Biomed Research International
·
2015
·
IF 1.579
Wen-Hao Yu,Na Zhang,Jin-Feng Qi,Chen Sun,Yong-Hui Wang,Mei Lin
Toxic heavy metals, including mercury (Hg) and arsenic (As), accumulate preferentially in kidneys and always cause acute renal failure. The aim of this study was to investigate whether these samples affect organic anion transporters, Oat1 and Oat3, in vivo in mice kidney. Mice () were orally treated with investigational samples. After last administration, all mice were i.v. p-aminohippuric acid (PAH), and the blood and kidneys samples were collected. The concentrations of PAH were quantified by spectrophotometry. mRNA expressions of Oat1 and Oat3 were assayed by real-time PCR. In comparison with corresponding control, major pharmacokinetic parameters of PAH in sera were significantly changed by investigational samples (), PAH accumulations in the kidney tissues were significantly higher (), PAH uptake by renal slices was greatly reduced, Oat1 and Oat3 mRNA expression were significantly inhibited in investigational sample groups. Arsenic and mercury containing traditional Chinese medicine (Realgar and Cinnabar) probably induce kidney damage through inhibiting several members of the organic anion transporters (such as OAT1 and OAT3).
研究领域:
药理学
·
毒理学
·
肾脏病学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2021
·
IF 2.495
Yinyin Wang,Yun Ai,Yilin Zhang,Yuanyuan Ren,Jilin Wang,Fangyuan Yao,Wenyu Li,Ying Zhou,Yanni Sun,Jianli Liu,Cuiling Wang
A new probe TR-V (Indolo[2,1–b]quinazoline-6,12-dione,6-{(6Z),(2′E)-2′- [(2-hydroxy-3-methoxyphenyl)methylene]hydra zinylidene}) based on tryptanthrin has been synthesized for selective and rapid detection of Cu 2+ . The synthetic route is facile, economical and viable. TR-V reacts in Tris-HCl buffer solution (pH = 8.0) with cupric to a deep yellow chelate which has a significant peak at 450 nm in the UV–Vis spectra. The reaction is instantaneous, ranging from light yellow to dark yellow and the change in color can be observed with the naked eye. Over 13 competitive metal ions do not interfere in the determination. The detection limit is 84 nM (R 2 = 0.9998). Thus, this method was selected for the analysis of copper ions in tap, bottled water and traditional Chinese medicine samples under optimized experimental conditions.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Huimin Zhao,Aiping Yang,Nan Zhang,Shiyang Li,Tianjie Yuan,Ning Ding,Siwang Zhang,Sheng Bao,Chang Wang,Yinan Zhang,Xiachang Wang,Lihong Hu
The discovery of new, safe, and effective pesticides is one of the main means for modern crop protection and parasitic disease control. During the search for new insecticidal secondary metabolites from endophytes in Stemona sessilifolia (a traditional Chinese medicine with a long history as an insecticide), 10 new insecticidal endostemonines A–J (1–10) were identified from an endophytic Streptomyces sp. BS-1. Their structures were determined by comprehensive spectroscopic analysis. Endostemonines A–J represent the first reported naturally occurring pyrrole-2-carboxylic ester derivatives, which consisted of different fatty acid chains at the C-2 of pyrrole ring were produced by traditional Chinese medicine endophytic microbes. All new tested compounds exhibited strong lethal activity against Aphis gossypii (LC50 value range of 3.55–32.00 mg/L after 72 h). This research highlighted the discovery of pesticide natural products from insecticidal medicinal plant endophytes for the first time, paving a new pathway for the development of pest control.
研究领域:
植物学
·
农药开发
·
微生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 13
·16655-16662
·
IF 4.223
Xiao Jinjing,Ma Jinjuan,Wang Fan,Xu Xing,Liao Min,Shi Yanhong,Cao Haiqun
Numerous natural preparations in traditional Chinese medicine are prepared as decoctions. Processing factors (PFs) comparing the levels of pesticide residues in decoctions to those in the corresponding unprocessed products should be considered in exposure assessments. Thus, this study determined the residue levels of six pesticides (chlorpyrifos, phoxim, imidacloprid, thiamethoxam, fenpropathrin, and emamectin benzoate), as well as 3,5,6-trichloropyridinol, the primary metabolite of chlorpyrifos, and clothianidin, the main metabolite of thiamethoxam in Baishao, Paeoniae radix lactiflora (Fam. Ranunculaceae). The results showed that significant time-response effects were present for the release of pesticides from P. radix. The PFs calculated were < 1, indicating a significant reduction in pesticide residues after TCM processing. The water solubility and partition coefficient values of the pesticides may have played a basic role in the dissipation of the residues during the TCM decocting process. A risk assessment based on the hazard quotient with PFs revealed that exposure to pesticide residues in P. radix was far below the levels that might pose a health risk. In conclusion, the results presented here are of theoretical and practical value for the safety evaluation of TCMs.
研究领域:
药理学
·
毒理学
·
中医药学
✓ 已验证
DOI
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Shuang Wu,Yansong Zhang,Shengnan Kong,Haiyang Yan,Chuanrun Li,Huchuan Wang
s In this paper, a hybrid system of ultrafiltration and membrane distillation was used to study the resourceful reuse of active compounds and water resources in traditional Chinese medicine wastewater. Pre-treatment of turbid wastewater by ultrafiltration (UF) was found to maximise the retention of macromolecular organic matter in the wastewater using a 10 kDa ultrafiltration membrane at a trans-membrane pressure of 0.15 MPa. The UF filtrate was subjected to vacuum membrane distillation (VMD) at 60 °C, 95 kPa, 36 L·h −1 , and pH = 3, which ultimately recovered about 85 % of chlorogenic acid as well as about 74 % of baicalin. The UF-MD system can effectively separate the large organic molecules, small molecules of active compounds and water in the turbid wastewater of traditional Chinese medicine, so as to realise the recovery and reuse of valuable resources in the turbid wastewater of traditional Chinese medicine.
研究领域:
环境科学
·
中药学
·
水资源管理
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Liu Qian-Bao,Lu Jing-Guang,Jiang Zhi-Hong,Zhang Wei,Li Wen-Jia,Qian Zheng-Ming,Bai Li-Ping
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a sensitive surface analytical technology, which can simultaneously acquire diverse chemical components and their precise locations on the surfaces of samples without any requirements for chemical damage pretreatments or additional matrices. Commonly, the quality control of TCMs (traditional Chinese medicines) is limited by the qualitative and quantitative evaluations of the specifically extractive constituents. In this study, a practical sample preparation strategy named two-layered media embedding sample preparation was developed to obtain ideal freezing sections of dried materials of Cordyceps sinensis. Meanwhile, the well-established sample preparation method was applied for in situ chemical profiling and imaging of natural (NCS) and cultured Cordyceps sinensis (CCS) by using TOF-SIMS. More than 200 components were tentatively identified and imaged in NCS and CCS at the same time. Mass spectrometry imaging revealed that most components have even distributions in caterpillars of Cordyceps sinensis, while TAGs, DAGs, MAGs, and FAs only have distributions outside caterpillars’ digestive chambers. This is the first time that components were in situ imaged for Cordyceps sinensis to exhibit the chemical distributions which have never been achieved by other analytical techniques so far. In addition, chemometrics was used to simplify and explain the massive TOF-SIMS mass data sets, which revealed the high chemical similarity between CCS and NCS. Furthermore, the relative quantification of TOF-SIMS data showed that CCS has comparable proportions of amino acids, nucleosides, monosaccharides, sphingolipids, sterols and other principles to NCS except for fatty acids, glycerides and glycerophospholipids. The higher amounts of TAGs and DAGs in CCS were confirmed by quantitative 1H-NMR, indicating reliable relative quantification of TOF-SIMS. In general, our research developed a novel approach of TOF-SIMS for in situ chemical analysis of TCMs, and its successful application in comparative study of CCS and NCS suggested that TOF-SIMS is an advanced and promising analytical technology for the research of TCMs.
研究领域:
分析化学
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中药学
·
生物化学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 1
·251
·
IF 4.2
Qiulin Li,Yawen Yang,Qingfeng Wang,Xiang Han,Junfeng Zhu,Nan Zhang,Qiuhong Wang,Kanshe Li,Pin Gong,Fuxin Chen
This article applies nuclear magnetic resonance technology to the study of boron-containing traditional Chinese medicine, in order to explore the morphological evolution of boron elements in traditional Chinese medicine. Borax is a traditional Chinese medicine with anti-corrosion, anti-inflammatory, antibacterial, and anticonvulsant effects. It is made by boiling, removing stones, and drying borax minerals like borate salts. This article introduces an11B nuclear magnetic resonance method for identifying and characterizing boron-containing compounds in TCM. We applied this technology to borax aqueous solutions in different chemical environments and found that with boron mixed in the form of SP2hybridization in equilateral triangles and SP3hybridization in equilateral tetrahedra, the pH changes in alkaline environments significantly affected the ratio of the two. At the same time, it was found that in addition to the raw material peak, boron signals of other boron-containing compounds were also detected in 20 commercially available boron-containing TCM preparations. These new boron-containing compounds may be true pharmaceutical active ingredients, and adding them directly to the formula can improve quality and safety. This article describes the detection of11B NMR in boron-containing traditional Chinese medicine preparations. It is simple, non-destructive, and can provide chemical fingerprint studies for boron-containing traditional Chinese medicine.
研究领域:
药学
·
化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Wenguang Wang,Xiaoli Ma,Xiaoyu Guo,Mingbo Zhao,Pengfei Tu,Yong Jiang
In order to solve the bottleneck of reference standards shortage for comprehensive quality control of traditional Chinese medicines (TCMs), a series of strategies, including one single reference standard to determine multi-compounds (SSDMC), quantitative analysis by standardized reference extract (QASRE), and quantitative nuclear magnetic resonance spectroscopy (qNMR) were proposed, and Mahoniae Caulis was selected as an example to develop and validate these methods for simultaneous determination of four alkaloids, columbamine, jatrorrhizine, palmatine, and berberine. Comprehensive comparisons among these methods and with the conventional external standard method (ESM) were carried out. The relative expanded uncertainty of measurement was firstly used to compare their credibility. The results showed that all these three new developed methods can accurately accomplish the quantification by using only one purified reference standard, but each of them has its own advantages and disadvantages as well as the specific application scope, which were also discussed in detail in this paper.
研究领域:
药学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FORENSIC SCIENCES
·
2021
·Vol. 66
·期 5
·2035-2040
·
IF 1.832
Yang Ya,Zhang Zhixiang,Liu Chao,Zhao Wei,Wang Zhiyong,Cheng Huafeng,Zhu Shaohua,Xu Hongfei
Aconitine is a natural toxic substance belonging to the family of aconitum alkaloids, which is used in small doses by Traditional Chinese practitioners. We present a case of accidental death due to acute aconitine poisoning. A 65-year-old Chinese man presented to a medical clinic with fever and shoulder pain. Soon after administration of intravenous ceftriaxone, a bottle of Chinese medicinal wine was administered. The man rapidly experienced headache, hearing loss and tinnitus, nausea, diarrhea, numbness of the lips, tongue, face, and the upper and lower extremities, cold sweat, fall of blood pressure, and discomfort of the entire body, and he died on the same day. Postmortem examination showed cyanosis of the nail beds, severe congestion in lungs and other organs, bilateral pleural effusions, and infiltration of basophils in the intestinal submucosa. No significant increase of IgE was found in the blood. Toxicological analysis detected aconitine in the medicinal wine, blood, and stomach contents by liquid chromatography–tandem mass spectrometry (LC-MS⁄MS). Aconitine was quantified in cardiac blood as 16.4 ng/ml, and in stomach contents (63.8 ng/ml) and wine sample (10.1 ug/ml). The cause of death was aconitine poisoning. This case report highlights the danger of aconitine. LC-MS⁄MS has the advantages of high analytic speed, sensitivity, and accuracy. It would be desirable for the government to control the use of aconitine-containing herbal extracts.
研究领域:
毒理学
·
医学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Essential Oil Bearing Plants
·
2022
·
IF 1.971
Jialun Zhong,Nadeem Muhammad,Xiaoying Yang,Jiasheng Li
Atractylodes chinensis (DC.) Koidz., Magnolia officinalis Rehd. et Wils., Citrus reticulata Blanco, Amomum villosum Lour., Pogostemon cablin (Blanco) Benth., Mosla chinensis Maxim., Zingiber officinale Rosc., and Artemisia argyi Lévl. et Vant are eight of the extensively used herbs among Traditional Chinese Medicines (TCM) with a long history. The essential oil (EO) extracts of the eight TCMs were obtained using supercritical fluid extraction coupled with molecular distillation to investigate the chemical composition, antimicrobial, antioxidant activities, and anti-inflammatory activity for further exploration of the underlying mechanism of the various biological activities. The chemical components of the EO were analyzed by GC-MS. The antimicrobial activity was evaluated by the agar-well diffusion assay and the minimum inhibitory concentration assay. The antioxidant activity of the EO was evaluated by 1, 1-diphenyl-2-picrylhydrazyl radical and 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt assays. The anti-inflammatory activity of a recipe named “Shenshusan” was evaluated by the xylene-induce ear vasodilatation assay and the acetic acid-induce capillary permeability enhancement assay. Chemical constituents of the EO varied markedly among the eight species of TCMs. The EOs from Mosla chinensis and Artemisia argyi exhibited strong antimicrobial activity while the EOs from Magnolia officinalis, Mosla chinensis and Zingiber officinale exhibited strong antioxidant activity. The recipe “Shenshusan” showed significant anti-inflammatory activity (340 mg/kg for mice). This study contributes to what is known about the eight kinds of TCM possesses the potent antimicrobial, antioxidant, and anti-inflammatory activity which held great potential and can help guide future recommendations and interventions for food, medicine, and cosmetics application.
研究领域:
药理学
·
植物化学
·
中医药学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xingyun Zhao,Chengxiao Zhao,Hao Jia,Yifan Niu,Jiangyan Jin,Xiaojian Liu,Zhenyu Li,Xuemei Qin,Ke Li
Hydrothermal carbon (HTC) and modified hydrothermal carbon coated silica microspheres were optimized and applied for the separation of phenolic acids, standard saccharides and natural oligosaccharides in the extract of Traditional Chinese Herbal Medicine (TCM). The materials obtained were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), the BET surface area analysis and the zeta potential. The investigation of the synthesized materials and the purchased amide modified silica stationary phases column was performed with a set of test solutes. Both the synthesized HTC coated silica gel (silica@HTC) and the modified silica@HTC column exhibit excellent hydrophilicity. Notably, the poly(ethyleneimine) (PEI) modified silica@HTC column demonstrates superior chromatographic performance in the separation of saccharides. This work showed significant potential in the modification of novel hydrothermal carbon stationary phases for application in separating polar oligosaccharide compositions from TCM extracts and similar sources.
研究领域:
分析化学
·
中药学
✓ 已验证
产品关联
DOI
高影响因子
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
·
2019
·Vol. 22
·期 2
·113-122
·
IF 1.503
Zhang Xin-guo,Ma Guo-Yan,Kou Fei,Liu Wen-Jie,Sun Qiao-Yun,Guo Guang-Jun,Ma Xiao-Di,Guo Si-Jia,Jian-Ning Zhu
Background: Adenosine deaminase (ADA) is an important enzyme in purine metabolism and is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Traditional Chinese Herbal Medicine (TCHM) is widely used alone or in combination with chemotherapy
to treat cancer, due to its ability to deliver a broad variety of bioactive secondary metabolites as promising sources of novel organic natural agents. Objective: In the present study, 29 varieties of medicinal plants were screened for the presence of ADA inhibitors. Results: Extracts
from Reynoutria japonica, Glycyrrhiza uralensis, Lithospermum erythrorhizon, Magnolia officinalis, Gardenia jasminoides, Stephania tetrandra, Commiphora myrrha, Raphanus sativus and Corydalis yanhusuo demonstrated strong ADA inhibition with rates greater than 50%. However, Reynoutria japonica
possessed the highest ADA inhibitory activity at 95.26% and so was used in our study for isolating the ADA inhibitor to be further studied. Eight compounds were obtained and their structures were identified. The compound H1 had strong ADA inhibitory activity and was deduced to be emodin by
1H and 13C-NMR spectroscopic analysis with an IC50 of 0.629 mM. The molecular docking data showed that emodin could bind tightly to the active site of ADA. Our results demonstrated that emodin displayed a new biological activity which is ADA inhibitory activity with high cytotoxic activity
against K562 leukemia cells. The bioactivity of cordycepin was significantly increased when used in combination with emodin. Conclusion: Emodin may represent a good candidate anti-cancer therapy and adenosine protective agent.
研究领域:
药理学
·
肿瘤学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）