MOLECULES
·
2023
·Vol. 28
·期 5
·2104
·
IF 4.6
Zhijin Yang,Zhiwei Xue,Kejie Zheng,Yule Zhang,Guorong Sui,Haima Yang,Songlin Zhuang,Lulu Zheng,Dawei Zhang
Undoubtedly, SARS-CoV-2 has caused an outbreak of pneumonia that evolved into a worldwide pandemic. The confusion of early symptoms of the SARS-CoV-2 infection with other respiratory virus infections made it very difficult to block its spread, leading to the expansion of the outbreak and an unreasonable demand for medical resource allocation. The traditional immunochromatographic test strip (ICTS) can detect one analyte with one sample. Herein, this study presents a novel strategy for the simultaneous rapid detection of FluB/SARS-CoV-2, including quantum dot fluorescent microspheres (QDFM) ICTS and a supporting device. The ICTS could be applied to realize simultaneous detection of FluB and SARS-CoV-2 with one test in a short time. A device supporting FluB/SARS-CoV-2 QDFM ICTS was designed and had the characteristics of being safe, portable, low-cost, relatively stable, and easy to use, ensuring the device could replace the immunofluorescence analyzer in cases where there is no need for quantification. This device does not need to be operated by professional and technical personnel and has commercial application potential.Keywords:SARS-CoV-2;FluB;immunochromatographic test strip;rapid fluorescence detection
研究领域:
医学诊断
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病毒学
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生物技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
Micromachines
·
2021
·Vol. 12
·期 10
·1182
·
IF 2.891
Wenwen Chen,Yingying Xie,Yuang Chang,Yuhai Xu,Mengqian Zhao,Pengwei Deng,Jianhua Qin,Hongjing Li
Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis.Keywords:exosome;separation;chitosan scaffold
研究领域:
生物技术
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纳米技术
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Small
·
2023
·Vol. 19
·期 28
·2301162
·
IF 13.3
Zewei Lian,Tingqing Wu,Huadong Wang,Jimei Chi,Lijun Cheng,Daixi Xie,Xiangyu Pan,Yuming Hu,Zhiyu Tan,Sisi Chen,Xu Yang,Yang Yun,Wei Wu,Chunbao Li,Meng Su,Yanlin Song
Rapid and ultra-sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for early screening and management of COVID-19. Currently, the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the primary laboratory method for diagnosing SARS-CoV-2. It is not suitable for at-home COVID-19 diagnostic test due to the long operating time, specific equipment, and professional procedures. Here an all-printed photonic crystal (PC) microarray with portable device for at-home COVID-19 rapid antigen test is reported. The fluorescence-enhanced effect of PC amplifies the fluorescence intensity of the labeled probe, achieving detection of nucleocapsid (N-) protein down to 0.03 pg mL −1 . A portable fluorescence intensity measurement instrument gives the result (negative or positive) by the color of the indicator within 5 s after inserting the reacted PC microarray test card. The N protein in inactivated virus samples (with cycle threshold values of 26.6–40.0) can be detected. The PC microarray provides a general and easy-to-use method for the timely monitoring and eventual control of the global coronavirus pandemic.
研究领域:
生物医学工程
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诊断医学
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分子生物学
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病毒学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Danting Yan,Boyu Zhu,Jingjing Wang,Mengke Wu,Fang Xie,Peipei Qi,Hongping Gong,Xingyu Lin
Furazolidone (FZD) is a banned antibiotic in food-producing animals due to its carcinogenicity and toxicity. Its illegal use and residue persistence in foods remains a health risk, highlighting the need for a rapid and reliable detection. Electrochemical sensing offers rapidity and sensitivity; however, its use is limited by bulky instrumentation and low accuracy in complex food matrices, making on-site testing difficult. To overcome these limitations, this work developed a wireless portable intelligent nanosensor for direct, pretreatment-free detection of FZD in food. The nanosensor features a mesoporous silicon film microelectrode (MSFM) with nanopores, which significantly enhances selectivity through size exclusion, electrostatic repulsion, hydrophilic interaction, and nanoscale confinement effects. Integrated with a smartphone via Bluetooth, the nanosensor allows immediate visualization of detection results. Under optimized conditions, the nanosensor can directly detect trace amounts of FZD in fish samples in real time without the need for enrichment or preprocessing. It exhibited a wide linear range from 0.1 to 100.0 μmol/L and a detection limit of 0.05034 μmol/L, with excellent anti-fouling ability, reproducibility (RSD 4.50 %), and 15-day signal stability. This innovative wireless sensing technology holds great promise for extending laboratory-based detection techniques to on-site and market environments, particularly in resource-constrained areas.
研究领域:
分析化学
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生物传感器
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食品安全
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纳米技术
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电化学
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环境监测
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2023
·
IF 12.6
Limin Yang,Heng Guo,Ting Hou,Jingang Zhang,Feng Li
Breast cancer (BC) is the most common cancer in the world and circulating tumor cells (CTCs) are reliable biomarkers for early breast cancer diagnosis in a non-invasive manner. However, effective isolation and sensitive detection of BC-CTCs by portable devices in human blood samples are extremely challenging. Herein, we proposed a highly sensitive and portable photothermal cytosensor for direct capture and quantification of BC-CTCs. To achieve efficient isolation of BC-CTCs, aptamer functionalized Fe 3 O 4 @PDA nanoprobe was facilely prepared through Ca 2+ -mediated DNA adsorption. To further detect the captured BC-CTCs with high sensitivity, multifunctional two-dimensional Ti 3 C 2 @ [email protected] nanozyme was synthesized, which not only possessed superior photothermal effect but also exhibited high peroxidase-like activity for catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to produce TMB oxide (oxTMB) with a strong photothermal characteristic, combining with Ti 3 C 2 @ [email protected] to synergistically amplify the temperature signal. Moreover, numerous Ti 3 C 2 @ [email protected] nanocomposites would be selectively attained on the BC-CTCs surface through multi-aptamer recognition and binding strategy, which further enhanced the specificity and facilitated signal amplification . Therefore, direct separation and highly sensitive detection of BC-CTCs was successfully achieved in human blood samples. More significantly, the controlled release of the captured BC-CTCs without affecting cell viability could be straightforwardly realized by a simple strand displacement reaction. Thus, with the distinct features of portability, high sensitivity, and easy operation, the current method holds great promise for early diagnosis of breast cancer.
研究领域:
生物医学工程
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癌症诊断
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纳米技术
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生物传感器
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Junning Ma,Yue Guan,Fuguo Xing,Yan Wang,Xu Li,Qiang Yu,Xiaohua Yu
To develop a sensing platform for onsite determination of AFB 1 in foodstuffs, we developed smartphone-based chemiluminescence detection of AFB 1 via labelled and label-free dual modes. The labelled mode was characteristic of double streptavidin–biotin mediated signal amplification, obtaining limit of detection (LOD) of 0.04 ng/mL in the linear range of 1–100 ng/mL. To reduce the complexity in the labelled system, a label-free mode based on both split aptamer and split DNAzyme was fabricated. A satisfactory LOD of 0.33 ng/mL was generated in the linear range of 1–100 ng/mL. Both labelled and label-free sensing systems achieved outstanding recovery rate in AFB 1 -spiked maize and peanut kernel samples. Finally, two systems were successfully integrated into smartphone-based portable device based on custom-made components and android application, achieving comparable AFB 1 detection ability to a commercial microplate reader. Our systems hold huge potential for AFB 1 onsite detection in food supply chain.
研究领域:
分析化学
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食品科学
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生物技术
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2023
·Vol. 19
·期 26
·2300464
·
IF 13.3
Jing Wu,Yong Liu,Lijun Peng,Qisijing Liu,Dawei Wang,Xu Jing,Yaozhong Hu,Jing Lin,Hanyue Fu,Xuemeng Ji,Jingmin Liu,Huan Lv,Bo Peng,Bowei Zhang,Liqiong Guo,Shuo Wang
This work reports a portable, origami-type paper device with a plasmonic fluor-labeled microneedle sensing module for the multiplexed quantification of anemia biomarkers in whole blood. Sequential steps, including serum separation, target enrichment, and multiplexed readout by a gel imager, are rapidly accomplished with the flexible and highly integrated device. The microneedle array enabled efficient sampling of trace targets from ng mL −1 to pg mL −1 level. Combined with the plasmonic fluor label, the signal is improved by ≈7.6 folds compared with the flat substrate-based assay. The device is applied to simultaneously quantify hemoglobin (Hb), ferritin, folic acid (FA), and vitamin B 12 (VB 12 ), which are four anemia biomarkers distributed in different environments with different concentration ranges. Featured by the small sample volume (150 µL), short assay time (20 min), low cost (2 $), robust stability, and user-friendliness, the device is promising for the rapid and accurate diagnosis of anemia in real practice.
研究领域:
生物医学工程
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即时诊断
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纳米技术
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医疗设备
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临床化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Information Processing in Agriculture
·
2022
·
IF 0
Xianbao Xu,Zhuangzhuang Bai,Tan Wang
Portable measurement of ammonia nitrogen in water is of great significance for water quality monitoring. It’s beneficial to reduce biological diseases and promote aquatic product safety. Traditional methods such as Nessler’s reagent method suffer from complex operation, time delays and toxic residues. To realize simple and pollution-free detection, this paper develops a low-cost portable device for ammonia nitrogen detection. A test paper was proposed to cooperate the device and offer a chromogenic reaction. The portable device reduces the impact of any ambient light, simplifies the operation, and provides human–computer interaction. The result obtained for the detection range of 0.4–10 mg/L (R 2 are 0.990 2 and 0.989 3 for the rang of 0.4–4.5 and 4.5–10 mg/L, respectively) with the detection limit of 0.36 mg/L, and the average recovery of aquaculture water is 100.98–137.75%. The results show that the portable device can provide a great potential for on-site detection ammonia nitrogen concentration.
研究领域:
环境科学
·
水质分析
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分析化学
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水产养殖
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DOI
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Liu-Na Wei,Lin Luo,Hong-Tao Lei,Tian Guan,Cheng Jiang,Qing-Chun Yin,Zhen-Lin Xu,Chenzhong Li
Investigating organic carriers’ utilization efficiency and bioactivity within organic–inorganic hybrid nanoflowers is critical to constructing sensitive immunosensors. Nevertheless, the sensitivity of immunosensors is interactively regulated by different classes of biomolecules such as antibodies and enzymes. In this work, we introduced a new alkaline phosphatase–antibody–CaHPO4 hybrid nanoflowers (AAHNFs) microreactor based colorimetric immunoprobe. This system integrates a biometric unit (antibody) with a signal amplification element (enzyme) through the biomineralization process. Specifically, the critical factors affecting antibody recognition activity in the formation mechanism of AAHNFs are investigated. The designed AAHNFs retain antibody recognition ability with enhanced protection for encapsulated proteins against high temperature, organic solvents, and long-term storage, facilitating the selective construction of lock structures against antigens. Additionally, a colorimetric immunosensor based on AAHNFs was developed. After ascorbic acid 2-phosphate hydrolysis by alkaline phosphatase (ALP), the generated ascorbic acid decomposes I2 to I–, inducing the localized surface plasmon resonance in the silver nanoplate, which is effectively tuned through shape conversion to develop the sensor. Further, a 3D-printed portable device is fabricated, integrated with a smartphone sensing platform, and applied to the data of collection and analysis. Notably, the immunosensor exhibits improved analytical performance with a 0.1–6.25 ng·mL–1 detection range and a 0.06 ng·mL–1 detection limit for quantitative saxitoxin (STX) analysis. The average recoveries of STX in real samples ranged from 85.9% to 105.9%. This study presents a more in-depth investigation of the recognition element performance, providing insights for improved antibody performance in practical applications.
研究领域:
生物化学
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生物技术
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分析化学
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纳米技术
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 26
·7731-7740
·
IF 4.478
Liao Jun,Yu Zhicheng,Fu Li,Liu Jia,Jia Li
Realizing the rapid and on-site detection of biothiols in complex biological and food samples using simple assays and devices remains a major challenge. In this study, biothiols containing sulfhydryl groups were found to be able to inhibit the photo-triggered oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Based on the discovery, using the commercially available and low-cost TMB as the chromogenic substrate, an enzyme-free colorimetric approach was developed for the rapid determination of biothiols. The method does not involve the introduction of any natural enzymes, nanoenzymes, and external oxidants. The mechanisms of the photoinduced oxidation of TMB and the detection of biothiols were proposed. Furthermore, a smartphone-based portable device integrated with test strips was constructed by the 3D printing technique. This device can simultaneously meet the requirements of the photocatalytic oxidation reaction of TMB and the detection of biothiols. The entire process only takes less than 5 min. The successful detection of cysteine in urine and milk samples demonstrates the great potential of the device in the on-site assays. Graphical abstract
研究领域:
分析化学
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生物技术
·
现场即时检测
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ting Zhao,Xiaochen Liang,Xinjie Guo,Xingjie Yang,Jialiang Guo,Xia Zhou,Xueqin Huang,Wenqian Zhang,Yiqing Wang,Zhaowei Liu,Zhengjin Jiang,Hongke Zhou,Haibo Zhou
A simple, sensitive method for pesticide distinguishment based on a colorimetric sensor array using diverse gold nanoparticles (AuNPs) at room temperature is presented in this study. Acetylcholinesterase (AChE) hydrolysis ability was influenced by different pesticides and produced different concentrations of thiocholine by hydrolyzing acetylthiocholine iodide (ATCh). Thiocholine could be easily linked to the AuNPs through an Au − S covalent bond, and AuNPs underwent aggregation, resulting in a visible color change due to alteration of surface plasmon resonance properties. Based on these results, we successfully distinguished eight pesticides (glyphosate, thiram, imidacloprid, tribenuron methyl, nicosulfuron, thifensulfuron methyl, dichlorprop, and fenoprop) utilizing five different AuNPs by colorimetric assay. The limit of detection (LOD) of this visual method for all pesticides was less than 1.5 × 10 -7 M, which was more sensitive than the U.S. Environmental Protection Agency regulations specify (1.18 ∼ 3.91 × 10 -6 M). This method was further improved by combining a portable smartphone device with a color picking application using (color name AR) and RGB (red, green, blue) values. The method was successfully applied to pesticide residue distinguishment in real samples by linear discriminant analysis (LDA).
研究领域:
分析化学
·
环境科学
·
纳米技术
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Lian Xiao,Hu Di,Zhang Ruilong,Niu Helin
Indoxyl sulfate (IS), a protein-bound uremic toxin implicated in chronic kidney disease (CKD) progression and extrarenal pathologies, necessitates the development of accessible monitoring tools for point-of-care diagnostics. To address this challenge, we engineered a portable fluorescent capsule device by encapsulating an antenna-effect-enhanced europium metal–organic gel (EuMOG) within a biocompatible alginate matrix. This innovative sensor exhibits specific ratiometric fluorescence modulation toward IS via a photoinduced electron transfer (PET) mechanism, wherein IS binding triggers a distinct emission shift: quenching of Eu3+ red emission (619 nm) with concurrent 29-fold enhancement of ligand-centered blue emission (393 nm). Significantly, the capsule achieves ultrasensitive detection with record-low detection limits of 1.9 ppb in urine and 43.61 ppm in serum─surpassing conventional methods by orders of magnitude─while maintaining selectivity against structurally similar metabolites (e.g., uric acid, creatinine) and physiological interferents in complex matrices. The visually discernible pink-to-blue transition under UV light enables instrument-free, real-time semiquantification within 16 min, as validated by linear ratiometric responses across clinically relevant IS concentrations (1–900 μM, R2 = 0.996). The integration of room-temperature synthesis, dynamic capsule dissolution kinetics, and PET-driven specificity establishes a paradigm shift toward deployable CKD diagnostics, particularly for resource-limited settings.
研究领域:
生物医学工程
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分析化学
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医学诊断
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材料科学
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生物化学
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肾病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）