Catalysts
·
2021
·Vol. 11
·期 9
·1083
·
IF 4.146
Hsiang-Ning Luk,Tsong-Yung Chou,Bai-Hao Huang,Yu-Syuan Lin,Hui Li,Ren-Jang Wu
In this study, the Pd/BiVO4composite was prepared by hydrothermal method as an electrochemical sensing material for epinephrine. X-ray diffraction, scanning electron microscopy, and a transmission electron microscope were used to characterize the samples. In the electrochemical detection system, cyclic voltammetry and differential pulse voltammetry were applied to measure the concentration of the epinephrine solution (0.9–27.5 µM) with the Pd/BiVO4-coated glassy carbon electrode. As a result, the oxidation peak current of Pd/BiVO4/GCE demonstrated good linearity with the epinephrine concentration. The detection limit of the epinephrine concentration by cyclic voltammetry and differential pulse voltammetry were 0.262 µM and 0.154 µM, respectively. Additionally, the proposed sensing material exhibited good reproducibility, stability, and selectivity. A plausible sensing mechanism was proposed.Keywords:epinephrine;cyclic voltammetry;differential pulse voltammetry;Pd/BiVO4
研究领域:
生物医学
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材料科学
·
电化学
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 13
·10594
·
IF 5.6
Zanlin Chen,Haiming Li,Miaojia Xie,Fengguang Zhao,Shuangyan Han
This study proposes a label-free aptamer biosensor for the sensitive detection of malachite green(MG) using gold nanoparticles/multi-walled carbon nanotubes @ titanium dioxide(AuNPs/MWCNTs@TiO2). The nanocomposite provides a large surface area and good electrical conductivity, improving current transfer and acting as a platform for aptamer immobilization. The aptamer and the complementary chain(cDNA) are paired by base complementary to form the recognition element and fixed on the AuNPs by sulfhydryl group, which was modified on the cDNA. Since DNA is negatively charged, the redox probe in the electrolyte is less exposed to the electrode surface under the repulsion of the negative charge, resulting in a low-electrical signal level. When MG is present, the aptamer is detached from the cDNA and binds to MG, the DNA on the electrode surface is reduced, and the rejection of the redox probe is weakened, which leads to an enhanced electrical signal and enables the detection of MG concentration by measuring the change in the electrical signal. Under the best experimental conditions, the sensor demonstrates a good linear relationship for the detection of MG from 0.01 to 1000 ng/mL, the limit of detection (LOD)is 8.68 pg/mL. This sensor is stable, specific, and reproducible, allowing for the detection of various small-molecule pollutants by changing the aptamer, providing an effective method for detecting small-molecule pollutants.Keywords:aptamer;cyclic voltammetry;potassium hexacyanoferrate;sensitive detection;differential pulse voltammetry
研究领域:
分析化学
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环境科学
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生物传感器技术
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2017
·
IF 3.012
Changling Yan,Ruixing Zhang,Yujuan Chen,Gongke Wang
A novel molecularly imprinted polymer (MIP), poly(pyrrole- co - o -phenylenediamine) (p(Py- co -OPD)), has been developed in order to construct an enrofloxacin (EF) sensor by using cyclic voltammetry (CV) technique. The preparation conditions including the pH level, EF concentration, the number of CV cycles, extraction solution of the template and the incubation time were optimized. The performance of the MIP sensor was evaluated by square wave voltammetry (SWV). Under the optimal experimental conditions, the MIP sensor exhibited long time stability, good selectivity and a wide linear range of 1.0 × 10 − 4 –1.0 × 10 − 10 mol/L with a low detection limit of 6.57 × 10 − 13 mol/L towards EF. Moreover, the sensor was successfully used to determine the EF amount in the pharmaceutical samples with satisfactory results.
研究领域:
分析化学
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电化学传感器
·
药物分析
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Rui Liu,Xin Zhang,Ri-jia Liu,Yuan Sun,Bin-qiao Ren
In this study, we created a brand-new g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF electrode that can be used as a platform for the electrochemical sensing of Rutin (RT). The g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF composite was synthesized via calcination treatment and electrodeposition, coupled with hydrothermal reaction. This composite could appreciably enlarge the electrochemically active surface area of the as-prepared sensor to promote electron transfer and result in the enhanced response signals.With the assistance of differential pulse voltammetry (DPV) and cyclic voltammetry (CV), the electrochemical performance of sensor can be greatly enhanced. Under these optimum conditions, the designed electrochemical sensor was able to sense RT at the lower detection limit (LOD) of 0.69 nM (S/N = 3) with a wider range of 5–500 nM and showed high sensitivity of 1.97 μA . nM −1. cm −2 at room temperature. Additionally, the selective investigation on RT was performed with the related interfering molecules, and the g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF/GCE showed decent stability and reproducibility. The real-time application of the modified electrode for RT detection was monitored in both pharmaceutical and serum samples with the satisfactory recovery. Overall, this research contributes to the field of the electroanalytical sensing and offers the potential applications in the drug monitoring.
研究领域:
分析化学
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药物监测
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生物医学工程
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yuting Wang,Xiangfei Chen,Dejuan Huang,Tianxiang Jin
Three-dimensional graphene (3DG) is presented as an excellent electrocatalyst for the simultaneous detection of hydroquinone (HQ) and catechol (CC) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Morphology and structure of 3DG were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Compared with the bare glassy carbon electrode (GCE), the 3DG-modified GCE exhibits better electrocatalytic activity in the redox reaction of HQ and CC. The 3DG-modified GCE detects both isomers selectively and sensitively by DPV and CV, latter showing a peak-to-peak separation of 190 mV. The detection limits for HQ and CC can reach 0.35 and 0.6 μM (S/N = 3), respectively, with wide linear ranges of 1-1000 μM in the presence of 50 μM of the respective other isomer. This work provides a strategy for the development of high-performance electrochemical sensors.
研究领域:
材料科学
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分析化学
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电化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2018
·
IF 4.229
Ke Zhang,Xu Zhao,Sihan Chen,Liping Chang,Jiancheng Wang,Weiren Bao,Hui Wang
In chemical cycles to produce hydrogen , the H 2 S splitting cycle and the sulfur-iodine (S I) water splitting cycle both share the Bunsen reaction and HI decomposition. Therefore, they have to overcome the same challenges in the technology development, one of them being the complex and difficult separations of the mixed hydroiodic acid and sulfuric acid solution after the Bunsen reaction. To avoid the separations, this paper studies the electrolysis of the HI/H 2 SO 4 /H 2 O/toluene mixture, focusing on the electrochemical characterization of the Pt electrode by using linear sweep voltammetry (LSV) and cyclic voltammetry (CV). The results show that hydrogen is identified from the gas generated from the cathode in electrolysis. Iodide oxidation is the main reaction in the anode chamber and no significant side reactions are observed. Iodine deposition on the anode surface increases the resistance to iodide diffusion to the anode. However, it can be mitigated by adding toluene in or applying stirring to the anolyte HI/H 2 SO 4 solution. The Pt cathode and sulfuric acid catholyte also work stably.
研究领域:
化学工程
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电化学
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可再生能源
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lin Hu,Xiaoqin Zhou,Jinqing Li,Tongtong Bai,Juan Tang,Tao Zhou,Qujin Cui
In this study, we used ferrocene/graphene to modify a glassy carbon electrode, which was fixed with an appropriate amount of Nafion solution. The -C-O-C- and -COOH functional groups were detected in the ferrocene/graphene composite using IR spectroscopy, which indicated that graphene was oxidized to graphene oxide, making it more hydrophilic, dispersible and compatible with polymers, thus increasing the chloromycetin detection rate. A scan rate of 0.05 V/s is ideal. The optimal conditions for chloromycetin detection were found using Cyclic voltammetry (CV): a scan rate of 0.05 V/s, pH 6.47, and 0.1 mol/L PBS buffer solution. Differential pulse voltammetry(DPV) shows that the concentrations of chloromycetin are 2×10 -6 - 3.2×10 -5 mol/L, and there is a good linear relationship between the peak current and the concentration: I pa (μA)=2.44772 c(μmol/L) -46.14375, r = 0.993; the detection limit is 4.5×10 -8 mol/L. This modified electrode has good stability, repeatability and operability and can be applied to detect actual samples.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Guo Chen,Xia Hao,Bang Lin Li,Hong Qun Luo,Nian Bing Li
In this work, a simple and reliable electrochemical method for the determination of cadmium using an antimony film modified sodium montmorillonite (NaMM) doped carbon paste electrode (Sb/NaMM-CPE) was described. Due to the strong cation exchange ability and adsorptive characteristics of NaMM and excellent electroanalytical performance of the antimony film, the new electrode significantly enhanced the sensitivity for detection of Cd(II) and exhibited superior performance in comparison with the bare carbon paste electrode, antimony film modified carbon paste electrode (Sb/CPE), and NaMM modified carbon paste electrode (NaMM-CPE). Some parameters such as the composition of the paste, pH of the measure solution, the Sb(III) concentration, deposition time, and deposition potential that influenced the response of cadmium were investigated and the procedure was optimized for cadmium determination. This electrode, combined with square-wave anodic stripping voltammetry, displayed excellent linear behavior in the examined concentration range of 4.0–150.0 μg L −1 Cd(II) ( R 2 = 0.998) with a detection limit of 0.25 μg L −1 . The Sb/NaMM-CPE was successfully applied to the determination of cadmium ion in tap water sample.
研究领域:
电化学
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分析化学
·
环境科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yufeng Zhang,Lixia Yang,Chang Sun,Chao Huang,Bixue Zhu,Qianjun Zhang,Dongmei Chen
ABSTRACT: Aptamers are single-stranded DNA or RNA that can specifically bind to a target substance with high affinity. Pesticides have obvious biological effects and are very harmful to the human body and the environment. Surface-enhanced Raman spectroscopy (SERS) can be combined with aptamer for the rapid detection of pesticide residues because of its simple operation and high sensitivity. However, a single analysis method has certain limitations and can't satisfy the research of complex system. In this study, SERS and electrochemical were used to prove the specific recognition of malathion by aptamer. The biochemical sensor directly detected the SERS spectra of the aptamer, malathion, and aptamer-malathion through the SERS method. Malathion could also be verified the specific recognition of aptamer by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Based on the peak difference before and after the combination of malathion and aptamer, a detection method for the specific recognition of malathion in a complex system was established. The identification mechanism was discussed. Then, the practical detection of malathion demonstrated the practicability of the method. The method was applied for the detection of malathion in spiked apple and bell pepper samples. The recoveries were in the range of 89.80%–106.50%, and the relative standard deviations were from 2.51%–6.92%. The use of aptamers for the specific detection of pesticides will have the potential for broad applications in the future.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2024
·
IF 1.3
Ying Wang,Wei He
This study presents a novel electrochemical fingerprinting method for Buzhong Yiqi Tang extract granules using a diamond-graphene composite electrode. The electrode was fabricated through chemical vapor deposition of graphene on high-pressure high-temperature synthetic diamond, resulting in a few-layer graphene coating as confirmed by Raman spectroscopy (I₂ D /I G ratio ≈ 1.2) and XPS analysis (78 % sp² C-C signal). Cyclic voltammetry revealed four anodic and three cathodic peaks, while differential pulse voltammetry provided enhanced resolution with eight distinct oxidation peaks. The method demonstrated high sensitivity (LODs 0.08–0.15 mg/mL) and good linearity (R² > 0.995) over a concentration range of 0.5–10 mg/mL. Comparison of fingerprints from individual herb extracts (Astragalus membranaceus, Panax ginseng, and Glycyrrhiza uralensis) elucidated the origins of major peaks in the Buzhong Yiqi Tang profile. Principal component analysis of fingerprints successfully differentiated between samples from three manufacturers, accounting for 87.3 % of total variance. Strong correlations (r > 0.92) were observed between key DPV peak intensities and HPLC-determined concentrations of traditional chemical markers. The rapid analysis time, minimal sample preparation, and holistic representation of multiple electroactive components make this approach a promising complement to existing chromatographic methods for quality assessment of traditional Chinese medicine formulations.
研究领域:
中药分析
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电化学
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高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-7
·
IF 5.705
Meng Fanyu,Xu Yuanyuan,Dong Wenfei,Tang Yuguo,Miao Peng
The paper describes a voltammetric method for the quantitation of the activity of telomerase extracted from cancer cells. A thiolated single-stranded telomerase substrate primer was firstly immobilized on a gold electrode. In the presence of a mixture of telomerase and deoxynucleotide triphosphates, the primer becomes elongated and contains repetitive nucleotide sequences (TTAGGG) n . After hybridization with blocker DNA, gold nanoparticles are added and captured by the elongated single-stranded DNA. This reduces the charge transfer resistance of the gold electrode. The telomerase activity is then quantified via differential pulse voltammetry, typically at 0.12 V (vs. SCE). The method is PCR-free, rapid, and convenient. It was applied to the detection of HeLa cells via the telomerase activity of lysed cells. The detection range was from 500 to 50,000 cells/mL and the detection limit was as low as 500 cells/mL.
A telomerase substrate (TS) primer is immobilized on a gold electrode as the sensing interface to detect the activity of telomerase extracted from cancer cells. Unmodified gold nanoparticles (AuNPs) are utilized which change the electrochemical responses.
研究领域:
生物化学
·
分析化学
·
癌症研究
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Qian Han,Kangkang He,Ruisheng Han,Jianda Zhang
Electropolymerization of L-lysine incorporating multiwall carbon nanotubes (MWCNTs) dispersed in β -cyclodextrin prepolymer (CDP) solution was constructed a very efficient sensor. Scanning electron microscopy was employed to characterize the surface morphology of the modified electrode. The proposed sensor excellently enhanced the selectivity in the simultaneous determination of HQ and CC by differential pulse voltammetry and cyclic voltammetry. Comparing with bare GCE, MWCNTs/GCE, CDP-MWCNTs/GCE, the introduction of L-lysine polymer film effectively declined the shoulder peak of buffer soultion which seriously interfered the dection of hydroquinone (HQ) and catechol (CC) isomers. The PLL/CDP-MWCNTs/GCE displayed good discrimination ability and high sensitivity toward HQ and CC, peak-to-peak separation about 105 mV were obtained. The linear response range for hydroquinone and catechol were 0.1 μM – 100 μM. The detection limits of HQ and CC were 0.044 μM, 0.058 μM, respectively.
研究领域:
分析化学
·
材料科学
·
环境监测
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DOI
高影响因子
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