Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Longgang Wang,Yanshuai Cui,Shengfu Chen,Guangzhi Wang,Dawei Gao,Yanping Liu,Qiaoyue Luo,Zhiwei Liu,Xuwu Zhang
The dendrimer based synthetic mimetic enzyme has been drawing great attention. However, this mimetic enzyme is different from the natural enzymes, which are pH sensitive, biocompatible and keep their catalytic activity in biological complex medium. A single zwitterionic layer composed by primary amine and carboxyl groups may be a useful method to obtain these properties. Herein, we report a novel facile method to prepare a mimetic enzyme. The complexes of generation 5 poly(amido amine) dendrimers (G5 PAMAM) with free hemin (G5Hs) were modified by the maleic anhydride and cysteamine. Results showed that the mimetic enzymes (G5HMCs) had pH sensitivity and good stability by varying the pH from 4 to 9, while significant precipitation was observed for free hemin at pH 5 after two days. The G5HMC (3:1) showed optimal catalytic activity at its isoelectric point. Furthermore, G5HMCs displayed excellent biocompatibility. The G5HMCs incubated with fibrinogen were stable for 24 h, while G5Hs immediately formed large aggregates. G5HMC (3:1 2 mg/mL) displayed little cytotoxicity with HeLa cells or A549 cells for 24 h, while G5H (3:1) had serious cytotoxicity, which was also demonstrated by cell morphology observation. At last, G5HMCs fully preserved their catalytic activity in bovine serum albumin (BSA) solution compared with phosphate buffer saline (PBS) solution, while hemin decreased to 73.5–81.5% catalytic activity in BSA solution, which was caused by the less interaction with BSA for G5HMCs than free hemin. The surface functionalization schemes described in this report would represent a versatile method to prepare water-soluble, pH sensitive, biocompatible, and efficient artificial enzymes for biomedical related applications.
研究领域:
生物化学
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纳米医学
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材料科学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Yiqian Su,Kailin Wen,Zhi Yang,Pengcheng Lin
Glutathione (GSH) is an important biochemical substance in the body, and efficient detection plays an important role in evaluating human health. This work develops a simple and efficient method for GSH detection by biomimetic enzyme-catalyzed colorimetric sensing. A novel scandium oxide crystal is prepared by a one-step pyrolysis method. The crystal structure, microstructure, and surface potential of scandium oxide crystals are analyzed by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and potentiometry. Furthermore, the scandium oxide crystal can catalyze the oxidation of the colorimetric substrate TMB in the presence of H2O2, exhibiting characteristics of peroxidase-like activity. Its catalytic effect is influenced by the concentration, temperature, pH, and reaction time, showing a certain degree of dependency. The Michaelis constants of scandium oxide crystal are 0.073 and 0.301 mM for H2O2 and TMB, respectively. Finally, an enzymatic colorimetric sensing method based on the scandium oxide crystal is established for the detection of GSH levels in human serum. The corresponding detection limit and linear range are 0.21 and 0.5–50 μM, respectively. This work provides new insights for developing detection materials and sensing methods toward GSH analysis.
研究领域:
生物化学
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材料科学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3145-3151
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IF 4.58
Wang Lei,Yang Wen,Li Tengfei,Li Duo,Cui Zhimin,Wang Yan,Ji Shunli,Song Qinxin,Shu Chang,Ding Li
The article describes a colorimetric assay for the determination of thrombin. It is based on the application of a triple enzyme-mimetic activity and a dual aptamer binding strategy. The triple signal amplification relies on oxidation of the chromogenic enzyme substrate 3,3,5,5-tetramethylbenzidine (TMB) that is catalyzed by composites consisting of graphene oxide (GO), gold/platinum nanoparticles (AuPtNP), and aptamer (Apt15), a G-quadruplex/hemin conjugate. The dual-aptamer target binding strategy is based on the fact that thrombin has two active sites to be recognized by its aptamers (Apt15 and Apt29). Magnetic beads (MBs) were modified with Apt29 (Apt29-MB) and then are bound by the GO-AuPtNP-Apt15/G-quadruplex/hemin composites. In the presence of thrombin, Apt29-MB and the GO-AuPtNP-Apt15/G-quadruplex/hemin composites form a sandwich-like superstructure. Thus, the absorbance increases due to the formation of TMB oxide produced by catalysis of the composites. Under optimized conditions, the absorbance at 450 nm increases linearly in the 0.30 to 100 nM thrombin concentration range, and the limit of detection is 0.15 nM. The method is simple, rapid, and does not require complicated instrumentation. Bovine serum albumin, human serum albumin and other proteins were found not to interfere.
Schematic presentation of the photometric thrombin assay based on a triple enzyme-mimetic activity of combined nanomaterials (consisting of GO, AuPtNPs and the G-quadruplex/hemin DNAzyme) and two aptamers TMB: 3,3,5,5-tetramethylbenzidine, TMB ox : 3,3,5,5-tetramethylbenzidine oxide, AuPtNP: gold/platinum nanoparticles).
研究领域:
生物化学
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医学检测
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纳米生物学
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DOI
高影响因子
CATALYSIS LETTERS
·
2021
·Vol. 152
·期 8
·2375-2385
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IF 3.186
Li Ying,Wang Qian,Ding Zhu,Wan Dong,Nie Xuetong,Zhong Chongli
Abstract A facile one-step route was presented to prepared NH 2 -MIL-88B(Fe) and Fe 3 O 4 modified reduced graphene oxide nanoparticles (NH 2 -MIL-88B(Fe)@MRGO) as mimic enzymes. The as-prepared NH 2 -MIL-88B(Fe)@MRGO exhibits a higher affinity for hydrogen peroxide and thus possessed a prominent peroxidase-mimic activity. Under the most favorable conditions, the hybrid nanomaterials with high catalytic velocity (2.57 × 10 −7 M s −1 ) and affinity (Km = 0.0091 mM) for substrates of H 2 O 2 display a great glucose detection performance in the range of 20–800 μM with a Limit of detection (LOD) of 3.16 μM (S/N = 3). Graphic Abstract Favorable mimic enzymes catalysis is achieved on NH 2 -MIL-88B(Fe) and Fe 3 O 4 modified reduced graphene oxide nanoparticles (NH 2 -MIL-88B(Fe)@MRGO) for glucose detection.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xiaoli Xie,Dong Ping Wang,Chunxian Guo,Yuhang Liu,Qianghai Rao,Fangming Lou,Qiannan Li,Yongqiang Dong,Qunfang Li,Hong Bin Yang,Fang Xin Hu
Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-C3N4) is prepared by dispersing Ru atoms on a carbon nitride support for the simultaneous electrochemical detection of dopamine (DA) and uric acid (UA), which are coexisting important biological molecules involving in many physiological and pathological aspects. The morphology and elemental states of the single-atom Ru catalyst are studied by transmission electron microscopy, energy dispersive X-ray elemental mapping, high-angle annular dark field–scanning transmission electron microscopy, and high-resolution X-ray photoelectron spectroscopy. Results show that Ru atoms atomically disperse throughout the C3N4 support by Ru–N chemical bonds. The electrochemical characterizations indicate that the Ru-Ala-C3N4 biosensor can simultaneously detect the oxidation of DA and UA with a separation of peak potential of 180 mV with high sensitivity and excellent selectivity. The calibration curves for DA and UA range from 0.06 to 490 and 0.5 to 2135 μM with detection limits of 20 and 170 nM, respectively. Moreover, the biosensor was applied to detect DA and UA in real biological serum samples using the standard addition method with satisfactory results.
研究领域:
生物医学工程
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材料科学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2013
·Vol. 1
·期 34
·4259-4266
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IF 0
Longgang Wang,Qinghua Yang,Guanglong Ma,Weifeng Lin,Zhen Wang,Mei Huang,Shengfu Chen
The dendrimer based synthetic enzyme mimic, so-called ‘dendrizyme’, has been of great interest since the early days of dendrimers. However, there is a lack of an effective way to obtain a natural enzyme mimic showing both good biocompatibility and high preservation of catalytic activity in biological complex medium simultaneously. Here we report a novel approach – the synthesis of a generation five dendrimer of poly(amido amine) (PAMAM) incorporating hemin through capping with carboxybetaine acrylamide (CBAA), which could function as peroxidase. Results showed that the nanocapsules of hemin using CBAA-modified PAMAM dendrimers (CBAA-H-PAMAM) exhibited excellent biocompatibility and full preservation of catalytic activity in bovine serum albumin (BSA) solution, compared with free hemin. Results indicated that the ultra-thin shell of zwitterionic CBAA groups reduced nonspecific interaction with proteins while it did not cause any obvious rise in hindrance to mass transfer. Furthermore, the synthetic peroxidase mimic (CBAA-H-PAMAM) exhibited remarkable temperature endurance as compared with natural proteins. Taken together, our results indicate that protein surface mimicking through CBAA attachment might open a new route for synthetic enzymes in biomedical related applications.
研究领域:
生物化学
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材料科学
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医学工程
✓ 已验证
DOI
PubMed
Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 6
·1169-1177
·
IF 5.068
Ma Yuchan,Lu Mingyue,Deng Yan,Bai Ruyan,Zhang Xi,Li Delei,Zhang Kunlei,Hu Rong,Yang Yunhui
In this work, a nano-mimetic enzyme Co3O4 nanoparticles with catalytic effect on the reduction of H2O2, was synthesized and used to label C-reactive protein antibody. Au nanoparticles (Au NPs) were loaded on COF-TpPa-1 to form Au NPs@COF-TpPa-1
with good biocompatibility and adsorption ability. A sandwich C-reactive protein immunosensor was prepared using Au NPs @COF-TpPa-1 as the immobilization matrix. The catalytic current of Co3O4 nanoparticles to the reduction of H2O2 was measured by
the chronoamperometry which is in proportion to the concentration of CRP. The linear range of this immunosensor was 0.05–80 ng/mL with the linear correlation coefficient of 0.9955. The method demonstrated a good sensitivity with a detection limit of 0.017 ng/mL (S/N = 3) for CRP.
When the C-reactive protein immunosensor was applied to real serum samples analysis, the satisfied results were obtained, indicating that the immunosensor is of prospect of application.
研究领域:
生物医学工程
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2013
·
IF 0
Zujin Yang,Hongbing Ji
2-Hydroxypropyl-β-cyclodextrin was immobilized onto cellulose with epichlorohydrin as a cross-linker. The obtained polymer, as biomimetic catalyst, was used to mediate synthesis of benzaldehyde in aqueous solution. The functionalized mimetic enzyme played a crucial role on catalytic oxidation of cinnamaldehyde with remarkable substrate conversion and product selectivity. Synergistic effect based on weak interactions between the polymer and the substrate results in significant promotion of the catalytic performance. The catalyst could be recycled without apparent loss of the original activity.
研究领域:
生物化学
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催化化学
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产品关联
DOI
RSC Advances
·
2017
·Vol. 7
·期 67
·41989-41999
·
IF 3.108
Dan Su,Hanyun Li,Jinlin Li,Yali Liu,Mi Peng,Bingwei Feng,Pengfei Xu,Yonggui Song
This work reports on a simple, feasible and optical color-enhanced colorimetric immunoassay with hemin (a horseradish peroxidase mimic enzyme) catalyzed color reaction and multifunctional colored silica nanoparticles for qualitative and quantitative determination of avian influenza A (H7N9) virus (H7N9 AIV) at an ultralow concentration by using the magnetic nanobeads (MBs)-mimic enzyme-multifunctional silica nanoparticles color-enhanced chromogenic substrate system. In this work, MBs were fabricated by co-immobilizing target recognition molecules (mAb–MB), which were used as both separation and enrichment carriers. The multifunctional colored silica nanoparticles were synthesized by doping Cy2 (red organic dye) into silica nanoparticles using an inverse microemulsion method, and then made a covalent modification of pAb and glucose oxidase (GOx). In the presence of H7N9 AIV, GOx catalyzed glucose to gluconic acid and hydrogen peroxide (H2O2). The latter can oxidize 4-aminoantipyrine (4-AAP) to red products and the reaction is catalyzed by hemin. The chromogenic compound and the red-silica nanoparticles (Red-SiNPs) together cause the color of the solution to change from colorless to red. Through this color-enhanced immunoassay, we can detect H7N9 AIV with the naked eye and UV detector. And the immunosensor provides a simple and reliable platform with high sensitivity and selectivity which shows great potential in early diagnosis of diseases and in public health security.
研究领域:
医学诊断
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生物化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 9
·2097-2105
·
IF 1.915
Junrong Li,Liang Lv,Guannan Zhang,Xiaodong Zhou,Aiguo Shen,Jiming Hu
We herein present a simple and surfactant-free synthetic strategy for the preparation of core–shell Fructus Broussonetia-like Au@Ag@Pt nanoparticles (FBNPs) via a combination of galvanic replacement and reagent reduction, in which a chitosan (CS)-mediated epitaxial growth of Pt multi-branches occurs on cresyl violet labeled Au core–Ag shell nanoparticles (NPs) at room temperature. The as-prepared novel nanostructures exhibit an enzyme mimetic activity due to the formation of a Fructus Broussonetia-shaped Pt shell, the absence of surfactants and the positively charged CS molecules intertwined within the outermost Pt NPs. Relying on the high sensitivity of resonance-enhanced Raman scattering and the highly efficient intrinsic peroxidase-like activity of FBNPs, H2O2 was detected with a wide detection window from 10 pM to 100 μM when utilizing 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrate. Based on the H2O2–TMB catalytic oxidation system a simple, sensitive, selective and universal platform has been extended for the detection of all peroxidase-related analytes. For example, glucose at a concentration range of 1 nM to 200 μM was detected in the presence of glucose oxidase with a limit of detection of 1 nM.
研究领域:
纳米材料
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生物分析化学
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Quan Wang,Xiaoyu Wang,Hui Wei
Phenol and its derivatives, known as persistent organic pollutants, have long threatened human health and environmental safety. There is an urgent need to develop convenient, low-cost, and multiplex analytical methods. Since phenols are substrates of laccase, they can be detected via laccase-catalyzed colorimetric assays. Nevertheless, the laccase-based assays cannot distinguish different phenols. Moreover, natural laccases suffer from high cost and low stability issues. To meet these needs, here we developed a laccase-like nanozyme sensor array for phenol detection and differentiation, which takes advantage of both nanozymes and cross-reactive sensor arrays. First, we examined a series of spinel-type transition metal oxides and found that manganese on octahedral sites profoundly affects the laccase-like activity of the materials. Based on the developed manganese-based spinel oxides (i.e., Mn3O4, Zn0.4Li0.6Mn2O4, and LiMn2O4), a colorimetric sensor array was constructed. The sensor array could effectively identify and discriminate phenol and its derivatives and showed good performance in the identification and differentiation of phenols in tap water samples. This work provides an important guidance for the development of laccase-like nanozymes and a promising methodology for pollutant monitoring.
研究领域:
环境科学
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生物化学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Ting Chen,Caiyan Lai,He Zhao,Jie Yang,Kai Huang,Xu-Jia Hong,Yuepeng Cai,Renfeng Dong
Green and efficient total antioxidant capacity (TAC) detection is significant for healthy diet and disease prevention. This work first proposed the concept of TAC colorimetric detection based on microrobots. A novel metal-organic framework (MOF)-based biomimetic enzyme microrobot (MIL-88A@Fe 3 O 4 ) is developed that can efficiently and accurately detect the TAC of real fruits and vegetables. Unlike the previous colorimetric detection method to measure TAC which often requires the addition of toxic hydrogen peroxide (H 2 O 2 ) or light, the microrobots strategy can realize efficient TAC detection without any additional chemicals or stimuli. This is attributed to the oxidase-like activity from MIL-88A, which is discovered and confirmed for the first time by experiments and theoretical calculations. In addition, the microrobots can significantly accelerate the color reaction, resulting in a significant improvement in the detection efficiency of TAC in the motion state owing to their self-stirring effect. More importantly, the results of the MOF-based biomimetic enzyme microrobots strategy for detecting TAC in real fruits and vegetables are comparable to those tested by commonly used quantitative detection kits, in addition to low cost, excellent stability, and anti-interference ability. This attractive MOF-based biomimetic enzyme microrobot holds great prospects for future applications in catalytic sensing and promoting a healthy diet.
研究领域:
生物医学工程
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分析化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）