MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Meng-ling Peng,Ying-ying Zhan,Li-fen Cao,Anastassiya V. Gadetskaya,Kai-jing Yu,Jing Zhang,Xiao-hui Qiu,Zhi-hai Huang,Wen Xu
Naturally aged GCP (peel of Citrus reticulata ‘ChaChi’) is a traditional medicine and food ingredient in China. Extensive studies have explored how the aging process affects the chemical and biological properties. In the present study, we used a rigorous sample collection protocol in which GCP were annually collected from a single citrus orchard and aged for 1 to 6 years in a sample warehouse for natural aging. A global chemical investigation was conducted using GC–MS and UHPLC-Orbitrap-MS, identifying 37 volatile and 112 non-volatile chemicals. Volatile terpenes and flavonoid glycosides metabolized into other constituents during aging via oxidation and hydrolysis. The contents of 55 major targets and pseudotargeted metabolomics analysis revealed distinct variation trends in polymethoxyflavones (PMFs) and limonoids. Antioxidant assays of volatile and non-volatile extracts indicated that four-year GCP samples exhibit the best antioxidant activity. The accumulation of terpenoid alcohols and sesquiterpenes, but not terpenes, presented significant positive correlations with antioxidant activity. For non-volatile constituents, PMFs and limonoids displayed significant positive correlations with antioxidant activity. RAW 264.7 cell test showed that all samples demonstrated moderate anti-inflammatory effects, and total PMFs correlated positively with anti-inflammatory activity. This study enhances understanding of the aging mechanism of GCP and establishes a rationale for its practical application.
研究领域:
植物化学
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药理学
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食品科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2016
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IF 4.839
Dezhen Wang,Xinru Wang,Ping Zhang,Yao Wang,Renke Zhang,Jin Yan,Zhiqiang Zhou,Wentao Zhu
Epidemiological studies indicate that exposure to persistent organic pollutants is positively associated with the prevalence of obesity. To delineate the potential role of technical-grade chlordane in obesity development, chlordane metabolism and chlordane-induced metabolic changes were investigated in mice fed high-fat diet (HFD) over a 6-week period. Gas chromatography–electron capture detector analysis showed that HFD induced more accumulation of technical chlordane in the liver, muscle and adipose tissue . The enantioselectivities of oxychlordane in selected tissues were also influenced by HFD. 1 H NMR-based liver metabolome indicated that technical chlordane can enhance the metabolic alterations induced by HFD. Compared with the low-fat diet (LFD) group, no differences were observed in the LFD + chlordane group. However, as many as 16 metabolites were significantly different between the HFD group and HFD + chlordane group. Moreover, compared to the LFD + chlordane group, the abundances of 24 metabolites significantly increased or decreased in the HFD + chlordane group. Twenty metabolites were altered in the HFD group compared to the LFD group. Tryptophan profiling suggested that both chlordane and HFD can disturb tryptophan catabolism. These interactions between technical chlordane and HFD suggest that technical chlordane is a candidate obesogen.
研究领域:
环境科学
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营养学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Chinese Herbal Medicines
·
2025
·
IF 8.9
Weiwei Jiang,Xiaoyun Yan,Chenfei Lu,Xiaowei Xu,Enting Xu,Dandan Chen,Yuqiu Huang,Qingsong Shao,Aimin Lv
Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant. The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A. roxburghii , and to assess the effects of exogenous polyamines (PAs) on bud differentiation and metabolism, thereby providing a theoretical basis for understanding the flowering form and physiology of A. roxburghii . Methods In this study, morphological and biochemical responses in flower development stages of A. roxburghii were investigated using paraffin sections and stereomicroscope. A. roxburghii was divided into five periods, including vegetative growth period, flower bud period, flowering period, late flowering period and fruiting period. During the flowering phase, specific biochemical parameters were measured, including soluble sugar content, superoxide dismutase (SOD) activity, soluble protein content, peroxidase (POD) activity, and catalase (CAT) activity. These measurements were conducted to understand the biochemical changes occurring within A. roxburghii during its flowering process. Furthermore, the effects of PAs on bud differentiation were examined. Additionally, the activities of S-adenosylmethionine decarboxylase (SAMDC) and polyamine oxidase (PAO), as well as the content of polyphenols, polysaccharides, and flavonoids in A. roxburghii , were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs. Results During the flowering phase of A. roxburghii , soluble sugar content and SOD activity were steadily declining. Soluble protein content was initially increasing and then reducing, and POD and CAT activities showed opposite pattern. In addition, the effects of exogenous PAs on bud differentiation were investigated. Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A. roxburghii and advanced the flowering. The activities of SAMDC, PAO, and the content of polyphenols, polysaccharides and flavonoids in A. roxburghii significantly increased after PA treatment, demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content. Conclusion The flower development of A. roxburghii was divided into five stages, with significant changes in soluble sugar, protein, POD, SOD, CAT, MDA, and PRO levels. Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering, increasing SAMDC and PAO activities, suggesting accelerated PA metabolism. PAs also improved active component content. These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A. roxburghii .
研究领域:
植物学
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药用植物学
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生理学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2021
·Vol. 105
·期 20
·7997-8007
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IF 4.813
Shu Lili,Zeng Zhiheng,Dai Jianqing,Cheng Yi,Lu Yuanping,Chen Meiyuan,Zeng Hui
Agaricus bisporus is the most widely cultivated edible mushroom in the world. Strain quality has an important influence on the yield of A. bisporus , with strains that exhibit aging being a common problem during cultivation. However, little is known about the aging mechanisms of A. bisporus strain. In this study, the normal A. bisporus As2796 strain was compared to the aging A. bisporus As2796Y strain (which was previously discovered during cultivation). In the aging As2796Y mycelia, the mycelial growth rate and fruiting body yield were decreased and the chitin level and cell wall thickness were increased. Additionally, intracellular vacuoles increased, there was cytoplasmic shrinkage, and the sterol level which stabilizes the cell membrane decreased, which led to cytoplasmic outflow and the exudation of a large amount of yellow water from the mycelia. Additionally, there was increased electrolyte leakage. Furthermore, gas chromatography-mass spectrometry (GC/MS) was used to profile the metabolic changes in the aging As2796Y mycelia compared to the normal As2796 mycelia. A total of 52 differential metabolites were identified (75% were downregulated and 25% were upregulated in As2796Y). The reduction of many metabolites decreased the mycelial viability and the ability to maintain cell stability. Overall, this study is the first to report on the morphologic and metabolic changes in aged A. bisporus mycelia, which will aid future research on the mechanisms underlying A. bisporus mycelial aging.
Key points
• Aging of Agaricus bisporus strains will greatly reduce the fruiting body yield. • Aging of Agaricus bisporus strains can significantly change the cell structure of mycelia. • Many metabolites in the mycelium of aging spawn As2796Y significantly changed.
研究领域:
真菌学
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代谢组学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
Foods
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2025
·Vol. 14
·期 1
·94
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IF 5.1
Yaxun Qiao,Wenjie Yu,Keju Li,Jingze Cao,Jie Zhu,Qiuning Wang,Jiaqi Zhao,Yunping Wang,Liping Luo,Jinwang Li,Fangjian Ning
The ‘Jingbaili’ pear is a national geographical indication product of China, featuring an oblate shape and being rich in nutrients. But the quality of the ‘Jingbaili’ pear is unstable. Xenia can cause changes in the quality of pears, but the effect of xenia on the ‘Jingbaili’ pear is unknown, and its mechanism is still unclear. In order to clarify the effect of pollination on the fruit quality of the ’Jingbaili’ pear, this research pollinated ‘Jingbaili’ pear flowers with the pollen of ‘Yali’ (JY), ‘Suli’ (JS) and ‘Huangli’ (JH). The results indicated that the mass, transverse diameter and longitudinal diameter of the JY group were significantly higher than the JS group and JH group. On the other hand, the pears of the JY group and JS group obtained higher soluble sugar content. The aroma content of characteristic compounds was higher in the JY group than in the JS group and JH group. Multivariate analysis revealed significant differences in the nonvolatile metabolites among the JY group, JS group and JH group, potentially explaining the variations in the nutritional and flavor compounds of the pears. Furthermore, this research investigated metabolic changes in the pears during development and ripening under the three types of pollination. The results showed that amino acid metabolism differed among these pollination types during development. These differences may be the cause of the observed variations in the pears. This research clarified the effect of xenia on the nutritional components and flavor substances in the ‘Jingbaili’ pear and could provide data support for improving the quality of the ‘Jingbaili’ pear.
研究领域:
园艺学
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植物生理学
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食品科学
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DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2015
·Vol. 30
·期 1
·75-80
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IF 1.723
Congcong Wen,Zhiyi Wang,Meiling Zhang,Shuanghu Wang,Peiwu Geng,Fa Sun,Mengchun Chen,Guanyang Lin,Lufeng Hu,Jianshe Ma,Xianqin Wang
Paraquat is quick-acting and non-selective, killing green plant tissue on contact; it is also toxic to human beings and animals. In this study, we developed a urine metabonomic method by gas chromatography–mass spectrometry to evaluate the effect of acute paraquat poisoning on rats. Pattern recognition analysis, including both partial least squares discriminate analysis and principal component analysis revealed that acute paraquat poisoning induced metabolic perturbations. Compared with the control group, the levels of benzeneacetic acid and hexadecanoic acid of the acute paraquat poisoning group (intragastric administration 36 mg/kg) increased, while the levels of butanedioic acid, pentanedioic acid, altronic acid decreased. Based on these urinary metabolomics data, support vector machine was applied to discriminate the metabolomic change of paraquat groups from the control group, which achieved 100% classification accuracy. In conclusion, metabonomic method combined with support vector machine can be used as a useful diagnostic tool in paraquat-poisoned rats. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
毒理学
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分析化学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
Journal of Biophotonics
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2017
·Vol. 11
·期 5
·e201700245
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IF 4.328
Yueyue Jing,Yulan Wang,Xinyi Wang,Chuan Song,Jiong Ma,Yonghui Xie,Yiyan Fei,Qinghua Zhang,Lan Mi
The label-free imaging and spectroscopy method was studied on cervical unstained tissue sections obtained from 36 patients. The native fluorescence spectra of tissues are analyzed by the optical redox ratio (ORR), which is defined as fluorescence intensity ratio between NADH and FAD, and indicates the metabolism change with the cancer development. The ORRs of normal tissues are consistently higher than those of precancer or cancerous tissues. A criterion line of ORR at 5.0 can be used to discriminate cervical precancer/cancer from normal tissues. The sensitivity and specificity of the native fluorescence spectroscopy method for cervical cancer diagnosis are determined as 100% and 91%. Moreover, the native fluorescence spectroscopy study is much more sensitive on the healthy region of cervical precancer/cancer patients compared with the traditional clinical staining method. The results suggest label-free imaging and spectroscopy is a fast, highly sensitive and specific method on the detection of cervical cancer.
研究领域:
医学
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生物光学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Weiwang Zheng,Jing Wu,Nannan Song,Baonan Zhang,Chunhui Jin,Weibo Zhao,Kai Wang,Shenghua Wang,Xiaodan Zhu,Cui Sun
Detection of γ-glutamyltransferase (GGT) activity in cancer cells is crucial for understanding tumor progression and metabolic dysregulation. However, the accurate detection of GGT remains challenging due to its complex intracellular distribution and interactions with other enzymes. This study introduces a novel hemicyanine-based sensor, named GP, designed for the sensitive and real-time detection of GGT in tumor cells. By leveraging the unique optical properties of hemicyanines in the near-infrared spectrum, the GP sensor enables deeper tissue penetration and minimizes background interference, which is essential for tumor imaging. The sensor incorporates a GGT-specific recognition sequence that selectively binds to GGT, facilitating precise monitoring of its enzymatic activity through fluorescence signals generated by intramolecular charge transfer (ICT). Our results show that the GP probe exhibits high selectivity for GGT, distinguishing it from other biological molecules. Evaluation under tumor-relevant conditions confirms the probe’s robustness for both research and clinical applications. In cancer cell models, GP successfully detects increased GGT activity, suggesting its potential for non-invasive monitoring of tumor dynamics. Overall, this study demonstrates GP as a promising tool for the accurate detection of GGT activity, with significant implications for understanding cancer-related metabolic changes and tumor biology.
研究领域:
生物化学
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肿瘤学
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医学影像
✓ 已验证
DOI
PubMed
高影响因子
Environmental Science-Nano
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2025
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IF 5.1
Ying Wang,Fuming Wang,Xuyang Feng,Haoyou Jiang,Hualin Zhang,Yongfang Qian,Botian Zhu,Yaping Huang,Yimin Zhu
In recent years, microbial pollution has become a serious environmental problem, and the release of microorganisms into the water environment seriously threatens human health. As environment-friendly and low-cost antibacterial agents, Mg(OH)2 nanoparticles (M-NPs) have garnered considerable attention for their small size, innocuity, no drug resistance, chemical stability and thermal stability. However, little is known about the physiological changes that bacteria undergo in the presence of M-NPs. In this work, the antibacterial mechanism of M-NPs synthesized by applying the coprecipitation method was investigated using Escherichia coli (E. coli) as a model system. The oxygen vacancies on the M-NP surface, which can produce reactive oxygen species (ROS, ·O2−, H2O2, and ·OH), were examined via O2-temperature programmed desorption (O2-TPD). Abnormality in three central metabolic pathways (energy, glucose and tricarboxylic acid cycle) induced by M-NPs was detected by analyzing the activity of respiratory chain dehydrogenase, gluconokinase (GK) and succinate dehydrogenase (SDH). The downregulated activity and gene expression levels of GK confirmed that M-NPs play an inhibitory role, and these physiological changes result in cell death. Thus, M-NPs have great potential in the field of preventing and controlling microbial pollution.
研究领域:
微生物学
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环境科学
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产品关联
DOI
高影响因子
Biomedical Optics Express
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2020
·Vol. 11
·期 4
·1977-1990
·
IF 3.921
Xinyi Wang,Xinyi Wang,Yulan Wang,Yulan Wang,Zixiao Zhang,Maojia Huang,Yiyan Fei,Jiong Ma,Jiong Ma,Jiong Ma,Jiong Ma,Lan Mi,Lan Mi
This study proposed label-free fluorescence lifetime imaging and phasor analysis methods to discriminate different grades of cervical intraepithelial neoplasia (CIN). The human cervical tissue lesions associated with cellular metabolic abnormalities were detected by the status changes of important coenzymes in cells and tissues, reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Fluorescence lifetime imaging microscopy (FLIM) was used to study human cervical tissues, human cervical epithelial cells, and standard samples. Phasor analysis was applied to reveal the interrelation between the metabolic changes and cancer development, which can distinguish among different stages of cervical lesions from low risk to high risk. This approach also possessed high sensitivity, especially for healthy sites of CIN3 tissues, and indicated the dominance of the glycolytic pathway over oxidative phosphorylation in high-grade cervical lesions. This highly adaptive, sensitive, and rapid diagnostic tool exhibits a great potential for cervical precancer diagnosis.
研究领域:
医学
·
细胞生物学
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DOI
PubMed
高影响因子
PHARMACOLOGY
·
2017
·
IF 1.442
Zhang Jing,Song Huanchun,Jiang Shuying,Chen Zhibin,Tong Shuhua,Lin Feiyan,Wen Congcong,Zhang Xiuhua,Hu Lufeng
Background: Aspirin and ibuprofen are the most frequently prescribed non-steroidal anti-inflammatory drugs in the world. However, both are associated with a variety of toxicities. We applied serum metabonomics and Fisher discrimination for the early diagnosis of its toxic reaction in order to help diagnose these toxicities. Methods: A total of 45 rats were randomly divided into Control group, Aspirin group, and Ibuprofen groups. The experiment groups were given intragastric aspirin (15 mg/kg) or ibuprofen (15 mg/kg) for 3 weeks. Liver function tests were performed and blood metabonomics were analyzed by gas chromatography-mass spectrometry. Results: The most important compounds altered were trihydroxybutyric acid and l-alanine in the aspirin group, and acetoacetic acid, l-alanine, and trihydroxybutyric acid in the ibuprofen group. With respect to metabolic profiles, all 3 groups were completely distinct from one another. Fisher discrimination showed that 91.1% of the original grouped cases were correctly classified by the third week. However, only 55.6% of liver function tests were able to classify grouped cases correctly. Conclusion: Trihydroxybutyric acid, l-alanine, and acetoacetic acid were the most significant indicators of altered serum metabolites following intragastric administration of aspirin and ibuprofen in rates. These metabolomic data may be used for classification of aspirin and ibuprofen toxicity
研究领域:
药理学
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毒理学
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代谢组学
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生物统计学
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产品关联
DOI
PubMed
高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2021
·
IF 2.674
Liming Chang,Bin Wang,Meihua Zhang,Jiongyu Liu,Tian Zhao,Wei Zhu,Jianping Jiang
Corticosterone (CORT)-mediated adaptive plasticity improves animal fitness in stressful environments. Although it brings ecological benefits, the cost potentially constrains its expression and evolution. Revealing the factors affecting plasticity costs is of great ecological and evolutionary significance. Evidence indicates that both CORT and background colour can induce metabolic changes in animals, which in turn determine phenotypic plasticity . However, whether and/or how CORT and background colour jointly act on plastic responses has not been studied. Here, this question has been investigated in amphibian tadpoles ( Microhyla fissipes ) exposed to CORT at different background colours (white or black) using integrated morphological, histological, and transcriptomic analyses. The results showed that CORT exposure increased relative tail length, immune function, and metabolic maintenance (i.e., transcription of substrate catabolism and oxidative phosphorylation) at the expense of reduction in growth rate and skin melanin level. The black background also increased relative tail length and metabolic maintenance (i.e., transcription of oxidative phosphorylation) at the cost of reduction in growth rate, but increased skin melanin level. The expression of critical pigmentation genes indicated that black background activated a distinct and opposite pigmentation regulating route to CORT. Although there was no interactive effect of background colour and CORT on phenotypic and metabolic variations, their additive effects further impact the trade-off between somatic growth, metabolic maintenance, and pigmentation in terms of resource allocation. In conclusion, the individual and additive effects of background colour and CORT exposure on tadpole plasticity were revealed. These results likely provide new insights into the environmental adaptation of animals.
研究领域:
生态学
·
进化生物学
·
生理学
✓ 已验证
DOI
PubMed
高影响因子
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