Journal of Environmental Sciences
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2025
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IF 6.3
Qingmuke Su,Yaguang Du,Dongyun Du,Tian C. Zhang
Ciprofloxacin (CIP), as a quinolone antibiotic, has broad-spectrum antibacterial properties and can affect methanogenic performance in anaerobic digestion (AD). While previous studies focused on synthetic wastewater, the fate of CIP in real distillery wastewater (RDW) and its impact on microbial adaptation mechanisms remain unclear (such as biotransformation pathways, population dynamics, and the enzymes involved) in RDW is largely unclear. In this study, we investigated AD performance, metabolic pathways, and antibiotic resistance gene (ARG) dynamics using real wastewater spiked with CIP (0.3–2 mg/L). Results indicate that 0.5 mg/L CIP (631.83 mL CH 4 /g·VS) enhanced the methane yield by 6.67 % (592.34 mL CH 4 /g·VS in control), correlating with upregulated enzyme in glycosis, TCA cycle, and methanogenesis (F 420 increased). With full use of short-chain acids, transient volatile fatty acid (VFA) inhibition (≤ 50 mg/L on Day 3) was overcome by Day 10. Metagenomics revealed CIP promoted the production of stress proteins (e.g., cysteine synthase activity doubled). Furthermore, CIP (0.3–1 mg/L) suppressed mobile genetic elements (MGEs) encoding horizontal gene transfer, including isfinder (15.15 %) and integrases (6.25 %), while ARG and virulence factor abundances remained unchanged versus control. This study firstly shows that low-dose CIP in RDW increases methanogenesis via metabolic adaptation without exacerbating ARG risks. MGE suppression implies that CIP may lessen the possibility of ARG diffusion in AD systems. These results offer vital information for improving AD performance in the treatment of wastewater contaminated by antibiotics and developing methods to strike a balance between antibiotic removal and ARG control.
研究领域:
环境科学
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微生物学
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废水处理
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DOI
PubMed
高影响因子
RESEARCH IN MICROBIOLOGY
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2025
·
IF 3.4
Ping Lu,Juan Xue,Xuemeng Ji
Klebsiella pneumoniae is a major cause of endogenous endophthalmitis, a rapidly progressing intraocular infection associated with severe inflammation and vision loss. The vitreous body presents a hypoxic and iron-restricted environment, yet the bacterial metabolic adaptations that enable persistence in this niche remain largely unknown. Here, we show that K. pneumoniae undergoes metabolic reprogramming to facilitate intraocular survival, characterized by enhanced glycolysis and siderophore-mediated iron acquisition. Proteomic profiling under vitreous-mimicking conditions revealed significant upregulation of PfkA, PykF, and EntB. Targeted deletion of these genes impaired bacterial growth under hypoxia and iron limitation, and significantly reduced intraocular colonization, proinflammatory cytokine production, and visual impairment in a murine model. Double mutants lacking both glycolytic and iron acquisition pathways were nearly avirulent. Correspondingly, infected eyes exhibited lower levels of lactate and iron, reflecting reduced bacterial metabolic activity. These findings establish glycolysis and iron acquisition as critical determinants of K. pneumoniae virulence in the eye and provide insight into the metabolic strategies underpinning bacterial persistence in nutrient-limited host environments.
研究领域:
微生物学
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免疫学
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眼科学
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代谢
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发病机制
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传染病学
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DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Qian Cheng,Yuzhe Chen,Danyi Zou,Qilin Li,Xiaolei Shi,Qushuhua Qin,Miaodeng Liu,Lin Wang,Zheng Wang
The anti-tumor efficacy of current pharmacotherapy is severely hampered due to the adaptive evolution of tumors, urgently needing effective therapeutic strategies capable of breaking such adaptability. Metabolic reprogramming, as an adaptive survival mechanism, is closely related to therapy resistance of tumors. Colorectal cancer (CRC) cells exhibit a high energy dependency that is sustained by an adaptive metabolic conversion between glucose and glutamine, helping tumor cells to withstand nutrient-deficient microenvironments and various treatments. We discover that transition metal vanadium (V) effectively inhibits glucose metabolism in CRC and synergizes with glutaminase inhibitors (BPTES) to disrupt CRC's energy dependency. Thus, a dual energy metabolism suppression nanosystem (VSi-BP@HA) is engineered by loading BPTES into V-doped hollow mesoporous silica nanoparticles. This nanosystem effectively dampens CRC energy metabolism, eradicating 33% of tumors in mice. Strikingly, the cell biological and preclinical model datasets provide compelling evidence showing that VSi-BP@HA not only reverses CRC cells chemo-resistance but also drastically potentiates anti-PD1 immunotherapy. Therefore, this nanosystem provides not only a promising approach to suppress CRC, but also a potential adjunct tool for enhancing chemotherapy and immunotherapy.
研究领域:
肿瘤学
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药理学
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纳米技术
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免疫学
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癌症代谢
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DOI
PubMed
高影响因子
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
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2024
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IF 4.8
An-Qi Zhu,Ning Luo,Xiao-Ting Zhou,Min Yuan,Chu-Mei Zhang,Tian-Ling Pan,Kun-Ping Li
Along with the increasing prevalence of obesity worldwide, the deleterious effects of high-calorie diet are gradually recognized through more and more epidemiological studies. However, the concealed and chronic causality whitewashes its unhealthy character. Given an ingenious mechanism orchestrates the metabolic adaptation to high-fat high-fructose (HFF) diet and connive its lipotoxicity, in this study, an experimental rat/mouse model of obesity was induced and a comparative transcriptomic analysis was performed to probe the mystery. Our results demonstrated that HFF diet consumption altered the transcriptomic pattern as well as different high-calorie diet fed rat/mouse manifested distinct hepatic transcriptome. Validation with RT-qPCR and Western blotting confirmed that SREBP1-FASN involved in de novo lipogenesis partly mediated metabolic self-adaption. Moreover, hepatic ACSL1-CPT1A-CPT2 pathway involved in fatty acids β-oxidation, played a key role in the metabolic adaption to HFF. Collectively, our findings enrich the knowledge of the chronic adaptation mechanisms and also shed light on future investigations. Meanwhile, our results also suggest that efforts to restore the fatty acids metabolic fate could be a promising avenue to fight against obesity and associated steatosis and insulin resistance challenged by HFF diet.
研究领域:
营养学
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分子生物学
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代谢学
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肥胖研究
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DOI
PubMed
高影响因子
Nature Microbiology
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2025
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IF 19.4
Yuan Chen,Hullahalli Karthik,Huang Hao,Zhao Siqi,Wang Wenqing,Tian Xingyu,Li Xin,Xia Linya,Wang Yuchang,Pan Fei,Liang Ying,Xie Yurui,Li Yue,Fan Hongjie,Waldor Matthew K.,Ma Zhe
Proliferation of the emerging zoonotic pathogen Streptococcus equi subsp. zooepidemicus in the meninges is linked to mortality in pigs and morbidity in humans. The mechanisms underlying the remarkable capacity of hypervirulent S. zooepidemicus to proliferate in cerebrospinal fluid (CSF) are largely undefined. Here, using genetically barcoded S. zooepidemicus , we found that following systemic infection of mice, only ~1–10 S. zooepidemicus clones invade the meninges where they subsequently replicate ~10 7 -fold. Subsequent transposon insertion sequencing experiments, plus validation work with bacterial mannose phosphotransferase system (PTS man )-defective strains, identified the PTS man , which imports glucose, as essential for S. zooepidemicus proliferation in CSF. The S. zooepidemicus PTS man promoter confers species-specific constitutive transcription of PTS man , enabling glucose acquisition at low glucose concentrations and limiting activation of the stringent response, leading to pathogen replication in CSF. Our findings reveal how the rewiring of PTS man in the control of S. zooepidemicus metabolism enables this pathogen to adapt to and replicate in CSF during meningitis.
研究领域:
微生物学
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传染病学
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细菌致病机制
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代谢调控
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产品关联
DOI
PubMed
高影响因子
Water
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2025
·Vol. 17
·期 17
·2611
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IF 3
Keying Sun,Huining Zhang,Kefeng Zhang,Jianqing Ma,Zhengmin Pan,Shuting Zhang
The anaerobic ammonium oxidation (anammox) process offers potential for saline wastewater treatment but is hindered by salt inhibition. This study investigates the salt tolerance mechanisms of granular (R1), biofilm-carrier (R2), and floccular (R3) sludge in up-flow anaerobic sludge blanket (UASB) reactors under 0–20 g/L NaCl. Granular sludge outperformed other biomass types, maintaining >90% ammonia nitrogen (NH4+-N) removal at 20 g/L NaCl due to structural stability and extracellular polymeric substances (EPS) adaptation (shift from hydrophobic proteins to polysaccharides). Microbial analysis revealed a transition fromPlanctomycetes/Proteobacteriato salt-tolerantPseudomonadota, withCandidatus_KueneniareplacingCandidatus_Brocadiaas the dominant anaerobic ammonium oxidation bacteria (AnAOB) (reaching 14.5% abundance in R1). Genetic profiling demonstrated coordinated nitrogen metabolism: Hzs/Hdh inhibition (>85%) and NirBD/NrfAH activation (0.23%) elevatedNH4+-N, while NarGIV/NapA decline (1.10%→0.58%) increased nitrate nitrogen (NO3−-N). NxrB/NirSK maintained low nitrite nitrogen (NO2−-N), and GltBD upregulation (0.43%) enhanced osmoregulation. These findings underscore the superior resilience of granular sludge under high salinity, linked to microbial community shifts and metabolic adaptations. This study provides critical insights for optimizing anammox processes in saline environments, emphasizing the importance of biomass morphology and microbial ecology in mitigating salt inhibition.
研究领域:
环境工程
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微生物生态学
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废水处理
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生物技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）