SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Xueqin Wang,Yan Li,Xiaoqi Li,Jianyong Yu,Salem S. Al-Deyab,Bin Ding
Determination of environmental contaminants such as trace formaldehyde mostly relies on bulky and costly analytical equipment. However, a particular demand for portable, rapid, and sensitive method that can be used in resource-limited settings has received paramount concern. Towards this need, we are presenting here a rational design of colorimetric sensor strips using electrospun cellulose nanofibrous membrane-immobilized pararosaniline. Based on the specific reaction between formaldehyde and pararosaniline, the strips could undergo a distinct color transition from light pink to purple, specifically in the presence of trace-level formaldehyde over other possible interferential volatile organic compounds. Benefiting from the large specific surface area and high porosity of nanofibrous membranes, the strips achieved ultralow naked eye detection limit of 0.06 mg m −3 (below the regulatory level of 0.1 mg m −3 ), wide linear range from 0.12 to 6 mg m −3 , and short response time (15 min) in the absence of advanced instrumentation. Additionally, the sensing responses were visualized quantitatively by HSI coordinates which are presented with lightness and chromatic values and analyzed by a principal component analysis method. Furthermore, the strips exhibited remarkably improved analytical performance compared to commercial filter paper-based ones. With its cost-effective design and portability, this sensitive and specific colorimetric strips could potentially be used for providing air contamination information.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Chen Yang,Guoqing He,Shukun Zhao,Shanpeng Dong,Yantong Liu,Yifan Wang,Tao Jiang,Yihan Ma,Longfei Chen,Xiaotong Si,Shilun Feng,Xuan Xiao,Yi Yang
Real-time detection of chloramphenicol (CAP) in food can effectively safeguard food safety. However, traditional chloramphenicol detection methods require large testing equipment, specialized operators and long testing times, therefore portable and rapid CAP detection remain a challenge. Here, we present a differential encodable microspheres separation system based (DEMSs) rapid, portable and accurate detection of CAP. DEMSs utilize specific binding of CAP-Antibody in DEMSs to antigen (CAP) in sample and filtration separation using a customized microfluidic chip for probe-free and wash-free CAP immunoassays. Integrated microscopic optical system and smart counting algorithms based on Hough Circle Transform deployed on a smartphone app enable portable and on-site CAP detection. The experimental results showed that the limit of detection (LoD) of CAP is 24.3 pg/mL and the detection range is 30-50,000 pg/mL. The single testing time is only 16 min. The device offers a novel CAP immunoassay strategy. By replacing antibodies coupled to encoded microspheres in DEMSs, this system is expected to enable the detection of multiple substances in foods.
研究领域:
食品安全
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 43
·5168-5176
·
IF 2.596
Liying Peng,Yu Guo,Ting Gu,Haiyang Li,Shan Jian,Dai Jia,Jun Sun
Biogenic dimethyl sulfide (DMS) has attracted widespread attention over several decades due to its potential role in linking ocean biology and climate. The air-to-sea exchange flux, estimated based on marine DMS concentration, offers useful information for evaluating its contribution to climate change. As such, field observation techniques with the characteristics of fast testing speed, portability and easy operation are in demand to accurately monitor the DMS in seawater. In this paper, we proposed a new strategy for the sensitive field measurement of DMS in seawater based on benzene-assisted photoionization positive ion mobility spectrometry (BAPI-PIMS) coupled with a time-resolved introduction. Benzene was employed as a dopant to improve the selectivity by keeping the other sulfur compounds from being ionized, while the two-dimensional data versus drift time and retention time were obtained via an online separating column to eliminate the adverse impact of environmental moisture. Under the optimization conditions, the LODs (S/N = 3) for two product-ion peaks (PIPs) of DMS decreased to 0.081 nmol L−1. Finally, the established method was applied to the lab and ship-board analysis of seawater from the Bohai Sea and the North Yellow Sea in the summer of 2019, and DMS in surface seawater was in the range of 0.11–23.90 nmol L−1 with an average of 9.88 ± 6.96 nmol L−1, indicating the potential for the field detection of marine DMS.
研究领域:
海洋生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Biomicrofluidics
·
2017
·Vol. 11
·期 6
·
IF 2.535
Jia Yuan,Dong Hui,Zheng Jianping,Sun Hao
Particulate matter (PM), a key indicator of air pollution by natural and anthropogenic activities, contributes to a wide spectrum of diseases that lead to a shortening of life expectancy. It has been recognized that trace metals in airborne PM are highly toxic and can be correlated with lesion in respiratory, gastrointestinal, immunological, and hematological systems. Traditional methods for trace metal assay require sophisticated instrumentations and highly trained operators in centralized laboratories. In this work, by integrating the technologies of microfluidic paper-based analytical devices, additive manufacturing, smartphone, and colorimetric sensing, we developed the first smartphone based paper microfluidic platform for portable, disposable, and quantitative measurements of cobalt (Co), copper (Cu), and iron (Fe) in ambient air and street sediments. On a single A4-sized paper, 48 devices were fabricated in under 30 s with a total cost of ∼$1.9. On each device, 12 reaction units were patterned and used for colorimetric tests. Particulate samples from urban ambient air and street sediments were collected, processed, and analyzed. Signals of the on-chip complexation product were recorded using a smartphone camera and processed by a self-developed app on an iOS system. For precisely controlling the object distance, chip position, and luminance, a hand-held 3D cellphone housing was designed and printed. The detection limits of Co, Cu, and Fe were determined to be 8.2, 45.8, and 186.0 ng, while the linear dynamic ranges were calculated to be 8.2–81.6, 45.8–4.58 × 10 2 , and 1.86 × 10 2 –1.86 × 10 3 ng, representing a practically relevant device performance with a significant reduction in the detection cost and time consumption. Trace metals in ambient air and sediments of two cities in China have been quantified portably, thus demonstrating the utility of our system in improving strategies for air pollution control in low-resource settings.
研究领域:
环境科学
·
分析化学
·
生物医学工程
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DOI
高影响因子
ANALYST
·
2019
·Vol. 145
·期 2
·537-543
·
IF 4.019
Cuilan Zhang,Xiao Ling,Qingsong Mei,Hongbo He,Shengsong Deng,Yong Zhang
The sensitive and on-site detection of sulfur dioxide (SO2) is in great demand in the fields of food safety and environmental protection. Here, we developed a novel upconversion nanoprobe based on the luminescence energy transfer mechanism for monitoring the atmospheric SO2 concentrations. The lanthanide emitters, Tm3+ ions, were optimized to be doped on the surface layer of the upconversion nanoparticles to improve their energy transfer efficiency by minimizing the distance between the emitters and the surface quencher, a cyanine dye. As a proof-of-concept, the optimal nanoprobe was utilized to detect SO2 water derivatives, bisulfite ions, exhibiting a linear luminescence increase in the range of 1 nM to 10 nM. Furthermore, we assembled the cyanine-modified upconversion nanoparticles onto a test paper, and used a smartphone-based detection platform to achieve portable and visual detection of SO2. The test paper showed a strong luminescence stability, homogeneity and good anti-interference. The limit of detection for SO2 gas was found to be 1 ng L−1. This novel upconversion test paper was also demonstrated to directly monitor the concentration of SO2 gas in atmosphere.
研究领域:
环境科学
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分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Shanglin Li,Ziyang He,Yonghong Meng,Baoxin Li,Mei Liu
Aflatoxin B1 contamination poses severe threats to food safety, vitally requiring reliable assays. Biosensors are effective but subject to unsatisfactory signal conversion efficiency owing to their usage of the monovalent probes with redundant materials and procedures. Herein, we developed a photothermal aptasensor, which combined multifunctional polyvalent DNA scaffolds with thermal imaging for the portable detection of aflatoxin B1. The aptasensor was conferred with favorable properties of specific recognition, surface modification and signal amplification by the polyvalent DNA scaffolds, thereby showed enhanced analytical performance with simplified procedures. Moreover, the detection potential of the aptasensor was further improved by introducing Cu x S nanoparticles to provide intuitive temperature output for portable devices analysis. Consequently, AFB1 could be quantified in a wide range of 1–500 ng/mL with the detection limit of 0.75 ng/mL, merely using common thermometer. For food samples, the spiked recoveries of the proposed method ranged from 93.5 % to 108.7 % with relative standard deviation from 2.9 % to 6.3 %. These results were in agreement with the high-performance liquid chromatography method, demonstrating the good accuracy of this method. Therefore, this method is expected to be extended as a universal detection strategy for food hazards analysis.
研究领域:
食品科学
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生物传感技术
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SPECTROSCOPY
·
2024
·
IF 2.2
Hang Zhao,Xinyu Cui,Panshuo Zhang,Min Zhou,Chunlin Liu,Xiaofeng Shi,Jun Ma
Current label-free surface-enhanced Raman spectroscopy (SERS) assay for the detection and analysis of organophosphorus pesticides has achieved initial success, but the application still faces constraints of substrate portability and specificity. To this end, this paper demonstrates a method for portable, rapid, and specific detection of low concentrations of fenthion pesticides based on a solid substrate of gold nanoparticle monolayers combined with molecularly imprinted polymers (MIPs). The nano-monolayers were transferred to the surface of mercapto-silicon wafers by interfacial self-assembly technique to form a stable connection with S–Au bonds and, at the same time, prevent nanoparticles from dropping off during the surfactant removal process. Then, the fenthion MIPs were directly generated on the surface of the monolayer film by spin-coating with a pre-polymerization solution and ultraviolet-induced polymerization. Tests showed that the molecular imprint was able to accurately bind to fenthion, but not other molecules, in a mixture of structural analogs, achieving a low concentration detection of 10–8 mol/L. The composite substrate maintained a signal uniformity of a relative standard deviation (RSD) = 7.05% and a batch-to-batch reproducibility of RSD = 10.40%, making it a potential pathway for the extended application of SERS technology.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Yue Li,Ping Liu,Zhongshan Guo,Wenqian Li,Haiyan Jiang,Yongchao Ma
Food safety requires both efficient removal of organic dyes and on-site visualization, yet most methods are single-function, focusing only on degradation or detection. We propose a π-conjugated porous vesicular carbon nitride (CPCN), where pyrimidine rings are introduced via hydrogen-bonding self-assembly, forming an in-plane Z-type homojunction within the g-C3N4 skeleton and enabling electron density redistribution. The vesicular hierarchical porous structure enhances light absorption and interfacial reactivity. Multiscale characterization and theoretical analysis show that the built-in electric field significantly promotes exciton dissociation and carrier migration, achieving rapid degradation of malachite green (MG) under visible light with ∼99.3% efficiency within 30 min. A ratiometric fluorescent probe using rhodamine B and CPCN nanosheets (CPCN NS) enables highly sensitive MG detection with a limit of 0.013 μg mL–1. CPCN loaded in sodium alginate gel microspheres easily removes malachite green from fish tissues, enabling portable on-site detection in conjunction with fluorescent test strips with cell phone RGB recognition. This provides a scalable solution for environmental remediation and food safety.
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Qinghua Hu,Qiuxiang Huang,Kexin Liang,Yuyuan Wang,Yu Mao,Qiang Yin,Hongqing Wang
Colorless and highly toxic phosgene has long been considered to be a great menace to human health and national security. Excellent AIE + TICT characteristic ratiometric and colorimetric fluorescent sensor TPE-CI was constructed with the combination of the TICT based 3-benzo[d]imidazole-chromen-2-imine unit and AIE based tetraphenylethene (TPE) unit. TPE-CI can detect phosgene not only in organic solvent through a change from the TICT process to the AIE process, but also in solid-state through a new change from the AIE + TICT process to the AIE process. TPE-CI exhibited convenient to use as a test strip, rapid response (less than 6 s in solvent and 2 min in air atmosphere), excellent selectivity and fair sensitivity for visual inspection of trace phosgene in the organic solvent and in the gas phase. The limit of detection (LOD) was calculated and derived as 0.36 μM in solvent and 0.27 ppm in the air atmosphere, which was far below the acute phosgene exposure level of human response. The satisfactory results indicate that this AIE + TICT strategy may offer valuable thinking to develop convenient solid-state optical sensors with dual ratiometric and colorimetric fluorescence response for gaseous phosgene identification.
研究领域:
化学传感器
·
材料科学
·
环境监测
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wei Nie,Ruoxian Zhang,Chao Hu,Tengchuan Jin,Xi Wei,Hua Cui
SARS-CoV-2 antigen detection plays a key role in the rapid diagnosis of COVID-19. However, current clinically used immunoassays are often limited by assay throughput, sensitivity, accuracy, and field operating conditions. To address these challenges, we constructed a self-enhanced electrochemiluminescence (ECL) array chip (SE 2 AC) for highly sensitive and label-free detection of SARS-CoV-2 nucleocapsid protein (N protein) with a facile and portable assay setup. Firstly, the self-enhanced ECL nanomaterials with inherent film-forming properties were synthesized by co-doping Ru(bpy) 3 2+ and polyethyleneimine (PEI) in silica nanoparticles (Ru/PEI@SiO 2 ). Secondly, a resistance-induced potential difference-based single-electrode electrochemical system (SEES) was adapted to serve as the electrode array to facilitate one-step assembly without the need for chip alignment. Thirdly, the chip electrode array was functionalized with the synthesized self-enhanced ECL emitters and captured antibodies. In addition, a portable detection box equipped with a smartphone was 3D-printed to serve as the chip holder and “dark room” for imaging acquisition. The SE 2 AC performance was validated with N protein with a limit of detection (LOD) of 0.47 pg/mL in the range of 1–10,000 pg/mL. Furthermore, the chip successfully detected the viral antigen residue as low as 1.92 pg/mL from diluted rehabilitation patients’ serum samples. This is the first study reporting label-free detection of SARS-Cov-2 N protein based on a self-enhanced ECL immunosensor, which provides a novel facile method for highly sensitive diagnosis of COVID-19 with high throughput, portability, and low cost.
研究领域:
分析化学
·
生物医学工程
·
医疗诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Tao Tian,Hao Zhang,Feng-Qing Yang
A rapid, portable, and cost-effective method using personal glucose meter (PGM) for quantitative analysis of hydrogen peroxide (H 2 O 2 ) was established based on ascorbate oxidase (AAO)-catalyzed reaction for the first time. Ascorbic acid (AA) can rapidly reduce ferricyanide (K 3 [Fe(CN) 6 ]) to ferrocyanide (K 4 [Fe(CN) 6 ]) in the glucose test strip and transfer electron to the electrode to generating a PGM detectable signal. Thus, the concentration of AA can be directly determined by the PGM as simple as measuring the blood glucose. On the other hand, AAO can catalyze the reduction of H 2 O 2 and produce an enzyme-peroxide complex, which decreases the yields of dehydroascorbic acid formed by the oxidation of AA, resulting in the increase in PGM detectable signal of residual ascorbic acid (re-AA). Therefore, the concentration of H 2 O 2 is proportional to the concentration of re-AA. After optimization of the experimental conditions, the developed method can be used to detect H 2 O 2 at linear range of 2.5–5 × 10 3 μM with the quantification limit of 2.5 μM. In addition, the satisfactory spiked recoveries (95.3–108.9 %) of real samples (i.e., tap water, contact lens solution, medical hydrogen peroxide, and normal human serum) confirm its feasibility for practical applications. In short, this study provides a feasible PGM-based method for H 2 O 2 detection with simple operations.
研究领域:
分析化学
·
生物化学
·
环境科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 14
·3219-3224
·
IF 5.227
Jing Lei,Lu Shi,Wei Liu,Baoxin Li,Yan Jin
Portable detection of cancer cells is important for early-stage diagnostic applications and prognosis of cancer. Herein, a simple and sensitive chemiluminescence method was proposed for portable detection of cancer cells via a handheld luminometer. It is based on the cancer cell triggered cyclic strand displacement reaction (SDR) for signal amplification. Cancer cells, CCRF-CEM, bind to their aptamer and release the trigger DNA (TDNA) of SDR. The TDNA initiates the cyclic SDR between magnetic bead modified hairpin DNA 1 (MB-H1) and HRP-tagged hairpin DNA 2 (HRP-H2), resulting in an enrichment of HRP onto the surface of magnetic beads by forming H1/H2 duplexes. HRP catalyzed luminol/H2O2/PIP solution to produce a strong chemiluminescence (CL) signal. CCRF-CEM cells were sensitively detected by combining magnetic enrichment with the signal amplification of SDR. The CL intensity showed an excellent linear relationship with the number of CCRF-CEM cells in the range of 100 to 5 × 104 cells per mL. The detection limit was as low as 85 cells per mL. Therefore, it offers a sensitive, cheap and portable method for the chemiluminescence detection of cancer cells and provides a new option for the early diagnosis of cancers.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）