Purpose To analyse phytochemical composition, antioxidant potential, antimicrobial activity and α-amylase, α-glucosidase and xanthine oxidase inhibitory potential of corn silk extracts derived from the Syngenta 5414 corn variety. Methods Corn silk sample was lyophilized and extracted using 80% ethanol, methanol, water or acetone. Phytochemicals were qualitatively screened and bioactive compounds were identified through GCMS. Antimicrobial activities were evaluated using the agar well method for bacteria and fungal biomass method for fungi. The extracts were subjected to evaluation for their antidiabetic activity through α-amylase and α-glucosidase inhibitory assays, while their anti-gout potential was assessed through xanthine oxidase inhibitory assay, encompassing all four solvent extracts in the study. Results Phytochemical screening revealed the presence of phenols, flavonoids, tannins, alkaloids, sterols, protein xanthoproteic, and cardiac glycosides in the extracts. The Folin-Ciocalteu and AlCl 3 assays demonstrated higher yields of phenolics and flavonoids in 80% ethanolic extract. Furthermore, the extracts remarkably scavenged reactive oxygen species like DPPH, ABTS and elicited considerable ferric ion reducing antioxidant power. GC–MS analysis identified 21 compounds in the 80% ethanolic extract, with 1,2,3-Propanetriol-1-acetate, n-Hexadecanoic acid, and 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- as major components. Antimicrobial assays demonstrated the ethanolic (80%) and methanolic extract's remarkable ability to inhibit the growth of Gram-positive bacteria and exhibit anti-fungal properties. The ethanolic (80%) extract exhibited an IC 50 value of 0.70 ± 0.02 and 0.71 ± 0.01 mg against α-amylase and α-glucosidase, respectively, and 33.76 ± 0.43 mg against xanthine oxidase. Conclusion Corn silk exhibits potential as a valuable reservoir of natural bioactive compounds endowed with a wide array of health-promoting properties. Graphical
Hongjun Song,Weijie Zhang,Ya Zhang,Caixia Yin,Fangjun Huo
Intracellular viscosity plays an important role for maintaining the diffusion, transportation, signal transmission and cell metabolism between biomolecules. And abnormal intracellular viscosity would cause various concurrent diseases such as fatty liver, hyperlipidemia and so on. However, as far as we know, there are quite few tools could be used for imaing of intracellular viscosity, much less in-depth investigation of the viscosity-related dysfunctional and disease. Herein, we have designed and developed a series of NIR fluorescent probes , in which the chemical bond between fluorophore and molecular rotor was designed with single bonds, double bonds, and extended double bonds, respectively. According to the theoretical calculations and experiments results, BP-III with was selected as the most sensitive fluorescence sensor for imaging viscosity change in vitro and in vivo. As the viscosity increasesd, the TICT (twisted intramolecular charge transfer) of BP- III was inhibited, accompanied with a strong red fluorescence enhancement at 740 nm. In addition, BP- III showed excellent mitochondrial target ability and can be used to imaging viscosity changes in mitochondrial of living cells. More importantly, the probe has been used for imaging the abnormal viscosity of fatty liver at the organ level. We expect this work would provide meaningful tool for promoting fundamental research in biological viscous environment and mitochondrial viscosity related diseases.
危险品化学品经营许可证（带存储）