Kai Wu,Junhao Li,Hanzhang Ran,Chen Zhao,Jiaze Gao,Yuan Tian,Chengheng Wu,Jie Ding,Dan Wei,Yunlong Yu,Yanfei Tan,Jing Sun,Hongsong Fan
Hemostatic materials combining rapid bleeding control with regenerative potential remain a major clinical need. Here, we report a bioinspired hemostatic powder (GSHD) engineered through molecular conjugation of thiolated gelatin and dopamine-modified hyaluronic acid. Upon contact with biofluids, GSHD rapidly gels via dynamic thiol-catechol cross-linking, achieving ultrafast hydration (<5 s) and forming a robust hydrogel. This hydrogel exhibits high blood absorption (569.2 ± 46.3%), strong tissue adhesion (28.3 ± 3 kPa), and burst pressure resistance (216.2 ± 10.5 mmHg), enabling effective sealing under high-flow conditions. In vivo tests in rat liver, cardiac, and femoral artery models show rapid hemostasis (<30 s) with minimal blood loss. Additionally, GSHD promotes M2 macrophage polarization, enhances angiogenesis, and supports organized collagen deposition, facilitating tissue regeneration. By integrating rapid sealing, antioxidant activity, and biomimetic extracellular matrix cues, GSHD represents a paradigm shift in hemostatic design, bridging emergency care with functional tissue repair.
Manshan Xie,Yunhua Chen,Qian Yang,Qingtao Li,Runlin Zhang,Wenzhi Bi,Yong-Guang Jia,Paul K. Chu,Huaiyu Wang,Xuetao Shi
Bioadhesives or sealants have received considerable attention for suture-less wound sealing. However, most adhesive biomaterials involve complex molecular designs which lack dynamic mechanical properties , inherent hemostasis , as well as the capability of preventing postsurgical tissue adhesion. Herein, a nano-enabled supramolecular hydrogel sealant based on complementary DNA duplexes combined with multiple physicochemical crosslinks is designed and demonstrated. This supramolecular sealant exhibits excellent dynamic reversibility , low swelling, and wet adhesive properties . It can achieve rapid sealing of damaged tissues, blood coagulation , as well as hemostasis. Furthermore, DNA molecules endow the sealant with promising capabilities of preventing protein absorption, cell adhesion and postsurgical tissue adhesion. The in vivo evaluation based on the gastric perforation repair model shows that the sealant can improve granulation tissue growth, collagen deposition, and vascularization and facilitate gastric perforation closure. The nano-enabled DNA supramolecular hydrogel sealant represents a promising alternative for gastric perforation repair and has large clinical potential pertaining to suture-less repair of soft tissues. Data availability The data reported in this manuscript are available upon request.
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