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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2023 · IF 6.1

Ultrasound Treatment Improves Fruit Quality of Postharvest Blood Oranges (Citrus sinensis L. Osbeck): Anthocyanin Enrichment and Its Biosynthesis

Jin Chen,Yanru Zhang,Feifei Liu,Jianle Chen,Yujing Sun,Xingqian Ye,Donghong Liu,Huan Cheng

This study was to investigate the effects of different nonthermal treatments on quality attributes, anthocyanin profiles, and gene expressions related to anthocyanin biosynthesis during low-temperature storage, including pulsed light (PL), magnetic energy (ME), and ultrasound (US). Among these treatments, 1 min US treatment was the most effective method for improving fruit quality and increasing total anthocyanin contents (by 29.89 ± 3.32%) as well as individual anthocyanins during low-temperature storage of 28 days. This treatment resulted in high color intensity, intact cellular architectures, and positive sensory evaluation. In contrast, PL and ME treatments displayed negative effects on quality improvement, leading to the destruction of cell architectures and inhibiting anthocyanin levels. Furthermore, qPCR analysis revealed that the structural genes (C4H, CHS1, CHS2, CHI, F3H, ANS, and GST) related to anthocyanin biosynthesis and transport were the target genes and upregulated in response to the cavitation effect of US treatment.

研究领域: 食品科学 · 植物生理学 · 分子生物学
✓ 已验证 DOI PubMed 高影响因子
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Foods · 2022 ·Vol. 11 ·期 11 ·1642 · IF 5.561

Impact of Low-Temperature Storage on the Microstructure, Digestibility, and Absorption Capacity of Cooked Rice

Hui Li,Bingxiao Liu,Kezia Bess,Zhengxuan Wang,Mingcai Liang,Yan Zhang,Qiong Wu,Lin Yang

This study examined the effects of low-temperature storage on the microstructural, absorptive, and digestive properties of cooked rice. Cooked rice was refrigerated and stored at 4 °C for 0.5, 1, 3, 5, and 7 days, as well as frozen and preserved at −20, −40, and −80 °C for 0.5, 1, 3, 5, 7, 14, 21, and 28 days. The results indicated that the stored rice samples generally exhibited a higher absorption capacity for oil, cholesterol, and glucose than the freshly cooked rice. In addition, after storage, the digestibility of the cooked rice declined, namely, the rapidly digestible starch (RDS) content and estimated glycemic index (eGI) decreased, whereas the slowly digestible starch (SDS) and resistant starch (RS) content increased. Moreover, the increment of the storage temperatures or the extension of storage periods led to a lower amylolysis efficiency. Scanning electron microscopy (SEM) analysis indicated that storage temperature and duration could effectively modify the micromorphology of the stored rice samples and their digestion. Moreover, microstructural differences after storage and during simulated intestinal digestion could be correlated to the variations in the absorption capacity and digestibility. The findings from this study will be useful in providing alternative storage procedures to prepare rice products with improved nutritional qualities and functional properties.Keywords:cooked rice;low-temperature storage;microstructure;absorption capacity;digestibility

CAS / MDL: 9032-08-0
研究领域: 食品科学 · 营养学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Journal of Future Foods · 2023 · IF 0

Colorimetric indicator based on zwitterionic anti-freezing hydrogel and alizarin for visual monitoring of salmon fillets freshness

Yanan Liu,Huan Li,Jian Chen,Jiesi Feng,Renjie Lian,Linglin Fu,Yanbo Wang

ABSTRACT Indicating hydrogels have shown the advantages of being fast, sensitive, and real-time in demonstrating the freshness of aquatic products due to their noticeable color change. In this work, an anti-freezing hydrogel embedded with pH-sensitive alizarin was developed as an original colorimetric indicator for monitoring the corruption of aquatic products at low temperatures. The anti-freezing hydrogel was prepared from 2-carboxyethyl acrylate, [2-(methacryloyloxy)ethyl]-trimethylammonium chloride, and 2-hydroxyethyl methacrylate monomers with triethylene glycol dimethacrylate as cross-linking agent. The hydrogel showed excellent anti-freezing capability, which was further incorporated with the pH-sensitive dye alizarin to gain the indicating capability. When applied in the indication of the freshness of salmon under low-temperature storage, it was found that the color changes of the indicator corresponded with the total volatile basic nitrogen contents of salmon, the correlation coefficient of which was 0.959 6. These results indicated the application potential of the hydrogels as freshness indicators during low-temperature storage or cold-chain transportation.

研究领域: 材料科学 · 食品科学 · 化学工程
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2021 · IF 4.952

Phospholipid composition and fat globule structure change during low temperature storage of human milk

Wei Wei,Jin Cheng,Jie Yang,Chen Chen,Qingzhe Jin,Jie Song,Xingguo Wang

The nutritional profile of human milk varies significantly during storage, and the impact of storage conditions on phospholipid (PL) composition and fat globule structure is not well studied. This study aimed to investigate the changes in human milk PL composition and human milk fat globule (HMFG) structure during long-term storage, at 4 °C, −20 °C and −80 °C for 30, 60, 90 and 120 days. The PLs in human milk were quantified using 31 P NMR, and the microstructure of HMFG was observed using multi microscopes. After 120 days of storage, the total PLs decreased from 19.1 μmol/100 mL milk to 4.8 μmol/100 mL, 8.2 μmol/100 mL and 10.3 μmol/100 mL at 4 °C, −20 °C and −80 °C, respectively. Glycerophospholipid showed a higher lipolysis rate than sphingomyelin . PLs on milk fat globule membranes degraded during storage, which caused the release of internal triacylglycerols and the flocculation of fat globules at the broken membrane.

CAS / MDL: 3436-44-0 85187-10-6
研究领域: 营养学 · 食品科学 · 生物化学
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY · 2021 ·Vol. 57 ·期 1 ·516-524 · IF 3.713

NC-1 coating combined with 1-MCP treatment maintains better fruit qualities in honey peach during low-temperature storage

Wangshu Zhang,Luyang Jing,Huiyun Chen,Sheng Zhang

Summary The effect of carnauba wax-based nanoemulsion coating-1 (NC-1) combined with 1-methylcyclopropene (1-MCP) treatment on the storage of honey peach fruit ‘Yulupantao’ for 28 days under 0 ± 0.5 °C, and 90% RH conditions was studied. The results showed that NC-1 or 1-MCP treatment alone and in combination were proved to be more effective in maintaining fruit qualities compared to the control group with no treatment. However, the application with the combination of NC-1 and 1-MCP treatment had the most significant effect on reducing mass loss and decay rate, maintaining firmness, total soluble solids (TSS), ascorbic acid (AsA) and titratable acid (TA) contents, suppressing malondialdehyde (MDA) accumulation and conductivity ascend. Moreover, the activities of peroxidase (POD) were maintained, and polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL) were effectively suppressed in NC-1, 1-MCP and combined treatment than that of control. Therefore, the combination of NC-1 and 1-MCP treatment could be an efficient way to maintain the qualities of peach fruit during low-temperature storage after harvest.

CAS / MDL: 544-63-8 112-80-1
研究领域: 食品科学 · 植物生理学
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Plant Gene · 2021 · IF 0

High-throughput sequencing analysis reveals effects of short-term low-temperature storage on miRNA-mediated flavonoid accumulation in postharvest toon buds

Hu Zhao,Xiaopu Shi,Cheng Shen,Caifeng Chen,Jiyuan Liu,Changqing Qu

Toon bud is rich in polyphenols and flavonoids, which not only have nutritional value but also have health benefits. However, the molecular mechanisms of flavonoid accumulation during storage remain unclear. In our report, small RNA libraries from toon buds were synthesized after three storage period (0, 3, and 5 days) at 4 °C. Overall, 303 homologous sequences with known microRNA (conserved miRNAs homology) and 19 putative novel miRNAs were obtained using Illumina sequencing technology. Furthermore, the potential target unigenes of these miRNAs were predicted. Of the predicted miRNAs and their target unigenes, 17 conserved miRNAs and their target unigenes were confirmed by quantitative real-time PCR (qRT-PCR). Exactly 11 miRNAs involved in the flavonoid pathway and 6 miRNAs targeting transcription factors displayed significant correlation with flavonoid accumulation in toon buds. The findings will be helpful for better understanding flavonoid accumulation in toon buds during storage.

研究领域: 植物科学 · 分子生物学 · 食品科学
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CHEMICAL ENGINEERING JOURNAL · 2024 · IF 13.3

Long-term cold storage of platelets for transfusion based on zwitterionic magnetic microgels

Yihao Fu,Yiming Ma,Yunqing Tian,Liming Zhang,Jing Yang,Lei Zhang

Hypothermic preservation of platelets at 22 °C with an agitation process realizes their rapid transfusion to treat hemorrhage and thrombocytopenia in the clinic. However, platelets suffer from activation, aggregation, and bacterial contamination during storage, resulting in a limited shelf life of 5 days. Cold storage in a refrigerator can effectively prevent bacterial growth, but this condition is challenging to preserve platelets, owing to cold-induced reactive oxygen species (ROS) overproduction or platelet desialylation. Here, we design a zwitterionic magnetic microgel assembly to preserve platelets for a long time without agitation at 4 °C. The microgel assembly can scavenge cold-induced ROS and provide a super-hydrophilic 3D environment to isolate platelets, preventing platelet activation, aggregation, and desialylation. Moreover, the antifouling property of microgel assembly at 4 °C can ensure the anti-bacterial environment for platelets. As a result, the cold storage time of platelets significantly extended to 7 days. This work serves as a springboard for the development of platelet-based clinical applications.

研究领域: 生物医学工程 · 血液学 · 材料科学
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SEPARATION AND PURIFICATION TECHNOLOGY · 2025 · IF 9

Low-temperature ammonia adsorption-desorption performance of Al2O3-supported, graphite-casted metal chloride pellets

Haoyu Yan,Peng Wang,Pu Huang,Cheng Shen,Jian Sun,Tao Song

Ammonia (NH 3 ) is widely recognized as a carbon-free hydrogen carrier with high energy density, making it a promising energy vector for sustainable energy systems. However, its efficient storage and release remain technical challenges. In this study, the low-temperature NH 3 adsorption-desorption performance of four pure metal chlorides (CaCl 2 , MgCl 2 , MnCl 2 , and CuCl 2 ) was systematically evaluated. Among them, CuCl 2 and MnCl 2 exhibited superior NH 3 uptake capacities, but also suffered from volume expansion and particle agglomeration during repeated cycling. To overcome these limitations, composite sorbent pellets were developed by supporting metal chlorides on Al 2 O 3 via a graphite-casting method. The resulting CuCl 2 -based composite pellet achieved an optimized balance of high NH 3 adsorption capacity (∼ 0.36  g NH 3 /g), excellent regeneration efficiency (> 96 %), and adequate mechanical strength. Excessive CuCl 2 loading reduced both regeneration efficiency and mechanical stability, while lower loading enhanced active phase dispersion and sorbent performance. 30 wt% CuCl 2 offered the best performance in terms of adsorption, regeneration, and structural durability. Additionally, the optimized Cu 30 -Al-C sorbent maintained stable NH 3 adsorption capacity over 20 cycles, confirming excellent reusability. The enhanced performance was attributed to the synergistic effects of Al 2 O 3 support and the hydrophobic nature of the graphite-casting process, which enabled uniform distribution of active compound and mitigated structural deterioration during cycling. These results demonstrate the feasibility of graphite-casted, Al 2 O 3 -supported metal chlorides as structurally stable and regenerable NH 3 sorbents for low-temperature ammonia storage applications.

CAS / MDL: 7782-42-5
研究领域: 储能 · 材料科学 · 化学工程 · 可持续能源 · 吸附技术
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ACS Applied Nano Materials · 2024 · IF 5.3

Lecithin/Cholesterol/Tween 80 Liposomes for Co-Encapsulation of Vitamin C and Xanthoxylin

Ruijuan Wang,Chunliu Ma,Haitao Yan,Hailiang Zhao,Pu Wang,Shoubing Zhang,Jianwei Ju,Shuyan Yu,Zhigang Yin

Co-encapsulated hydrophilic vitamin C (Vc) and hydrophobic xanthoxylin (GX-50) liposomes (L-Vc-GX-50) were prepared using ethanol injection and ultrasonic methods and characterized by dynamical light scattering, ζ-potential, cryogenic transmission electron microscopy, UV–vis spectroscopy, Fourier transform infrared spectroscopy, antioxidant capacity, tyrosinase activity, and in vitro permeation measurements. L-Vc-GX-50 are formed by the assembly of egg yolk lecithin, cholesterol, and tween 80, with Vc and GX-50 encapsulated in their hydrophilic and hydrophobic cavities, respectively. By considering the hydrodynamic radius (Rh), ζ-potential, and encapsulation efficiency (EE) of L-Vc-GX-50, the optimum formulation was determined to be egg yolk lecithin/cholesterol/tween 80/GX-50 or Vc (15:3:3:1, w/w) with phosphate buffer (pH 6.8, 0.01 M). The as-prepared L-Vc-GX-50 have an average Rh of 114 nm, a negative ζ-potential (−23.63 mV), a high EE of Vc (73.3%) and GX-50 (87.4%), and a unilamellar vesicle structure. Compared co-encapsulated L-Vc-GX-50 with single encapsulated Vc or GX-50 liposomes (L-Vc or L-GX-50), the EE of Vc in L-Vc-GX-50 is obviously higher than that of L-Vc, while the EE of GX-50 in L-Vc-GX-50 is similar to that in L-GX-50; the antioxidant capacity and tyrosinase inhibition activity of L-Vc-GX-50 are higher than those of L-GX-50 or L-Vc. The in vitro permeation data indicate that the final cumulative permeation amount of Vc and GX-50 in L-Vc-GX-50 can reach around 2.1 and 1.3 mg/cm2, respectively. The release kinetics fitting data show that the release kinetics of L-Vc-GX-50 follows the Korsmeyer–Peppas model and that the release of active ingredients is mainly dominated by the synergistic effect of diffusion release and rupture release. Moreover, L-Vc-GX-50 has good low-temperature storage stability, and the storage stability of GX-50 in L-Vc-GX-50 is significantly improved compared to L-GX-50. The main objective of this study is to improve the instability of Vc and the insolubility of GX-50 in cosmetics.

CAS / MDL: 9005-65-6
研究领域: 药剂学 · 纳米技术 · 化妆品 · 药物递送系统 · 分析化学 · 生物物理学
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FOOD RESEARCH INTERNATIONAL · 2021 · IF 6.475

Fabrication and characterization of oil-in-water pickering emulsions stabilized by ZEIN-HTCC nanoparticles as a composite layer

Mengna Cao,Chengzhi Liu,Jieyu Shi,Fangfang Ni,Jiaming Qi,Qing Shen,Min Huang,Gerui Ren,Shiyi Tian,Quanquan Lin,Xiaoxin Lu,Qunfang Lei,Wenjun Fang,Hujun Xie

In this work, the ZEIN-HTCC nanoparticles formed by zein and N-(2-hydroxy)propyl-3-trimethylammonium chitosan chloride (HTCC) were used as stabilizers to prepare oil-in-water (O/W) Pickering emulsions. The preparation conditions including shearing time, volume fraction of corn oil, mass ratio of ZEIN:HTCC and total concentration of ZEIN-HTCC of emulsions were optimized by measuring the droplet size, zeta potential, PDI and surface tension of emulsions. The ZEIN-HTCC emulsions are stable at the pH range of 4–9 and in the low salt ion concentrations up to 0.2 mol L −1 , and can keep stable up to 21 d during low temperature storage. Fourier transform infrared spectroscopy (FTIR), the confocal laser scanning microscope (CLSM) and scanning electron microscopy (SEM) were used to analyze the interaction between emulsion components, revealing that zein and HTCC form a composite layer by flocculation to adsorb on the surface of oil droplets, thus preventing emulsion droplets from aggregation. This novel, long-term stable, surfactant-free, and edible zein-based Pickering emulsion could be used as potential carriers for lipophilic nutrients delivery.

CAS / MDL: 7647-14-5 7758-02-3
研究领域: 食品科学 · 胶体与界面化学 · 纳米技术 · 材料科学
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