYTHM RESEARCH
·
2023
·
IF 1.1
Xing-Chen Wang,Zhe Wang,Chuan-Li Wang,Hui-Liang Xue,Ming Wu,Lei Chen,Chao Fan,Jin-Hui Xu,Lai-Xiang Xu
Changes in photoperiod affect both oxidative stress (OS) levels and antioxidant enzyme activities in animals. The liver accounts for a vital body metabolic organ. Different light durations may produce different outcomes in terms of OS and liver antioxidant pathways, but the underlying mechanism is not yet understood. This study investigated the effects of diverse photoperiods (SD, short day; MD, moderate day; LD, long day) on OS degree (H2O2 and MDA), antioxidant enzyme activities (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), liver total antioxidant capacity, and the Nrf2-Keap1 signaling pathway in striped dwarf hamsters (Cricetulus barabensis) livers. Our results showed that (1) SD treatment for 8 weeks increased the levels of phosphorylated Nrf2, upregulated the Nrf2-Keap1 signaling pathway, and increased the protein expression of SOD and GPx, while decreasing the MDA concentration, all of which reduced the OS degree. (2) Additionally, LD treatment for 8 weeks reduced the extent of Nrf2 phosphorylation, resulting in downregulation of the Nrf2-Keap1 pathway, thus reducing protein expression of SOD2 and CAT, increasing the concentrations of H2O2 and MDA, and increasing OS degree. Collectively, OS levels in C. barabensis liver decreased during SD but increased during LD.
研究领域:
生理学
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分子生物学
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生物化学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2023
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IF 11.4
Guangrong Sun,Pei Guo,Han Yeong Kaw,Meijiao Zhou,Wei Wang
Constant efforts have been devoted to exploring new disinfection byproducts in drinking water causally related to adverse health outcomes. In this study, five halogenated nucleobases were identified as emerging disinfection byproducts in drinking water, including 5-chlorouracil, 6-chlorouracil, 2-chloroadenine, 6-chloroguanine, and 5-bromouracil. We developed a solid phase extraction–ultraperformance liquid chromatography–tandem mass spectrometry method with the limits of detection (LOD) and recoveries ranging between 0.04–0.86 ng/L and 54–93%, respectively. The detection frequency of the five halogenated nucleobases ranged from 73 to 100% with a maximum concentration of up to 65.3 ng/L in the representative drinking water samples. The cytotoxicity of the five identified halogenated nucleobases in Chinese hamster ovary (CHO-K1) cells varied with great disparity, in which the cytotoxicity of 2-chloroadenine (IC50 = 9.4 μM) is appropriately three times higher than emerging DBP 2,6-dichloro-1,4-benzoquinone (IC50 = 42.4 μM), indicating the significant toxicological risk of halogenated nucleobase-DBPs. To the best of our knowledge, this study reports the analytical method, occurrence, and toxicity of halogenated nucleobase-DBPs for the first time. These findings will provide a theoretical basis for further research on probing the relationship between its mutagenicity and human health risk.
研究领域:
环境科学
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毒理学
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分析化学
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水处理
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DOI
PubMed
高影响因子
Environmental Science & Technology Letters
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2018
·
IF 5.869
Di Zhang,Wenhai Chu,Yun Yu,Stuart W. Krasner,Yang Pan,Jun Shi,Daqiang Yin,Naiyun Gao
2-Chlorophenylacetonitrile (2-CPAN) and 3,4-dichlorophenylacetonitrile (3,4-DCPAN), representatives of a new class of nitrogenous aromatic disinfection byproducts, were first identified and quantified in chlorinated and chloraminated drinking waters. The impacts of pH, disinfectant residuals, and quenching agents on the stability of two chlorophenylacetonitriles (CPANs) were investigated. The two CPANs slightly degraded with increasing pH (5–9) and chlorination doses (0–4 mg/L) after 5 days, and NH2Cl did not cause degradation of CPANs. Among the commonly used quenching agents, the reaction between sodium sulfite and CPANs was negligible, whereas the others reduced CPANs by varying extents after 7 days. Notably, the two CPANs in finished water collected from seven drinking water treatment plants were quantified. 2-CPAN was detected between 170 and 530 ng/L with a median concentration of 220 ng/L, whereas 3,4-DCPAN ranged from below method detection limit (100 ng/L) up to 320 ng/L with a median concentration of 130 ng/L. Moreover, cytotoxicity of the CPANs and their aliphatic counterparts was determined using Chinese hamster ovary cells. The LC50 values are 133, 83, 436, 260, 905, 4150, and 8900μM for 2-CPAN, 3,4-DCPAN, chloroacetonitrile, dichloroacetonitrile, chloroacetic acid, dichloroacetic acids, and trichloromethane, respectively. Because of the relatively high stability and high toxic potencies of CPANs, the occurrence of CPANs in drinking water deserves attention.
研究领域:
环境科学
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水处理
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毒理学
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DOI
高影响因子
Carbohydrate Polymer Technologies and Applications
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2024
·
IF 6.2
Chang Liu,Chao He,Qing Zeng,Dengqun Liao,Xianen Li
Urolithin B (UB) has significant health benefits, including a hypolipidemic effect, but its poor water solubility limits its bioavailability and pharmacological activities. In order to increase UB's water solubility, we used cyclodextrin complexation to prepare UB–methyl-β-cyclodextrin inclusion complex (UB–M-β-CD). Our result showed that water solubility of UB in inclusion complex was about 0.3 µg/mL, which was increased by 3875 folds than pure UB. In-vivo pharmacokinetic studies showed the enhanced UB content in rat plasma and the improved bioavailability with UB–M-β-CD. In hyperlipidemic hamsters, UB–M-β-CD reduced body weight and organ fat accumulation more effectively than UB alone. Both UB and UB–M-β-CD decreased serum lipid levels except that of high-density lipoprotein cholesterol. Meantime, UB–M-β-CD reversed hepatic bile acid dysregulation better than UB. Further comprehensive analysis revealed that UB–M-β-CD had the superior effects on hyperlipidemia, suggesting that cyclodextrin complexation enhances the therapeutic potential of UB in modulating liver lipid metabolism. Our results indicate that UB–M-β-CD has the great potentials in treatment of hyperlipidemia and related metabolic disorders.
研究领域:
药理学
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药物化学
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代谢性疾病
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DOI
高影响因子
TOXICOLOGY LETTERS
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2014
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IF 3.355
Zhangjian Chen,Yun Wang,Te Ba,Yang Li,Ji Pu,Tian Chen,Yanshuang Song,Yongen Gu,Qin Qian,Jinglin Yang,Guang Jia
With the extensive application of titanium dioxide (TiO 2 ) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO 2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO 2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO 2 NPs (75 ± 15 nm) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The γ-H 2 AX assay showed TiO 2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO 2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO 2 NPs at the dose of 0, 5, 10, 20, 50 and 100 μg/mL. Significant decreases in cell viability were detected in all the treated groups after 24 h and 48 h exposure. Significant DNA damage was only observed at the concentration of 100 μg/mL after 24 h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 μg/mL after 2 h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO 2 NPs, and TiO 2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO 2 NPs induced genotoxic effects.
研究领域:
毒理学
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2023
·
IF 4.9
Xiaojun Hu,Renhan Dong,Sheng Huang,Yisheng Zeng,Wei Zhan,Xiaofang Gao,Dong Tian,Jian Peng,Jiewei Xu,Ting Wang,Yaying Zhang,Xiaohui Wang,Xiaoxia Zhang,Jin Liu,Bing Guang,Tai Yang
Clostridioides difficile infection (CDI) causes severe diarrhea and colitis, leading to significant morbidity, mortality, and high medical costs worldwide. Oral vancomycin, a first-line treatment for CDI, is associated with a high risk of recurrence, necessitating novel therapies for primary and recurrent CDI. A novel small-molecule compound, CDBN-YGXZ, was synthesized by modifying the benzene ring of nitazoxanide with lauric acid. The mechanism of action of CDBN-YGXZ was validated using a pyruvate:ferredoxin/flavodoxin oxidoreductase (PFOR) inhibition assay. The efficacy of CDBN-YGXZ was evaluated using the MIC test and CDI infection model in mice and hamsters. Furthermore, metagenomics was used to reveal the underlying reasons for the effective reduction or prevention of CDI after CDBN-YGXZ treatment. The inhibitory activity against PFOR induced by CDBN-YGXZ. MIC tests showed that the in vitro activity of CDBN-YGXZ against C. difficile ranging from 0.1 to 1.5 μg/mL. In the mouse and hamster CDI models, CDBN-YGXZ provided protection during both treatment and relapse, while vancomycin treatment resulted in severe relapse and significant clinical scores. Compared with global effects on the indigenous gut microbiota induced by vancomycin, CDBN-YGXZ treatment had a mild influence on gut microbes, thus resulting in the disappearance or reduction of CDI recurrence. CDBN-YGXZ displayed potent activity against C. difficile in vitro and in vivo, reducing or preventing relapse in infected animals, which could merit further development as a potential drug candidate for treating CDI.
研究领域:
药理学
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传染病学
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微生物学
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药物开发
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Wenjun Wang,Chunlong Liu,Xuemei Zhang,Jun Yan,Jiaxing Zhang,Shengping You,Rongxin Su,Wei Qi
Invasive Aspergillosis is a high-risk illness with a high death rate in immunocompromised people due to a lack of early detection and timely treatment. Based on immunology study, we achieved an efficient production of anti-galactomannan antibody by Chinese hamster ovary (CHO) cells and applied it to time-resolved fluoroimmunoassay for Aspergillus galactomannan detection. We first introduced dual promoter expression vector into CHO host cells, and then applied a two-step screening strategy to screen the stable cell line by methionine sulfoximine pressurization. After amplification and fermentation, antibody yield reached 4500 mg/L. Then we conjugated the antibodies with fluorescent microspheres to establish a double antibody sandwich time-resolved fluoroimmunoassay, which was compared with the commercial Platelia™ Aspergillus Ag by clinical serum samples. The preformed assay could obtain the results in less than 25 min, with a limit of detection for galactomannan of approximately 1 ng/mL. Clinical results of the two methods showed that the overall percent agreement was 97.7% (95% CI: 96.6%–98.4%) and Cohen's kappa coefficient was 0.94. Overall, the assay is highly consistent with commercial detection, providing a more sensitive and effective method for the rapid diagnosis of invasive aspergillosis.
研究领域:
免疫学
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医学微生物学
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分子生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2020
·Vol. 43
·期 19
·3816-3823
·
IF 2.878
Yu-Ming Fang,Hong-Yun Zhu,Dong-Qiang Lin,Shan-Jing Yao
Short peptide biomimetic affinity chromatography as a novel antibody separation chromatography is a potential alternative to protein A chromatography. However, if directly attaching ligand to matrix, the adsorption capacity and mass transfer rate would be affected by pore blockage and steric effect. Grafting resin is an effective method to solve this problem by using polymer as a bridge between matrix and ligand. In this work, a novel resin was prepared by grafting a tetrapeptide to the dextran-grafted matrix. Then, the adsorption properties for human immunoglobin G and BSA were determined. The results showed the saturation adsorption capacity could reach to 133 mg/g resin at pH 8.9 with a significantly low dissociation constant (0.03 mg/mL). The influence of flow rates to dynamic binding capacity of this resin was less than that of the non-grafted resin. The separation performance of the resin showed monoclonal antibody could be well isolated from the Chinese hamster ovary culture supernatant at pH 9.0 with the purity of 93.0% and yield of 84.7% by one step. Overall, this resin could achieve higher binding capacity by the possible of gaining higher ligand density, indicating its potential significance for separation in larger scale systems.
研究领域:
生物化学
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生物技术
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药学
·
化工
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）