Pharmaceuticals
·
2024
·Vol. 17
·期 10
·1257
·
IF 4.3
Tibor Maliar,Marcela Blažková,Jaroslav Polák,Mária Maliarová,Eva Ürgeová,Jana Viskupičová
Background: The balance between antioxidants and pro-oxidants plays a significant role in the context of oxidative stress, influenced by both physiological and non-physiological factors. Objectives: In this study, 18 prescribed antibiotics (including doxycycline hydrochloride, tigecycline, rifampicin, tebipenem, cefuroxime, cefixime, potassium clavulanate, colistin, ampicillin, amoxicillin, amikacin, nalidixic acid, azithromycin, pipemidic acid trihydrate, pivmecillinam, aztreonam, fosfomycin sodium, and ciprofloxacin) were subjected to simultaneous determination of antioxidant and pro-oxidant potential to assess if pro-oxidant activity is a dominant co-mechanism of antibacterial activity or if any antibiotic exhibits a balanced effect. Methods: This study presents a recently developed approach for the simultaneous assessment of antioxidant and pro-oxidant potential on a single microplate in situ, applied to prescribed antibiotics. Results: Ten antibiotics from eighteen showed lower antioxidant or pro-oxidant potential, while five exhibited only mild potential with DPPH50 values over 0.5 mM. The pro-oxidant antioxidant balance index (PABI) was also calculated to determine whether antioxidant or pro-oxidant activity was dominant for each antibiotic. Surprisingly, three antibiotics—doxycycline hydrochloride, tigecycline, and rifampicin—showed significant measures of both antioxidant and pro-oxidant activities. Especially notable was tebipenem, a broad-spectrum, orally administered carbapenem, showed a positive PABI index ratio, indicating a dominant antioxidant over pro-oxidant effect. Conclusions: These findings could be significant for both therapy, where the antibacterial effect is enhanced by radical scavenging activity, and biotechnology, where substantial pro-oxidant activity might limit microbial viability in cultures and consequently affect yield.
研究领域:
药理学
·
微生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 2
·92
·
IF 5.118
Yue Sui,Yue Gu,Yujing Lu,Chenxu Yu,Jie Zheng,Hang Qi
Fucoxanthin (FX), a natural carotenoid found in seaweed with multiple functional activities, is unstable with a poor water solubility that limits its utilization. This study aimed to improve FX’s stability and bioavailability via the nano-encapsulation of FX in polyvinylpyrrolidone (PVP)-coated[email protected]nanoparticles (NPs). The[email protected]NPs were evaluated in terms of their morphology, stability, encapsulation efficiency (EE), loading capacity (LC), and in vitro release to optimize the encapsulation parameters, and a 1:8 FX:PVP ratio was found to perform the best with the highest EE (85.50 ± 0.19%) and LC (10.68 ± 0.15%) and improved FX stability. In addition, the[email protected]NPs were shown to effectively deliver FX into Caco-2 cancer cells, and the accumulation of FX in these cancer cells showed pro-oxidative activities to ameliorate H2O2-induced damage and cell death. The[email protected]NPs could potentially become a new therapeutical approach for targeted cancer treatment.Keywords:fucoxanthin;nanoparticles;Caco-2 cells;pro-oxidation;targeted cell death
研究领域:
药学
·
癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 13
·2504
·
IF 5.2
Qiaoyu Liu,Menglin Lei,Wenhong Zhao,Xiangluan Li,Xiaofang Zeng,Weidong Bai
In this study, lipoxygenase (LOX) extracted from dry-cured mackerel was purified, resulting in a 4.1-fold purification factor with a specific activity of 493.60 U/min·g. LOX enzymatic properties were assessed, referring to its optimal storage time (1–2 days), temperature (30 °C), and pH value (7.0). The autoxidation and LOX-induced oxidation of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:2n9c), linoleic acid (C18:2n6c), arachidonic acid (C20:4), EPA (C20:5), and DHA (C22:6n3) were simulated to explore the main metabolic pathways of key flavors in dry-cured mackerel. The results showed that the highest LOX activity was observed when arachidonic acid was used as a substrate. Aldehydes obtained from LOX-treated C18:1n9c and C18:2n6c oxidation, which are important precursors of flavors, were the most abundant. The key flavors in dry-cured mackerel were found in the oxidative products of C16:0, C18:0, C18:1n9c, C18:2n6c, and C20:4. Heptanaldehyde could be produced from autoxidation or LOX-induced oxidation of C18:0 and C18:1n9c, while nonal could be produced from C18:1n9c and C18:2n6c oxidation. Metabolic pathway analysis revealed that C18:1n9c, C18:2n6c, EPA, and DHA made great contributions to the overall flavor of dry-cured mackerel. This study may provide a relevant theoretical basis for the scientific control of the overall taste and flavor of dry-cured mackerel and further standardize its production.Keywords:lipoxygenase;fatty acids;autoxidation;enzymatic oxidation;volatile compounds
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jingwen Dong,Xueli Li,Yaoqing Zhou,Yunhao Lu,Yuanping Lv,Yuanlong Chi,Qiang He
The effect of gallic acid (GA) on the redox state of hemoglobin (Hb) and the structural mechanism upon the Hb–GA interaction were investigated. Results indicated that GA exhibited antioxidant and pro-oxidant effects on Hb, which depended on its concentration and the redox state of Hb. The antioxidant capacity of GA contributed to the inhibition of free iron release from Hb. GA could bind to the central cavity of Hb and interacted with the heme moiety through direct hydrophobic contacts as indicated by docking analysis, but GA did not disrupt the heme structure. Conversely, GA increased the compactness of the Hb molecule and might narrow the crevice around the heme pocket, which contributed to the inhibition of Hb autoxidation and the free iron release. Results provided significant insights into the interaction of GA with redox-active Hb, which is beneficial to the application of GA in relative meat and blood products.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 19
·2709
·
IF 4.7
Bochu Du,Chenghao Lee,Ying Ji
Enhancing the degradability of polyethylene plastics could provide a potential solution to the overwhelming crisis of plastic waste. Conventional studies have focused on the degradation of polyethylene thin films. This study investigated UV-induced photo-degradation according to ASTM D5208-14 in polyethylene sheets with thicknesses ranging from 0.4 to 1.2 mm. The impacts of sample thickness, metal pro-oxidants, polyethylene resin types and foaming were explored through the characterization of the carbonyl index, molecular weight, tensile properties and crystallinity. As pro-oxidants, single iron or manganese stearate demonstrated a concentration-dependent trend in accelerating the photo-degradation of polyethylene sheets. The thickness, foaming and resin type—such as low-density polyethylene (LDPE) and high-density polyethylene (HDPE)—significantly impacted the rate of photo-oxidation. Thick polyethylene sheets (1.2 mm) exhibited a heterogenous and depth-dependent degradation profile. As the photo-degradation progressed, the enhanced crystallinity, reduced UV transmittance and formation of crosslinks were able to prevent further oxidative cleavage of the polyethylene chain. This study investigated the time course and factors affecting the photo-degradation of polyethylene sheets, which could provide insights into the formulation design of photo-degradable polyethylene plastics.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Rongrong Wang,Xirong Huang
The hydrophobic ionic liquid [C8mim][PF6] (1-octyl-3-methylimidazolium hexafluorophosphate)-based bicontinuous microemulsion stabilized by the anionic surfactant [C4mim][AOT] (1-butyl-3-methylimidazolium bis(2-ethylhexyl) sulfosuccinate) was first tried as a medium for horseradish peroxidase (HRP)-triggered oxidative polymerization of aniline. The effects of the mass ratio of [C8mim][PF6]-to-water (α), the mass fraction of [C4mim][AOT] in the total mixture (γ), and temperature (T) on the enzymatic polymerization were investigated using UV–vis–NIR absorption, electron spin resonance, and small-angle X-ray scattering spectroscopy techniques. The bicontinuous microemulsion is demonstrated to play a template role in the biosynthesis of polyaniline (PANI). The conductivity of the resulting PANI depends on the microemulsion microstructure and the microstructure- and T-dependent catalytic properties of the solubilized HRP. With the increase in α, the conductivity of the synthesized PANI decreases due to the increase in the template curvature (decrease of the microdomain size) and the decrease in the activity and stability of HRP. Compared with α, γ has little effect on the microdomain size of the template; so, the γ-dependent change in the conductivity of PANI is mainly caused by the changes of the microstructure-dependent activity and stability of HRP. Over the range of 20–35 °C, T has little effect on the microdomain size, but it greatly changes the activity and stability of HRP. With the increase in T, the activity of HRP increases steadily, but its stability decreases significantly, which should be one of the reasons why the conductivity of PANI decreases with increasing T. In conclusion, lower values of α, γ, and T are favorable for the biosynthesis of conductive PANI. The present study not only deepens the insight into the role of the template in the process of PANI synthesis, but also opens up a green new way for the biosynthesis of the conducting polymer.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Li Tian,Shulin Zhang,Jianhua Yi,Zhenbao Zhu,Leqi Cui,Eric Andrew Decker,David Julian McClements
We studied the impacts of protein location (interface or aqueous phase) on the antioxidant and prooxidant activities of tea polyphenols (TP) in model oil-in-water emulsions (pH 7) at a low (0.01% w/v) or high (0.04 % w/v) concentration. TP at 0.01% reduced the levels of both lipid and protein oxidation markers in emulsions, independent of the protein location. However, TP were more potent when proteins were located at the interface. At 0.04%, TP were only weakly antioxidant towards lipids but were prooxidant towards proteins in emulsions with proteins at the interface, whereas they were still somewhat antioxidant for aqueous phase proteins. These results indicate that TP may act as either antioxidants or prooxidants depending on their concentration and also on the location of the proteins in emulsions. The level of TP should be optimized for emulsion-based foods or beverages to achieve optimum antioxidant activity.
研究领域:
食品科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yuhe Shen,Dongshuang Jia,Yuefei Wang,Tao Yu,Xiaojian Xu,Heng Chang,Qing Li,Rongxin Su,Wei Qi
Most natural biomolecules have two enantiomeric forms and can exist in either of them. Despite the L -type homochirality of natural biopolymers composed of amino acids, a great deal of effort has been devoted to the systems in which chirality dictates molecular self-assembly and corresponding properties. Previously, we demonstrated that chemical pre-modification of tyrosine ( L -Tyr) inspired by natural eumelanin can obtain colorful pigments and surface coatings , but their characteristics like stability still have development space. Here, the racemic reaction systems of tyrosine derivatives ( L/D -Tyr, Boc- L/D -Tyr and Cbz- L/D -Tyr) were designed, and we proved that substrate molecular chirality can affect the reaction rate of the oxidation process , change the intermolecular force and arrangement during assembly, thus influencing the morphology, structures, and properties of the biomimetic pigment materials. At the same time, we found that L + D -tyrosine derivatives can be oxidized and deposited on hair surfaces to obtain the color effect of black and red-brown series, and achieve green-safe biological hair dyeing. Compared with previous studies, it has faster coloring speed, higher color saturation, stronger color fixation stability, good mechanical strength and biocompatibility, providing a new idea for the manufacture of chiral-inspired advanced materials with bio-nanotechnology applications.
研究领域:
生物化学
·
材料科学
·
纳米技术
·
化妆品化学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Feilong Wei,Jianyin Miao,Huiyou Tan,Runqing Feng,Qianwang Zheng,Yong Cao,Yaqi Lan
In this study, the influence of crystal morphology and cooling temperature on the oxidative stability of oleogel-structured perilla oil emulsion was explored. The formation and distribution of the internal crystals were confirmed by differential scanning calorimetry and polarized light microscopy. All beeswax (BW) crystals existed in the internal phase while stearic acid (SA) crystals appeared in the aqueous phase as well. As the crystal grew, the HPMC/SDS emulsified layers were pierced, leading to release of the SA crystals into the aqueous phase with partial droplet flocculation. As a result, the physical and oxidative stability of the BW-structured emulsion was significantly better than that of SA-structured emulsion. The crystallinity of the emulsion increased with higher gelator concentration. The denser crystal network were more effective preventing the penetration of pro-oxidants into the internal phase, resulting in the stronger the oxidation stability. Processing methods with different cooling temperature affected more of the properties of bulk oleogels rather than the oleogel-structured emulsion for long-term storage. Overall, choosing a gelling agent that forms relatively small crystals and preparing an oleogel-structured emulsion with appropriate crystallinity can effectively improve the oxidative stability of structured emulsion with longer shelf life.
研究领域:
食品科学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 23
·13769-13779
·
IF 3.361
Yao Lu,Yi Xu,Meng-Ting Song,Ling-Ling Qian,Xiao-Lin Liu,Rong-Yao Gao,Rui-Min Han,Leif H. Skibsted,Jian-Ping Zhang
Tyrosinase, widely distributed in nature, is a copper-containing polyphenol oxidase involved in the formation of melanin. Flavonoids are most often considered as tyrosinase inhibitors but have also been confirmed to be tyrosinase substrates. Four structure-related flavonoids including flavones (apigenin and luteolin) and flavonols (kaempferol and quercetin) are found to promote not inhibit browning induced by tyrosinase catalyzed oxidation both in model systems and in mushrooms under aerobic conditions. A comparison with enzymatic oxidation and autooxidation of flavonoids alone has helped to clarify why flavonoids function as a substrate rather than an inhibitor. Flavonoids almost do not affect the kinetics of melanin formation from enzymatic oxidation of L-dopa in excess. In addition, a new brown complex formed during the reaction of flavonoid quinone and dopaquinone is suggested to enhance the browning effects by competing with isomerization and autooxidation. Structure–activity relationships of the four flavonoids in melanin formation leading to browning induced by autooxidation and enzymatic oxidation confirm the enzymatic nature of the browning.
研究领域:
生物化学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yu Liu,Qiu-Yue Cheng,Hang Gao,Hong-Yuan Chen,Jing-Juan Xu
A microfluidic gradient array is a widely used screening and analysis device, which has characteristics of high efficiency, high automation, and low consumption. Bipolar electrode electrochemiluminescence (BPE-ECL) has special value in microfluidic array chips. The combination of the microfluidic gradient and BPE arrays has potential for high-throughput screening. In this article, a microfluidic BPE array chip for gradient culture and conditional screening of cancer cells was designed. The generation of concentration gradients, continuous culture of cancer cells with high throughput, and drug screening through BPE-ECL of the Ru(bpy)32+/TPrA system can be performed in one chip. We tested gradient pro-oxidation of MCF-7 by ascorbic acid and the synergistic effect of pro-oxidation on doxorubicin. The method achieves high analysis efficiency through a BPE array while simplifying the tedious procedures required by cell culture methods.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Jun Zhao,Hanhan Wang,Hongqi Geng,Qi Yang,Yuping Tong,Weiwei He
Disturbances in the redox state of a biological system can cause diverse effects and clinical functions. The key and difficulty lies in controlling the balance between reduction and oxidation. Nanomaterials with oxidoreductase-like activity offer an appealing way to adjust anti- and pro-oxidation in response to biological conditions. To this end, we design and prepare visible light-responsive hybrid nanozymes by combining Au and N-doped carbon dots (Au/NC). It is found that Au/NC can efficiently scavenge reactive oxygen species (ROS) and free radicals and exhibit SOD-like activity, showing intrinsic antioxidant activity. Upon irradiation with visible light, we observe that Au/NC can generate ROS (e.g., hydroxyl radicals, superoxide, and singlet oxygen) and exhibit enhanced oxidase-like activity to accelerate the oxidation of chromogenic substrates, showing pro-oxidant capability. The light-activated oxidative activity is ascribed to the synergistic effects of photoexcitation of N-doped carbon dots and the surface plasmon resonance of Au nanoparticles. In addition, we demonstrate the application of the anti- and pro-oxidant capability of Au/NC in inhibition or acceleration of oxidation of Monascus pigments and Fe2+, which is switched by the incident light. These results will be of significant interest in modulating the biological redox conditions through responsive and precise nanozymes.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）