JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
·
2021
·
IF 3.849
Jianjun Guo,Xiaoliang Wang,Wenrong Wang,Lingling Jia,Wei Guo,Guohua Wu
Background Phoxim is a widely used organophosphorus pesticide in agriculture. People are paying more and more attention to its toxicity. At present, there is no appropriate way to solve the phoxim poisoning of silkworm , which severely affected the development of sericulture . Fe 2+ , Cu 2+ , Rb + exerted their biological effects through various forms in vivo . Methods To evaluate the effect of Fe 2+ /Cu 2+ /Rb + on phoxim poisoning in silkworm, Bombyx mori were treated with fresh mulberry leaves soaked in 2.5 mg/L phoxim for 2 min with 50 mg/L FeCl 2 , 150 mg/L CuCl 2 , or 0.5 mg/L RbCl from 5 days of the fifth-instar silkworm. Results Fe 2+ , Cu 2+ , and Rb + pretreatments significantly inhibited the phoxim-induced reduction of survival rate and alleviated the phoxim-induced poisoning symptoms. The protective effects of Fe 2+ , Cu 2+ , and Rb + on phoxim poisoning might be due to their enhancement of superoxide dismutase (SOD), catalase (CAT), and carboxylesterase (CarE) in the hemolymph and fat body of silkworm. This enhancement might reduce reactive oxygen species (ROS) accumulation and oxidative stress (OS) caused by phoxim poisoning. Thereby it reduced the damage to silkworm tissues and cells. Conclusions These results showed that Fe 2+ , Cu 2+ , and Rb + treatments protected the silkworm from phoxim poisoning by directly enhancing the activity of SOD, CAT, and CarE enzymes and reducing oxidative stress, but not dependent on the high expression of CYP genes. The use of Fe 2+ , Cu 2+ , and Rb + to enhance the activity of SOD, CAT, and CarE enzymes may be an underlying effective way to solve phoxim poisoning in the silkworm industry.
研究领域:
昆虫学
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毒理学
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农业
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yaqing Zhao,Yaming Sun,Chenchen Song,Hailiang Zhao,Shiqi Chai,Xuemeng Xu,Lijun He
Organophosphorus pesticides (OPPs) severely pollute various environmental water due to their excessive use, and it is extremely urgent to develop novel adsorbents with high adsorption capacities, rapid removal rate and easily recovery for the removal of OPPs. In this study, defect-rich Co/N-doped hierarchically porous carbons (Co/N-DHPCs) were constructed by pyrolyzing acid-etched ZIF-67 precursor. The developed Co/N-DHPCs possessed rich defects, well-developed hierarchical porous structure, high specific surface area and excellent magnetic property, and exhibited large adsorption capacities of 103.81∼126.10 mg g -1 and high removal efficiencies of 94.06 %∼100.00 % for fenitrothion, fenthion and phosalone within 15 min. The exceptional adsorption performance of Co/N-DHPCs could be attributed to the pore-filling effect, hydrophobic and π-π interactions, as well as the new active sites formed by rich defects and N heteroatoms doping. In addition, the magnetic property of Co/N-DHPCs enabled rapid and straightforward separation from aqueous solution. The Co/N-DHPCs also showed good reusability with removal efficiencies of 85.24 % after five consecutive recycles. Furthermore, the developed Co/N-DHPCs demonstrated remarkable removal efficiencies of OPPs in seven environmental water samples, particularly in high-salt seawater. This work provides a kind of reliable adsorbents for removing pollutants in environmental water.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 14
·3423
·
IF 4.2
Kangmiao Guo,Xiaokun Wu,Fan Zhang,Ying Cao,Zenglei Tan,Shuwen Xiao,Lijie Wu
A deep eutectic solvent (DES) with the ability to change from hydrophilic to hydrophobic was designed and synthesized and applied to the determination of organophosphorus (OPP) pesticides in honeysuckle dew samples. Choline chloride, phenol, and tetrahydrofuran (THF) were used as the hydrogen bond acceptor, hydrogen bond donor, and demulsifier, respectively. Eight OPP pesticides were extracted by DES coupled with ultrasonic-assisted extraction (UA) and then chromatographed by GC-MS. DES used as an extract solvent has the advantages of high extraction efficiency, low cost, and environmental protection. Furthermore, DES is compatible with GC-MS. The single factor experiment design and Box–Behnken design (BBD) were applied to the optimization of experimental factors, including the type and composition of extraction solvent, type of demulsifier solvent, the volume of DES and THF, pH of sample solution, and ultrasonic time. Under the optimum experimental conditions, the high degree of linearity from 0.1 to 20.0 ng mL−1(R2≥ 0.9989), the limits of detection from 0.014 to 0.051 ng mL−1(S/N = 3), and the recoveries of analytes from 81.4 to 104.4% with relative standard deviation below 8.6%. In addition, the adsorption mechanism of OPPs on DES was explored by adsorption kinetic studies. These results have demonstrated that the present method has offered an effective, accurate, and sensitive methodology for OPP pesticides in honeysuckle dew samples, and this method provides a reference for the detection of pesticide residues in traditional Chinese medicine.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Linfeng Chen,Xike Tian,Yong Li,Liqiang Lu,Yulun Nie,Yanxin Wang
Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening method based on a high-throughput optical array system assembled by multiple cholinesterases (ChE) and thiocholine (TCh) sensors is described. The detection mechanism is that the inhibition of ChE activity by pesticides reduces the TCh produced by the hydrolysis of butyryl/acetylthiocholine iodide, thus changing the fluorescence intensity of TCh sensor. The diverse response of ChEs to pesticides and different affinity of sensors to TCh ensure the high-throughput and distinguishable signal output, which allow the establishment of high discrete pesticide database with intra-cluster agglomeration and inter-cluster dispersion. By using the database, the screening of unknown real contaminated samples were successfully operated, and the screened pesticide species and concentrations were consistent with high-performance liquid chromatography. This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.
研究领域:
分析化学
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环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qianqian Lu,Lingling Guo,Xinxin Xu,Hua Kuang,Liqiang Liu,Chuanlai Xu,Maozhong Sun
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared. On this basis, a multiplex fluorescent microsphere immunochromatographic assay (FM-ICA) was developed for simultaneous detection of three OPs in cowpea and orange samples. The quantitative detection ranges of PHO, FEN, and PRO were 9.039–140.129, 6.686–108.613, and 5.215–71.479 μg/kg, respectively, in cowpeas; and 7.120–121.566, 3.592–92.185, and 3.579–60.081 μg/kg, respectively, in oranges. The results of recovery and real sample testing showed that the multiplex FM-ICA was rapid and accurate, making it suitable for the on-site testing of a large number of samples.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 11
·3161-3169
·
IF 2.245
Kang Yan,Li Long,Chen Wanchao,Zhang Feiyu,Du Yiping,Wu Ting
A structure of polyurethane micelle/Ag nanoparticle (Ag NP) cluster was fabricated as surface-enhanced Raman scattering (SERS) substrate for in situ extracting organic compounds on the surface of different matrixes, in which polyurethane micelles acted as the host material to capture target molecules and stabilize nanoparticle cluster. This method provided stable aqueous suspensions of Ag NP cluster due to the amphiphilic properties of polyurethane. We demonstrated SERS-based in situ detection of pesticides on vegetables and fruit skin, by simply dropping polyurethane micelle/Ag NPs substrate on the sample surface where the target molecules could be detected without a previous elution and extracting. The obtained results showed that this polyurethane micelle/Ag NP cluster substrate had excellent SERS performance for pesticide molecules with ideal stability and reproducibility. With further optimization, the limit of detection of 0.03 μg/mL acetamiprid, 0.08 μg/mL phosmet, and 0.002 μg/mL thiabendazole was obtained, respectively.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 9
·2094-2105
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IF 2.413
Yaseen Tehseen,Pu Hongbin,Sun Da-Wen
The sensitivity of surface-enhanced Raman spectroscopy (SERS) extremely relies on experimental parameters including pH and aggregating agents. Using Au@Ag nanoparticles (NPs) with 26 nm Au core size and 6 nm Ag shell thickness as the substrate, the effect of cationic (Ca 2+ , K + , Na + ) and anionic (Cl − , Br − ) aggregating agents was investigated on the SERS detection of phosalone. The optimum concentrations of the aggregating agents in relation to the maximum SERS intensity differed broadly from 1 × 10 −2 mol/L for CaBr 2 to 1 × 10 1 mol/L for KCl. Both anions and cations greatly affected the SERS enhancement. With employing Br − as the anion, Ca 2+ and K + showed the maximum SERS intensities for phosalone, while using Ca 2+ as the cation, Br − produced the maximum SERS enhancement. Among these aggregating agent combinations, the maximum SERS enhancement was achieved by employing 1 × 10 −2 mol/L CaBr 2 at pH 3.0. The detection limits of phosalone in standard solution and peach were 0.02 mg/L and 0.2 mg/kg, respectively. This study demonstrated that the Au@Ag NP-based SERS approach could be used as a sensitive, rapid, and simple method for detecting trace contaminants in food matrices.
研究领域:
分析化学
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食品科学
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纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Mei-Lan Du,Cheng Yang,Hai-Long Qian,Xiu-Ping Yan
The wide utilization of organophosphorus pesticides (OPPs) results in a potential threat to ecosystem and human health. The essential extraction process in practical OPPs analysis always suffers from the low efficiency due to the limited accessible active sites and interactions in the adsorbents. Here, we report a rational design and synthesis of a novel hydroxyl-functionalized three-dimensional covalent organic framework (3D COF-OH) named JNU-6 with ordered porous structure and multiple interactions as the adsorbent for rapid and selective dispersive solid phase extraction (DSPE) of OPPs. The 3D COF-OH based DSPE was coupled with gas chromatography-flame thermionic detection (GC-FTD) for the determination of four selected OPPs with low limits of detection of 0.15–0.39 ng mL −1 , wide linear range of 1–1100 ng mL −1 . The developed method gave good precision (relative standard deviation of 1.6–8.5% ( n = 5)) and recoveries of 86–106% for the determination of OPPs in complicated fruit and vegetable samples. This work reveals the high practical potential of functionalized 3D COFs as adsorbents for effective extraction of trace contaminants in complicated samples.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 36
·12966-12974
·
IF 8.067
Cheng Ye,Meng He,Zhengdong Zhu,Xiaoyi Shi,Maofeng Zhang,Zhiyong Bao,Youju Huang,Changlong Jiang,Jianhua Li,Yucheng Wu
The rapid identification and quantitative detection of organophosphorus pesticides on a portable device is of vital importance for food safety evaluation and human health protection. However, conventional strategies for realizing the reliable, portable and sensitive detection of target analytes are still challenging. Here, a novel dual-MOF-induced ZnO@Co3O4 heterostructure modified with AgNPs was designed and fabricated as a surface-enhanced Raman scattering (SERS) sensor for the sensitive identification and quantitative analysis of organophosphorus pesticides. The ZnO@Co3O4 heterojunction induced charge transfer enhancement in ZnO@Co3O4@Ag and achieved a 6.6-fold signal amplification compared to that of ZnO@Ag. The synergetic effect of the ZnO@Co3O4 heterostructure and the plasmonic AgNPs together with the molecular preconcentration of the porous structure enabled it to ultrasensitively detect triazophos (10−9 M), fonofos (10−8 M) and thiram (10−7 M) even on a portable Raman instrument. The SERS sensor exhibited superior signal reproducibility with an RSD value less than 8% and long-term stability with a signal loss of only 8.9% after 30 days. Moreover, this SERS platform provided the rapid quantitative analysis of triazophos, fonofos and thiram residues on real samples of tea and dendrobium leaves with LOD values of 10−7 M, 10−6 M and 10−6 M, respectively. In addition, the multiplex detection of pesticides was realized, indicating that our strategy has great potential for quantitative pesticide residue analysis in real samples.
研究领域:
食品安全
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 5
·560
·
IF 5.4
Tingting Lang,Meiyu Xiao,Wenyang Cen
Organophosphate insecticides with broad spectrum and high efficiency make a great difference to agricultural production. The correct utilization and residue of pesticides have always been important issues of concern, and residual pesticides can accumulate and pass through the environment and food cycle, resulting in safety and health hazards to humans and animals. In particular, current detection methods are often characterized by complex operations or low sensitivity. Fortunately, using monolayer graphene as the sensing interface, the designed graphene-based metamaterial biosensor working in the 0–1 THz frequency range can achieve highly sensitive detection characterized by spectral amplitude changes. Meanwhile, the proposed biosensor has the advantages of easy operation, low cost, and quick detection. Taking phosalone as an example, its molecules can move the Fermi level of graphene with π–π stacking, and the lowest concentration of detection in this experiment is 0.01 μg/mL. This metamaterial biosensor has great potential in detecting trace pesticides, and its application in food hygiene and medicine can provide better detection services.
研究领域:
食品科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）