Food Science & Nutrition
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2021
·Vol. 9
·期 4
·2191-2202
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IF 2.863
Zefeng Chen,Shupeng Cheng,Huiqiong Lin,Wenjing Wu,Liyi Liang,Xicai Chen,Xi Zheng,Yan He,Kun Zhang
Acanthopanax trifoliatus (L.) Merr (AT) is a medicinal and edible plant with high nutritional value. The biological activity of A. trifoliatus (L.) Merr and its basis for injury treatment are explored in this paper. AT was ethanol-extracted then refined separately with petroleum ether, chloroform, ethyl acetate, and n-butanol. Active ingredients were analyzed, and anti-bacterial, anti-inflammatory, analgesic, and hemostatic effects were explored. Petroleum ether layer (PEL) extract produced the strongest antibacterial effect. Ethyl acetate layer (EAL) extract had the highest active substance content, with strong hemostatic and analgesic activities. Chloroform layer (CL) extract had the strongest anti-inflammatory effect and significantly reduced IL-1β protein expression. Active ingredients were analyzed using HPLC and UPLC-MS to determine saponin, polyphenol, flavonoid, and characteristic ingredient contents. EAL extract had the highest polyphenol and flavonoid levels, including rutin, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C, which may contribute to its nutritional activities. The study provides a reliable theoretical and practical basis for the applications of AT nutraceutical products.
研究领域:
药理学
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植物学
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营养科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yiwen Guo,Zitao Nie,Minjie Cao,Tian Yang,Guanjun Tao,Lijun Song,Ruijie Liu,Ming Chang,Xingguo Wang
Chimonanthus praecox (L.) Link kernel oil (LMO) has the potential to expand the variety of nutraceutical plant oils available and provide support for the application of functional food. This study aimed to assess the edible potential of LMO by examining its physicochemical characteristics, digestion behaviors, and nutraceutical properties. The results revealed that LMO has a high oil content of 40.84% and is particularly rich in linoleic acid (53.37–56.30%), oleic acid (22.04–25.08%) and triacylglycerol (TAG) of linoleic acid -palmitoleic acid- oleic acid (10.57–12.70%). The quality characteristics and phytochemical composition of LMO were found to be influenced by variety and extraction methods used. In simulated in vitro digestion tests, LMO showed a better lipid release rate and degree. Animal studies further demonstrated that LMO led to better TAG and cholesterol excretion compared to soybean oil and camellia oleifera oil. Overall, this study highlights the potential of LMO as a high-quality edible oil .
研究领域:
食品科学
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植物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2023
·
IF 5.1
Zhenyu Zhang,Li Wang,Yinqiang Wu,Chenshuo Li,Pengcheng Fu,Jing Liu
Microalgae , particularly Chlorella , have promising selenium (Se) enrichment capacity and great potential in wastewater treatment and the development of nutraceuticals. To acquire Chlorella strains with strong heterotrophic propensity and robust Se tolerance, this research utilized a heterotrophic isolation strategy along with a high Se concentration medium as selection factors. The combined sequence of the ribosomal small subunit (SSU) and internal transcribed spacer (ITS) was utilized for phylogenetic analysis , and comparative analysis of ITS secondary structures was also integrated to identify the desired strain. A novel subtropical microalgal strain, Chlorella vulgaris HNUFU001 with high Se tolerance, was isolated from water samples in a lake at Hainan University, China. Gaussian process regression was utilized to assess the strain's growth under varying Se concentrations. The intracellular accumulation and extracellular removal of Se were measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) and Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES), respectively. The maximum intracellular organic Se (92 % of total Se) was observed as 108.835 μg/g dry weight (DW) for 1 mg/L Se treatment. The highest Se removal rate was 40 %. The high Se tolerance and enrichment traits enable Chlorella vulgaris HNUFU001 to be a promising candidate for the heterotrophic production of organic Se-related nutraceuticals.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Li-Ping Peng,Chuan-He Tang
This work reports a kind of novel antioxidant Pickering high internal phase emulsions (HIPEs; with an oil fraction, ϕ > 0.74) stabilized by soy β -conglycinin ( β -CG) and polyphenol complex nanoparticles, as outstanding protective containers for lipophilic nutraceuticals. The nanoparticles with a representative polyphenol ((-)-epigallocatechin-3-gallate; EGCG) encapsulated were fabricated through an ethanol-mediated dissociation and re-assembly process of β -CG, with greater particle size and higher surface hydrophilicity observed at higher initial EGCG concentrations. Using these co-assembled nanoparticles as sole stabilizers, a kind of HIPE gels with similar gel stiffness and microstructure, could be easily fabricated at ϕ = 0.8 and a protein concentration in the aqueous phase of 1.0 wt% using polyunsatuated fatty acid-rich flaxseed oil as the dispersed phase. These HIPE gels were extraordinarily stable against heating or long-term storage, but susceptible to freeze-thawing. The as-fabricated HIPEs showed an excellent protection to β -carotene (encapsulated in oil phase) against heating, as well as an inhibition of lipid oxidation. The oxidation protection was in an EGCG concentration dependent manner. The results would be of interest for providing a novel strategy to fabricate functionalized Pickering HIPEs as potential containers or delivery systems for lipophilic nutraceuticals.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Biomed Research International
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2020
·
IF 2.276
Kiren Mustafa,Hassan Mohamed,Aabid Manzoor Shah,Shaoxuan Yu,Muhammad Akhlaq,Haifang Xiao,Shaoqi Li,Tahira Naz,Shaista Nosheen,Xueyuan Bai,Yuanda Song
Human liver cancer has emerged as a serious health concern in the world, associated with poorly available therapies. The Berberis genus contains vital medicinal plants with miraculous healing properties and a wide range of bioactivities. In this study, different crude extracts of B. lycium Royle were prepared and screened against Human Hepatocarcinoma (HepG2) cell lines. The water/ethanolic extract of B. lycium Royle (BLE) exhibited significant antiproliferative activity against the HepG2 cancer cell line with an IC50 value of 47 μg/mL. The extract decreased the clonogenic potential of HepG2 cells in a dose-dependent manner. It induced apoptotic cell death in HepG2 cells that were confirmed by cytometric analysis and microscopic examination of cellular morphology through DAPI-stained cells. Biochemical evidence of apoptosis came from elevating the intracellular ROS level that was accompanied by the loss of mitochondrial membrane potential. The mechanism of apoptosis was further confirmed by gene expression analysis using RT-qPCR that revealed the decline in Bcl-2 independent of p53 mRNA and a rise in CDK1 while downregulating CDK5, CDK9, and CDK10 mRNA levels at 48 h of BLE treatment. The most active fraction was subjected to HPLC which indicated the presence of berberine (48 μg/mL) and benzoic acid (15.8 μg/mL) as major compounds in BLE and a trace amount of luteolin, rutin, and gallic acid. Our study highlighted the importance of the most active BLE extract as an excellent source of nutraceuticals against Human Hepatocarcinoma that can serve as an herbal natural cure against liver cancer.
研究领域:
药理学
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肿瘤学
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植物学
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分子生物学
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遗传学
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DOI
PubMed
高影响因子
Sustainable Chemistry and Pharmacy
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2024
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IF 5.5
Jiao Xie,Huiqun Wang,Tingyuan Ren,Qijian Zheng,Mengxue Chen
Due to the low purity of natural ellagic acid, its clinical application has been less reported. Currently, blueberry pomace is discarded as processing waste. However, it is rich in ellagic acid and a good raw material for producing high-purity ellagic acid. Therefore, this study aimed to investigate an optimal horizontal combination scheme for producing of high-purity ellagic acid by antisolvent precipitation using crude ellagic acid extracted from blueberry pomace. Solvent screening results indicated that n-methylpyrrolidone had the highest ellagic acid solubility. Single-factor analysis showed that the oscillation speed, reaction time, crystallization temperature and antisolvent-to-solvent ratio had significantly affected ellagic acid purity, but solid-liquid ratio had little effect. A Box-Behnken-Centre combined design was used to determine the optimal combined scheme. The results showed that at a solvent concentration of 20 mg/mL, the optimal parameters were crystallization temperature 21.1 °C, reaction time 11.2 min, volume ratio of 15.6:1 antisolvent to solvent and oscillation speed of 144 r/min. Under these conditions, ellagic acid purity was 96.63%, relative standard deviation (RSD) was 1.20%, and recovery rate was 69.60%. Fourier transform infrared analysis showed that the spectral peak of purified ellagic acid matched that of the standard. In summary, the antisolvent precipitation method greatly improved the purity of ellagic acid extracted from blueberry pomace, which is not only conducive to the resource recycling, but also provides a theoretical basis for the production of high-purity ellagic acid and the possibility of expanding the application scope of ellagic acid in pharmaceuticals and nutraceuticals.
研究领域:
化学工程
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药学
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食品科学
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环境科学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 21
·12535-12540
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IF 3.269
Zong-yuan Wu,Xiao-yan Li,Min Liu,Fang Wang,Li Liu,Xue-cai Tan,Fu-hou Lei
Core–shell magnetic polymers were prepared using acrylic acid (AA) as the functional monomer, ethyleneglycol dimethacrylate (EGDMA) as the cross-linking agent, and Fe3O4@GO@SiO2–OH nano particles as the magnetic core. The core–shell materials were investigated by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FT-IR), vibrating sample strong magnetic analysis (VSM), thermogravimetric analysis (TGA) and re-binding experiments. The results showed that the core–shell materials exhibit a fast adsorption equilibrium and good adsorption capacity. The core–shell materials could be applied for the enrichment and determination of glibenclamide in hypoglycemic health products, with the recoveries of spiked samples ranging from 80.02% to 87.28%, and the RSD being ≤1.97%. The durability test shows that the core–shell materials could be reused more than 3 times through re-binding experiments. On using an external magnetic field, the absorbed analytes were efficiently separated, so it is a promising method to conveniently detect the illegal addition of glibenclamide to hypoglycemic health products.
研究领域:
分析化学
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材料科学
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药物分析
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产品关联
DOI
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
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2022
·
IF 6.157
Shuai Quan,Kaixiang Ji,Fangshuo Liu,Tesfaye Hailemariam Barkae,Mohamed Ibrahim Halawa,Saima Hanif,Baohua Lou,Jianping Li,Guobao Xu
Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10–1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
研究领域:
分析化学
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药物分析
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产品关联
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2024
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IF 4.1
Hanwei Zheng,Zhiqing Liu,Hao Zheng,Yuman Miao,Chenyu Liu,Minhua Zong,Wenyong Lou
To enhance the activity of Lactiplantibacillus plantarum FS4722 ( L. plantarum FS4722 ) in reducing uric acid, this study optimized the culture medium components and fermentation conditions using response surface methodology. The results indicated that the optimal culture medium comprised glucose at 25.61 g/L, yeast extract powder ( YEP ) at 26.86 g/L, and nucleoside addition at 0.48 g/L. The optimal fermentation conditions were: a fermentation time of 10 h, an initial pH of 6.5, a fermentation temperature of 33 °C, and an inoculum size of 1.0 %. Under these conditions, the nucleoside degradation ability of L. plantarum FS4722 increased by 41.9 times, enhancing its potential applications in reducing uric acid and developing anti-gout foods, health supplements, and pharmaceuticals.
研究领域:
微生物学
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生物技术
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食品科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Fangwei Yang,Cheng Wang,Hang Yu,Yahui Guo,Yuliang Cheng,Weirong Yao,Yunfei Xie
The risk of the illegal addition of anti-inflammatory and analgesic chemical drugs in anti-rheumatic health foods should not be ignored. Market supervision and rapid on-site detection technology need to be strengthened. Thin-layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS), which has the advantages of simple operation, fast separation, and qualitative and quantitative detection, was used in this study. And these eleven chemical drugs (acetaminophen, acetylsalicylic acid, aminophenazone, dexamethasone , diclofenac sodium , hydrocortisone, indometacin, naproxen, phenylbutazone, piroxicam , prednisone 21-acetate) that may be added to anti-rheumatic health foods have been simultaneously identified by TLC-SERS combined with chemometrics method. The characteristic signals of the separated drug spots were collected by SERS, which were optimized by the gold colloidal nanoparticles' volume and integration time. Then SERS was subjected to principal component analysis (PCA) to reduce dimensionality and combined with the pattern recognition methods in chemometrics, such as PCA-Linear Discriminant Analysis (LDA), PCA-K Nearest Neighbor and PCA-Support Vector Machine, and eleven drug components were judged and identified. Moreover, the predictive performances of different models were also analyzed and compared. The results showed that the TLC plate and four organic solvents of petroleum ether, chloroform , ethyl acetate and acetic acid were selected as the developing agent. The dropping amount of gold colloidal nanoparticles and the integration time were set and optimized. The limit of detection of the simultaneous detection method of SERS was 10–100 mg/L. Furthermore, SERS was preprocessed by Gap-Segment 2nd Derivative, and then the PCA-LDA model was established, and the model's prediction accuracy can reach 100%. The method is simple, rapid, sensitive and accurate, and has low experimental instruments and equipment requirements. It is suitable for the on-site simultaneous detection of various anti-inflammatory and analgesic chemical drugs in health foods. It can also provide guarantee and support for the establishment of appropriate rapid detection methods and the development of supervision technology.
研究领域:
分析化学
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药物分析
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食品安全
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Remah Sobhy,Jianing Zhang,Zhihua Li,Xiaobo Zou,Asad Nawaz,Noman Walayat,Salim Manoharadas,Yangyang Jia,Mahmoud Salah
We aimed at tabulating and characterizing a flavan-3-ol isolated from date seeds into date seed pills (DSP) as a commercial healthcare pill, and deeply studying their anti-glycoxidation impacts on bovine serum albumin using multi-dimensional approaches SDS-PAGE/NBT, UPLC-ESI-Q-TOF-MS, antiradical via Fenton-type reaction, multispectral, and molecular docking. Results showed that DSP were successfully tabulated (an encapsulation efficiency of 98.78 %) with high antioxidant values via noncovalently binding with whey proteins supported by FTIR and lib-dock results. DSP dose-dependently decayed fluorescent-AGEs by 16.77, 5.32, and 37.41 % in DSP500-BSA-G/MGO/GO models, respectively, later confirmed by SDS-PAGE and initial precursors mitigation results. The docking results confirmed that DSP could bind with glycoxidation sites of BSA-subunits, namely Lys431, Arg458, and Arg196 via H-bonds. DSP trapped both di-carbonyls and reduced CML-level, where new adducts such as epicatechin-mono/MGO-GO, were identified. 500 µg mL −1 DSP reduced OH-induced fluorescence intensity of AGEs by 43.32 % in BSA-G/Fe 2+ /H 2 O 2 . Our results open a new direction for upcycling by-products as effective glycoxidation inhibitors in a type of healthcare pills in protein-rich models.
研究领域:
药学
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生物化学
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营养保健品
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qian-Da Xu,Zhi-Long Yu,Qiang He,Wei-Cai Zeng
A ternary complex composed of soybean protein isolated (SPI), tannic acid (TA) and magnesium ion (M) was established to enhance the capability of protein carriers for TA delivery. SPI was firstly covalently bind with TA (TA-SPI) and then M was employed to form the ternary complex (M-TA-SPI). Their structures, gel and digestion properties were further investigated. TA was observed to covalently bind with SPI. TA-SPI and M-TA-SPI complexes showed different molecule size and spatial structures after binding with M and TA. The increasing of TA amount changed the intramolecular interactions, microstructure and texture properties of M-TA-SPI gels. Compared with TA-SPI, M retarded the gastric digestion of M-TA-SPI and caused higher TA release amount in intestinal tract. In this study, M-TA-SPI was determined to be a good carrier to protect and release TA in gastrointestinal digestion. This kind of complex may have potential applications for loading polyphenols in nutraceuticals.
研究领域:
食品科学
·
营养学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）