SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Pei Fan,Haihao Yu,Tian Lv,Huiyuan Wang,Dexiang Li,Chao Tong,Zhonghua Wu,Dan Yu,Chunhua Liu
Antibiotic pollution and biological invasion pose significant risks to freshwater biodiversity and ecosystem health. However, few studies have compared the ecological adaptability and ciprofloxacin (CIPR) degradation potential between alien and native macrophytes. We examined growth, physiological response , and CIPR accumulation, translocation and metabolic abilities of two alien plants ( Eichhornia crassipes and Myriophyllum aquaticum ) and one native submerged species ( Vallisneria natans ) exposed to CIPR at 0, 1 and 10 mg/L. We found that E. crassipes and M. aquaticum 's growth were unaffected by CIPR while V. natans was significantly hindered under the 10 mg/L treatment. CIPR significantly decreased the maximal quantum yield of PSII, actual quantum yield of PSII and relative electron transfer rate in E. crassipes and V. natans but didn't impact these photosynthetic characteristics in M. aquaticum . All the plants can accumulate, translocate and metabolize CIPR. M. aquaticum and E. crassipes in the 10 mg/L treatment group showed greater CIPR accumulation potential than V. natans indicated by higher CIPR contents in their roots. The oxidative cleavage of the piperazine ring acts as a key pathway for these aquatic plants to metabolize CIPR and the metabolites mainly distributed in plant roots. M. aquaticum and E. crassipes showed a higher production of CIPR metabolites compared to V. natans , with M. aquaticum exhibiting the strongest CIPR metabolic ability, as indicated by the most extensive structural breakdown of CIPR and the largest number of potential metabolic pathways. Taken together, alien species outperformed the native species in ecological adaptability, CIPR accumulation and metabolic capacity. These findings may shed light on the successful invasion mechanisms of alien aquatic species under antibiotic pressure and highlight the potential ecological impacts of alien species, particularly M. aquaticum . Additionally, the interaction of antibiotic contamination and invasion might further challenge the native submerged macrophytes and pose greater risks to freshwater ecosystems .
研究领域:
生态学
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环境科学
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水生生物学
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DOI
PubMed
高影响因子
Applied Sciences-Basel
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2021
·Vol. 11
·期 24
·11597
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IF 2.679
Jianying Zhao,Jing Qian,Ji Luo,Mingming Huang,Wenjing Yan,Jianhao Zhang
Featured ApplicationDuring food processing, the surface of food and processing equipment is easily contaminated by microorganisms, which will cause food spoilage or the spread of disease, threatening food safety. Plasma-activated solution can be used as a novel food disinfectant, enhancing food quality. Plasma-activated solution will not leave chemical residues or cause environmental pollution. It is a green and environmentally friendly disinfectant and promises to replace traditional chemical disinfectants.AbstractPlasma-activated solution has attracted more attention in the food industry due to no chemical residue and good bacteriostatic properties. This study aimed to evaluate the effects of plasma-activated hydrogen peroxide solution (PAH) on the morphophysiology ofStaphylococcus aureusbiofilms. PAH was prepared using dielectric-barrier-discharge plasma and incubated withS. aureusbiofilms for 0–40 min. Changes in biofilm morphophysiology were evaluated with laser scanning confocal microscopy, electron microscopic images, reactive oxygen species (ROS) content, metabolic capacity, and 1% agarose gel. Results indicated that the population ofS. aureusin the biofilms was reduced by 4.04-log after incubation with PAH for 30 min. The thickness and metabolic capacity of biofilms were decreased, the ROS content and DNA fragments of bacteria increased after PAH treatments. Data suggested that PAH treatments significantly destroyed the morphophysiology ofS. aureus(ATCC 6538) biofilms and could be considered as a valuable anti-biofilm technology to reduce foodborne pathogens on food and/or in food facilities.Keywords:foodborne pathogen;cold plasma;nonthermal antimicrobial treatment;metabolic capacity;DNA damage
研究领域:
食品科学
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微生物学
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DOI
高影响因子
Pharmacogenomics & Personalized Medicine
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2021
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IF 3.912
Magesa Mafuru,Sanlan Wu,Henry Mayala,Zaituni Msengwa,Amani Phillip,Charles Mgone
Purpose To analyze the combined effect of CYP2C19 genetic polymorphism and PPIs coadministration on voriconazole trough concentration (VCZ-Ctrough) in Chinese patients with hematological disorders.Patients and Methods A prospective observational study involved 250 plasma samples from 114 adult patients receiving voriconazole with or without PPIs were analyzed. Demographics and clinical characteristics were obtained from patient’s records. A validated LC-MS/MS was used to quantify the plasma VCZ-Ctrough. Genotyping for CYP2C19*2 and CYP2C19*3 variant alleles was performed by PCR-RFLP followed by DNA sequencing. The combined total score (from 2 to 5) was calculated for each patient. The higher the score, the lesser the metabolism of the patient.Findings Fifty percent of patients administered with voriconazole were coadministered with PPIs, predominantly omeprazole or esomeprazole. Patients exhibiting CYP2C19 poor metabolizer phenotype showed a significantly higher median VCZ-Ctrough, (4.31µg/mL [IQR, 1.64µg/mL–7.36µg/mL]) than patients with normal metabolizer (1.38µg/mL, [IQR, 0.79µg/mL–2.14µg/mL], p < 0.0001). Similarly, patients co-administration with PPIs had higher median VCZ-Ctrough (2.86µg/mL [IQR 1.33µg/mL–4.66µg/mL]), than PPIs non-users (1.71µg/mL, [IQR, 0.86µg/mL–3.48µg/mL], p = 0.001). However, we noted that the median VCZ-Ctrough for each factor was ranging within the normal recommended therapeutic range in the Chinese population (0.5µg/mL–5µg/mL). But when the two factors were combined, the median VCZ-Ctrough was steadily increasing as the metabolic capacity (reflected by combined total score) was increasing. Importantly, the median VCZ-Ctrough in PM/PPIs user (total score 5) was significantly elevated to supra-therapeutic levels compared to NM/PPI non-user group (total score 2) (5.83µg/mL [IQR, 2.19µg/mL–9.51µg/mL] versus 1.13µg/mL [IQR, 0.67µg/mL–1.82µg/mL]), respectively, P < 0.0001. Furthermore, we observed that the elevation of median VCZ-Ctrough to supra-therapeutic levels was largely contributed by omeprazole or esomeprazole compared to lansoprazole or pantoprazole.Conclusion Coadministration with PPIs significantly increased voriconazole trough concentrations and there was an additive effect in CYP2C19 PMs, who were most likely to have supra-therapeutic levels.
研究领域:
药理学
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临床医学
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遗传学
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DOI
PubMed
高影响因子
Animals
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2025
·Vol. 15
·期 16
·2370
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IF 2.7
Can Zhang,Bo Cheng,Lei Xue,Ling Liu,Fenglai Wang,Jianjun Zang
Corn distiller’s dried grains with solubles (DDGSs) are a highly attractive alternative to corn and soybean meal in animal feeds. Accurately determining the net energy (NE) value of corn DDGSs is essential for formulating precise diets for pregnant sows. The substitution method has been widely employed to determine the available energy value of feed ingredients that cannot be fed directly to pigs. However, the appropriate substitution level of corn DDGSs in the test diets of the substitution method, which influences the accuracy of the assessed energy, and its corresponding net energy in pregnant sows remain unclear. Therefore, this study aims to establish the appropriate substitution level of corn DDGSs in test diets relative to the energy-supplying portion of the basal diet, and to accurately measure the digestive energy, metabolizable energy and NE values of different corn DDGSs types in pregnant sows. Based on an established appropriate substitution level for corn distillers dried grains with solubles (DDGSs) replacing energy-supplying components in the basal diet for pregnant sows, the substitution method was employed to determine the available energy values of corn DDGSs. In Exp. 1, forty pregnant sows (gestation day = 50 ± 5 d; body weight = 220 ± 24.9 kg; parity, 4 to 6) were randomly allocated into five treatments. The control group was fed a corn–soybean basal diet, while test diets contained 20%, 30%, 40%, 50% corn DDGSs replacing the energy-supplying portion of the basal diet. In Exp. 2, the available energy of five corn DDGS sources was determined using the substitution method at a 30% substitution level. Twelve pregnant sows (gestation day = 50 ± 5 d; body weight = 225.4 ± 29.2 kg; parity, 4 to 6) were arranged in a 6 × 3 Youden square design. Dietary treatments consisted of the corn–soybean basic diet and five test diets incorporating different corn DDGS types. Increasing the substitution level of corn DDGSs displayed both linear and quadratic effects ( p < 0.05) on the apparent total tract digestibility (ATTD) of dry matter (DM), organic matter (OM), acid detergent fiber (ADF), ether extract (EE) and gross energy (GE) in diets. The ATTD of neutral detergent fiber (NDF), digestible energy (DE) and metabolizable energy (ME) was quadratically affected by different substitution levels ( p < 0.05), with the highest value achieved at the 30% substitution level. As the substitution level of corn DDGSs increased, the ATTD of ADF in corn DDGSs decreased. In contrast, the ATTD of crude protein (CP) increased either linearly or quadratically ( p < 0.05), and a significant quadratic effect was observed for the ATTD of EE in corn DDGSs ( p < 0.05). Although the GE, DE, and ME of corn DDGSs were not significantly influenced by the substitution level, the 30% corn DDGSs resulted in the lowest coefficients of variation (CV). Therefore, a 30% substitution level of corn DDGSs is recommended for energy-supplying components in the basal diet of pregnant sows using the substitution method. The ranges of DE, ME and net energy (NE) of five corn DDGSs samples were 15.58–18.07, 12.17–16.42 and 8.76–15.88 MJ/kg DM, respectively. The absolute value of relative error (|RE|) between the predicted available energy values obtained from the prediction equations established in Exp. 2 and the determined values were below 5%, except for ME for corn DDGSs N3 (5.81%).
研究领域:
动物营养学
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畜牧学
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饲料科学
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能量代谢
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妊娠生理
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2017
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IF 6.198
Jun Tang,Ningyuan Zhu,Yan Zhu,Junzhuo Liu,Chenxi Wu,Philip Kerr,Yonghong Wu,Paul K. S. Lam
The toxic effects of nanoparticles on individual organisms have been widely investigated, while few studies have investigated the effects of nanoparticles on ubiquitous multicommunity microbial aggregates. Here, periphyton as a model of microbial aggregates, was employed to investigate the responses of microbial aggregates exposed continuously to Fe2O3 nanoparticles (5.0 mg L–1) for 30 days. The exposure to Fe2O3 nanoparticles results in the chlorophyll (a, b, and c) contents of periphyton increasing and the total antioxidant capacity decreasing. The composition of the periphyton markedly changes in the presence of Fe2O3 nanoparticles and the species diversity significantly increases. The changes in the periphyton composition and diversity were due to allelochemicals, such as 3-methylpentane, released by members of the periphyton which inhibit their competitors. The functions of the periphyton represented by metabolic capability and contaminant (organic matter, nitrogen, phosphorus and copper) removal were able to acclimate to the Fe2O3 nanoparticles exposure via self-regulation of morphology, species composition and diversity. These findings highlight the importance of both physiological and ecological factors in evaluating the long-term responses of microbial aggregates exposed to nanoparticles.
研究领域:
环境科学
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生态学
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微生物学
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DOI
PubMed
高影响因子
ADVANCED MATERIALS
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2024
·
IF 27.4
Zhuo Li,Boguang Yang,Zhengmeng Yang,Xian Xie,Zhengnan Guo,Jianyang Zhao,Ruinan Wang,Hao Fu,Pengchao Zhao,Xin Zhao,Guosong Chen,Gang Li,Fuxin Wei,Liming Bian
Cellular energetics plays an important role in tissue regeneration, and the enhanced metabolic activity of delivered stem cells can accelerate tissue repair and regeneration. However, conventional hydrogels with limited network cell adaptability restrict cell–cell interactions and cell metabolic activities. In this work, it is shown that a cell-adaptable hydrogel with high network dynamics enhances the glucose uptake and fatty acid β -oxidation of encapsulated human mesenchymal stem cells (hMSCs) compared with a hydrogel with low network dynamics. It is further shown that the hMSCs encapsulated in the high dynamic hydrogels exhibit increased tricarboxylic acid (TCA) cycle activity, oxidative phosphorylation (OXPHOS), and adenosine triphosphate (ATP) biosynthesis via an E-cadherin- and AMP-activated protein kinase (AMPK)-dependent mechanism. The in vivo evaluation further showed that the delivery of MSCs by the dynamic hydrogel enhanced in situ bone regeneration in an animal model. It is believed that the findings provide critical insights into the impact of stem cell–biomaterial interactions on cellular metabolic energetics and the underlying mechanisms.
研究领域:
生物材料
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干细胞生物学
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组织工程
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代谢工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）