JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Zhang Cheng-lu,Liu Chang,Nie Shi-ru,Zhang Yang,Guo Jing-hao,Li Xiang-ling,Liu Cui
For the efficient detection of Hg 2+ and ClO − , a double-analyte-responsive fluorescent probe PTB was successfully synthesized by combining N-butyl-3-formyl phenothiazine with hydrazine benzothiazole, and designing a specific reaction site for recognizing two analytes (Hg 2+ and ClO − ) in a compound. It was shown that probe PTB successfully formed a stable complex with Hg 2+ in the coordination ratio of 2:1 by using the strong sulfur affinity of Hg 2+ , which resulted in a remarkable "turn-off" effect, with a quenching efficiency of 92.5% and four reversible cycles of Hg 2+ fluorescence detection. For the fluorescence detection of Hg 2+ , the response time is fast (≤ 2 min) and the detection limit is low (7.8 nM), showing extremely high sensitivity, and the performance is obviously better than that of the reported fluorescent probes for detecting Hg 2+ . In particular, probe PTB has low toxicity and good biocompatibility, and has been successfully used for imaging of Hg 2+ in living cells. Moreover, probe PTB uses thioether bond and carbon-nitrogen double bond as reaction sites to detect ClO − , which has large Stokes Shift (149 nm), good selectivity, high quenching efficiency (96.5%) and fast time response (about 10 s), and successfully detects ClO − in actual water samples. Graphical The dual functional fluorescent probe PTB is sensitive for Hg 2+ and ClO − . It has been successfully used for making pH fluorescent test paper and imaging detection of exogenous Hg 2+ in VSMC cells with low toxicity.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
ACS Applied Electronic Materials
·
2025
·
IF 4.7
Runqiu Wu,Bendong Liu,Hongye Qin,Dongkun Yu,Jiahui Yang,Haibin Liu,Guohua Gao
The development of flexible wearable devices requires energy supply devices with high flexibility and stretchability. Thermoelectric ionic hydrogels possess characteristics of high flexibility and stretchability and can be used as heat conversion devices to transform low-grade heat energy into electrical energy. Recently, studies of ionic-thermoelectric hydrogels exhibit high thermopower (or Seebeck coefficient), but their raw materials are typically laced with strong alkalis or heavy metals, which makes them highly toxic and limits their potential applications. In order to enhance the thermopower in this study, we employed PEDOT:PSS as an n-type dopant in PAAm hydrogels to induce counterion condensation. This process immobilizes cations, thereby amplifying the ion-thermal migration rate disparity in the Soret effect and enhancing the thermopower. Furthermore, the thermoelectric performance was optimized by adjusting the dosage of the initiator ammonium persulfate (APS). The experimental results showed that the PEDOT:PSS/PAAm-based ionic hydrogel exhibited a high thermopower of −4.45 mV/K when the concentration of APS was 1 wt %. This study provides an approach for the preparation of low-toxicity, high-thermopower n-type thermoelectric ionic hydrogels.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMBIOCHEM
·
2015
·Vol. 16
·期 11
·1622-1628
·
IF 3.088
Rongqiang Liao,Shouhui Yi,Manshuo Liu,Wenling Jin,Bo Yang
Graphical Get smart: A smart targeting gene carrier for cancer-specific delivery has been successfully prepared by a “multilayer bricks-and-mortar” strategy. Carriers produced by two different self-assembly schemes were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the most suitable (Method A) material has lower cytotoxicity than 25 kDa bPEI and that transfection efficiency is maintained. A targeting gene carrier for cancer-specific delivery was successfully developed through a “multilayer bricks-mortar” strategy. The gene carrier was composed of adamantane-functionalized folic acid (FA-AD), an adamantane-functionalized poly(ethylene glycol) derivative (PEG-AD), and β-cyclodextrin-grafted low-molecular-weight branched polyethylenimine (PEI-CD). Carriers produced by two different self-assembly schemes, involving either precomplexation of the PEI-CD with the FA-AD and PEG-AD before pDNA condensation (Method A) or pDNA condensation with the PEI-CD prior to addition of the FA-AD and PEG-AD to engage host–guest complexation (Method B) were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the material produced by the Method A assembly scheme has lower cytotoxicity than branched PEI 25 kDa (PEI-25KD) and that the transfection efficiency is maintained. These findings suggest that the gene carrier, based on multivalent host–guest interactions, could be an effective, targeted, and low-toxicity carrier for delivering nucleic acid to target cells.
研究领域:
生物医学工程
·
分子生物学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 29
·22343-22349
·
IF 3.84
Zhao Chen,Xiaochi Wang,Hua Li,Chao Li,Qinghua Lu,Guang Yang,Jiangang Long,Lingjie Meng
A facile but effective bottom-up method for the mass preparation of N-doped carbon dots (N–C-dots) was developed by the direct heating of a solid mixture of folic acid (FA) and sodium citrate (SC) for several minutes at 300 °C. The nitrogen content of the resulting N–C-dots could be easily and precisely tuned from 0% to 14% by adjusting the SC to FA mass ratio. Uniform N–C-dots with honeycomb-like ordered structures were obtained, with sizes ranging from 1 to 3 nm in diameter. The N-doping content affected not only the emission quantum yield but also the emission wavelength. The N–C-dots emitted strong blue-green fluorescence based on the excitation wavelength and N-doping content. Because of their excellent water solubility, low toxicity, powerful fluorescence, and high resistance to photobleaching, the N–C-dots can enter various cells and serve as ideal candidates for multicolour cell imaging.
研究领域:
材料科学
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生物医学工程
·
化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2019
·
IF 0
Fan Gao,Lanlan Yang,Jianguo Tang,Zhonglin Du,Yao Wang,Zhongyi Hu,Dongni Han,Linjun Huang,Laurence A. Belfiore
Pesticide residues in farm products create human health hazards. Hence, sensors that detect their existence are advantageous to address this critical problem. In this research contribution, fin-like CdSeS nanoplatelets were synthesized from solution by adjusting the selenium-to-sulfur molar ratio. Structural and photophysical properties were characterized via XPS, XRD, UV absorption, and fluorescence emission. CdSeS nanoplatelets exhibit a regular fin-like structure when the molar ratio of selenium-to-sulfur is 1:1. Fluorescence emission from these nanoplatelets occurs at two distinct wavelengths, characteristic of quantum dots. CdSeS fin-like nanoplatelets generate red-shifted fluorescence to 530 nm, which is useful to detect the popular organophosphorus pesticide dimethoate effectively at concentrations that range from 10–5 to 10–7 mol/L. Upon increasing dimethoate concentrations in solution, fluorescence intensity of CdSeS nanoplatelets decreases. Hence, direct sensing of dimethoate is feasible. Another important significance of this work is the use of low-toxicity and recyclable cadmium acetate instead of cancer-causing and nonrecyclable cadmium oxide, which will be helpful to reduce environmental pollution.
研究领域:
材料科学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
Materials Research Express
·
2019
·Vol. 6
·期 9
·095005
·
IF 1.449
Hui-Li Zhu,Qing-Yuan Cheng,Mei-Yan Liao,Zhi-Ling Zhang,Wei-Guo Cai,Jiao-Jiao Ma,Meng-Ying Sun,Ming-Xia Yu,Zhi-Quan Tian,Dai-Wen Pang
Ultra-small Bi2S3 nanoparticles are potential contrast agents for high-sensitive CT imaging. However, the expensive and harmful synthesis process limited their further application. Herein, an economical and low-toxic process was developed by employing S powders as sulfur precursors for large-scale preparation of ultra-small Bi2S3 nanoparticles. The as-prepared ultra-small Bi2S3 nanoparticles were modified with amine-terminated poly(ethylene glycol) (PEG) to produce water-soluble contrast agents. In vitro experiment results suggested that the ultra-small Bi2S3-PEG nanoparticles were low cytotoxic and superior contrast agents. In vivo experiments demonstrated that the ultra-small Bi2S3-PEG nanoparticles were preferred contrast agents for long-term and high-sensitive CT imaging.
研究领域:
医学影像学
·
纳米医学
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2014
·Vol. 20
·期 19
·5640-5648
·
IF 5.696
Yi-Fan Huang,Xin Zhou,Rong Zhou,Hong Zhang,Kai-Bin Kang,Min Zhao,Yong Peng,Qiang Wang,Hao-Li Zhang,Wen-Yuan Qiu
Graphical Transformers, quantum dots in disguise! The one-pot, green, and sustainable synthesis and functionalization of highly luminescent carbon quantum dots (CQDs), which can be easily transformed into flexible thin films for future roll-to-roll manufacturing (see scheme; TEOS=tetraethoxysilane, PVA=polyvinyl alcohol), is described. Aqueous CQDs were also applied for the in vivo imaging of zebrafish. The multifunctional CQDs provide a rich pool of tools for optoelectronic and biomedical applications. Photoluminescent carbon and/or silicon-based nanodots have attracted ever increasing interest. Accordingly, a myriad of synthetic methodologies have been developed to fabricate them, which unfortunately, however, frequently involve relatively tedious steps, such as initial surface passivation and subsequent functionalization. Herein, we describe a green and sustainable synthetic strategy to combine these procedures into one step and to produce highly luminescent carbon quantum dots (CQDs), which can also be easily fabricated into flexible thin films with intense luminescence for future roll-to-roll manufacturing of optoelectronic devices. The as-synthesized CQDs exhibited enhanced cellular permeability and low or even noncytotoxicity for cellular applications, as corroborated by confocal fluorescence imaging of HeLa cells as well as cell viability measurements. Most strikingly, zebrafish were directly fed with CQDs for in vivo imaging, and mortality and morphologic analysis indicated ingestion of the CQDs posed no harm to the living organisms. Hence, the multifunctional CQDs potentially provide a rich pool of tools for optoelectronic and biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 24
·5888
·
IF 4.2
Jixiang Wang,Yaowei Qin,Yue Ma,Minjia Meng,Yeqing Xu
With the development and improvement of analysis and detection systems, low-toxicity and harmless detection systems have received much attention, especially in the field of food detection. In this paper, a low-toxicity dual-emission molecularly imprinted fluorescence sensor (CdTe QDs@SiO2/N-CDs@MIPs) was successfully designed for highly selective recognition and visual detection of tetracycline (TC) in food samples. Specifically, the non-toxic blue-emission N-doped carbon dots (N-CDs) with high luminous performance acted as the response signals to contact TC via the covalent bond between amino and carboxyl groups. The red-emission CdTe quantum dots (CdTe QDs) were coated in silica nanospheres as stable reference signals, which effectively avoided the direct contact of CdTe QDs. Under optimum conditions, CdTe QDs@SiO2/N-CDs@MIPs had a rapid response within 1.0 min to TC, and the detection limit of CdTe QDs@SiO2/N-CDs@MIPs was calculated at 0.846 μM in the linear range of 0–140 μM. In complex environments, the CdTe QDs@SiO2/N-CDs@MIPs also exhibited excellent capabilities for the selective, rapid, and visual detection of TC. Furthermore, the accuracy of CdTe QDs@SiO2/N-CDs@MIPs to detect TC was verified by the HPLC method, and satisfactory results were obtained. Moreover, CdTe QDs@SiO2/N-CDs@MIPs showed a satisfactory recovery when measuring TC in milk and egg samples. This work provided an ideal approach for low-toxicity fluorescence sensor design and application.
研究领域:
分析化学
·
食品安全
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2016
·Vol. 63
·期 12
·977-984
·
IF 0.879
Rongjin Sun,Ting Yin,Peng Huang,Guo Gao,Joseph George Shapter,Yulan Shen,Jingjing Zhang,Daxiang Cui
In this paper, we report the development of a facile one-pot hydrothermal method for the controlled synthesis of monodispersed BaGdF 5 :Yb/Er nanoparticles for computed tomography (CT) and magnetic resonance (MR) imaging. The as-prepared nanoparticles have uniform size with a diameter of 20–25 nm. MTT tests show that the cell viability surpasses 90% even with a concentration of nanoparticles of 500 µg/mL, suggesting that the as-prepared BaGdF 5 :Yb/Er nanoparticles possess low toxicity. For both BaGdF 5 :Yb/Er nanoparticles and iopromide, the Hounsfield unit (HU) values increase with the their concentration. The slope of BaGdF 5 :Yb/Er nanoparticles is about 26.47, which is much higher than that of iopromide (16.98), indicating a better CT imaging effect. Interestingly, we find that the synthesized BaGdF 5 :Yb/Er nanoparticles exhibit paramagnetism. Our in vitro and in vivo imaging experiments demonstrate that the synthesized monodispersed BaGdF 5 :Yb/Er nanoparticles can serve as effective contrast agents for CT and MR imaging.
研究领域:
材料科学
·
医学影像学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Wen Li,Wen Deng,Xiaoqiang Fan,Fengjun Chun,Meilin Xie,Chao Luo,Shiyu Yang,Hanan Osman,Chuanqi Liu,Xiaotong Zheng,Weiqing Yang
All-inorganic perovskite nanocrystals have held great promise as incredibly effective luminescent materials and also have been synthesized efficiently by room temperature approaches. Toluene, a high toxicity solvent, is often used as a valid antisolvent in ligand-assisted reprecipitation strategy, which badly restricts the commercial application of this convenient method. Here, aiming to address the toluene toxicity issue, the low toxic tetraethyl orthosilicate (TEOS) is employed as a new alternative antisolvent to achieve high uniform and composition dependent luminescent all-inorganic perovskite Cs 4 PbX 6 (X = I, Br, Cl) combined with CsPbX 3 (X = I, Br, Cl) nanocrystals with tunable emission large-span wavelengths (626–437 nm) and remarkably narrow full width at half-maximum (FWHM) monochromaticity (down to 19 nm). Meanwhile, the ratio of CsBr to PbBr 2 is proved to be a critical factor to control the crystalline phase of the resulting perovskite nanocrystals . Additionally, one monochromatic light-emitting diode (LED) lamp with brightly pure green emission is fabricated based on bromine-based perovskite nanocrystals. This newly developed low toxicity antisolvent synthesis (LTaS) is suitable candidate for commercial production in an environmentally-friendly way, and the as-obtained perovskite materials with superior optical merits will be brought to the forefront of lighting and displays.
研究领域:
材料科学
·
光学工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2025
·
IF 6.5
Dongyan Duan,Hua Xie,Jiayi Jiang,Ping Yang,Zhiyuan Guo,Xiaoqiang Guo,Xingyu Chen,Qian Yao
Purpose Most absorption enhancers boost the oral absorption of drugs via increasing intestinal permeability. However, they often damage intestinal mucosa and induce inflammatory reactions. The aim of this study is to synthesize a new absorption enhancer, punicic acid ethyl ester (PAEE), with excellent absorption-prompting effect and low toxicity.Methods The structure of PAEE was confirmed by NMR, MS, IR and UV. Setting oleic acid (OA) as the control, the three forms of punicic acid (PA), ie, free PA, PAEE, and pomegranate seed oil, in which PA exists in the form of triglyceride, were formulated into nanoemulsions (NE). The stability, physiochemical properties of the oils and NE were compared. The permeation-enhancing effect was estimated by phenol red intestinal transport experiments. The potential damage on small intestines was assessed by biochemical assay and pathological section.Results Though the three forms of PA had various strength in enhancing intestinal permeability, the difference was not significant (p > 0.05). Moreover, the effect was notably stronger than that of OA (p < 0.05) and was inversely related to the density and required HLB value of the oils. Compared to the corresponding oils, the NE exhibited much weaker effect in prompting intestinal permeability. Oral administration of OA and OA NE for 10 d impaired intestinal mucosa and villi along with strong inflammatory reactions in the small intestines. In contrast, the oils from PA series and their NE did not induce obvious intestinal inflammation. PAEE and its NE hindered the release of cytokines and increased the ratio of intestinal villus length to crypt depth.Conclusion PAEE is a promising absorption enhancer with a strong permeability-prompting effect and mucosa-protecting capacity against intestinal inflammation. It provides a practical strategy to enhance the bioavailability of the drugs with poor biological membrane penetration.
研究领域:
药学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Shengran Li,Binggang Chen,Yangchun Qu,Xinxin Yan,Wenliang Wang,Xiaojing Ma,Bo Wang,Sanrong Liu,Xifei Yu
As one of the most promising therapeutic methods, gene therapy has been playing a more and more important role in treating disease due to its ultra-high therapy efficiency. Even if nonviral gene vectors represented by polycation, liposomal, dendrimers, and zwitterionic materials have made great progress in gene complexation, low immunogenicity, and biocompatibility, intracellular gene release with low toxicity is effectively still a bottleneck restricting the clinical application of gene therapy. We designed and synthesized a reactive oxygen species (ROS)-responsive dendrimer poly(amido amine)-N-(4-boronobenzyl)-N,N-diethyl-2-(propionyloxy)ethan-1-aminium (PAMAM-(B-DEAEP)16) as a gene vector whose potential can vary from positive to negative under the elevated ROS (H2O2) in cancerous cells. Dynamic light scattering results showed that the zeta potential of PAMAM-(B-DEAEP)16 decreased from +12.3 to −5 mV under 80 mM H2O2 in PBS buffer. The 1H NMR results demonstrated that the intermediate status of PAMAM-(B-DEAEP)16 was zwitterionic in ∼6 h because it consisted of the positive quaternary ammonium and negative carboxylic acid simultaneously before the ester bond was completely hydrolyzed. Gel retardation assay showed that PAMAM-(B-DEAEP)16 can condense DNA at above N/P = 1; then, PAMAM-(B-DEAEP)16 transfers to zwitterionic, which begins to continuously release DNA with the decrease in the positive charges and increase in the negative charges, and finally to negatively charged poly(amido amine)-propionic acid (PAMAM-PAc16) in the 80 mM H2O2. Fluorescence-labeled Cy-5 DNA indicated that PAMAM-(B-DEAEP)16 can enter into the cell completely in ∼4 h. The results showed that this compound we designed exhibited higher gene transfection efficiency and lower cytotoxicity than commercial PEI. This is the first time that the positively charged dendrimer was transferred to zwitterionic dendrimer under the stimuli of H2O2 and was successfully applied to gene delivery. Unlike all of the previous reports, we did not seek a compromise between the high gene transfection and low toxicity but find a new avenue to make the gene carrier not only have higher gene transfection efficiency but also exhibit lower toxicity by introducing stimuli-sensitive groups into the positively charged dendrimer to make it capable of adjusting the charge property according to the microenvironment. This study not only provides a good method to design materials for gene delivery but also opens a new perspective to understand the process of gene delivery.
研究领域:
生物医学工程
·
基因治疗
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）