STARCH-STARKE
·
2016
·
IF 1.523
Jianhua Liu,Hu Li,Jinhong Wu,Fan Xie,Juan Zhang,Zhengwu Wang
Phosphoryl oligosaccharides (POs) are a new type of functional oligosaccharide that promote the absorption of calcium. Canna edulis Ker (edible canna) starch contains a small amount of phosphorus and may be a source in the production of POs. Herein, the POs fraction was separated from Canna edulis Ker starch for the first time using enzymatic reactions that produce isomalto-oligosaccharides (IMOs). Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and high-performance anion exchange chromatography (HPAEC) were used to determine the presence and composition of the isolated POs. The POs that were obtained from Canna edulis Ker starch were mainly composed of phosphoryl maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose. These findings provide new insight into the simultaneous production of IMOs and POs from Canna edulis Ker starch by enzymatic reaction.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 189
·期 2
·608-625
·
IF 2.14
Zhou Ruolin,Ren Zhiqing,Ye Jun,Fan Yawei,Liu Xiaoru,Yang Jianyuan,Deng Ze-Yuan,Li Jing
Soybean dregs fermented by Neurospora crassa is a typical traditional food in Gannan district of China. In this study, in vitro imitated gut fermentation was carried out to evaluate whether the oligosaccharides from this fermented soybean dregs had potential prebiotic properties. 11.91% of oligosaccharides were extracted from the fermented soybean dregs at the optimized condition which of 1:25 for ratio of soybean dregs (g) to 50% ethanol (ml), 90 min of extracted duration at 70 °C for twice. The soybean dreg oligosaccharides (SBOS) were progressively purified with Sevag method and on columns filled with AB-8 macroporous resin, and then identified as cellobiose by HPLC-ESI-MS and FT-IR. Oligosaccharides of soybean dregs with 800 mg/L significantly decreased pH value ( p < 0.05) and ammonia N concentration ( p < 0.05), and increased short chain fatty acid (SCFA) level ( p < 0.05) in imitated gut fermentation compared with control group. It was shown that this fermented soybean dregs could be a potential prebiotic food.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ang-Xin Song,Yu-Heng Mao,Ka-Chai Siu,Jian-Yong Wu
The bifidogenic effects of exopolysaccharide (EPS) of a medicinal fungus ( Cordyceps sinensis ) and a well-known food polysaccharide konjac glucomannan (KGM) with different molecular weight (MW) ranges were evaluated through in vitro experiments in liquid cultures of Bifidobacteria . Native EPS and KGM were partially degraded with power ultrasound (US) to improve the water solubility, and further hydrolysed with trifluoroacetic acid to much lower MW. The acid-hydrolysed fractions (EPS-AH and KGM-AH) supported the growth of all five tested bifidobacterial species, while the US-degraded high MW fractions, EPS-US and KGM-US, could only slightly support the growth of some species. All EPS fractions increased the acetic acid production of most bifidobacterial species. Most remarkably, the high MW EPS-US, EPS-AH and KGM-US fractions significantly enhanced the cell viability with much higher colony forming unit (CFU) counts, suggesting a protective effect of these high MW polysaccharides for the bacterial survival . The results have shown that MW was a significant factor on the bifidogenic properties of partially degraded EPS and KGM.
研究领域:
微生物学
·
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 90
·期 1
·e17647
·
IF 3.2
Jingyu Gao,Fan Wu,Mingzhu Yan,Xuejiang Wang,Yan Chi,Yu Zhang,Yu Peng,Mo Li,Yuanying Ni,Xin Wen
Brown seaweed oligosaccharides (BSO) have demonstrated potential as nutraceuticals with cholesterol-lowering, anti-obesity, and anti-constipation properties. In this study, we initially examined how BSO impact body weight, blood lipid levels, and adipose tissue in a rat model of obesity induced by a high-fat diet. Our findings revealed that BSO administration significantly attenuated body weight gain, ameliorated dyslipidemia, and reduced visceral adiposity. Additionally, we evaluated the prophylactic efficacy of BSO in a loperamide hydrochloride-induced constipation mouse model by assessing defecation parameters and gastrointestinal motility. Our results indicated that BSO intervention significantly increased fecal pallet output and fecal water content, as well as improved intestinal transit. In conclusion, this study lays the foundation for a deeper understanding of how BSO may influence obesity and constipation, offering novel insights into its potential safe and efficacious clinical applications.
研究领域:
营养学
·
代谢生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2025
·
IF 2.5
Lin Ge,Ruobing Jia,Yingying Liu,Lingling Zhan
Soybean contains anti-nutritional factor raffinose oligosaccharide (RFOs), which can cause flatulence, gastrointestinal dysfunction and low feed utilization rate. However, α-galactosidase can hydrolyze raffinose oligosaccharides (RFOs). Therefore, in this study, a novel α-galactosidase from Thermoclostridium stercorarium subsp. thermolacticum DSM 2910 (TstGal) which can hydrolyze raffinose was cloned, expressed, purified, and characterized. The gene fragment size is 2208 bp, and the enzyme TstGal consists of 736 amino acids. Under the optimum culture conditions, the maximum enzyme activity of the target protein TstGal was 23.8 U/mL. The enzyme was purified 11.3-fold by Ni-NTA agarose resin with an overall recovery of 51.4 % and specific activity of 9.0 U/mg, and its relative molecular weight was about 85 kDa. The optimal temperature of TstGal was 70 °C, and it exhibited excellent thermal stability at 60 °C. Furthermore, The TstGal had the highest activity at pH 6.5 and good pH stability at pH 5.0–7.5. Besides, the enzyme has a good sugar tolerance to galactose and sucrose. In addition, K + and Fe 2+ could significantly enhance the enzyme activity at a concentration of 5 mM. The values of K M , V max , k cat , and k cat /K M for p NPGal were found to be 0.507 mM, 13.979 U/mg, 19.735 s −1 , and 38.895 s −1 mM −1 , respectively. Under the optimum conditions, the maximum hydrolysis rate of raffinose by TstGal reached 99.8 %, which shows that the enzyme had potential application value in food and feed industry.
研究领域:
生物化学
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Shengqi Jiang,Huiyu Han,Madina Aitmagambetova,Aidana Rakhimova,Lin Li,Rong Bai,Chunjie Yang,Xiang Ye,Siying Wang,Jing Xi,Wu Ding
This study aimed to investigate the effects of oligosaccharides (xylooligosaccharides (XOS), fructooligosaccharides (FOS), galactooligosaccharides (GOS), and isomaltooligosaccharides (IOS)) with different structures and molecular weights on the glycosylation modification of pigskin collagen (CO). Glycosylated CO was prepared via the Maillard reaction, and its molecular structure, physicochemical, and functional properties were analyzed using various analytical techniques. Results indicated increased glycosylation significantly altered the α-helix and random coil content, intrinsic fluorescence intensity, particle size, and ζ-potential of CO ( P < 0.05). These modifications enhanced protein-protein and protein-water interactions, improving solubility, thermal stability, rheological properties, and creating a more uniform gel network structure. XOS, composed of the smaller molecular weight five-carbon aldose unit (xylose), exhibited the most significant effects, including enhanced thermal stability, water retention, and gel strength. However, the Maillard reaction-induced glycation browning caused a slight negative impact on the color of CO gels. In conclusion, oligosaccharide glycosylation significantly enhances the functional properties of pigskin CO, providing a promising strategy for its high-value utilization.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Jingfeng Li,Lifang Chen,Zhiyun Meng,Guifang Dou
Chitosan has multiple biological activities, but a sensitive and rapid characterization method is yet to be developed for its further application. This study presented an optional mass spectrometry method for the characterization of chitosan. Nine kinds of chitosan (degree of deacetylation (DD), 63.08%–89.06% & MW, 106.1–485.0 kDa) were adopted for the method development. Most species of chitosan, detected by an ESI-MS technique, were observed below 1000 m/z , which seemed that only chito-oligosaccharide (COS) impurities were detected. Then, a sensitive UPLC-ESI-MS/MS method was established to assess the COS impurities in chitosan, and no COS impurities were detected. However, dissociation of chitosan and COSs in the ESI source were observed, and then the mass spectra patterns were deeply evaluated via an accurate Q-TOF mass spectrometer. Our research demonstrated that the mass spectra of COSs and chitosan resulted from the dissociation of glycosidic linkage and dehydration. Although the distribution of GlcN and GlcNAc units in these chitosan samples might be different, similar dissociation efficiencies were observed. Furthermore, good linearities were obtained between the intensities of product ions, detected by an UPLC-pseuedo-MS2 method, and DDs determined by conventional method. This method could be suitable for the DD determination and quantitative analysis of chitosan.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2024
·
IF 2.4
Xiaohong Yu,Hengyuan Cao,Xiaochen Liu,Jinbin Liu,Yongmei Lyu,Dujun Wang,Ming Wei
Feruloylated oligosaccharides (FOs) generated by decomposing plant hemicellulose , offer a wide range of potential applications in both the food and biomedical areas. As a graminaceous plant , bamboo is rich in hemicellulose . However, the structural composition and activity studies of FOs from it were rarely reported. In this study, FOs from Phyllostachys acuta (pFOs) obtained by enzymatic hydrolysis were isolated by AmberliteXAD-2 and C18 SPE columns. Then, pFOs were qualitatively and quantitatively analyzed by UPLC-ESI-MS/MS after labeled by 3-Amino-9-ethyl-carbazole (AEC), and the chemical antioxidant activity of pFOs and effects of pFOs on H 2 O 2 -induced oxidative damage were investigated. Finally, 14 of pFOs isomers were distinguished and identified, of which 10 did not contain hexoses and 4 did, and the three most abundant pFO structures were 12 (Iso 7, F1A1X2H2-AEC, 29.04 %), 11 (Iso 6, F1A1X1H2-AEC, 17.96 %), and 4 (Iso 3-1, F1A1X3-AEC, 15.57 %). The results of antioxidant studies showed that pFOs possessed certain reducing power, scavenging DPPH radicals, scavenging superoxide anion radicals, and scavenging hydroxyl radicals . Among them, the ability to clear DPPH radicals was particularly significant. pFOs significantly reduced the viability of RAW264.7 cells after H 2 O 2 induction, whereas pFOs had a significant protective effect (p < 0.001). pFOs increased the viability of T-AOC and SOD enzymes in oxidatively damaged cells, as well as had a significant inhibition effect on ROS elevation (p < 0.001). This study lays the foundation for the structural analysis and antioxidant activity evaluation of bamboo-derived feruloyl oligosaccharides for their application in food and pharmaceutical fields.
研究领域:
植物化学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Junjun Zhu,Han Zhang,Ningxin Jiao,Yuxin Xiao,Dawei Shi,Yong Xu
This study proposed a clean sequential process, including autohydrolysis, the hydrolysis of endoxylanase, and adsorption with activated carbon (AC), that assisted refining poplar ( Populus spp.) into low degree of polymerization (DP) xylooligosaccharides (XOS). First, autohydrolysis was conducted at 170 °C for 50 min. The autohydrolysate was then hydrolyzed with 1.0 International Unit (IU) per mL of endoxylanase to increase the concentration of low DP XOS (X2-X4) from 2.98 to 6.42 g/L. The recovery of XOS from the XOS rich enzymatic hydrolysis liquor reached 96.5% using 10% AC with a gradient elution of ethanol, and the highest purity of XOS was 98.2% from the 40% ethanol eluent. Finally, the separated spent liquor (SL) obtained after AC adsorption was assayed for bioactivity on rice ( Oryza sativa L.). Spent liquor revealed a positive dose-dependent bioactivity toward rice growth, which promoted seed germination, plant height, root length and fresh weight. These effects were attributed to an appropriate amount of acetic acid and XOS, which could be used as plant biostimulants.
研究领域:
生物化学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Tianxiang Zhu,Songlin Liu,Yu Gao,Xiongjie Sun,Haiming Hu,Zhigang Zhang,Wenwen Zhang,Lei Xiong,Hongtao Liu
Endoglucanases may not only act on β-1,4-linkages in β-glucan but also target d-glucose in glucomannan, which can expand their utility in the preparation of prebiotics. In this study, a highly efficient endoglucanase (BlGH5) from Bacillus licheniformis BJ2022 was expressed and characterized. BlGH5 exhibited the highest activity at pH 7.0 and 60 °C. It maintained over 80% activity at pH 4.0–7.0 and 30–60 °C. BlGH5 specifically cleaved β-1,4-glycosidic bonds linked to d-glucose. Site-directed mutagenesis results suggested that Arg91, Asn167, Glu168, Trp206, His228, Tyr230, Ser263, and Trp290 are key residues for its binding and catalytic activity. Moreover, BlGH5 displayed high activity against konjac glucomannan (KGM), indicating that BlGH5 could be used to prepare low-molecular-weight konjac glucomannan (KGM-L). Based on the physicochemical properties of KGM and KGM-L, KGM-L was characterized by reduced molecular weight and viscosity. Fecal fermentation experiments demonstrated that KGM and KGM-L could promote the production of short-chain fatty acids (SCFAs). Still, KGM-L was more conducive to the growth of the gut probiotics. In conclusion, we identified an endoglucanase for the degradation of KGM. Results indicated that KGM-L would have superior prebiotic effects. Thus, our study provides a basis for the future development and application of KGM-L as a prebiotic.
研究领域:
酶学
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2018
·Vol. 54
·期 1
·170-182
·
IF 2.383
Hong He,Fengping An,Hui Teng,Qun Huang,Hongbo Song
Summary This study aimed to synthesise a complex of agar oligosaccharides (AOS) with ferric iron and to evaluate its physicochemical and structural characteristics. Based on single factor, Plackett–Burman and response surface optimal experiments of three factors at three levels, the optimised chelate conditions of the AOS-iron (III) complex were mass ratio of AOS to iron ions of 4.5:1 g:g, reaction temperature of 74 °C and reaction time of 60 min. Under these conditions, the iron content and total sugar content were 14.03 ± 0.42% and 66.07 ± 0.96%, respectively. The physicochemical analysis showed that AOS–iron (III) was soluble and steady at physiological pH, and it was easily reduced to iron (II). The structural characteristics of AOS and AOS–iron (III) were analysed by Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Nuclear magnetic resonance, and results showed that the iron (III) ions were chelated to AOS by –OH groups and –COOH groups and widely dispersed on the AOS backbone. Therefore, AOS–iron (III) could be a candidate for iron supplementation to treat iron deficiency anaemia.
研究领域:
化学
·
材料科学
·
营养学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 11
·2286
·
IF 4.6
Jianshen Liu,Xinghua Guo,Qishi Che,Zhengquan Su
Although calcium phosphate bone cement has some advantages (it is easy to form, self-curing, and does not produce heat), some disadvantages remain that limit its clinical application. Therefore, the question of how we can modify CPC and further improve the various properties of calcium phosphate bone cement is a current research hotspot. In this paper, the preparation conditions and technology of biphasic calcium phosphate (BCP) were optimized; chitosan oligosaccharide (COSM) with MW ≤ 3000 Da was added to the optimal formulation of biphasic calcium phosphate cement particles, and its physical and chemical properties were characterized. The results showed that BCP bone cement carrier for clinical operations was successfully constructed by the high-temperature solid-state reaction method, and COSM-BCP bone cement particles were obtained by loading COSM drugs with an angiogenesis effect. Its formula is biphasic calcium phosphate powder with the molar ratio of α-TCP/β-TCP of 1. The curing time of the prepared BCP particles is 24 ± 1 min, the compressive strength is 29.58 ± 1.89 MPa, and the porosity reaches 52.09%. The loaded COSM can be released continuously and stably in vitro, and has the effect of promoting angiogenesis. The safety evaluation of COSM-BCP bone cement particles and the preliminary pharmacodynamic study of its angiogenesis in vitro provide a promising clinical application basis for the development of drug-loaded biological bone substitute materials.
研究领域:
生物材料学
·
骨科医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）