ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yi-Min Zhu,Jie Tang,Xin Jin,Ting-Rui Pan,Yu Chang,Zhen-Guo Yang
To solve the problems of a conventional subtractive process for preparing conductive circuits, numerous alternative additive processes have been investigated, such as screen or inkjet printing, selective electroless plating, laser-induced forward transfer, etc. They all lead to a simpler procedure, less pollution, and finer line width but are still faced with difficulties like low conductivity and thickness, poor adhesion, and high cost. PDMS is a kind of material with low surface energy, leading to low adhesion with adhesive. Under these circumstances, a simple template transfer process for additively preparing conductive circuits is reported. The process to form the template includes the preparation of a photolithographic mask on the carrier copper foil and adsorption of PDMS anti-adhesion coating. Followed by metal deposition through electroplating on the template, the conductive circuits are transferred to the target substrate. Thus, the designed conductive circuits on various substrates including paper and cloth are formed. The template can be used again after being reimmersed into PDMS anti-adhesion coating. The components and the concentration of the coating are carefully discussed, and the mechanism of anti-adhesion is also researched by EIS and XPS. The copper circuits show a line width of 10 μm, a peeling strength of 7.11 N/cm, and a resistivity of 1.93 μΩ·cm, which is similar to that of bulk copper. With low pollution and cost, high versatility, and good electrical and adhesion performance, the template transfer process shows a good application prospect in the large-scale production of flexible electronics like sensors, RFID tags, etc.
研究领域:
材料科学
·
电子工程
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Xiaohang Yu,Weichen Meng,Yang Li,Xiliang Luo
Electrochemical biosensors with high sensitivity can detect low concentrations of biomarkers, but their practical detection applications in complex biological environments such as human serum and sweat are severely limited by the biofouling. Herein, a conductive hydrogel based on bovine serum albumin (BSA) and conductive carbon black (CCB) was prepared for the construction of an antifouling biosensor. The BSA hydrogel (BSAG) was doped with CCB, and the prepared composite hydrogel exhibited good conductivity originated from the CCB and antifouling capability owing to the BSA hydrogel. An antifouling biosensor for the sensitive detection of cortisol was fabricated by drop-coating the conductive hydrogel onto a poly(3,4-ethylenedioxythiophene) (PEDOT) modified electrode and further immobilizing the cortisol aptamer. The constructed biosensor showed a linear range of 100 pg mL −1 - 10 μg mL −1 and a limit of detection of 26.0 pg mL −1 for the detection of cortisol, and it was capable of assaying cortisol accurately in complex human serum. This strategy of preparing antifouling and conductive hydrogels provides an effective way to develop robust electrochemical biosensors for biomarker detection in complex biological media.
研究领域:
生物传感器
·
电化学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Lihua Chen,Mingchao Liu,Yan Tang,Chuangfu Chen,Xingxing Wang,Zhiqiang Hu
Gold magnetic particles as a new carrier of disease diagnosis probes have attracted wide attention but encountered a bottleneck. That is, the interfacial properties of gold magnetic particles are susceptible to the influence of nonspecific biological molecules in actual diagnostic samples. Here, a novel nanoparticle made by the covalent attachment of polyethyleneimine and hyperbranched polyether polyol onto the gold shell surface of a magnetic bead demonstrated not only low fouling properties but also excellent stability in a variety of external environments, especially in complex biological systems. Most importantly, in its application as the probe for sensitive and selective fluorescence detection of high-risk human papillomavirus (HPV) genotypes 18, 16 in buffer, even in 100% serum, a good linear correlation with the concentration of HPV18/16 target DNA ranging from 5 nM to 1 μM was shown with the low detection limits. To our knowledge, this is one of the few successful examples of direct application of magnetic beads to the detection of disease markers in whole serum, suggesting that this material has good commercial potential and value.
研究领域:
生物医学工程
·
分子诊断学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 51
·期 13
·6379-6388
·
IF 2.302
Gao Nengwen,Fan Yiqun,Quan Xuejun,Cai Yongwei,Zhou Dewen
Growing environmental concerns have fueled the need for efficient separation of oil–water mixtures. Membranes that separate oil–water mixture based on contrasting wetting properties have recently received significant attention. Here, hydrophobic and oleophilic ceramic membranes have been successfully prepared for the application of water-in-oil emulsion separation. The membranes were fabricated by modification the ZrO 2 membrane with hexadecyltrimethoxysilane (HDTMS). SEM, EDS, and pore size measurements depict that HDTMS has been grafted onto the ZrO 2 membrane surface without changing the membrane morphology and pore structure. Contact angle measurements confirm that HDTMS modification improves the membrane hydrophobicity and oleophilicity. The modified membrane was thermally stable to 220 °C, and had good chemical stability in a series of organic solvents, even under high-speed scouring. The antifouling performance of the modified membrane was assessed via the filtration of water-in-oil emulsions. The modified membranes showed higher oil flux and higher water rejection compared with the unmodified membranes. The modified membrane can separate all the water droplets from the water-in-oil emulsions. The hydrophobicity of the grafted HDTMS chains was considered to be responsible for reducing the water droplet adsorption on the membrane surface.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 141
·期 23
·e55478
·
IF 2.7
Wei Zhao,Wenxia Zhao,Xinwei Li,Xin Liu,Kaihong Hui,Yifan Song,Huaijun Chen,Yubo Cui,Qian Zhu,Jing Liu,Liang Qiao,Yani Yan
In the present study, the low pollution acidic potassium permanganate system has been used in the study of its micro-etching effect on polycarbonate-acrylonitrile butadiene styrene (PC-ABS). Prior to micro-etching using a N methyl pyrrolidone (NMP)-glycol butyl ether solution was used as the swelling system of PC-ABS. Reasonable swelling conditions were determined, a 2-min expansion period at a temperature of 35°C with an NMP concentration of 80%. After the swelling processes, the effects of the volume ratio of V H 2 O : V H 2 S O 4 , micro-etching temperature, and micro-etching duration on the PC-ABS surface morphology, surface roughness, surface hydrophilicity, and peel strength, have been investigated. It was finally determined that the best micro-etching effect could be obtained by reacting at V H 2 O : V H 2 S O 4 = 1:2 and a temperature of 60°C for 25 min. The surface of the PC-ABS copolymers was characterized by x-ray photoelectron spectroscopy, atomic force microscopy, and Fourier-transform infrared spectroscopy. The results showed that the micro-etching process changed the morphology of the PC-ABS surface and generated cavities. In addition, the surface roughness increased from the initial R a = 9.69 to R a = 197 nm. Also, the water contact angle on the PC-ABS surface decreased from 92.2° to 28.6°, and the peel strength increased to 0.98 kN/m.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 3
·1-9
·
IF 5.705
Wang Wei,Jayachandran Silambarasan,Li Mengru,Xu Shenghao,Luo Xiliang
An electrochemical biosensor for the detection of microRNA was prepared via chemical grafting of a Methylene Blue labeled reporter (MB-Rep) duplex onto a nanostructured surface that was obtained by electrodeposition of cobalt oxide and poly(o-phenylenediamine). This is followed by the attachment of hyaluronic acid and gold nanoclusters. In the presence of the target (microRNA), the probe-target duplex and the MB-Rep hairpin are formed. These will displace the labeled reporter from the sensor surface, and this results in a decrease of the amperometric signal for MB at a typical working voltage of −0.28 V (vs. Ag/AgCl). The electrode modified with hyaluronic acid possesses a large electroactive surface area and an excellent antifouling property. This makes it useful for ultrasensitive quantitation of microRNA even in complex biological media. The sensor has a linear response in the 100 f. to 0.1 μM microRNA concentration range, and a 33.3 f. detection limit. It was successfully applied to the determination of microRNA in cancer cells.
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研究领域:
生物化学
·
电化学
·
纳米技术
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Zhenying Xu,Rui Han,Nianzu Liu,Fengxian Gao,Xiliang Luo
It remains a very challenging problem for electrochemical biosensors to assay disease markers in real complex biological media on account of the severe nonspecific adsorption. Herein, a novel strategy for the construction of sensitive and antifouling biosensors capable of detecting a typical tumor marker, carcinoembryonic antigen (CEA), in complex biological media was developed through the convenient one-step copolymerization of polydopamine (PDA) and poly(sulfobetaine methacrylate) (PSBMA). In the presence of the copolymerized PDA and PSBMA, sensing probes (CEA aptamers) with thiol groups can be attached to the PDA via the Michael addition reaction, while the zwitterionic polymer PSBMA ensures antifouling capability of the sensing interface. The prepared electrochemical biosensor showed satisfying assaying performances for the detection of CEA, with a linear range of 0.01-10 pg mL −1 , and a low limit of detection (LOD) down to 3.3 fg mL −1 . More importantly, the existence of PSBMA in the sensing interface enabled the electrochemical biosensor to detect CEA in complex biological media with satisfying antifouling effect, indicating the feasibility of such biosensors based on copolymerized PDA and PSBMA for practical assaying of human serum samples.
研究领域:
生物传感器
·
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Shiyi Zhao,Yingxia Zhou,Lai Wei,Lihua Chen
Based on the successful design and preparation of Fe 3 O 4 @ [email protected] glycol (PEG)@chondroitin sulfate (CS) nanoparticles , a novel electrochemical biosensor was elaborately developed for MO determination by the drop coating method. This biosensor demonstrates ultra-low fouling properties in complex biological systems, especially in whole serum. Only 2.40% of the differential pulse voltammetry (DPV) signal changes are shown in the resistance test of electrode interface to 100% goat serum. The constructed biosensor also presented a good selectivity (DNA samples for M1, M2 and M3give a response around 14.2–21.4%of that obtained by the target analyte), reproducibility (the relative standard deviation (RSD) is only 4.82%) and storage stability (93.2% of the initial response is maintained after 20 day). Moreover, this biosensor exhibits high analytical performance with a wide line arranges (10 −17 M ∼ 10 −12 M) in buffer, even in 100% goat serum. In both cases, a low limit of detection (LOD) of 3.3 aM(S/N = 3)is showed. To our knowledge, a few examples reported the application of electrochemical biosensors to the direct ultrahigh sensitive determination of MO, especially in whole serum.
研究领域:
电化学
·
生物传感器
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemElectroChem
·
2020
·Vol. 7
·期 9
·2081-2086
·
IF 4.154
Xinru Li,Lin Zhou,Jialian Ding,Lei Sun,Bin Su
Graphical Direct detection : A low-fouling hydrogen peroxide (H 2 O 2 ) electrochemical sensor, which consists of a silica nanomembrane (SNM) as antifouling layer and Pt nanostrutures as catalysts is presented. The SNM can effectively protect the substrate electrode from severe fouling. Thus, the Pt@SNM modified electrodes can be direct used for detection of H 2 O 2 in biosamples and living tissues without any pre-treatments. Hydrogen peroxide (H 2 O 2 ) is an important molecule that is widely used or exists in foods, medicines, detergents and human body. Therefore, fast and accurate detection of H 2 O 2 is highly important in food security, disease diagnosis, etc. In this work, we report the fabrication of platinized silica nanoporous membrane (Pt@SNM) electrodes for the detection of H 2 O 2 in complex in vitro and in vivo environments. The porous structure of SNM can effectively confine the electrodeposition of Pt nanostructures and meanwhile prevent the adsorption of fouling species on the underlying electrode. In comparison with conventional electrodes for H 2 O 2 detection, the current one exhibited excellent antifouling ability and worked well in complex real samples. As we demonstrated that the Pt@SNM modified carbon fiber microelectrode could work pretty stably in cortex of rat brain up to 90 min. We believe that this methodology can be extended for electrochemical detection of many other species in strongly fouling media.
研究领域:
电化学
·
生物传感器
·
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Weijun Zhang,Liucheng Zhang,Kunxi Zhang,Wenguo Cui,Jingbo Yin
Vascularization is of great importance for the adipose tissue engineering. Although shape memory scaffolds have exhibited advantages in minimally invasive approach, lacking angiogenesis property is the major barrier of such smart material applied for vascularized adipose construction. Here, low-fouling shape memory scaffolds as the advanced carrier of stem cells to promote the vascularized adipose regeneration is innovatively developed based on crosslinking poly(ω-pentandecalactone) (PPDL) with poly(L-glutamic acid)- g -poly(ε-caprolactone) (PLGA- g -PCL), and modification by dopamine modified poly(ethylene glycol) (DA-PEG). The scaffolds exhibited adipose-mimicking elastic moduli through PPDL adjustment. More importantly, the scaffold with DA-PEG low-fouling coating could prevent adipose derived stem cells (ASCs) adhesion, while driving ASCs to form aggregates. In vitro angiogenesis and adipogenesis analysis revealed that the low-fouling shape memory scaffold exhibited better performance in the expression of angiogenic and adipogenic factors. In vivo evaluation showed that scaffold carrying ASCs aggregates could promote regeneration of vascularized adipose. Therefore, these results have presented a newly low-fouling shape memory scaffold with great potential in the application of vascularized adipose construction.
研究领域:
组织工程
·
生物材料科学
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Qingyu Yu,Hong Sun,Linhua Zhang,Lijie Jiang,Lei Liang,Chaojie Yu,Xiaoru Dong,Bingyan Guo,Yuwei Qiu,Jingwu Li,Hong Zhang,Fanglian Yao,Dunwan Zhu,Junjie Li
Postoperative peritoneal adhesion is a serious clinical complication. Various hydrogel barriers have been developed to prevent peritoneal adhesion. However, it remains a challenge to design a hydrogel with desirable physicochemical properties and bioactivities. In this study, a zwitterionic polysaccharide-based multifunctional hydrogel is developed using epigallocatechin-3-gallate (EGCG) to prevent postoperative abdominal adhesion. This hydrogel is simple to use and has desirable properties, such as excellent injectability, self-healing, and non-swelling properties. The hydrogel also has ultralow fouling capabilities, such as superior bactericidal performance, cell and protein adhesion, and low immunogenicity resistance. Moreover, the hydrogel exhibits good antioxidant activity, which is attributed to the integration of EGCG. Furthermore, the detailed mechanism from in vivo and in vitro experimental studies illustrates that hydrogel compositions can synergistically prevent adhesion formation through multiple pathways, including anti-inflammatory and antioxidant capabilities and inhibition effects on the mesothelial-mesenchymal transition (MMT) process induced by transforming growth factor (TGF-β). In summary, this zwitterionic multifunctional hydrogel has great potential to prevent postoperative adhesion formation in the clinical setting.
研究领域:
生物医学工程
·
材料科学
·
药剂学
·
外科手术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）