Analytical Methods
·
2024
·
IF 2.7
Wenjuan Zhang,Mingwan Luo,Juan Chen,Zhengxing Li,Xian Wei,Miqi Wu,Shengli Yang,Yuanju He,Xiaoping Wang,Zhiyou Xiao
The intrinsic fluorescence of berberine is very weak, which can be enhanced by its interaction with specific aptamers. A simple and sensitive DNA sensor for visual detection of berberine has here been established. When using this sensor, there was a good linear relationship between the change in fluorescence intensity of berberine and the concentration of berberine in the range of 16–2000 nM, with a detection limit of 5.1 nM. The change in fluorescence intensity was caused by the addition of aptamers. A detection limit of 170.1 nM was acquired by reading the RGB values of fluorescent images with a smartphone for the quantification of berberine. Common antibiotics did not interfere with the measurement of the berberine concentration. The molecular ion peaks of the complexes formed by the aptamer and berberine could be clearly observed by electrospray ionization mass spectrometry. The UV-vis absorption spectra, circular dichroism spectra, and fluorescence spectra indicated a strong interaction between berberine and the aptamer. The dissociation constant (Kd) between berberine and the aptamer was 1.91 μM. This sensor was both simple and sensitive, requiring only a 21-base oligonucleotide. It realized a visual quantitative analysis with a smartphone. This method could also be used for similar fluorescence visualization determination of aptamer-based drug molecules.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
NORDIC PULP & PAPER RESEARCH JOURNAL
·
2023
·Vol. 38
·期 2
·271-284
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IF 1.2
Gui Fu,Zhenzhen Chen,Yingping Qi,Hua Li
In order to solve the problem of bacterial resistance, increase the antibacterial performance and delay the aging of paper, the berberine modified trimethoprim carboxymethyl cellulose was prepared using trimethoprimethylate carboxymethyl cellulose as raw material and trimethoprimethylate lactate (TMP) as antibacterial modifier by Schiff Base reaction. At the same time, berberine (BBR) was used as graft monomer to prepare a multifunctional protective solution integrating strengthening, antibacterial and good biocompatibility. The optimal process conditions were determined by single factor experiment and response surface method. The structure and properties of the product were characterized by FT-IR, XRD, TG and SEM and the antibacterial properties were evaluated by AGAR disk diffusion method. The results showed that when the mass ratio of dialdehyde carboxymethyl cellulose and modified berberine was 7:15, reaction temperature was 40 °C, and pH was 6.0, the optimum synthetic conditions was obtained. And the minimum inhibitory concentration of the product against Staphylococcus aureus and Escherichia coli were 0.2 mg/mL, minimum diameter of antibacterial zone of 6.7 mm and 7.1 mm, respectively, with good antibacterial effect. This paper lays a foundation for further research on the safe and effective antiquities antibacterial agent, and provides a reference for its further development in paper protection.
研究领域:
材料科学
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微生物学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 44
·25201-25209
·
IF 2.936
Li-rong Nie,Hang Song,Alula Yohannes,Siwei Liang,Shun Yao
A magnetic ionic liquids (MILs)-based aqueous two-phase system (MIL-ATPs) obtained by mixing with a series of inorganic salts, which involves five cholinium MILs with the piperidinyloxy radical anion is reported for the first time. Phase diagrams for the new ATPs were experimentally determined at different temperatures (298.15–318.15 K) and the liquid–liquid equilibrium data for two-phase systems were correlated according to the empirical nonlinear expression. The effects of the types of MILs, temperature and inorganic salts on the binodal curve are discussed in detail. The MIL-ATPs coupled with HPLC-UV analysis was developed in the quantitation of berberine hydrochloride in Rhizoma coptidis. Under optimal conditions, the partition coefficient of berberine hydrochloride was 127.68 with the precision values (RSD%) of 1.40% and 2.83% for intra-day (n = 6) and inter-day (n = 3), respectively. The limit of detection (LOD) and limit of quantification (LOQ) for berberine hydrochloride were 0.023 mg L−1 and 0.077 mg L−1, respectively. The recoveries were obtained in the acceptable range of 97.4–101.2%. Moreover, the content of berberine hydrochloride in the raw material of Rhizoma coptidis was measured as 123.95 mg g−1 with this method. Finally, 99.8% MIL was recovered for cycle application after the removal of berberine hydrochloride by using D101 resin. This study provides a meaningful reference for the application of MIL-ATPs with great prospects.
研究领域:
分析化学
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中药学
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产品关联
DOI
PubMed
高影响因子
Biofabrication
·
2025
·Vol. 17
·期 3
·035012
·
IF 8
Mengyuan Li,Jiaming Ge,Jingwen Yao,Yuanhao Zhang,Lin Ma,Zheng Li,Xiangli Han,Ming Liu,Fei Tian,Jing Zhao
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck. Due to low bioavailability and passive targetability of anticancer drugs show great limitations in cancer therapy, the treatment of OSCC faces major challenges. Folic acid (FA) targeting can deliver anticancer drugs efficiently into the tumor environment, further enhance the anti-cancer efficacy. Herein, the nanoplatform based on UiO-66 that encapsulated with an effective FA targeting ligands and the pH-responsive polyethylene glycol (PEG) layer for the targeted delivery of berberine (Ber) is constructed for fighting against OSCC. The FA modification and controlled pH-responsiveness enable the targeted delivery of UiO-66/PEG-FA, which promotes the release of Ber and increases the cumulative intracellular Ber concentration, which both promote consumption of glutathione (GSH) and induced generation of reactive oxygen species (ROS), further stimulate the secretion of inflammatory factors (TNF-α and IL-1β). A comprehensive evaluation of in vitro and in vivo experiments show that UiO-66@Ber/PEG-FA promote autophagy and apoptosis of tumor cells by regulating the expression of Beclin-1, ATG13, BAX and Bcl-2, and effectively inhibit tumor growth. Overall, UiO-66@Ber/PEG-FA exhibit superior pH-responsiveness and targeted therapeutic efficiencies in vitro and vivo, it can serve as an approach for OSCC therapy.
研究领域:
肿瘤学
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药剂学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Yizhen Zhang,Lifen Liu,Qiyuan Chen,Yun He,Michael K.H. Leung
A novel system including peroxymonosulfate (PMS) activation process in a photocatalytic fuel cell (PFC) was established, promoting the degradation efficiency of berberine and enhancing the simultaneous electricity generation. The cathode is based on carbon-fiber cloth (CC) and loaded CoFe 2 O 4 with carbon nano-fibers (CNFs), matching well with Ag NPs/ZnO NRs/NiF photo-anode to drive PFC work. The CNFs/CoFe 2 O 4 /CC cathode is multifunctional, it can activate PMS for sulfate radical ( SO 4 - · ) generation, and can simultaneously accept electrons from photo-anode for transform between Co 3+ and Co 2+ . The adding of PMS in PFC system boosts radical formation and cyclic reactions between related species. This PFC-PMS system showed a high antibiotic degradation efficiency and stable electricity generation (93.5% for berberine degradation, 9.18 h −1 for kinetic rate constant, ~0.50 V for cell-voltage at 2000 Ω). In addition, the pollutant removal capacity, influencing factors and the pollutant degradation mechanism in PFC-PMS system were illustrated. The feasible mechanism of berberine degradation was elucidated by the radical trapping and EPR test and these results confirmed that the formation of reactive species, including SO 4 - · , OH, O 2 - · , 1 O 2 radicals and holes. This study suggests that the established PFC-PMS system is more efficient in wastewater treatment and simultaneous energy recovery.
研究领域:
环境科学
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能源技术
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化学工程
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DOI
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 42
·e202203178
·
IF 2.307
Yuanxiang Fu,Hengdan Si,Juan Chen,Wenjuan Zhang,Shuang Feng,Zhiyou Xiao
Graphical Triplex DNA plays an essential role in molecular recognition, gene regulation, diagnosis, and treatment of genetic diseases. It is crucial to screen intercalators to enhance triplex DNA stability. We established a novel “turn-on” fluorescence sensor for screening triplex DNA binders based upon molecular beacon and target dsDNA. The working principle of DNA fluorescence sensor was confirmed by circular dichroism experiments and melting curve tests. Triplex DNA plays an essential role in molecular recognition, gene regulation, diagnosis, and treatment of genetic diseases. However, triplex DNA has some shortcomings, such as poor internal Hoogsteen hydrogen bond stability and the difficult formation of parallel triplex DNA under physiological conditions, limiting its applications to a certain extent. Therefore, it is crucial to screen intercalators to enhance triplex DNA stability. Herein, we established a novel “turn-on” fluorescence sensor for screening triplex DNA binders based upon molecular beacon. Eight different organic small molecules were screened. The fluorescence of the sensor was significantly enhanced in the presence of berberine, coralyne, palmatine, and neomycin. These four small molecules affect stabilizing triplex DNA as an intercalator; the other four small molecules, kanamycin, tetracycline, ethidium bromide, and 4 ′, 6-diamidino-2-phenylindole did not show any noticeable intercalation effect. The results of circular dichroism and melting curve were consistent with those of the fluorescence spectrum.
研究领域:
生物化学
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分子生物学
·
遗传学
✓ 已验证
DOI
高影响因子
COMPUTERS IN BIOLOGY AND MEDICINE
·
2024
·
IF 7
Chengxiang Hu,Fuyan Cao,Yongxin Jiang,Kaifeng Liu,Tao Li,Yin Gao,Wannan Li,Weiwei Han
Chronic atrophic gastritis (CAG), characterized by inflammation and erosion of the gastric lining, is a prevalent digestive disorder and considered a precursor to gastric cancer (GC). Coptis chinensis France (CCF) is renowned for its potent heat-clearing, detoxification , and anti-inflammatory properties. Zuojin Pill (ZJP), a classic Chinese medicine primarily composed of CCF, has demonstrated effectiveness in CAG treatment. This study aims to elucidate the potential mechanism of CCF treatment for CAG through a multifaceted approach encompassing network pharmacology , molecular docking, molecular dynamics simulation and experimental verification. The study identified three major active compounds of CCF and elucidated key pathways, such as TNF signaling, PI3K-Akt signaling and p53 signaling. Molecular docking revealed interactions between these active compounds and pivotal targets like PTGS2, TNF, MTOR, and TP53. Additionally, molecular dynamics simulation validated berberine as the primary active compound of CCF, which was further confirmed through experimental verification. This study not only identified berberine as the primary active compound of CCF but also provided valuable insights into the molecular mechanisms underlying CCF’s efficacy in treating CAG. Furthermore, it offers a reference for refining therapeutic strategies for CAG management.
研究领域:
药理学
·
分子生物学
·
中医药学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yundi Wu,Xudong Liu,Can Yao,Jianqiang Chen,Xilong Wu,Mingqiang Zhu
Autophagy is a process that eliminates damaged cells and malfunctioning organelles via lysosomes, which is closely linked to cancer. Primaquine (PQ) was reported to impede autophagy flow by preventing autophagosomes from fusing with lysosomes at the late stage of autophagy. It will lead to cellular metabolic collapse and programmed cell death. Excessive or extended autophagy enhances the efficacy of chemotherapeutic drugs in cancer prevention. The utilization of autophagy inhibition in conjunction with chemotherapy has become a prevalent and reliable approach for the safe and efficient treatment of cancer. In this work, an acid-sensitive nanoprodrug (O@PD) targeting CD44 receptors was produced using Schiff-base linkages or electrostatic interactions from oxidized hyaluronic acid (OHA), PQ, and doxorubicin (DOX). The CD44-targeting prodrug system in triple-negative breast cancer (TNBC) cells was designed to selectively release DOX and PQ into the acidic tumor microenvironment and cellular endosomes. DOX was employed to investigate the cellular uptake and ex-vivo drug distribution of O@PD nanoprodrugs. PQ-induced autophagy suppression combined with DOX has a synergistic fatal impact in TNBC. O@PD nanoprodrugs demonstrated robust anticancer efficacy as well as excellent biological safety, making them suitable for clinical use.
研究领域:
癌症研究
·
药学
·
纳米技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）