TALANTA
·
2018
·
IF 4.244
Dandan Jiang,Xiqian Li,Xueju Lv,Qiong Jia
Dendrimers exhibit tunable terminal functionality and bio-friendly nature, making them of being promising materials for applications in the field of separation and enrichment. In this work, we prepared magnetic hydrazide-functionalized poly-amidoamine (PAMAM) dendrimer embedded with TiO 2 for the enrichment of phosphopeptides. The novel affinity probe possessed superparamagnetism , realizing its rapid separation from sample solution. Electrostatic attraction and hydrogen bonding existed between PAMAM and phosphopeptides while Lewis acid-base interaction was originated between TiO 2 and the targets. The combined synergistic strength of multiple binding interactions contributed to the highly selective enrichment of phosphopeptides. The specificity for the capture of phosphopeptides was reflected in quantities as low as 1:1000 mass ratio of phosphopeptides to non-phosphopeptides. The detection limit of β -casein digests was low to 0.4 fmol, indicating the high sensitivity of the developed method. Fifteen and four phosphopeptides could be selectively captured from non-fat milk digests and human serum samples, which further confirmed the great potential of the affinity probe in the extraction of low-abundance phosphopeptides from real complex biological samples.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 25
·4402-4409
·
IF 4.872
Nan Lv,Zhigang Wang,Wenzhi Bi,Guangming Li,Jilin Zhang,Jiazuan Ni
The novel chloro(dimethyl)octylsilane (C8)-functionalized [CeO2//SiO2] Janus fibers (denoted CeO2//SiO2-C8) have been successfully prepared by electrospinning technique and subsequent modification. The CeO2//SiO2-C8 Janus fiber composed of the side-by-side CeO2 fiber and SiO2 fiber modified by C8 is endowed with separate affinity sites and hydrophobic chains. It has a relatively high specific surface area of 127.4 m2 g−1 with an average pore size of 4.1 nm. The potential of CeO2//SiO2-C8 Janus fibers for the selective capture and individual MS detection of low-abundance endogenous peptides and phosphopeptides from the tryptic protein digest and human serum real sample was explored and evaluated for the first time. The experimental results demonstrate that the CeO2//SiO2-C8 Janus fiber can selectively extract phosphopeptides in virtue of high selective affinity of CeO2 to phosphate groups and enrich low-abundance peptides on account of the strong hydrophobic interaction of C8 chains. Moreover, the enrichment performance of the CeO2//SiO2-C8 probe is superior to that of commercial products (e.g. TiO2 nanoparticles and ZipTipC18 pipette tips) available. Overall, the efficient enrichment capability of CeO2//SiO2-C8 Janus fibers makes them a prospective candidate for the capture of low-abundance endogenous peptides and phosphopeptides from complex biological samples for individual MS detection.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Hongmei Yang,Linxu Wang,Zhaoyang Xie,Simeng Shao,Yi Wu,Weiyin Xu,Bin Gu,Bo Wang
Comprehensive sulfur-nitrosylation (SNO) proteome coverage in complex biological systems remains challenging as a result of the low level of endogenous S-nitrosylation and its chemical instability. Herein, we optimized the synthesis route of SNOTRAP (SNO trapping by triaryl phosphine) probe and the proteomics pipeline (including preventing over-alkylation, sample washing, trypsin digestion). Preventing overalkylation was found to be the key factor resulting in a higher number of identified SNO proteins by evaluating various experimental conditions. With the improved SNOTRAP-based proteomic pipeline, we achieved an improvement of ∼10-fold on identification efficiency, and identified 1181 SNO proteins (1714 SNO sites) in mouse brain, representing the largest repository of endogenous S-nitrosylation. Moreover, we identified the consensus motif of SNO sites, suggesting the correlation with local hydrophobicity, acid-base catalysis, and the surrounding secondary structures for modification of specific cysteines by NO. Collectively, we provide a universal pipeline for the high-coverage identification of low-abundance SNO proteins with high enrichment efficiency, high specificity (98%), good reproducibility, and easy implementation, contributing to the elucidation of the mechanism(s) of nitrosative stress in multiple diseases.
研究领域:
生物化学
·
蛋白质组学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 22
·10936-10944
·
IF 6.233
Hao Wan,Hongqiang Qin,Zhichao Xiong,Weibing Zhang,Hanfa Zou
Magnetic mesoporous carbon microspheres with a yolk–shell structure (YSMMCS) have been prepared via a new in situ carbon source strategy. The material was fabricated by two shells coated onto the Fe3O4 particles; the inner dense and thick silica shell could protect the magnetic core from harsh acidic solvents as well as induce the void between the core and the outer shell for the yolk–shell structure, while the outer organosilica shell was used as the template and carbon source for in situ preparation of a carbon shell with mesoporous structure. A C18-alkyl chain was incorporated in situ as the carbon precursor efficiently, avoiding the conventional infiltration step, which was very difficult to manipulate and time-consuming with the possibility of losing the carbon precursor. The resulting yolk–shell magnetic mesoporous carbon microspheres exhibited a high surface area (273.15 m2 g−1), a large pore volume (0.31 cm3 g−1), and a strong magnetic response (a saturation magnetization value of 34.57 emu g−1). As a result of the void between the core and the outer shell and the π–π stacking effect, adsorption capacity reached 191.64 mg g−1 by using Rhodamine B as a standard analyte, indicating the great potential application of the material as drug carriers. Owing to the inherent hydrophobicity and high surface area, the composite material showed better performance in the enrichment of peptides than a magnetic mesoporous silica material (Fe2O3@nSiO2@mSiO2). According to the LC-MS/MS results, about 51 and 29 nonredundant peptides were identified from tryptic digests of 5 nM BSA. Additionally, taking advantage of the mesoporous structure and strong magnetic response, the material was utilized to selectively extract low abundance endogenous peptides from human serum in the presence of high abundance proteins. Based on the LC-MS/MS results, 962 endogenous peptides were obtained by 2.5 mg YSMMCS relative to 539 endogenous peptides by 5 mg Fe2O3@nSiO2@mSiO2, confirming the outstanding performance of YSMMCS in peptidome analysis.
研究领域:
材料科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Jia-yuan Li,Xing-yu Long,Dong Sheng,Hong-zhen Lian
The iron oxide nanoparticles (Fe 3 O 4 ) were prepared by organic molecule-assisted method in aqueous solution. The facile synthetic process of Fe 3 O 4 nanoparticles was conducted only by mixing FeCl 2 and 2-methylimidazole (2-MIM) without any additives. A possible growth mechanism of the Fe 3 O 4 nanocrystals was proposed for this mild reaction. Then, the Fe 3 O 4 nanoparticles were anchored onto graphene oxide (GO) sheets in water by ultrasound-assisted method, forming an affinity probe with strong biocompatibility. Due to the hydroxy and carboxylic groups of GO sheets, Fe 3 O 4 /GO probe exhibits excellent performance for enriching low-abundance hydrophilic peptides, while the Fe 3 O 4 nanoparticles endure the probe with specific affinity to phosphopeptides. The analytical protocol was developed for sequential enrichment of low-abundance peptides and phosphopeptides by the affinity probe. It exhibited the sequence coverage of 26% for capture of 17 low-abundance peptides from bovine serum albumin (BSA), as well as the selectivity of 1:1:100 for phosphopeptides from α-/β-casein/BSA, and low detectable concentration of 2.5 fmol and probe reusability of 5 times for capture of phosphopeptides from α-/β-casein. Consequently, the prepared Fe 3 O 4 /GO material possesses excellent feature as multifunctional affinity probe for low-abundance peptides including phosphopeptides from complex biological matrices detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2014
·
IF 4.287
Yingshuai Liu,Yuanyuan Zhang,Yanan Zhao,Jie Yu
There still has a big challenge for low-abundance protein detection with antibody microarrays. In this work, phenylboronic acid (PBA) polymer brush-enabled oriented, high density, and covalent antibody immobilization was realized for sensitive antibody microarrays. PBA-enabled oriented antibody attachment via carbohydrate at Fc portion keeps antigen-binding sites fully expose to their corresponding antigens, resulting in higher antibody–antigen (Ab–Ag) binding efficiency. Sandwich immunoassay with rabbit IgG as model analyte was performed on poly(glycidyl methacrylate)-amino-phenylboronic acid-coated glass slide (PGMA-APBA-slide). One order improvement of LOD was achieved as compared with that on poly(glycidyl methacrylate) glass slide (PGMA-slide). The improvement is mainly attributed to PBA-assisted high density and oriented antibody immobilization. This work provides a versatile and effective strategy to develop high sensitive antibody microarrays for low-abundance protein analysis in various proteomic applications.
研究领域:
生物化学
·
免疫学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 30
·3661-3669
·
IF 0
Gong Cheng,Yan-Lin Liu,Zhi-Gang Wang,Shui-Ming Li,Ji-Lin Zhang,Jia-Zuan Ni
Architectural design is important to achieve appropriate chemical and biological properties of nanostructures. Yolk–shell magnetic microspheres consisting of Fe3O4 cores and porous and hollow YPO4 affinity shells have been designed and constructed. The yolk–shell magnetic microspheres have a saturation magnetization (Ms) value of 48.3 emu g−1 with a negligible coercivity and remanence at 300 K and a BET specific surface area of 12.5 m2 g−1 with an average pore size of 10.1 nm for the mesoporous YPO4 shell. The novel multifunctional yolk–shell nanostructures can realize the selective capture, convenient magnetic separation and rapid identification of target phosphopeptides by taking advantage of the Fe3O4 magnetic cores and the selective YPO4 affinity shells. Sensitivity and selectivity of the affinity yolk–shell nanostructures were evaluated by digests of standard proteins and complex biosamples. 2678 unique phosphopeptides were captured and identified from the digest of mouse brain proteins. Therefore, this work will be highly beneficial for future applications in the isolation and purification of biomolecules, in particular, low-abundance phosphopeptide biomarkers.
研究领域:
生物化学
·
纳米医学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Ji Ji,Lei Nie,Yixin Li,Pengyuan Yang,Baohong Liu
A facile online enrichment protocol has been proposed based on microfluidic droplets acting as an interface between a liquid chromatography separation system and detection systems of ESI-MS/MS and laser-induced fluorescence. Low-abundance species were successfully concentrated and analyzed in this system via droplet shrinkage. The proposed platform significantly increased the enrichment efficiency and detection sensitivity with reduced sample handling steps, short analysis time, and no cross-contamination. The presented system is universal, shows no discrimination, and is easily coupled with other separation and detection approaches.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Man Zhao,Yiqin Xie,Hemei Chen,Chunhui Deng
Zeolitic imidazolate frameworks (ZIFs) are composed of tetrahedral transition metal ions and imidazolate type organic linkers. This kind of metal organic frameworks (MOFs) combines the unique properties of both zeolites and MOFs. Furthermore, ZIFs can be assembled under mild biocompatible conditions. These advantages make them be primarily studied for biochemical analysis. In this work, polydopamine-modified hydrophilic magnetic ZIFs were synthesized in a simple and mild way for the efficient extraction of low-abundance peptides. Thanks to the low-coordinated Zn 2+ ions, the Fe 3 O 4 @PDA@ZIF-8 composites showed strong affinity towards low-abundance peptides, especially those with histidine residues . After enrichment, the sequence coverage of BSA and HSA tryptic digest got a significant increase. The solid phase method based on the magnetic ZIFs exhibited size-exclusion effect towards large-sized proteins, and proved to be effective even under the 200-fold dilution with BSA protein solution.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2017
·Vol. 28
·期 1
·1704458
·
IF 12.124
Yueshuang Xu,Huan Wang,Chengxin Luan,Fanfan Fu,Baoan Chen,Hong Liu,Yuanjin Zhao
The development of a highly sensitive platform for multiplex circulating microRNAs (miRNAs) detection is important for clinical diagnosis. Here, a new type of porous hydrogel encapsulated photonic crystal (PhC) barcodes is presented with integrated rolling circle amplification (RCA) strategy for multiplex miRNA quantification. As the surrounding porous hydrogel shells of the PhC barcodes are interconnected inverse opal structure with hydrophilic scaffolds, they can provide homogeneous water surrounding for the miRNA targets reaction and RCA. The encapsulated PhC cores of the barcodes can offer stable diffraction peaks for encoding different miRNAs and their RCAs during the detection. By integrating the advantages of PhC barcodes and RCA, it is demonstrated that the technology shows acceptable accuracy and detection reproducibility for the rapid quantification of low-abundance miRNAs, with the limits of detection of 20 fM. Thus, the proposed porous hydrogel encapsulated PhC barcodes provide a new platform for the multiplex quantification of low-abundance targets for practical applications.
研究领域:
生物医学工程
·
分子诊断学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Xiao-Na Wei,Hui-Long Wang
Highly selective enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel affinity material has been synthesized to improve the enrichment specificity for phosphopeptides by using PAAS as coupling molecule. In the resulting materials, highly abundant titanium is available for selective enrichment of phosphopeptides, with plenty of carboxylate groups that can inhibit nonspecific adsorption. The enrichment results demonstrated that the hydrophilic PAA-Ti/TiO 2 composite possesses excellent selectivity for phosphopeptides even at a very low molar ratio of phosphopeptides/non-phosphopeptides (1:1000), extreme sensitivity (the detection limit was at the fmol level), and high recovery of phosphopeptides (as high as 78%). Moreover, the as-prepared nanocomposite provides effective enrichment of phosphopeptides from real samples (mouse liver), showing great potential in the detection of low-abundance phosphopeptides in biological samples.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2013
·
IF 5.093
Dejing Liu,Qian Yang,Susu Jin,Yingying Song,Junfei Gao,Ying Wang,Huaifeng Mi
Core–shell molecular imprinting of nanomaterials overcomes difficulties with template transfer and achieves higher binding capacities for macromolecular imprinting, which are more important to the imprinting of natural low-abundance proteins from cell extracts. In the present study, a novel strategy of preparing core–shell nanostructured molecularly imprinted polymers (MIPs) was developed that combined the core–shell approach with assistant recognition polymer chains (ARPCs). Vinyl-modified silica nanoparticles were used as support and ARPCs were used as additional functional monomers . Immunoglobulin heavy chain binding protein (BiP) from the endoplasmic reticulum (ER) was chosen as the model protein. The cloned template protein BiP was selectively assembled with ARPCs from their library, which contained numerous limited-length polymer chains with randomly distributed recognition and immobilization sites. The resulting complex was copolymerized onto the surface of vinyl-modified silica nanoparticles under low concentrations of the monomers. After template removal, core–shell-structured nanoparticles with a thin imprinted polymer layer were produced. The particles demonstrated considerably high adsorption capacity, fast adsorption kinetics and selective binding affinities toward the template BiP. Furthermore, the synthesized MIP nanoparticles successfully isolated cloned protein BiP from protein mixtures and highly enriched BiP from an ER extract containing thousands of kinds of proteins. The enrichment reached 115-fold and the binding capacity was 5.4 μg g −1 , which were higher than those achieved by using traditional MIP microspheres . The advantageous properties of MIP nanoparticles hold promise for further practical applications in biology, such as protein analysis and purification.
研究领域:
生物化学
·
纳米技术
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）