Low back pain (LBP) resulting from sciatic nerve compression presents major challenges in pain management, as traditional therapies provide only short-term relief and pose risks of systemic toxicity. In this study, an innovative Fe 3 O 4 @ZIF-8-RVC (FZR) dual-responsive nanoplatform is introduced that integrates magnetic targeting with pH-sensitive, sustained drug release to overcome these limitations. The FZR nanoplatform encapsulates ropivacaine (RVC) within the ZIF-8-coated Fe 3 O 4 core, enabling precise and prolonged analgesia at the injury site through magnetic guidance and acid-triggered release. In vitro and in vivo assessments indicate that FZR achieves high drug loading, sustained release in acidic environments, and excellent biocompatibility, significantly extending analgesic effects in chronic nerve injury models while minimizing systemic exposure. Behavioral tests and molecular analyses in LBP rat models confirm that FZR effectively suppresses pain-related neuronal activity and central sensitization markers. This dual-responsive nanoplatform FZR offers a safe, long-lasting, and targeted therapeutic approach, holding strong potential for advancing pain relief in LBP and related neuropathic pain conditions.
Qianwen Yue,Cai Wang,Ke Zhang,Huiyu Wan,Baoliang Sun,Jingguo Wu,Jingyi Sun,Ying Wang
Thoracolumbar fascia (TLF) plays an important role in the biomechanics of the spine. TLF often causes low back pain (LBP), leading to limb movement disorders and numbness. At present, the clinical diagnosis mainly depends on doctors' clinical experience and magnetic resonance imaging. In order to make the diagnosis more convenient and accurate, we designed a "sandwich" biosensor based on surface-enhanced Raman scattering (SERS) to detect C-reactive protein (CRP) and serum amyloid A (SAA) in fascia. In this study, Ag nanocubes (AgNCs) were used as the substrate, so that the antibodies of CRP and SAA were modified on the substrate surface. Then substrate combined with antigen to form the bottom layer of the sandwich. 4-Mercaptobenzoic acid (4-MBA) and Nairn blue chloride (NBA) were used as signalling molecule probes. Hollow gold nanoparticles (HGNPs) modified with (EDC / NHS) enabled the attachment of antibodies to CRP and SAA to the surface of HGNPs. Eventually, the complex formed by the antigen-antibody reaction reacted with the sandwich substrate after the modification reaction to form the final "sandwich" structure. We successfully applied it to the specific detection of inflammatory markers in fascial tissue of TLF. The results showed that it had high stability and repeatability, which can bring some help for clinical work.
危险品化学品经营许可证（带存储）