Materials Today Communications
·
2022
·
IF 3.662
Bo Li,Moyuan Qu,Haocheng Yang,Yue Shu,Wenqian Xiao,Songsong Zhu,Xiaoling Liao
Simulating the hierarchical structure of natural bone, mineralized synthetic polymer fibrils such as poly(L-lactic acid) (PLLA) in the form of microspheres show considerable potential as injectable scaffolds for bone defect repair. However, the hydrophobicity of PLLA restricts the deposition of bone-like apatite on the surface of PLLA directly, limiting its application in osteogenesis. In this paper, after sodium trimetaphosphate (STMP) modification, nanofibrous PLLA microspheres were immersed into 2.5 times simulated body fluid (2.5 ×SBF) for 3 days to produce abundant bone-like apatite on the surface of microspheres. The bioactivity in vitro and bone repair ability in vivo of surface mineralized PLLA microspheres were evaluated. The results indicated that surface mineralized nanofibrous PLLA microspheres improved MSCs proliferation and bone-related gene expression, including OPN, RUNX-2 and OCN. After the surface mineralized nanofibrous PLLA microspheres were implanted into rat skulls to repair bone defects (5 mm) for 6 weeks, the micro-CT and histology results showed that the surface mineralized nanofibrous PLLA microspheres improved bone regeneration compared to pure nanofibrous PLLA microspheres. Our study provides a feasible strategy for the rapid preparation of biomimetic mineralized PLLA scaffolds, the novel mineralized microspheres showed considerable bone repair potential.
研究领域:
生物材料学
·
组织工程学
·
医学
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Yang Peng,Aosong Deng,Xun Gong,Xiaomin Li,Yang Zhang
Considering the high concentration of mercury in industrial wastewater, such as coal-fired power plants and gold mining wastewater, this research study investigated the coupling process of lipid production and mercury bioremediation using microalgae cells. Chlorella vulgaris modified by biomimetic mineralization. The cultivation was divided in two stages: a natural cultivation for 7 days and 5 days of Hg 2+ addition (10–100 μg/L) for cultivation at different pH values (4–7) after inoculation. Next, the harvested cells were eluted, and lipid was extracted. The fluorescein diacetate (FDA) dye tests demonstrated that the mineralized layer enhanced the biological activity of microalgae cells in Hg 2+ contaminated media. Hg distribution tests showed that the Hg removal capacity of modified cells was increased from 62.85% to 94.74%, and 88.72% of eluted Hg 2+ concentration was observed in modified cells compared to 48.42% of raw cells, implying that more mercury was transferred from lipid and residuals into elutable forms.
研究领域:
环境生物学
·
微生物学
✓ 已验证
DOI
高影响因子
BMC Oral Health
·
2025
·Vol. 25
·期 1
·1-15
·
IF 3.1
Li Huanying,Liu Gengbo,Liu Xiaohao,Wu Buling
Section
Background
Currently, many biomimetic mineralization materials fail to bind effectively to collagen, which significantly limits their remineralization efficacy. To address this limitation, we designed MMP2-derived peptides (MDPs) on the basis of the strong affinity domain of collagen: MDP-a and MDP-3DSS.
Section
Methods
Surface plasmon resonance (SPR) was used to measure the binding force of MDPs to collagen. Inductively coupled plasma-mass spectrometer (ICP-MS) was used to evaluate the effects of peptides on the adsorption of calcium ions by collagen. Transmission electron microscopy (TEM) and thermogravimetric analysis (TGA) were employed to analyze the stabilizing effect of MDPs on amorphous calcium phosphate (ACP) and the mineralization effect on collagen. Scanning electron microscopy (SEM), mechanical tests, and the CCK-8 assay were employed to evaluate the remineralization effect of MDPs on demineralized dentin and their biocompatibility.
Section
Results
The results of the present study demonstrated that MDPs strongly bind to collagen (18 µM for MDP-a and 18.9 µM for MDP-3DSS) and can promote calcium ion adsorption by collagen (p < 0.0001). Furthermore, MDPs have been shown to stabilize ACP and induce intrafibrillar mineralization. In vitro studies revealed that both surface and cross-sectional dentin slices treated with MDPs presented dense mineral deposition. This deposition effectively occluded the dentinal tubules and resulted in enhanced mechanical properties. The tubule plugging rate, the elastic modulus and microhardness of the MDPs group were greater than those of the control group (p < 0.01). Moreover, MDP-3DSS demonstrated the most significant remineralization potential. Additionally, MDPs treatment did not affect the activity of human dental pulp stem cells (hDPSCs) (p > 0.05).
Section
Conclusion s
The present study successfully developed and evaluated two novel biomimetic mineralized peptides, MDP-a and MDP-3DSS. It is necessary to conduct further research to explore the possibility of clinical transformation of MDPs.
研究领域:
口腔生物学
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生物材料学
·
牙体牙髓病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Na Wang,Xiang Zhou,Youming Dong,Ying Zhang,Rong Liu,Haijiao Kang,Kaili Wang,Jianzhang Li
Natural mildew preventives, like citral, have great potential to protect bamboo from mildew, while their long-lasting antimildew performance is yet to be challenged owing to their oxidability and volatility. Inspired by biomimetic mineralization, in this work, citral@ZIF-67 particles were synthesized by encapsulating citral into a zeolite imidazolate framework-67 (ZIF-67) cage and introduced into a bamboo structure. Benefiting from the unique cage structure and large surface area of ZIF-67, it can encapsulate sufficient citral molecules. The encapsulation reduced the particle size of ZIF-67 due to the nucleation of citral. Furthermore, the surface area, pore size, and volume of citral@ZIF-67 particles gradually decreased with the increase in loading amounts of citral. ZIF-67 exhibited a certain antibacterial ability against E. coli and S. aureus. The encapsulation of citral can further enhance the antibacterial activity of ZIF-67. In addition, the citral@ZIF-67 showed slow-release performance that followed the Fickian diffusion mechanism. After the citral@ZIF-67 treatment, bamboo showed no mold spots on the surface with an infection grade of 0, which was retained, even though bamboo was conditioned at 50 °C and 85% relative humidity for 10 days. This proposed method can effectively improve the stability of natural mildew preventives and endow bamboo with long-term antimildew properties with low cost and minimized environmental impacts.
研究领域:
材料科学
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生物学
·
林业科学
✓ 已验证
产品关联
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Jianhui Li,Qihang Wang,Zhengyi Fu,Zhaoyong Zou
Biomimetic mineralization can facilitate the fabrication of materials with intricate hierarchical structures and distinctive functional properties under ambient conditions. However, precise replication of the complex biomineralization process in vitro remains a big challenge. In this study, aimed at mimicking the biomineralization process of calcite prismatic layers of mollusk shells, a peristaltic pump was employed to slowly transport a sodium carbonate solution into a calcium-containing solution in the presence of poly(acrylic acid) (PAA). Through delicate control of the ion transport rate and reaction conditions, a calcite seed layer was first deposited on the chitosan-coated substrate, followed by the fabrication of a continuous, dense, and highly oriented shell-like calcite prismatic structure. The influences of PAA concentration and ion transportation rate on the growth kinetics and morphology of calcite were studied, showing that optimal conditions are required to form the prismatic structure. It was demonstrated that the prismatic structure started from random formation of an individual spherical disk on the seed layer, which grew larger in diameter and fused with adjacent disks. Furthermore, using confocal Raman mapping analysis, the orientation of calcite crystals at different growth stages was revealed. This study provides a novel bioprocessing-inspired strategy to fabricate a uniform calcite prismatic structure and significantly improves our understanding of the growth mechanisms.
研究领域:
材料科学
·
生物矿化
·
仿生学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Qian Zhou,Jingjing Yuan,Yalin Wang,Ping Wang,Jiugang Yuan,Chao Deng,Qiang Wang
Silk fibroin (SF) has potential applications in the biomedical field because of its excellent mechanical properties and biocompatibility. In the current study, chitooligosaccharide (COS) was enzymatically grafted onto SF using laccase . Subsequently, COS-grafted SF (SF-g-COS) was treated enzymatically in the presence of hexokinase and Mg-chelated adenosine triphosphate (ATP), so as to introduce phosphate groups onto the fibroin chains and promote the deposition of hydroxyapatite (HAp) during in situ biomimetic mineralization . The efficacy of phosphorylation and biomimetic mineralization of the SF-g-COS was evaluated by means of HPLC , MALDI-TOF MS, FTIR , XRD and EDS-Mapping. The results indicate that hexokinase has the capability to catalyze the phosphorylation of COS, resulting in an increase in the quantity of phosphorus in the SF-g-COS. Following mineralization of the phosphorylated SF-g-COS, a greater number of mineral phases were detected on its surface, accompanied by a higher content of calcium and phosphorus compared with other specimens. Cell viability tests using NIH/3T3 cells and cellular adhesion potential with MG-63 cells indicated that the fibroin-based biocomposite exhibited acceptable biocompatibility and superior cellular adhesion properties. The present study describes a novel method for preparation of fibroin/HAp biocomposites for bone tissue engineering.
研究领域:
生物材料学
·
骨组织工程
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2022
·
IF 5.395
Yuxin Wang,Daliang Zhong,Fan Xie,Siying Chen,Zaiqiang Ma,Xinyan Yang,M. Zubair Iqbal,Quan Zhang,Jiaju Lu,Shibo Wang,Ruibo Zhao,Xiangdong Kong
Inorganic nanomaterials showed great potential as drug carriers for chemotherapeutics molecules due to their biocompatible physical and chemical properties. A manganese-based inorganic nanomaterial manganese phosphate (MnP) had become a new drug carrier in cancer therapy. However, the approach for manganese phosphate preparation and drug integration is still confined in complex methods. Inspired by mimetic mineralization, we proposed a “one-step” method for the preparation of manganese phosphate-doxorubicin (DOX) nanomedicines (MnP-DOX) by manganese ion and DOX complexation. The structural characterization results revealed that the prepared MnP-DOX nanocomplexes were homogeneous with controlled sizes and shapes. More importantly, the MnP-DOX nanocomposites could significantly induce cancer inhibition in vitro and in vivo. The results indicated that the drug molecules were integrated into MnP nanocarriers by mimetic mineralization, which not only prevented the premature release of the drug but also reduced excessive modification. Moreover, the designed MnP-DOX complex showed high loading efficacy and pH-dependent degradation leading to drug release, achieving high efficiency for cancer chemotherapy in vitro and in vivo via a facile process. These achievements presented an approach to construct the manganese phosphate-based chemotherapy nanomedicines by mimetic mineralization for cancer therapy.
研究领域:
药学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2020
·
IF 2.627
Xiao Liang,Qing Li,Zhiyuan Shi,Shaowei Bai,Quanshun Li
Enzyme immobilization has been accepted as an efficient technique for improving the stability and recyclability of enzymes. Herein, biomimetic mineralization strategy was employed to achieve the immobilization of urease in a type of metal–organic frameworks (zeolite imidazolate framework-8, ZIF-8), and the immobilized enzyme urease@ZIF-8 was systematically evaluated for its structure, activity, stability and recyclability, using the hydrolysis of urea as a model. The entrapment of urease was found to be realized in a synchronous manner with the formation of ZIF-8 crystal. The loading of urease in ZIF-8 was measured to be ca. 10.6% through the bicinchoninic acid (BCA) protein assay. The encapsulated urease could efficiently maintain its native conformation, which endowed the immobilized urease with excellent activity and stability, even in harsh conditions ( e.g. , in the presence of trypsin, acidic or alkali conditions, or at high temperature). Further, urease@ZIF-8 exhibited good recyclability during the degradation of urea, in which it could keep 58.86% of initial activity after being used for 5 cycles. Thus, biomimetic mineralization could be potentially utilized as a promising method to prepare immobilized ureases with superior activity, stability and recyclability, thereby facilitating the construction of efficient catalysts for industrial biocatalysis and biosensing.
研究领域:
生物化学
·
酶工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Zhenhao Sun,Jia Chen,Ziyou Ding,Yiran Fan,Yingchao Han
The aggregating behavior of hydroxyapatite (HAP) nanorods plays a central role in the mechanism of biomimetic mineralization. In this study, the spindle-like aggregating behavior of hydroxyapatite (HAP) nanorods was studied by ultrasonically dispersing HAP in a polyacrylic acid (PAA) aqueous system. Unlike the quick aggregation and precipitation of HAP nanorods without PAA, the spindle-like primary aggregations formed herein could be suspended in water with good stability. The preferential adsorption of PAA on the side surface of HAP nanorods generated steric repulsion opposite to the van der Waals interaction in the radial direction. Accordingly, with an increase in the molar ratio of PAA[-COOH-]/HAP, the HAP nanorods showed a tendency to form spindle-like primary aggregations with decreased diameter and increased aspect ratio. The resulting aggregates possessed sufficient repulsion (steric repulsion and electric double layer repulsion) to overcome van der Waals interactions to remain suspended and dispersed. This study revealed the mechanism of aggregating HAP nanorods into spindle-like aggregates by extended Derjaguin–Landau–Verwey–Overbeek theory, including the steric stabilization mode. We believe that this study will facilitate the ordered structuring of minerals in hard-tissue mineralization processes.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2021
·
IF 6.789
Ting Yu,Hengyan Ma,Hui Zhang,Minghua Xiong,Yuan Liu,Feng Li
The fragility and high cost of enzymes represent critical challenges limiting their practical application in the removal of pesticides. Herein, an aryloxyphenoxypropionate herbicide-hydrolyzing enzyme, QpeH, was purified via one-step affinity chromatography and embedded into two types of zeolitic imidazolate frameworks (ZIFs) through biomimetic mineralization . The catalytic activity towards the herbicide quizalofop-P-ethyl, the loading capacity and efficiency of the resulting two composites, QpeH@ZIF-10 with cruciate flower-like morphology and QpeH@ZIF-8 with rhombic dodecahedral morphology, were compared. Both composites had excellent stability and reusability after 10 reuse cycles, with QpeH@ZIF-10 having a better performance. More importantly, when applied for the removal of quizalofop-P-ethyl pollution in the watermelon field, QpeH@ZIF-10 (88%) showed a remarkably improved degradation efficiency compared to QpeH@ZIF-8 (84%) despite the latter having a greater loading capacity. Finally, the use of QpeH@ZIF composites was shown to recover the bacterial community in soil. This work provides a new insight into the low-cost synthesis of nanobiocatalysts combining simple purified enzymes and metal-organic frameworks (MOFs) for the remediation of pesticide-contaminated soils.
研究领域:
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
Chemistry-An Asian Journal
·
2016
·Vol. 11
·期 6
·893-899
·
IF 4.592
Yang Yang,Chen Sun,Hin-Lap Yip,Runcang Sun,Xiaohui Wang
Graphical Crystal palace : Perovskite crystals with controlled morphology and properties are formed in a chitosan template by three steps including electrostatic adsorption/chelation, nucleation, and growth. The exposed functional groups of chitosan coordinate strongly with Pb 2+ and some of these stabilized Pb 2+ ions interact with I − ; nucleation occurs and perovskite crystals start to grow in the chitosan network. Biomimetic mineralization is a powerful approach for the synthesis of advanced composite materials with hierarchical organization and controlled structure. Herein, chitosan was introduced into a perovskite precursor solution as a biopolymer additive to control the crystallization and to improve the morphology and film-forming properties of a perovskite film by way of biomineralization. The biopolymer additive was able to control the size and morphology of the perovskite crystals and helped to form smooth films. The mechanism of chitosan-mediated nucleation and growth of the perovskite crystals was explored. As a possible application, the chitosan–perovskite composite film was introduced into a planar heterojunction solar cell and increased power conversion efficiency relative to that observed for the pristine perovskite film was achieved. The biomimetic mineralization method proposed in this study provides an alternative way of preparing perovskite crystals with well-controlled morphology and properties and extends the applications of perovskite crystals in photoelectronic fields, including planar-heterojunction solar cells.
研究领域:
材料科学
·
生物化学
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Leiqing Zhang,Zihuai Zhou,Zhiwen Liu,Ying Shi,Yingjie Mao,Baiping Fu,Zhe Wang
Alendronate (ALN), a potent bisphosphonate, plays a significant role in both bone formation and osteoporosis therapy. However, its potential to promote biomimetic mineralization of type I collagen fibrils and demineralized dentin remains unclear. This study reveals that ALN-pretreated collagen fibrils form ester bonds between the hydroxyl groups of ALN and the carboxyl groups of collagen, accompanied by a reduction in collagen surface potential. Additionally, an adsorption equilibrium of ALN to collagen was achieved at 25 mM ALN. ALN pretreatment facilitates intrafibrillar mineralization of type I collagen fibrils in a dose-dependent manner, as well as mineralization of demineralized dentin in synergy with polyelectrolyte-stabilized Amorphous Calcium Phosphate (ACP) nanoparticles. This process effectively restores the mechanical properties of demineralized dentin to levels comparable to natural dentin. Thus, ALN holds potential for localized applications aimed at promoting remineralization of demineralized dentin.
研究领域:
口腔医学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）