To develop biocompatible nanofibrous membranes for wound healing, we investigated the coelectrospinning of two proteins (collagen and zein) in aqueous acetic acid solution. It was found that the combination of zein could improve the electrospinnability of collagen. For the resultant electrospun membrane, its fiber diameter, surface wettability, mechanical, and in vitro degradable properties as well as cell adhesive ability could be modulated by the change of collagen/zein blending ratio. Moreover, berberine drug could be incorporated in situ into the electrospun nanofibrous membrane for its controlled release and antibacterial activity. The addition of berberine showed little effects on the fiber morphology and cell viability. In addition, the wound healing performance of the as-obtained nanofibrous membranes was examined in vivo by using female Sprague–Dawley rats and histological observation.
Li Fangling,Han Xiaoman,Cao Dongdong,Yin Junxia,Chen Li,Li Dongmei,Cui Lin,Liu Zhiyong,Guo Xuhong
Hemostatic dressings with multiple functions are superior to current hemostatic dressings for use in the complex situation of emergency accidents. In particular, the existing dressings lack consideration for the prevention of hypothermic shock after massive hemorrhage. In this study, gelatin (GN) and oxidized pectin (OP) were used for Schiff base cross-linking, and then polyvinyl alcohol (PVA) solution mixed with hemostatic caffeic acid (CA) was introduced to obtain aerogel substrate material (CB) after lyophilization. Polydimethylsiloxane (PDMS) and silver nanowires (Ag NWs) were used to construct a hydrophobic layer, an antibacterial layer and an infrared reflective layer on both sides of CB to prepare a multifunctional aerogel wound dressing with heat preservation, antifouling, hemostasis and antibacterial properties (PDMS-Ag NW-CB). The results showed that the infrared transmittance of PDMS-Ag NW-CB is almost 0, so that thermal energy loss from the body is minimized. The contact angles with water and blood are 129° and 120°, respectively, which have the effect of antifouling. This dressing can absorb blood quickly within 10 min, adhere to and gather platelets, and achieve hemostasis. It has good antibacterial and biocompatibility. Therefore, PDMS-Ag NW-CB has great potential in application to emergency treatment.
危险品化学品经营许可证（带存储）