SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Jiahui Fan,Yangyang Jia,Dongdong Xu,Zhe Ye,Jiahang Zhou,Jionghao Huang,Yulong Fu,Chaofeng Shen
Significant microbial removal of highly chlorinated polychlorinated biphenyls (PCBs) requires the cooperation of anaerobic and aerobic bacteria. During the sequencing process of anaerobic dechlorination and aerobic degradation of PCBs, aerobic degrading bacteria have to undergo anaerobic stress. However, the survival strategy of aerobic degrading bacteria under anaerobic condition is not well-understood. In this study, the culturable cells of Rhodococcus biphenylivorans TG9 decreased from 10 8 CFU/mL to values below the detection limit after 60 days of anaerobic stress while the viable cells remained 10 5 –10 6 cells/mL, indicating that anaerobic condition induced TG9 entering into the viable but nonculturable (VBNC) state. Cell resuscitation was observed when oxygen was supplied further confirming the VBNC state of TG9. The results of single-cell Raman spectroscopy combined with heavy water indicated the significant decrease of metabolic activity after TG9 entering into the VBNC state. Additionally, the degradation ability of TG9 in the VBNC state was also significantly reduced, while it recovered after resuscitation. Our research proved that entering into the VBNC state is a survival strategy of TG9 under anaerobic conditions, and the limited culturability and degrading capacity could be overcome by resuscitation. The present study provides new insights for improving the remediation efficiency of PCBs contamination.
研究领域:
微生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2022
·Vol. 109
·期 6
·1043-1050
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IF 2.807
Qv Wenrui,Wang Xilin,Li Ningyun,Du Jingjing,Pu Gaozhong,Zhang Hongzhong
In this study, exposure experiments were conducted to assess the effects of polystyrene nanoparticles (PS) and amine-modified polystyrene nanoparticles (APS) at environmental concentrations (1, 10, and 100 µg L − 1 ) on two fungal species ( Geotrichum candidum and Aspergillus niger ), isolated from leaf litter in streams, concerning their growth and metabolic activity. Results showed that PS at 1 and 10 µg L − 1 have hormesis effects on G. candidum growth. Compared with G. candidum , A. niger had higher sensitivity to nanoplastic exposure. Besides, the peroxidase and cellobiohydrolase activities of A. niger were significantly inhibited by nanoplastics (except 1 µg L − 1 PS), which would weaken its metabolic activity in carbon cycling. These results provided a new thought on how the growth and functions of aquatic fungi cope with the stress induced by nanoplastics. Overall, the study provided evidence for the different responses of aquatic fungi to nanoplastics in streams.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PEST SCIENCE
·
2025
·
IF 4.1
Lin Kai,Wu Hongxin,Li Zhongsheng,Zhong Zichun,He Liuyan,Guo Yujing,Zhang Jie,Xu Xiaoxia,Zhang Wenqing,Jin Fengliang,Pang Rui
Insecticide resistance in pest control poses a threat to agricultural production and human health. Numerous insect species express genes coding for detoxification enzymes that have broad substrate promiscuity thus conferring resistance to various insecticides. However, whether the homologs of these genes play similar roles in resistance phenotypes of closely related species remains largely unclear. Therefore, this study compares the resistance profiles of three major rice planthopper species (Delphacidae) ( Laodelphax striatellus , Nilaparvata lugens , and Sogatella furcifera ) based on the metabolic activity of their cytochrome P450s. Genome-wide analyses resulted in 68, 70, and 64 P450 genes in L. striatellus , N. lugens , and S. furcifera , respectively. Phylogenetic analyses among these genes found that most resistance-related genes in one species had homologs in other planthopper species. The most resistance-relevant orthogroup ( CYP6ER s) showed higher evolutionary instability than most other groups. RNAi and in vitro metabolism assays revealed that CYP6ER s confers more divergent insecticide resistance profiles among planthopper species than the other two major resistance-related P450 subfamilies ( CYP6AY s and CYP4C61 s). Alphafold-based structural predictions and alignments suggested that P450 orthogroups with higher phylogenetic instability tended to have less structural similarities, resulting in more divergent metabolic profiles. This relationship was also in silico validated on Aphidae aphids and Lepidoptera noctuids. This study proposes combined phylogenetic and toxicogenomic analyses for understanding CYPome-based insecticide resistance convergency and divergency among closely related pests. These findings may improve the accuracy and rationality of chemical pest control.
研究领域:
昆虫学
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毒理学
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基因组学
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产品关联
DOI
高影响因子
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
·
2024
·
IF 2.7
Guofang Bi,Fengting Liang,Ting Wu,Peng Wang,Xiaowen Jiang,Shuang Hu,Chenghua Wu,Wenhong Zhou,Jiayin Guo,Xiao Yang,Jian-hong Fang,Wenying Chen,Huichang Bi
Human pregnane X receptor (PXR) is critical for regulating the expression of key drug-metabolizing enzymes such as CYP3A and CYP2C. Our recent study revealed that treatment with rodent-specific PXR agonist pregnenolone-16α-carbonitrile (PCN) significantly induced hepatomegaly and promoted liver regeneration after two-thirds partial hepatectomy (PHx) in mice. However, it remains unclear whether PXR activation induces hepatomegaly and liver regeneration and simultaneously promotes metabolic function of the liver. Here, we investigated the metabolism activity of CYP1A2, CYP3A1/2 and CYP2C6/11 during PXR activation-induced liver enlargement and regeneration in rats after cocktail dosing of CYP probe drugs. For PCN-induced hepatomegaly, a notable increase in the metabolic activity of CYP3A1/2 and CYP2C6/11, as evidenced by the plasma exposure of probe substrates and the AUC ratios of the characteristic metabolites to its corresponding probe substrates. The metabolic activity of CYP1A2, CYP3A1/2 and CYP2C6/11 decreased significantly after PHx. However, PCN treatment obviously enhanced the metabolic activity of CYP2C6/11 and CYP3A1/2 in PHx rats. Furthermore, the protein expression levels of CYP3A1/2 and CYP2C6/11 in liver were up-regulated. Taken together, this study demonstrates that PXR activation not only induces hepatomegaly and liver regeneration in rats, but also promotes the protein expression and metabolic activity of the PXR downstream metabolizing enzymes such as CYP3A1/2 and CYP2C6/11 in the body.
研究领域:
药理学
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肝病学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Xue Liu,Zheng Zhang,Qingwen Zheng,Chengcheng Suo,Bailing Dong,Huiying Song,Jiayi Wang,Jia Liu,Runfeng Yuan,Sailike Milanbieke,Sha Luo,Chenhui Yang,Zhijun Chen,Ruiwen Wang,Wei Li,Shouxin Liu
Electroactive biofilms (EABs) are essential components of both natural and artificial bio-electrochemical systems (BESs). However, the inevitable decay of EABs during prolonged operation can diminish their performance. In this contribution, an effective and noninvasive strategy for rejuvenating aging biofilms by the elastic deformation of anode material is approved. The synthesized wood tracheid-like structures anode material showed excellent compressibility and fatigue resistance in a wet state. The findings indicate that after the elastic deformation of the anode, aged biofilm exhibited a 37.5% increase in metabolic activity, and multi-SIM images confirmed the removal of dead cells. Analysis of the extruded substance revealed a significant removal of loosely bound extracellular polymeric substance which doesn't contribute directly to electron transfer. Community analysis demonstrated the rejuvenation process suppressed the ecological competition from non-exoelectrogens. Overall, there is a notable 25.97% increase in power density following the elastic deformation of the anode. Additionally, ion diffusion, specific capacitance, and catalytic response current all improved. To the knowledge, this is the first report employing an anode deformation strategy to restore decayed mix-cultured electroactive biofilm, which is vital for the practical long-term application of BESs. This work also offers new insights into the mechanical influence of anode materials on microorganisms.
研究领域:
微生物学
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生物电化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2017
·
IF 6.942
Lingzhan Miao,Peifang Wang,Chao Wang,Jun Hou,Yu Yao,Jun Liu,Bowen Lv,Yangyang Yang,Guoxiang You,Yi Xu,Zhilin Liu,Songqi Liu
Environmental concerns regarding the potential ecological risks of metallic oxide nanoparticles (MNPs) in aquatic ecosystems are increasing; sediment is considered a sink for these MNPs. Although several studies have studied the potential impact of MNPs on microbial communities in freshwater and estuarine sediments, limited information is available regarding the influence of MNPs on the metabolic activity of surficial sediment microbial communities and related biogeochemical conditions. To address these issues, a microcosm approach was established to study the metabolic response of surficial sediment microbial communities to a single addition of TiO 2 or CeO 2 NPs (5 mg/L) using oxygen microelectrodes, enzyme activity measurements, and high-throughput sequencing. Rapid sedimentation of MNPs (regardless of NP type) was observed in freshwater samples, and most (up to 85%) accumulated in surface sediments (<5 mm). Microelectrode profile measurements in pre-incubated sediments treated with MNPs showed that the oxygen concentration decreased at a slower rate with increasing sediment depth compared to that in untreated controls. Biological oxygen consumption in the uppermost sediment layer (0–1500 μm) was significantly inhibited by MNPs, as calculated from steady-state microprofiles, with CeO 2 NPs resulting in enhanced acute toxicity than TiO 2 NPs. High-throughput sequencing showed that MNP exposure increased the bacterial diversity and altered the bacterial community structure, regardless of NP type. The abundance of three dominant bacterial genera, Methylotenera , Cytophagceae_uncultured (classified as an aerobic bacterium), and Cyanobacteria_norank (a facultative bacterium), was markedly reduced by MNPs, which was primarily responsible for inhibiting microbial-mediated oxygen consumption in surficial sediments. In summary, short-term exposure to MNPs negatively affected the metabolic activity of benthic microbial communities, which could influence the biogeochemical functions along the sediment-water interface.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Shuangshuang Wan,Wurui Liu,Qian Wu,Kepeng Wang,Yaocheng Li,Peihong Huang,Yu Wu,Yunfei Mu,Yulin Fan,Jun Tao,Jia Yao,Fei Peng,Yu Zou,Lianhui Wang,Zhiyao Yuan,Xianguang Ding
Extracellular vesicles (EVs) hosting enzymatic activities that function as independent metabolic units are attractive natural biocatalytic platforms. However, directly using these metabolically active nanoreactors for effective biocatalytic applications remains challenging, mainly due to their constrained catalytic capabilities. Here, we construct an EV-templated nanobiohybrid system by engineering an EV surface with a photoresponsive zeolitic imidazolate framework (ZIF). The deposition of ZIF nanostructures on EVs not only contributes to improved biocatalytic stability but also enables interfacial coupling between photoexcited electrons from the ZIF and the enzymatic reaction of metabolically active EVs. Nearly 300% of biomass conversion efficiency increment could be achieved by EVs derived from macrophages. This enhanced biocatalysis, high catalytic stability, and low cytotoxicity endowed the EV@ZIF nanosystem with robust biosynthesis and antimicrobial activity. When evaluated in a mouse periodontitis model, we show that the autologous biocatalytic EV@ZIF demonstrated efficient therapeutic capability by killing bacteria and inhibiting inflammation. This nanoengineering strategy will benefit the future optimization of metabolically active EV nanoreactors as biocatalysts for a broad range of therapeutics.
研究领域:
生物医学工程
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纳米医学
·
酶学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
·
2018
·Vol. 365
·期 2
·398-407
·
IF 3.706
Jie Gao,Zhao Wang,Gao-Ju Wang,Hong-Xin Zhang,Na Gao,Jie Wang,Cai-E. Wang,Zhao Chang,Yan Fang,Yun-Fei Zhang,Jun Zhou,Han Jin,Hai-Ling Qiao
Hepatocellular carcinoma (HCC) is one of the most common types of primary liver cancer and the third most frequent cause of cancer death worldwide. Diethylnitrosamine (DEN) is one of the recognized risk factors for hepatocarcinogenesis likely due to CYP2E1-mediated metabolic activation. However, CYP2E1-mediated DEN metabolic activity in non-neoplastic liver tissue from HCC patients has not been determined; the role of CYP2E1 activity, in particular CYP2E1 constitutive activity and CYP2E1 inhibited activity, with respect to the hepatocarcinogenesis induced by DEN is not yet clear. Herein, we determined CYP2E1-mediated DEN metabolic activity in non-neoplastic liver tissue from HCC patients and found that CYP2E1-mediated DEN metabolic activity was significantly elevated with a 43.3% positive rate, and clinicopathologic parameters did not affect the activity. Then, using a Sprague-Dawley rat liver tumor model induced by DEN, the relationship between CYP2E1 constitutive/inhibited activity and hepatocarcinogenesis was explored. The results showed that the CYP2E1 constitutive activity was strongly correlated with tumor incidence and severity of liver tumorigenesis (nodule numbers and size), whereas inhibition of CYP2E1 activity decreased hepatocyte proliferation, liver injury, and liver carcinogenesis in the presence of DEN. In conclusion, the higher CYP2E1 activity would lead to an increased incidence of HCC as a result of CYP2E1-mediated DEN activation. Therefore, higher CYP2E1 activity might be a risk factor for HCC induced by DEN.
研究领域:
肿瘤学
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药理学
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毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Shuyue Zheng,Jihong Dong,Quan Chen,Min Wu,Dong Zhu,Li Cui,Philippe Frangois-Xavier Corvini,Hong-Zhe Li,Bo Pan
Polycyclic aromatic hydrocarbons (PAHs) are widespread contaminants that can accumulate in microorganisms, posing significant ecological risks. While previous studies primarily focused on PAH concentrations, the impacts of PAH self-clustering have been largely overlooked, which will lead to inaccurate assessments of their ecological risks. This study evaluates the toxic effects of four prevalent PAH clusters on microbes with an emphasis on comparing the cluster sizes. Results revealed that over 95% of PAHs can form clusters in the aquatic environment, with smaller clusters more likely to form at lower concentrations and with fewer benzene rings. To quantify the toxic effects and understand underlying mechanisms, single-cell Raman-D2O was employed to link bacterial phenotypes with transcriptomic profiles. Bacteria exposed to smaller PAH clusters showed a 1%–10% reduction in metabolic activity, which was associated with a 1.8–2.9-fold increase in intracellular reactive oxygen species (ROS). Furthermore, when exposed to smaller PAH clusters, the expression of genes related to the ROS response and efflux pumps was upregulated by up to 6.33-fold and 4.97-fold, respectively, suggesting that smaller PAH clusters pose greater toxicity to microbes. These findings underscore the potentially overlooked risks of PAH clusters in environmental systems and deepen our understanding of the environmental fate and ecological risks of these contaminants.
研究领域:
环境科学
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微生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2019
·Vol. 7
·期 2
·665-675
·
IF 7.704
Jun Hou,Tengfei Li,Lingzhan Miao,Guoxiang You,Yi Xu,Songqi Liu
The widespread application of TiO2 NPs will lead to their increasing release into the aquatic environment and thus affects the ecosystem function of aquatic life. As a microbial community, responses of periphytic biofilm to toxicity of TiO2 NPs are largely unknown, in particular with respect to dynamic shifts of community structure and microbial functions of periphytic biofilms. Therefore, this study investigated the relationship between microbial functions and community composition of periphytic biofilms after chronic exposure (21 days) to 50 μg L−1 and 500 μg L−1 TiO2 NPs. The increase of DOC in surface water was inhibited during the first 11 days (49.55%) by 500 μg L−1 TiO2 NPs and then recovered. Distinct responses were observed between community structure and microbial functions of biofilms after exposure to TiO2 NPs. Specifically, both 50 μg L−1 and 500 μg L−1 TiO2 NPs promoted the growth of microalgae (78.85–134.86%) and altered the bacterial community composition at day 7, and these trends lasted at day 21. In terms of the microbial functions determined using Biolog™ ECO microplates, biofilms maintained their total carbon metabolic activities under the stress of 50 μg L−1 TiO2 NPs. However, the carbon metabolic activities were significantly inhibited (24.63%) by the exposure to 500 μg L−1 TiO2 NPs at day 7 and then recovered to the control level at day 21. Although inhibition still existed in the metabolic activity for carbohydrate (17.00%) after long-term exposure, periphytic biofilms exhibited resistance to TiO2 NPs and continuous decrease in nanotoxicity was observed.
研究领域:
环境微生物学
·
水生生态学
·
纳米毒理学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Qian Feng,Laiwei Luo,Xindi Chen,Kaijie Zhang,Fang Fang,Zhaoxia Xue,Chao Li,Jiashun Cao,Jingyang Luo
The N-Acy-L-homoserine lactones (AHLs) mediated quorum sensing (QS) system exhibited important ecological significance in bacterial biofilm formation. However, the previous studies mainly focused on indigenous AHLs while the role of exogenous AHLs has remained unclear. This study evaluated the roles of exogenous AHLs on the biofilm formation of Pseudomonas aeruginosa . Both the C6-HSL and C8-HSL promoted the biofilm formation of P. aeruginosa with an enhancement of 2.47 and 1.88 times, respectively. Further analysis showed that exogenous AHLs contributed greatly to the adhesive ability instead of growth rate. Also, the bacterial motility and metabolic activities were significantly improved by AHLs. Moreover, the microbial functional genes ( i.e. lasI , lasR , rhlI and rhlR ) involved in regulating the biofilm formation were highly expressed in AHLs reactors. These findings expanded the knowledge of AHLs functions in mediating biofilm formation, and provided insightful guidance on the biofilm regulation in the wastewater treatment via biofilm technology.
研究领域:
微生物学
·
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Huilin Chen,Maomao Xie,Wenjie Li,Lipin Tan,Xiaoming Cai,Mingjing Shen,Ruibin Li
The extensive use of synthetic antioxidants, notably N-(1,3-dimethylbutyl)-N′-phenyl- p -phenylenediamine (6-PPD), in rubber-related products, particularly in tire manufacturing, has induced concerns regarding their environmental impact and potential health hazards. Despite the identification of 6-PPD and its derivative, 6-PPD quinone (6-PPDQ), in various water samples and their lethal effects on certain aquatic species ( e.g. , coho salmon, rainbow trout and brook trout), the levels of airborne 6-PPD/6-PPDQ and their respiratory toxicity remain relatively unexplored. In this study, we aimed to evaluate the respiratory toxicity potential of 6-PPD and its derivatives, with a specific focus on detecting these compounds in airborne particulates and assessing their toxic effects on lung cells. Characterization of four airborne fine particulate (FP) samples revealed spherical morphologies with diameters ranging from 17.7 to 225.7 nm, displaying slight agglomeration and negative surface charge. methanol/acetonitrile extraction followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis confirmed the presence of both 6-PPD and 6-PPDQ on the surfaces of FPs, with significant variations (0.26–1.05 μg g −1 ) in loading capacity observed among the samples. Subsequent exposure of lung cells (THP-1, BEAS-2B, and A549) to 6-PPD and 6-PPDQ revealed dose-dependent declines in mitochondrial metabolic activity induced by 6-PPD, along with severe membrane damage, ATP depletion, and pro-inflammatory cytokine release. Conversely, 6-PPDQ exhibited negligible toxicity in all tested parameters. These findings underscore the potential health risks associated with airborne 6-PPD exposure and emphasize the importance of further research into the respiratory toxicity of 6-PPD derivatives.
研究领域:
环境科学
·
毒理学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）